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Protein

Ribonuclease 4

Gene

RNASE4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This RNase has marked specificity towards the 3' side of uridine nucleotides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381Substrate
Active sitei40 – 401Proton acceptor
Binding sitei93 – 931Substrate
Binding sitei110 – 1101Substrate
Active sitei144 – 1441Proton donor

GO - Molecular functioni

  1. endonuclease activity Source: GO_Central
  2. nucleic acid binding Source: InterPro
  3. pancreatic ribonuclease activity Source: UniProtKB
  4. ribonuclease activity Source: GO_Central

GO - Biological processi

  1. cellular response to starvation Source: Ensembl
  2. mRNA cleavage Source: UniProtKB
  3. RNA phosphodiester bond hydrolysis Source: GOC
  4. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 4 (EC:3.1.27.-)
Short name:
RNase 4
Gene namesi
Name:RNASE4
Synonyms:RNS4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:10047. RNASE4.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
  2. extracellular vesicular exosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34415.

Polymorphism and mutation databases

BioMutaiRNASE4.
DMDMi1710614.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 28281 PublicationAdd
BLAST
Chaini29 – 147119Ribonuclease 4PRO_0000030883Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei29 – 291Pyrrolidone carboxylic acid1 Publication
Disulfide bondi53 ↔ 1091 Publication
Disulfide bondi67 ↔ 1201 Publication
Disulfide bondi85 ↔ 1351 Publication
Disulfide bondi92 ↔ 991 Publication

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

MaxQBiP34096.
PaxDbiP34096.
PeptideAtlasiP34096.
PRIDEiP34096.

Expressioni

Gene expression databases

BgeeiP34096.
ExpressionAtlasiP34096. baseline.
GenevestigatoriP34096.

Interactioni

Protein-protein interaction databases

BioGridi111967. 1 interaction.

Structurei

Secondary structure

1
147
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi29 – 4012Combined sources
Helixi50 – 5910Combined sources
Beta strandi62 – 665Combined sources
Beta strandi69 – 746Combined sources
Helixi78 – 825Combined sources
Helixi83 – 864Combined sources
Beta strandi99 – 11113Combined sources
Beta strandi113 – 1153Combined sources
Beta strandi122 – 13615Combined sources
Turni137 – 1404Combined sources
Beta strandi141 – 1477Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RNFX-ray2.10A/B29-147[»]
2RNFX-ray2.40A/B29-147[»]
ProteinModelPortaliP34096.
SMRiP34096. Positions 29-147.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP34096.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni68 – 725Substrate binding

Sequence similaritiesi

Belongs to the pancreatic ribonuclease family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG42321.
HOGENOMiHOG000276883.
HOVERGENiHBG008396.
InParanoidiP34096.
KOiK16632.
OMAiSSRAPNC.
PhylomeDBiP34096.
TreeFamiTF333393.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P34096-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALQRTHSLL LLLLLTLLGL GLVQPSYGQD GMYQRFLRQH VHPEETGGSD
60 70 80 90 100
RYCNLMMQRR KMTLYHCKRF NTFIHEDIWN IRSICSTTNI QCKNGKMNCH
110 120 130 140
EGVVKVTDCR DTGSSRAPNC RYRAIASTRR VVIACEGNPQ VPVHFDG
Length:147
Mass (Da):16,840
Last modified:October 1, 1996 - v3
Checksum:i4C24FEA249F3EC2F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti54 – 541N → D in AAA96750 (PubMed:7501448).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti16 – 161T → S.
Corresponds to variant rs3748338 [ dbSNP | Ensembl ].
VAR_024618

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37931 mRNA. Translation: BAA07150.1.
BC015520 mRNA. Translation: AAH15520.1.
U36775 Genomic DNA. Translation: AAA96750.1.
CCDSiCCDS9555.1.
PIRiI52489.
RefSeqiNP_001269121.1. NM_001282192.1.
NP_001269122.1. NM_001282193.1.
NP_002928.1. NM_002937.4.
NP_919412.1. NM_194431.2.
UniGeneiHs.283749.

