Ribonuclease 4 precursor - Homo sapiens (Human)

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P34096 (RNAS4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 129. History...

Clusters with 100%, 90%, 50% identity |

Documents (6) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Ribonuclease 4
Short name=RNase 4
EC=3.1.27.-

Gene names

Name:	RNASE4
Synonyms:	RNS4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This RNase has marked specificity towards the 3' side of uridine nucleotides.
Subcellular location	Secreted.
Sequence similarities	Belongs to the pancreatic ribonuclease family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond Pyrrolidone carboxylic acid
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	mRNA cleavage Non-traceable author statement Ref.1. Source: UniProtKB
Cellular_component	extracellular region Inferred from direct assay Ref.5. Source: UniProtKB
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro pancreatic ribonuclease activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 28

Ref.4

Chain

29 – 147

119

Ribonuclease 4

PRO_0000030883

Regions

Region

68 – 72

Substrate binding

Sites

Active site

Proton acceptor

Active site

144

Proton donor

Binding site

Substrate

Binding site

Substrate

Binding site

110

Substrate

Amino acid modifications

Modified residue

Pyrrolidone carboxylic acid

Disulfide bond

Disulfide bond

Disulfide bond

Disulfide bond

Natural variations

Natural variant

T → S.
Corresponds to variant rs3748338 [ dbSNP | Ensembl ].

VAR_024618

Experimental info

Sequence conflict

N → D in AAA96750. Ref.3

Secondary structure

147

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P34096 [UniParc].

Last modified October 1, 1996. Version 3.
Checksum: 4C24FEA249F3EC2F
Show »

FASTA

147

16,840

        10         20         30         40         50         60 
MALQRTHSLL LLLLLTLLGL GLVQPSYGQD GMYQRFLRQH VHPEETGGSD RYCNLMMQRR 

        70         80         90        100        110        120 
KMTLYHCKRF NTFIHEDIWN IRSICSTTNI QCKNGKMNCH EGVVKVTDCR DTGSSRAPNC 

       130        140 
RYRAIASTRR VVIACEGNPQ VPVHFDG

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and expression of human ribonuclease 4 cDNA." Seno M., Futami J., Tsushima Y., Akutagawa K., Kosaka M., Tada H., Yamada H. Biochim. Biophys. Acta 1261:424-426(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pancreas.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas.
[3]	"Human ribonuclease 4 (RNase 4): coding sequence, chromosomal localization and identification of two distinct transcripts in human somatic tissues." Rosenberg H.F., Dyer K.D. Nucleic Acids Res. 23:4290-4295(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 29-147.
[4]	"The amino acid sequence of human ribonuclease 4, a highly conserved ribonuclease that cleaves specifically on the 3' side of uridine." Zhou H.-M., Strydom D.J. Eur. J. Biochem. 217:401-410(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 29-147. Tissue: Plasma.
[5]	"Isolation and characterization of a human colon carcinoma-secreted enzyme with pancreatic ribonuclease-like activity." Shapiro R., Fett J.W., Strydom D.J., Vallee B.L. Biochemistry 25:7255-7264(1986) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[6]	"The three-dimensional structure of human RNase 4, unliganded and complexed with d(Up), reveals the basis for its uridine selectivity." Terzyan S.S., Peracaula R., de Llorens R., Tsushima Y., Yamada H., Seno M., Gomis-Rueth F.-X., Coll M. J. Mol. Biol. 285:205-214(1999) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 29-147.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D37931 mRNA. Translation: BAA07150.1.
BC015520 mRNA. Translation: AAH15520.1.
U36775 Genomic DNA. Translation: AAA96750.1.

IPI

IPI00029699.

PIR

I52489.

RefSeq

NP_002928.1. NM_002937.3.
NP_919412.1. NM_194431.1.

UniGene

Hs.730830.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1RNF	X-ray	2.10	A/B	29-147	[»]
2RNF	X-ray	2.40	A/B	29-147	[»]

ProteinModelPortal

P34096.

ModBase

Search...

Polymorphism databases

DMDM

1710614.

Proteomic databases

PaxDb

P34096.

PeptideAtlas

P34096.

PRIDE

P34096.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000304704; ENSP00000307096; ENSG00000181784.
ENST00000397999; ENSP00000381085; ENSG00000181784.
ENST00000398001; ENSP00000381087; ENSG00000181784.

GeneID

6038.

KEGG

hsa:6038.

UCSC

uc001vxy.4. human.

Organism-specific databases

CTD

6038.

GeneCards

GC14P021170.
GC14P021173.

HGNC

HGNC:10047. RNASE4.

HPA

CAB013673.

MIM

601030. gene.

neXtProt

NX_P34096.

PharmGKB

PA34415.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG42321.

HOGENOM

HOG000276883.

HOVERGEN

HBG008396.

InParanoid

P34096.

K16632.

OMA

SSRAPNC.

OrthoDB

EOG4TMR3F.

PhylomeDB

P34096.

Gene expression databases

ArrayExpress

P34096.

Bgee

P34096.

Genevestigator

P34096.

GermOnline

ENSG00000181784. Homo sapiens.

Family and domain databases

Gene3D

3.10.130.10. 1 hit.

InterPro

IPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]

PANTHER

PTHR11437. PTHR11437. 1 hit.

Pfam

PF00074. RnaseA. 1 hit.
[Graphical view]

PRINTS

PR00794. RIBONUCLEASE.

ProDom

PD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00092. RNAse_Pc. 1 hit.
[Graphical view]

SUPFAM

SSF54076. RNaseA. 1 hit.

PROSITE

PS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P34096.

GenomeRNAi

6038.

NextBio

23533.

SOURCE

Search...

Entry information

Entry name

RNAS4_HUMAN

Accession

Primary (citable) accession number: P34096

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 129 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Natural variations

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Polymorphism databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents