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P34096

- RNAS4_HUMAN

UniProt

P34096 - RNAS4_HUMAN

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Protein

Ribonuclease 4

Gene
RNASE4, RNS4
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

This RNase has marked specificity towards the 3' side of uridine nucleotides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381Substrate
Active sitei40 – 401Proton acceptor
Binding sitei93 – 931Substrate
Binding sitei110 – 1101Substrate
Active sitei144 – 1441Proton donor

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. pancreatic ribonuclease activity Source: UniProtKB

GO - Biological processi

  1. mRNA cleavage Source: UniProtKB
  2. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 4 (EC:3.1.27.-)
Short name:
RNase 4
Gene namesi
Name:RNASE4
Synonyms:RNS4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 14

Organism-specific databases

HGNCiHGNC:10047. RNASE4.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
  2. extracellular vesicular exosome Source: UniProt
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34415.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 28281 PublicationAdd
BLAST
Chaini29 – 147119Ribonuclease 4PRO_0000030883Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei29 – 291Pyrrolidone carboxylic acid
Disulfide bondi53 ↔ 1091 Publication
Disulfide bondi67 ↔ 1201 Publication
Disulfide bondi85 ↔ 1351 Publication
Disulfide bondi92 ↔ 991 Publication

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

MaxQBiP34096.
PaxDbiP34096.
PeptideAtlasiP34096.
PRIDEiP34096.

Expressioni

Gene expression databases

BgeeiP34096.
GenevestigatoriP34096.

Organism-specific databases

HPAiHPA048300.

Interactioni

Protein-protein interaction databases

BioGridi111967. 1 interaction.

Structurei

Secondary structure

1
147
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi29 – 4012
Helixi50 – 5910
Beta strandi62 – 665
Beta strandi69 – 746
Helixi78 – 825
Helixi83 – 864
Beta strandi99 – 11113
Beta strandi113 – 1153
Beta strandi122 – 13615
Turni137 – 1404
Beta strandi141 – 1477

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RNFX-ray2.10A/B29-147[»]
2RNFX-ray2.40A/B29-147[»]
ProteinModelPortaliP34096.
SMRiP34096. Positions 29-147.

Miscellaneous databases

EvolutionaryTraceiP34096.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni68 – 725Substrate binding

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG42321.
HOGENOMiHOG000276883.
HOVERGENiHBG008396.
InParanoidiP34096.
KOiK16632.
OMAiCHDEITE.
PhylomeDBiP34096.
TreeFamiTF333393.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P34096-1 [UniParc]FASTAAdd to Basket

« Hide

MALQRTHSLL LLLLLTLLGL GLVQPSYGQD GMYQRFLRQH VHPEETGGSD    50
RYCNLMMQRR KMTLYHCKRF NTFIHEDIWN IRSICSTTNI QCKNGKMNCH 100
EGVVKVTDCR DTGSSRAPNC RYRAIASTRR VVIACEGNPQ VPVHFDG 147
Length:147
Mass (Da):16,840
Last modified:October 1, 1996 - v3
Checksum:i4C24FEA249F3EC2F
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti16 – 161T → S.
Corresponds to variant rs3748338 [ dbSNP | Ensembl ].
VAR_024618

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti54 – 541N → D in AAA96750. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D37931 mRNA. Translation: BAA07150.1.
BC015520 mRNA. Translation: AAH15520.1.
U36775 Genomic DNA. Translation: AAA96750.1.
CCDSiCCDS9555.1.
PIRiI52489.
RefSeqiNP_001269121.1. NM_001282192.1.
NP_001269122.1. NM_001282193.1.
NP_002928.1. NM_002937.4.
NP_919412.1. NM_194431.2.
UniGeneiHs.283749.

Genome annotation databases

EnsembliENST00000304704; ENSP00000307096; ENSG00000258818.
ENST00000397995; ENSP00000381081; ENSG00000258818.
ENST00000555597; ENSP00000451624; ENSG00000258818.
ENST00000555835; ENSP00000452245; ENSG00000258818.
GeneIDi6038.
KEGGihsa:6038.
UCSCiuc001vxy.4. human.