Genome annotation databases

EnsembliENST00000397995; ENSP00000381081; ENSG00000258818.
ENST00000555597; ENSP00000451624; ENSG00000258818.
ENST00000555835; ENSP00000452245; ENSG00000258818.
GeneIDi6038.
KEGGihsa:6038.
UCSCiuc001vxy.4. human.

Polymorphism and mutation databases

BioMutaiRNASE4.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37931 mRNA. Translation: BAA07150.1.
BC015520 mRNA. Translation: AAH15520.1.
U36775 Genomic DNA. Translation: AAA96750.1.
CCDSiCCDS9555.1.
PIRiI52489.
RefSeqiNP_001269121.1. NM_001282192.1.
NP_001269122.1. NM_001282193.1.
NP_002928.1. NM_002937.4.
NP_919412.1. NM_194431.2.
UniGeneiHs.283749.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RNFX-ray2.10A/B29-147[»]
2RNFX-ray2.40A/B29-147[»]
ProteinModelPortaliP34096.
SMRiP34096. Positions 29-147.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111967. 1 interaction.

Polymorphism and mutation databases

BioMutaiRNASE4.
DMDMi1710614.

Proteomic databases

MaxQBiP34096.
PaxDbiP34096.
PeptideAtlasiP34096.
PRIDEiP34096.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000397995; ENSP00000381081; ENSG00000258818.
ENST00000555597; ENSP00000451624; ENSG00000258818.
ENST00000555835; ENSP00000452245; ENSG00000258818.
GeneIDi6038.
KEGGihsa:6038.
UCSCiuc001vxy.4. human.

Organism-specific databases

CTDi6038.
GeneCardsiGC14P021191.
HGNCiHGNC:10047. RNASE4.
MIMi601030. gene.
neXtProtiNX_P34096.
PharmGKBiPA34415.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG42321.
HOGENOMiHOG000276883.
HOVERGENiHBG008396.
InParanoidiP34096.
KOiK16632.
OMAiSSRAPNC.
PhylomeDBiP34096.
TreeFamiTF333393.

Miscellaneous databases

EvolutionaryTraceiP34096.
GeneWikiiRibonuclease_4.
GenomeRNAii6038.
NextBioi23533.
PROiP34096.
SOURCEiSearch...

Gene expression databases

BgeeiP34096.
ExpressionAtlasiP34096. baseline.
GenevestigatoriP34096.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pancreas.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pancreas.
  3. "Human ribonuclease 4 (RNase 4): coding sequence, chromosomal localization and identification of two distinct transcripts in human somatic tissues."
    Rosenberg H.F., Dyer K.D.
    Nucleic Acids Res. 23:4290-4295(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 29-147.
  4. "The amino acid sequence of human ribonuclease 4, a highly conserved ribonuclease that cleaves specifically on the 3' side of uridine."
    Zhou H.-M., Strydom D.J.
    Eur. J. Biochem. 217:401-410(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 29-147, PYROGLUTAMATE FORMATION AT GLN-29, DISULFIDE BONDS.
    Tissue: Plasma.
  5. "Isolation and characterization of a human colon carcinoma-secreted enzyme with pancreatic ribonuclease-like activity."
    Shapiro R., Fett J.W., Strydom D.J., Vallee B.L.
    Biochemistry 25:7255-7264(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "The three-dimensional structure of human RNase 4, unliganded and complexed with d(Up), reveals the basis for its uridine selectivity."
    Terzyan S.S., Peracaula R., de Llorens R., Tsushima Y., Yamada H., Seno M., Gomis-Rueth F.-X., Coll M.
    J. Mol. Biol. 285:205-214(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 29-147.

Entry informationi

Entry nameiRNAS4_HUMAN
AccessioniPrimary (citable) accession number: P34096
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: October 1, 1996
Last modified: April 29, 2015
This is version 147 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.