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P34098

- MANA_DICDI

UniProt

P34098 - MANA_DICDI

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Protein

Lysosomal alpha-mannosidase

Gene
manA, DDB_G0292206
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi51 – 511Zinc By similarity
Metal bindingi53 – 531Zinc By similarity
Active sitei173 – 1731Nucleophile By similarity
Metal bindingi173 – 1731Zinc By similarity
Metal bindingi420 – 4201Zinc By similarity
Sitei507 – 5082Cleavage; to produce 60 kDa subunit Reviewed prediction
Sitei589 – 5902Cleavage; to produce 58 and 82 kDa subunits; alternate Inferred
Sitei593 – 5942Cleavage; to produce 58 and 82 kDa subunits; alternate Inferred

GO - Molecular functioni

  1. alpha-mannosidase activity Source: dictyBase
  2. carbohydrate binding Source: InterPro
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. cellular protein modification process Source: dictyBase
  2. mannose metabolic process Source: InterPro
  3. protein deglycosylation Source: dictyBase
  4. sorocarp development Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

CAZyiGH38. Glycoside Hydrolase Family 38.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal alpha-mannosidase (EC:3.2.1.24)
Short name:
Laman
Alternative name(s):
Alpha-D-mannoside mannohydrolase
Alpha-mannosidase A
Cleaved into the following 2 chains:
Gene namesi
Name:manA
ORF Names:DDB_G0292206
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0292206. manA.

Subcellular locationi

Chain Alpha-mannosidase 60 kDa subunit : Lysosome
Note: A few precursors are secreted. The mature 58 and 60 kDa subunits are lysosomal.

GO - Cellular componenti

  1. lysosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222 Reviewed predictionAdd
BLAST
Propeptidei23 – 4018Pro I Reviewed predictionPRO_0000012081Add
BLAST
Chaini41 – 507467Alpha-mannosidase 60 kDa subunitPRO_0000012082Add
BLAST
Propeptidei508 – 59588Pro II Reviewed predictionPRO_0000012083Add
BLAST
Chaini596 – 1010415Alpha-mannosidase 58 kDa subunitPRO_0000012084Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi68 – 681N-linked (GlcNAc...) Reviewed prediction
Glycosylationi480 – 4801N-linked (GlcNAc...) Reviewed prediction
Glycosylationi520 – 5201N-linked (GlcNAc...) Reviewed prediction
Glycosylationi528 – 5281N-linked (GlcNAc...) Reviewed prediction
Glycosylationi539 – 5391N-linked (GlcNAc...) Reviewed prediction
Glycosylationi623 – 6231N-linked (GlcNAc...) Reviewed prediction
Glycosylationi760 – 7601N-linked (GlcNAc...) Reviewed prediction
Glycosylationi784 – 7841N-linked (GlcNAc...) Reviewed prediction
Glycosylationi828 – 8281N-linked (GlcNAc...) Reviewed prediction
Glycosylationi954 – 9541N-linked (GlcNAc...) Reviewed prediction
Glycosylationi963 – 9631N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

First cleaved into the mature 58 kDa subunit and an intermediate 82 kDa subunit. The latter is then cleaved to its mature 60 kDa subunit form. These events occur in multiple intracellular compartments. The 60 kDa subunit may form one or more intramolecular disulfide bonds.

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiP34098.

Expressioni

Inductioni

Via a protein termed prestarvation factor which is produced and secreted by both growing and developing cells.

Interactioni

Subunit structurei

Tetramer of equimolar amounts of 60 and 58 kDa subunits.

Protein-protein interaction databases

STRINGi44689.DDB_0201569.

Structurei

3D structure databases

ProteinModelPortaliP34098.
SMRiP34098. Positions 32-314.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG306356.
KOiK12311.
OMAiENIYQID.
PhylomeDBiP34098.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P34098-1 [UniParc]FASTAAdd to Basket