Polymorphism databases

DMDMi1710614.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D37931 mRNA. Translation: BAA07150.1 .
BC015520 mRNA. Translation: AAH15520.1 .
U36775 Genomic DNA. Translation: AAA96750.1 .
CCDSi CCDS9555.1.
PIRi I52489.
RefSeqi NP_001269121.1. NM_001282192.1.
NP_001269122.1. NM_001282193.1.
NP_002928.1. NM_002937.4.
NP_919412.1. NM_194431.2.
UniGenei Hs.283749.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1RNF X-ray 2.10 A/B 29-147 [» ]
2RNF X-ray 2.40 A/B 29-147 [» ]
ProteinModelPortali P34096.
SMRi P34096. Positions 29-147.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 111967. 1 interaction.

Polymorphism databases

DMDMi 1710614.

Proteomic databases

MaxQBi P34096.
PaxDbi P34096.
PeptideAtlasi P34096.
PRIDEi P34096.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000304704 ; ENSP00000307096 ; ENSG00000258818 .
ENST00000397995 ; ENSP00000381081 ; ENSG00000258818 .
ENST00000555597 ; ENSP00000451624 ; ENSG00000258818 .
ENST00000555835 ; ENSP00000452245 ; ENSG00000258818 .
GeneIDi 6038.
KEGGi hsa:6038.
UCSCi uc001vxy.4. human.

Organism-specific databases

CTDi 6038.
GeneCardsi GC14P021185.
HGNCi HGNC:10047. RNASE4.
HPAi HPA048300.
MIMi 601030. gene.
neXtProti NX_P34096.
PharmGKBi PA34415.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG42321.
HOGENOMi HOG000276883.
HOVERGENi HBG008396.
InParanoidi P34096.
KOi K16632.
OMAi CHDEITE.
PhylomeDBi P34096.
TreeFami TF333393.

Miscellaneous databases

EvolutionaryTracei P34096.
GeneWikii Ribonuclease_4.
GenomeRNAii 6038.
NextBioi 23533.
PROi P34096.
SOURCEi Search...

Gene expression databases

Bgeei P34096.
Genevestigatori P34096.

Family and domain databases

Gene3Di 3.10.130.10. 1 hit.
InterProi IPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view ]
PANTHERi PTHR11437. PTHR11437. 1 hit.
Pfami PF00074. RnaseA. 1 hit.
[Graphical view ]
PRINTSi PR00794. RIBONUCLEASE.
ProDomi PD000535. RNaseA. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00092. RNAse_Pc. 1 hit.
[Graphical view ]
SUPFAMi SSF54076. SSF54076. 1 hit.
PROSITEi PS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pancreas.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pancreas.
  3. "Human ribonuclease 4 (RNase 4): coding sequence, chromosomal localization and identification of two distinct transcripts in human somatic tissues."
    Rosenberg H.F., Dyer K.D.
    Nucleic Acids Res. 23:4290-4295(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 29-147.
  4. "The amino acid sequence of human ribonuclease 4, a highly conserved ribonuclease that cleaves specifically on the 3' side of uridine."
    Zhou H.-M., Strydom D.J.
    Eur. J. Biochem. 217:401-410(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 29-147, PYROGLUTAMATE FORMATION AT GLN-29, DISULFIDE BONDS.
    Tissue: Plasma.
  5. "Isolation and characterization of a human colon carcinoma-secreted enzyme with pancreatic ribonuclease-like activity."
    Shapiro R., Fett J.W., Strydom D.J., Vallee B.L.
    Biochemistry 25:7255-7264(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "The three-dimensional structure of human RNase 4, unliganded and complexed with d(Up), reveals the basis for its uridine selectivity."
    Terzyan S.S., Peracaula R., de Llorens R., Tsushima Y., Yamada H., Seno M., Gomis-Rueth F.-X., Coll M.
    J. Mol. Biol. 285:205-214(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 29-147.

Entry informationi

Entry nameiRNAS4_HUMAN
AccessioniPrimary (citable) accession number: P34096
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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