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P34096

- RNAS4_HUMAN

UniProt

P34096 - RNAS4_HUMAN

Protein

Ribonuclease 4

Gene

RNASE4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    This RNase has marked specificity towards the 3' side of uridine nucleotides.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei38 – 381Substrate
    Active sitei40 – 401Proton acceptor
    Binding sitei93 – 931Substrate
    Binding sitei110 – 1101Substrate
    Active sitei144 – 1441Proton donor

    GO - Molecular functioni

    1. nucleic acid binding Source: InterPro
    2. pancreatic ribonuclease activity Source: UniProtKB

    GO - Biological processi

    1. mRNA cleavage Source: UniProtKB
    2. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease 4 (EC:3.1.27.-)
    Short name:
    RNase 4
    Gene namesi
    Name:RNASE4
    Synonyms:RNS4
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 14

    Organism-specific databases

    HGNCiHGNC:10047. RNASE4.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB
    2. extracellular vesicular exosome Source: UniProt

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA34415.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 28281 PublicationAdd
    BLAST
    Chaini29 – 147119Ribonuclease 4PRO_0000030883Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei29 – 291Pyrrolidone carboxylic acid1 Publication
    Disulfide bondi53 ↔ 1091 Publication
    Disulfide bondi67 ↔ 1201 Publication
    Disulfide bondi85 ↔ 1351 Publication
    Disulfide bondi92 ↔ 991 Publication

    Keywords - PTMi

    Disulfide bond, Pyrrolidone carboxylic acid

    Proteomic databases

    MaxQBiP34096.
    PaxDbiP34096.
    PeptideAtlasiP34096.
    PRIDEiP34096.

    Expressioni

    Gene expression databases

    BgeeiP34096.
    GenevestigatoriP34096.

    Organism-specific databases

    HPAiHPA048300.

    Interactioni

    Protein-protein interaction databases

    BioGridi111967. 1 interaction.

    Structurei

    Secondary structure

    1
    147
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi29 – 4012
    Helixi50 – 5910
    Beta strandi62 – 665
    Beta strandi69 – 746
    Helixi78 – 825
    Helixi83 – 864
    Beta strandi99 – 11113
    Beta strandi113 – 1153
    Beta strandi122 – 13615
    Turni137 – 1404
    Beta strandi141 – 1477

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1RNFX-ray2.10A/B29-147[»]
    2RNFX-ray2.40A/B29-147[»]
    ProteinModelPortaliP34096.
    SMRiP34096. Positions 29-147.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP34096.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni68 – 725Substrate binding

    Sequence similaritiesi

    Belongs to the pancreatic ribonuclease family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG42321.
    HOGENOMiHOG000276883.
    HOVERGENiHBG008396.
    InParanoidiP34096.
    KOiK16632.
    OMAiCHDEITE.
    PhylomeDBiP34096.
    TreeFamiTF333393.

    Family and domain databases

    Gene3Di3.10.130.10. 1 hit.
    InterProiIPR001427. RNaseA.
    IPR023411. RNaseA_AS.
    IPR023412. RNaseA_domain.
    [Graphical view]
    PANTHERiPTHR11437. PTHR11437. 1 hit.
    PfamiPF00074. RnaseA. 1 hit.
    [Graphical view]
    PRINTSiPR00794. RIBONUCLEASE.
    ProDomiPD000535. RNaseA. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00092. RNAse_Pc. 1 hit.
    [Graphical view]
    SUPFAMiSSF54076. SSF54076. 1 hit.
    PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P34096-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALQRTHSLL LLLLLTLLGL GLVQPSYGQD GMYQRFLRQH VHPEETGGSD    50
    RYCNLMMQRR KMTLYHCKRF NTFIHEDIWN IRSICSTTNI QCKNGKMNCH 100
    EGVVKVTDCR DTGSSRAPNC RYRAIASTRR VVIACEGNPQ VPVHFDG 147
    Length:147
    Mass (Da):16,840
    Last modified:October 1, 1996 - v3
    Checksum:i4C24FEA249F3EC2F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti54 – 541N → D in AAA96750. (PubMed:7501448)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti16 – 161T → S.
    Corresponds to variant rs3748338 [ dbSNP | Ensembl ].
    VAR_024618

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D37931 mRNA. Translation: BAA07150.1.
    BC015520 mRNA. Translation: AAH15520.1.
    U36775 Genomic DNA. Translation: AAA96750.1.
    CCDSiCCDS9555.1.
    PIRiI52489.
    RefSeqiNP_001269121.1. NM_001282192.1.
    NP_001269122.1. NM_001282193.1.
    NP_002928.1. NM_002937.4.
    NP_919412.1. NM_194431.2.
    UniGeneiHs.283749.

