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P34098

- MANA_DICDI

UniProt

P34098 - MANA_DICDI

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Protein

Lysosomal alpha-mannosidase

Gene

manA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactori

Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi51 – 511ZincBy similarity
Metal bindingi53 – 531ZincBy similarity
Active sitei173 – 1731NucleophileBy similarity
Metal bindingi173 – 1731ZincBy similarity
Metal bindingi420 – 4201ZincBy similarity
Sitei507 – 5082Cleavage; to produce 60 kDa subunitSequence Analysis
Sitei589 – 5902Cleavage; to produce 58 and 82 kDa subunits; alternateCurated
Sitei593 – 5942Cleavage; to produce 58 and 82 kDa subunits; alternateCurated

GO - Molecular functioni

  1. alpha-mannosidase activity Source: dictyBase
  2. carbohydrate binding Source: InterPro
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. cellular protein modification process Source: dictyBase
  2. mannose metabolic process Source: InterPro
  3. protein deglycosylation Source: dictyBase
  4. sorocarp development Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

CAZyiGH38. Glycoside Hydrolase Family 38.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal alpha-mannosidase (EC:3.2.1.24)
Short name:
Laman
Alternative name(s):
Alpha-D-mannoside mannohydrolase
Alpha-mannosidase A
Cleaved into the following 2 chains:
Gene namesi
Name:manA
ORF Names:DDB_G0292206
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0292206. manA.

Subcellular locationi

Chain Alpha-mannosidase 60 kDa subunit : Lysosome
Note: A few precursors are secreted. The mature 58 and 60 kDa subunits are lysosomal.

GO - Cellular componenti

  1. lysosome Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Propeptidei23 – 4018Pro ISequence AnalysisPRO_0000012081Add
BLAST
Chaini41 – 507467Alpha-mannosidase 60 kDa subunitPRO_0000012082Add
BLAST
Propeptidei508 – 59588Pro IISequence AnalysisPRO_0000012083Add
BLAST
Chaini596 – 1010415Alpha-mannosidase 58 kDa subunitPRO_0000012084Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi68 – 681N-linked (GlcNAc...)Sequence Analysis
Glycosylationi480 – 4801N-linked (GlcNAc...)Sequence Analysis
Glycosylationi520 – 5201N-linked (GlcNAc...)Sequence Analysis
Glycosylationi528 – 5281N-linked (GlcNAc...)Sequence Analysis
Glycosylationi539 – 5391N-linked (GlcNAc...)Sequence Analysis
Glycosylationi623 – 6231N-linked (GlcNAc...)Sequence Analysis
Glycosylationi760 – 7601N-linked (GlcNAc...)Sequence Analysis
Glycosylationi784 – 7841N-linked (GlcNAc...)Sequence Analysis
Glycosylationi828 – 8281N-linked (GlcNAc...)Sequence Analysis
Glycosylationi954 – 9541N-linked (GlcNAc...)Sequence Analysis
Glycosylationi963 – 9631N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

First cleaved into the mature 58 kDa subunit and an intermediate 82 kDa subunit. The latter is then cleaved to its mature 60 kDa subunit form. These events occur in multiple intracellular compartments. The 60 kDa subunit may form one or more intramolecular disulfide bonds.

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiP34098.

Expressioni

Inductioni

Via a protein termed prestarvation factor which is produced and secreted by both growing and developing cells.

Interactioni

Subunit structurei

Tetramer of equimolar amounts of 60 and 58 kDa subunits.

Protein-protein interaction databases

STRINGi44689.DDB_0201569.

