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P34098

- MANA_DICDI

UniProt

P34098 - MANA_DICDI

Protein

Lysosomal alpha-mannosidase

Gene

manA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 2 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi51 – 511ZincBy similarity
    Metal bindingi53 – 531ZincBy similarity
    Active sitei173 – 1731NucleophileBy similarity
    Metal bindingi173 – 1731ZincBy similarity
    Metal bindingi420 – 4201ZincBy similarity
    Sitei507 – 5082Cleavage; to produce 60 kDa subunitSequence Analysis
    Sitei589 – 5902Cleavage; to produce 58 and 82 kDa subunits; alternateCurated
    Sitei593 – 5942Cleavage; to produce 58 and 82 kDa subunits; alternateCurated

    GO - Molecular functioni

    1. alpha-mannosidase activity Source: dictyBase
    2. carbohydrate binding Source: InterPro
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cellular protein modification process Source: dictyBase
    2. mannose metabolic process Source: InterPro
    3. protein deglycosylation Source: dictyBase
    4. sorocarp development Source: dictyBase

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    CAZyiGH38. Glycoside Hydrolase Family 38.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lysosomal alpha-mannosidase (EC:3.2.1.24)
    Short name:
    Laman
    Alternative name(s):
    Alpha-D-mannoside mannohydrolase
    Alpha-mannosidase A
    Cleaved into the following 2 chains:
    Gene namesi
    Name:manA
    ORF Names:DDB_G0292206
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0292206. manA.

    Subcellular locationi

    Chain Alpha-mannosidase 60 kDa subunit : Lysosome
    Note: A few precursors are secreted. The mature 58 and 60 kDa subunits are lysosomal.

    GO - Cellular componenti

    1. lysosome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Lysosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Propeptidei23 – 4018Pro ISequence AnalysisPRO_0000012081Add
    BLAST
    Chaini41 – 507467Alpha-mannosidase 60 kDa subunitPRO_0000012082Add
    BLAST
    Propeptidei508 – 59588Pro IISequence AnalysisPRO_0000012083Add
    BLAST
    Chaini596 – 1010415Alpha-mannosidase 58 kDa subunitPRO_0000012084Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi68 – 681N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi480 – 4801N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi520 – 5201N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi528 – 5281N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi539 – 5391N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi623 – 6231N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi760 – 7601N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi784 – 7841N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi828 – 8281N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi954 – 9541N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi963 – 9631N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    First cleaved into the mature 58 kDa subunit and an intermediate 82 kDa subunit. The latter is then cleaved to its mature 60 kDa subunit form. These events occur in multiple intracellular compartments. The 60 kDa subunit may form one or more intramolecular disulfide bonds.

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PRIDEiP34098.

    Expressioni

    Inductioni

    Via a protein termed prestarvation factor which is produced and secreted by both growing and developing cells.

    Interactioni

    Subunit structurei

    Tetramer of equimolar amounts of 60 and 58 kDa subunits.

    Protein-protein interaction databases

    STRINGi44689.DDB_0201569.

    Structurei

    3D structure databases

    ProteinModelPortaliP34098.
    SMRiP34098. Positions 32-314.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 38 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG306356.
    KOiK12311.
    OMAiENIYQID.
    PhylomeDBiP34098.

