Reviewed, UniProtKB/Swiss-Prot P34105 (MAOX_POPTR)

Names and origin

Protein names	Recommended name:     NADP-dependent malic enzyme       Short name=NADP-ME     EC=1.1.1.40
Organism	Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa)
Taxonomic identifier	3694 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Malpighiales › Salicaceae › Saliceae › Populus

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	(S)-malate + NADP+ = pyruvate + CO2 + NADPH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Potential.
Tissue specificity	mRNA found twofold higher in leaves and stems than in roots.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 591	591	NADP-dependent malic enzyme		PRO_0000160203
Regions
	Nucleotide binding	335 – 351	17	NADP By similarity
Sites
	Active site	139	1	Proton donor By similarity
	Active site	210	1	Proton acceptor By similarity
	Metal binding	282	1	Divalent metal cation By similarity
	Metal binding	283	1	Divalent metal cation By similarity
	Metal binding	306	1	Divalent metal cation By similarity
	Binding site	192	1	NAD By similarity
	Binding site	306	1	NAD By similarity
	Binding site	447	1	NAD By similarity
	Site	306	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P34105-1 [UniParc]. Last modified February 1, 1996. Version 3. Checksum: 18DF2433610F4F5A	FASTA	591	65,223
	10 20 30 40 50 60 MESTLKEMRD GASVLDMDPK STVGGGVEDV YGEDRATEDQ LVTPWTISVA SGYTLLRDPH 70 80 90 100 110 120 HNKGLAFTEK ERDAHYLRGL LPPTTISQQL QEKKLMNTIR QYQLPLQKYT AMMELEERNE 130 140 150 160 170 180 RLFYKLLIDN VEELLPVVYT PTVGEACQKY GSIFKRPQGL YISLKEKGKV LDVLKNWPQK 190 200 210 220 230 240 SIQVIVVTDG ERILGLGDLG CQGIGIPVGK LSLYTALGGV RPSACLPVTI DVGTNNEQLL 250 260 270 280 290 300 KDEFYIGLRQ RRATGQEYSE LLHEFMTAVK QNYGEKVLIQ FEDFANHNAF DLLAKYGTTH 310 320 330 340 350 360 LVFNDDIQGT AAVVLAGLIS ALKLLGGSLA DHTFLFLGAG EAGTGIAELI ALEMSRRSKT 370 380 390 400 410 420 PLEETRKKIW LTDSKGLIVS SRKESLQHFK KPWAHEHEPV KGLLEVVKAI KPIVLIGTSG 430 440 450 460 470 480 VGKTFTKEVI EAMASFNEKP LILALSNPTS QSECTAQEAY TWTKGKAIFA SGSPFDPVEY 490 500 510 520 530 540 EGKVFVPGQS NNAYIFPGLG LGLVISGAIR VHDDMLLAAA EALAGQIKEE YLAKGLIYPP 550 560 570 580 590 LSNIRKISVQ IAANVAAKAY ELGLATRLPR PENLVKHAES CMYSPAYRYY R

References

[1]	"Nucleotide sequence of a cDNA encoding malic enzyme from poplar." van Doorsselaere J., Villarroel R., van Montagu M., Inze D. Plant Physiol. 96:1385-1386(1991) [PubMed: 16668348] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X56233 mRNA. Translation: CAA39690.1.
PIR	S18826.
3D structure databases
HSSP	HSSP built from PDB template 1GQ2 based on UniProtKB P40927.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.40. 262297.
Family and domain databases
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAOX_POPTR

Accession

Primary (citable) accession number: P34105

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 60 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)