Structurei

3D structure databases

ProteinModelPortaliP34098.
SMRiP34098. Positions 32-314.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 38 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG306356.
InParanoidiP34098.
KOiK12311.
OMAiENIYQID.
PhylomeDBiP34098.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P34098-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVIKKLFILI FCLFLIINEI NGKKTKINDI KKSKPKLSST LLNVHIVAHT
60 70 80 90 100
HDDVGWLKTV DEYYYGSNMS IAFAGVQYTL DTAITCLLAN PERKFIYVEI
110 120 130 140 150
AFFQRWWDEQ STTMQNIVKG LVESGQLEFI NGGYCMNDEA TTYYDDTIDQ
160 170 180 190 200
MTLGHQFLWE NFGVMPKIGW HIDPFGHSAT QARIFGQLGF DAFIIGRMDY
210 220 230 240 250
QDIEARLENK QMEFMWRSTQ STPENQVFTS VLRAMYCTPD GFNFEQGDDP
260 270 280 290 300
IQDDPNLFDN NVDSRAEQFT QVALEYATHY RTNNVLIPFG CDFAYLNAQM
310 320 330 340 350
YYKNIDKLIA HINSNPDKYG LNLLYSTPSI YIDAVNDANL VWEVKTDDLF
360 370 380 390 400
PYADNEFSYW TGYFVSRPAL KGYVRQNNAL LHVVEQMLVT SSNLMPSSRS
410 420 430 440 450
EQLVDDIVIM REVMGIAQHH DAVSGTEQQH VADDYAERLS IGNCASLETI
460 470 480 490 500
NTVVGTLLTA NGNSKSAAAT PTISFCPLLN QSICPATDPL SSGTSVPVLI
510 520 530 540 550
YNSLSWTRNE PVRIPIPIAN VTVTSSSNGS ITSQVNQING TFILEFLATI
560 570 580 590 600
PPLGYSTYII TSTASDFVEP NSIPAIIIQD EIIVSGGGKI NEKVSYNDPI
610 620 630 640 650
ILENDYINVQ FSSQDGSILS ITNKTSGVTS SITQEYIWYN PSVGNDDSAQ
660 670 680 690 700
CSGAYIFRPV EDFAYPYNNA TPSVSIIRGE ISSSIRRFWS NEMVQTFRLY
710 720 730 740 750
SNADHLEVEE IIGPIDISDG IGKEIVSRYT TTLVTDQTWY SDSQGMEMQK
760 770 780 790 800
RITNYRPSWN LTVVQPTSGN YVPVNAIAYI QDPNQSLQFT IVTDRSRGCA
810 820 830 840 850
SLRDGQLDMM MHRRTLKDDG RGVGQPMNES TQIVTTSKLI FHDISSYAQS
860 870 880 890 900
HYRPAALSLS HPLLPMFTTT QQSSNDWNSQ YQGVYSPLTS ASPLPNGLKI
910 920 930 940 950
QTLQWLDNQD NTILLRIENI YQIDGQDSQD PQTITLDLST IFSTITITSA
960 970 980 990 1000
TEMNLTGVQK LSNLSRLKWK TVDGKNYDHK SSSSTKEDSS NGFVFTFSPM
1010
QIRTFIITTN
Length:1,010
Mass (Da):113,429
Last modified:December 4, 2007 - v2
Checksum:i80C65C941232573F
GO

Sequence cautioni

The sequence AAA33224.1 differs from that shown. Reason: Frameshift at positions 123, 128 and 1001.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti514 – 5152IP → TL in AAA33224. (PubMed:1740448)Curated
Sequence conflicti978 – 9781D → Y in AAA33224. (PubMed:1740448)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M82822 Genomic DNA. Translation: AAA33224.1. Frameshift.
AAFI02000188 Genomic DNA. Translation: EAL61313.1.
PIRiA42265.
RefSeqiXP_629747.1. XM_629745.1.

Genome annotation databases

EnsemblProtistsiDDB0201569; DDB0201569; DDB_G0292206.
GeneIDi8628576.
KEGGiddi:DDB_G0292206.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M82822 Genomic DNA. Translation: AAA33224.1 . Frameshift.
AAFI02000188 Genomic DNA. Translation: EAL61313.1 .
PIRi A42265.
RefSeqi XP_629747.1. XM_629745.1.

3D structure databases

ProteinModelPortali P34098.
SMRi P34098. Positions 32-314.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0201569.

Protein family/group databases

CAZyi GH38. Glycoside Hydrolase Family 38.

Proteomic databases

PRIDEi P34098.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0201569 ; DDB0201569 ; DDB_G0292206 .
GeneIDi 8628576.
KEGGi ddi:DDB_G0292206.

Organism-specific databases

dictyBasei DDB_G0292206. manA.

Phylogenomic databases

eggNOGi NOG306356.
InParanoidi P34098.
KOi K12311.
OMAi ENIYQID.
PhylomeDBi P34098.

Miscellaneous databases

PROi P34098.

Family and domain databases

Gene3Di 1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view ]
Pfami PF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view ]
SMARTi SM00872. Alpha-mann_mid. 1 hit.
[Graphical view ]
SUPFAMi SSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of the structural gene coding for the developmentally regulated lysosomal enzyme, alpha-mannosidase, in Dictyostelium discoideum."
    Schatzle J., Bush J., Cardelli J.
    J. Biol. Chem. 267:4000-4007(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 41-63 AND 596-612.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiMANA_DICDI
AccessioniPrimary (citable) accession number: P34098
Secondary accession number(s): Q54DI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: December 4, 2007
Last modified: October 29, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3