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Protein

Ribonuclease 4

Gene

RNASE4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This RNase has marked specificity towards the 3' side of uridine nucleotides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38Substrate1
Active sitei40Proton acceptor1
Binding sitei93Substrate1
Binding sitei110Substrate1
Active sitei144Proton donor1

GO - Molecular functioni

  • endonuclease activity Source: GO_Central
  • nucleic acid binding Source: InterPro
  • ribonuclease A activity Source: UniProtKB
  • ribonuclease activity Source: GO_Central

GO - Biological processi

  • mRNA cleavage Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Enzyme and pathway databases

BioCyciZFISH:HS11659-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 4 (EC:3.1.27.-)
Short name:
RNase 4
Gene namesi
Name:RNASE4
Synonyms:RNS4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:10047. RNASE4.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi6038.
OpenTargetsiENSG00000258818.
PharmGKBiPA34415.

Polymorphism and mutation databases

BioMutaiRNASE4.
DMDMi1710614.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 281 PublicationAdd BLAST28
ChainiPRO_000003088329 – 147Ribonuclease 4Add BLAST119

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei29Pyrrolidone carboxylic acid1 Publication1
Disulfide bondi53 ↔ 1091 Publication
Disulfide bondi67 ↔ 1201 Publication
Disulfide bondi85 ↔ 1351 Publication
Disulfide bondi92 ↔ 991 Publication

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiP34096.
PeptideAtlasiP34096.
PRIDEiP34096.

Expressioni

Gene expression databases

BgeeiENSG00000258818.
ExpressionAtlasiP34096. baseline and differential.
GenevisibleiP34096. HS.

Interactioni

Protein-protein interaction databases

BioGridi111967. 2 interactors.
STRINGi9606.ENSP00000381081.

Structurei

Secondary structure

1147
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi29 – 40Combined sources12
Helixi50 – 59Combined sources10
Beta strandi62 – 66Combined sources5
Beta strandi69 – 74Combined sources6
Helixi78 – 82Combined sources5
Helixi83 – 86Combined sources4
Beta strandi99 – 111Combined sources13
Beta strandi113 – 115Combined sources3
Beta strandi122 – 136Combined sources15
Turni137 – 140Combined sources4
Beta strandi141 – 147Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RNFX-ray2.10A/B29-147[»]
2RNFX-ray2.40A/B29-147[»]
ProteinModelPortaliP34096.
SMRiP34096.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP34096.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni68 – 72Substrate binding5

Sequence similaritiesi

Belongs to the pancreatic ribonuclease family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IZBW. Eukaryota.
ENOG410Y4FD. LUCA.
GeneTreeiENSGT00840000129759.
HOGENOMiHOG000276883.
HOVERGENiHBG008396.
InParanoidiP34096.
KOiK16632.
OMAiSSRAPNC.
OrthoDBiEOG091G0YDD.
PhylomeDBiP34096.
TreeFamiTF333393.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P34096-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALQRTHSLL LLLLLTLLGL GLVQPSYGQD GMYQRFLRQH VHPEETGGSD
60 70 80 90 100
RYCNLMMQRR KMTLYHCKRF NTFIHEDIWN IRSICSTTNI QCKNGKMNCH
110 120 130 140
EGVVKVTDCR DTGSSRAPNC RYRAIASTRR VVIACEGNPQ VPVHFDG
Length:147
Mass (Da):16,840
Last modified:October 1, 1996 - v3
Checksum:i4C24FEA249F3EC2F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti54N → D in AAA96750 (PubMed:7501448).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02461816T → S.Corresponds to variant rs3748338dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37931 mRNA. Translation: BAA07150.1.
BC015520 mRNA. Translation: AAH15520.1.
U36775 Genomic DNA. Translation: AAA96750.1.
CCDSiCCDS9555.1.
PIRiI52489.
RefSeqiNP_001269121.1. NM_001282192.1.
NP_001269122.1. NM_001282193.1.
NP_002928.1. NM_002937.4.
NP_919412.1. NM_194431.2.
UniGeneiHs.283749.

Genome annotation databases

EnsembliENST00000397995; ENSP00000381081; ENSG00000258818.
ENST00000555597; ENSP00000451624; ENSG00000258818.
ENST00000555835; ENSP00000452245; ENSG00000258818.
GeneIDi6038.
KEGGihsa:6038.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37931 mRNA. Translation: BAA07150.1.
BC015520 mRNA. Translation: AAH15520.1.
U36775 Genomic DNA. Translation: AAA96750.1.
CCDSiCCDS9555.1.
PIRiI52489.
RefSeqiNP_001269121.1. NM_001282192.1.
NP_001269122.1. NM_001282193.1.
NP_002928.1. NM_002937.4.
NP_919412.1. NM_194431.2.
UniGeneiHs.283749.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RNFX-ray2.10A/B29-147[»]
2RNFX-ray2.40A/B29-147[»]
ProteinModelPortaliP34096.
SMRiP34096.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111967. 2 interactors.
STRINGi9606.ENSP00000381081.

Polymorphism and mutation databases

BioMutaiRNASE4.
DMDMi1710614.

Proteomic databases

PaxDbiP34096.
PeptideAtlasiP34096.
PRIDEiP34096.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000397995; ENSP00000381081; ENSG00000258818.
ENST00000555597; ENSP00000451624; ENSG00000258818.
ENST00000555835; ENSP00000452245; ENSG00000258818.
GeneIDi6038.
KEGGihsa:6038.

Organism-specific databases

CTDi6038.
DisGeNETi6038.
GeneCardsiRNASE4.
HGNCiHGNC:10047. RNASE4.
MIMi601030. gene.
neXtProtiNX_P34096.
OpenTargetsiENSG00000258818.
PharmGKBiPA34415.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IZBW. Eukaryota.
ENOG410Y4FD. LUCA.
GeneTreeiENSGT00840000129759.
HOGENOMiHOG000276883.
HOVERGENiHBG008396.
InParanoidiP34096.
KOiK16632.
OMAiSSRAPNC.
OrthoDBiEOG091G0YDD.
PhylomeDBiP34096.
TreeFamiTF333393.

Enzyme and pathway databases

BioCyciZFISH:HS11659-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP34096.
GeneWikiiRibonuclease_4.
GenomeRNAii6038.
PROiP34096.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000258818.
ExpressionAtlasiP34096. baseline and differential.
GenevisibleiP34096. HS.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRNAS4_HUMAN
AccessioniPrimary (citable) accession number: P34096
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 160 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.