    Genome annotation databases

    EnsembliENST00000397995; ENSP00000381081; ENSG00000258818.
    ENST00000555597; ENSP00000451624; ENSG00000258818.
    ENST00000555835; ENSP00000452245; ENSG00000258818.
    GeneIDi6038.
    KEGGihsa:6038.
    UCSCiuc001vxy.4. human.

    Polymorphism databases

    DMDMi1710614.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D37931 mRNA. Translation: BAA07150.1 .
    BC015520 mRNA. Translation: AAH15520.1 .
    U36775 Genomic DNA. Translation: AAA96750.1 .
    CCDSi CCDS9555.1.
    PIRi I52489.
    RefSeqi NP_001269121.1. NM_001282192.1.
    NP_001269122.1. NM_001282193.1.
    NP_002928.1. NM_002937.4.
    NP_919412.1. NM_194431.2.
    UniGenei Hs.283749.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1RNF X-ray 2.10 A/B 29-147 [» ]
    2RNF X-ray 2.40 A/B 29-147 [» ]
    ProteinModelPortali P34096.
    SMRi P34096. Positions 29-147.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111967. 1 interaction.

    Polymorphism databases

    DMDMi 1710614.

    Proteomic databases

    MaxQBi P34096.
    PaxDbi P34096.
    PeptideAtlasi P34096.
    PRIDEi P34096.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000397995 ; ENSP00000381081 ; ENSG00000258818 .
    ENST00000555597 ; ENSP00000451624 ; ENSG00000258818 .
    ENST00000555835 ; ENSP00000452245 ; ENSG00000258818 .
    GeneIDi 6038.
    KEGGi hsa:6038.
    UCSCi uc001vxy.4. human.

    Organism-specific databases

    CTDi 6038.
    GeneCardsi GC14P021185.
    HGNCi HGNC:10047. RNASE4.
    HPAi HPA048300.
    MIMi 601030. gene.
    neXtProti NX_P34096.
    PharmGKBi PA34415.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG42321.
    HOGENOMi HOG000276883.
    HOVERGENi HBG008396.
    InParanoidi P34096.
    KOi K16632.
    OMAi CHDEITE.
    PhylomeDBi P34096.
    TreeFami TF333393.

    Miscellaneous databases

    EvolutionaryTracei P34096.
    GeneWikii Ribonuclease_4.
    GenomeRNAii 6038.
    NextBioi 23533.
    PROi P34096.
    SOURCEi Search...

    Gene expression databases

    Bgeei P34096.
    Genevestigatori P34096.

    Family and domain databases

    Gene3Di 3.10.130.10. 1 hit.
    InterProi IPR001427. RNaseA.
    IPR023411. RNaseA_AS.
    IPR023412. RNaseA_domain.
    [Graphical view ]
    PANTHERi PTHR11437. PTHR11437. 1 hit.
    Pfami PF00074. RnaseA. 1 hit.
    [Graphical view ]
    PRINTSi PR00794. RIBONUCLEASE.
    ProDomi PD000535. RNaseA. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00092. RNAse_Pc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54076. SSF54076. 1 hit.
    PROSITEi PS00127. RNASE_PANCREATIC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Pancreas.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Pancreas.
    3. "Human ribonuclease 4 (RNase 4): coding sequence, chromosomal localization and identification of two distinct transcripts in human somatic tissues."
      Rosenberg H.F., Dyer K.D.
      Nucleic Acids Res. 23:4290-4295(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 29-147.
    4. "The amino acid sequence of human ribonuclease 4, a highly conserved ribonuclease that cleaves specifically on the 3' side of uridine."
      Zhou H.-M., Strydom D.J.
      Eur. J. Biochem. 217:401-410(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 29-147, PYROGLUTAMATE FORMATION AT GLN-29, DISULFIDE BONDS.
      Tissue: Plasma.
    5. "Isolation and characterization of a human colon carcinoma-secreted enzyme with pancreatic ribonuclease-like activity."
      Shapiro R., Fett J.W., Strydom D.J., Vallee B.L.
      Biochemistry 25:7255-7264(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. "The three-dimensional structure of human RNase 4, unliganded and complexed with d(Up), reveals the basis for its uridine selectivity."
      Terzyan S.S., Peracaula R., de Llorens R., Tsushima Y., Yamada H., Seno M., Gomis-Rueth F.-X., Coll M.
      J. Mol. Biol. 285:205-214(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 29-147.

    Entry informationi

    Entry nameiRNAS4_HUMAN
    AccessioniPrimary (citable) accession number: P34096
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 142 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 14
      Human chromosome 14: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3