« Hide

MVIKKLFILI FCLFLIINEI NGKKTKINDI KKSKPKLSST LLNVHIVAHT     50
HDDVGWLKTV DEYYYGSNMS IAFAGVQYTL DTAITCLLAN PERKFIYVEI 100
AFFQRWWDEQ STTMQNIVKG LVESGQLEFI NGGYCMNDEA TTYYDDTIDQ 150
MTLGHQFLWE NFGVMPKIGW HIDPFGHSAT QARIFGQLGF DAFIIGRMDY 200
QDIEARLENK QMEFMWRSTQ STPENQVFTS VLRAMYCTPD GFNFEQGDDP 250
IQDDPNLFDN NVDSRAEQFT QVALEYATHY RTNNVLIPFG CDFAYLNAQM 300
YYKNIDKLIA HINSNPDKYG LNLLYSTPSI YIDAVNDANL VWEVKTDDLF 350
PYADNEFSYW TGYFVSRPAL KGYVRQNNAL LHVVEQMLVT SSNLMPSSRS 400
EQLVDDIVIM REVMGIAQHH DAVSGTEQQH VADDYAERLS IGNCASLETI 450
NTVVGTLLTA NGNSKSAAAT PTISFCPLLN QSICPATDPL SSGTSVPVLI 500
YNSLSWTRNE PVRIPIPIAN VTVTSSSNGS ITSQVNQING TFILEFLATI 550
PPLGYSTYII TSTASDFVEP NSIPAIIIQD EIIVSGGGKI NEKVSYNDPI 600
ILENDYINVQ FSSQDGSILS ITNKTSGVTS SITQEYIWYN PSVGNDDSAQ 650
CSGAYIFRPV EDFAYPYNNA TPSVSIIRGE ISSSIRRFWS NEMVQTFRLY 700
SNADHLEVEE IIGPIDISDG IGKEIVSRYT TTLVTDQTWY SDSQGMEMQK 750
RITNYRPSWN LTVVQPTSGN YVPVNAIAYI QDPNQSLQFT IVTDRSRGCA 800
SLRDGQLDMM MHRRTLKDDG RGVGQPMNES TQIVTTSKLI FHDISSYAQS 850
HYRPAALSLS HPLLPMFTTT QQSSNDWNSQ YQGVYSPLTS ASPLPNGLKI 900
QTLQWLDNQD NTILLRIENI YQIDGQDSQD PQTITLDLST IFSTITITSA 950
TEMNLTGVQK LSNLSRLKWK TVDGKNYDHK SSSSTKEDSS NGFVFTFSPM 1000
QIRTFIITTN 1010
Length:1,010
Mass (Da):113,429
Last modified:December 4, 2007 - v2
Checksum:i80C65C941232573F
GO

Sequence cautioni

The sequence AAA33224.1 differs from that shown. Reason: Frameshift at positions 123, 128 and 1001.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti514 – 5152IP → TL in AAA33224. 1 Publication
Sequence conflicti978 – 9781D → Y in AAA33224. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M82822 Genomic DNA. Translation: AAA33224.1. Frameshift.
AAFI02000188 Genomic DNA. Translation: EAL61313.1.
PIRiA42265.
RefSeqiXP_629747.1. XM_629745.1.

Genome annotation databases

EnsemblProtistsiDDB0201569; DDB0201569; DDB_G0292206.
GeneIDi8628576.
KEGGiddi:DDB_G0292206.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M82822 Genomic DNA. Translation: AAA33224.1 . Frameshift.
AAFI02000188 Genomic DNA. Translation: EAL61313.1 .
PIRi A42265.
RefSeqi XP_629747.1. XM_629745.1.

3D structure databases

ProteinModelPortali P34098.
SMRi P34098. Positions 32-314.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0201569.

Protein family/group databases

CAZyi GH38. Glycoside Hydrolase Family 38.

Proteomic databases

PRIDEi P34098.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0201569 ; DDB0201569 ; DDB_G0292206 .
GeneIDi 8628576.
KEGGi ddi:DDB_G0292206.

Organism-specific databases

dictyBasei DDB_G0292206. manA.

Phylogenomic databases

eggNOGi NOG306356.
KOi K12311.
OMAi ENIYQID.
PhylomeDBi P34098.

Miscellaneous databases

PROi P34098.

Family and domain databases

Gene3Di 1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view ]
Pfami PF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view ]
SMARTi SM00872. Alpha-mann_mid. 1 hit.
[Graphical view ]
SUPFAMi SSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of the structural gene coding for the developmentally regulated lysosomal enzyme, alpha-mannosidase, in Dictyostelium discoideum."
    Schatzle J., Bush J., Cardelli J.
    J. Biol. Chem. 267:4000-4007(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 41-63 AND 596-612.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiMANA_DICDI
AccessioniPrimary (citable) accession number: P34098
Secondary accession number(s): Q54DI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: December 4, 2007
Last modified: September 3, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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