    Family and domain databases

    Gene3Di1.20.1270.50. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.110.10. 1 hit.
    InterProiIPR011013. Gal_mutarotase_SF_dom.
    IPR011330. Glyco_hydro/deAcase_b/a-brl.
    IPR013780. Glyco_hydro_13_b.
    IPR027291. Glyco_hydro_38/57_N.
    IPR011682. Glyco_hydro_38_C.
    IPR015341. Glyco_hydro_38_cen.
    IPR000602. Glyco_hydro_38_N.
    IPR028995. Glyco_hydro_57/38_cen.
    [Graphical view]
    PfamiPF09261. Alpha-mann_mid. 1 hit.
    PF01074. Glyco_hydro_38. 1 hit.
    PF07748. Glyco_hydro_38C. 1 hit.
    [Graphical view]
    SMARTiSM00872. Alpha-mann_mid. 1 hit.
    [Graphical view]
    SUPFAMiSSF74650. SSF74650. 1 hit.
    SSF88688. SSF88688. 1 hit.
    SSF88713. SSF88713. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P34098-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVIKKLFILI FCLFLIINEI NGKKTKINDI KKSKPKLSST LLNVHIVAHT     50
    HDDVGWLKTV DEYYYGSNMS IAFAGVQYTL DTAITCLLAN PERKFIYVEI 100
    AFFQRWWDEQ STTMQNIVKG LVESGQLEFI NGGYCMNDEA TTYYDDTIDQ 150
    MTLGHQFLWE NFGVMPKIGW HIDPFGHSAT QARIFGQLGF DAFIIGRMDY 200
    QDIEARLENK QMEFMWRSTQ STPENQVFTS VLRAMYCTPD GFNFEQGDDP 250
    IQDDPNLFDN NVDSRAEQFT QVALEYATHY RTNNVLIPFG CDFAYLNAQM 300
    YYKNIDKLIA HINSNPDKYG LNLLYSTPSI YIDAVNDANL VWEVKTDDLF 350
    PYADNEFSYW TGYFVSRPAL KGYVRQNNAL LHVVEQMLVT SSNLMPSSRS 400
    EQLVDDIVIM REVMGIAQHH DAVSGTEQQH VADDYAERLS IGNCASLETI 450
    NTVVGTLLTA NGNSKSAAAT PTISFCPLLN QSICPATDPL SSGTSVPVLI 500
    YNSLSWTRNE PVRIPIPIAN VTVTSSSNGS ITSQVNQING TFILEFLATI 550
    PPLGYSTYII TSTASDFVEP NSIPAIIIQD EIIVSGGGKI NEKVSYNDPI 600
    ILENDYINVQ FSSQDGSILS ITNKTSGVTS SITQEYIWYN PSVGNDDSAQ 650
    CSGAYIFRPV EDFAYPYNNA TPSVSIIRGE ISSSIRRFWS NEMVQTFRLY 700
    SNADHLEVEE IIGPIDISDG IGKEIVSRYT TTLVTDQTWY SDSQGMEMQK 750
    RITNYRPSWN LTVVQPTSGN YVPVNAIAYI QDPNQSLQFT IVTDRSRGCA 800
    SLRDGQLDMM MHRRTLKDDG RGVGQPMNES TQIVTTSKLI FHDISSYAQS 850
    HYRPAALSLS HPLLPMFTTT QQSSNDWNSQ YQGVYSPLTS ASPLPNGLKI 900
    QTLQWLDNQD NTILLRIENI YQIDGQDSQD PQTITLDLST IFSTITITSA 950
    TEMNLTGVQK LSNLSRLKWK TVDGKNYDHK SSSSTKEDSS NGFVFTFSPM 1000
    QIRTFIITTN 1010
    Length:1,010
    Mass (Da):113,429
    Last modified:December 4, 2007 - v2
    Checksum:i80C65C941232573F
    GO

    Sequence cautioni

    The sequence AAA33224.1 differs from that shown. Reason: Frameshift at positions 123, 128 and 1001.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti514 – 5152IP → TL in AAA33224. (PubMed:1740448)Curated
    Sequence conflicti978 – 9781D → Y in AAA33224. (PubMed:1740448)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M82822 Genomic DNA. Translation: AAA33224.1. Frameshift.
    AAFI02000188 Genomic DNA. Translation: EAL61313.1.
    PIRiA42265.
    RefSeqiXP_629747.1. XM_629745.1.

    Genome annotation databases

    EnsemblProtistsiDDB0201569; DDB0201569; DDB_G0292206.
    GeneIDi8628576.
    KEGGiddi:DDB_G0292206.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M82822 Genomic DNA. Translation: AAA33224.1 . Frameshift.
    AAFI02000188 Genomic DNA. Translation: EAL61313.1 .
    PIRi A42265.
    RefSeqi XP_629747.1. XM_629745.1.

    3D structure databases

    ProteinModelPortali P34098.
    SMRi P34098. Positions 32-314.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0201569.

    Protein family/group databases

    CAZyi GH38. Glycoside Hydrolase Family 38.

    Proteomic databases

    PRIDEi P34098.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0201569 ; DDB0201569 ; DDB_G0292206 .
    GeneIDi 8628576.
    KEGGi ddi:DDB_G0292206.

    Organism-specific databases

    dictyBasei DDB_G0292206. manA.

    Phylogenomic databases

    eggNOGi NOG306356.
    KOi K12311.
    OMAi ENIYQID.
    PhylomeDBi P34098.

    Miscellaneous databases

    PROi P34098.

    Family and domain databases

    Gene3Di 1.20.1270.50. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.110.10. 1 hit.
    InterProi IPR011013. Gal_mutarotase_SF_dom.
    IPR011330. Glyco_hydro/deAcase_b/a-brl.
    IPR013780. Glyco_hydro_13_b.
    IPR027291. Glyco_hydro_38/57_N.
    IPR011682. Glyco_hydro_38_C.
    IPR015341. Glyco_hydro_38_cen.
    IPR000602. Glyco_hydro_38_N.
    IPR028995. Glyco_hydro_57/38_cen.
    [Graphical view ]
    Pfami PF09261. Alpha-mann_mid. 1 hit.
    PF01074. Glyco_hydro_38. 1 hit.
    PF07748. Glyco_hydro_38C. 1 hit.
    [Graphical view ]
    SMARTi SM00872. Alpha-mann_mid. 1 hit.
    [Graphical view ]
    SUPFAMi SSF74650. SSF74650. 1 hit.
    SSF88688. SSF88688. 1 hit.
    SSF88713. SSF88713. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of the structural gene coding for the developmentally regulated lysosomal enzyme, alpha-mannosidase, in Dictyostelium discoideum."
      Schatzle J., Bush J., Cardelli J.
      J. Biol. Chem. 267:4000-4007(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 41-63 AND 596-612.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiMANA_DICDI
    AccessioniPrimary (citable) accession number: P34098
    Secondary accession number(s): Q54DI0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 102 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3