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P34096 (RNAS4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 140. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease 4

Short name=RNase 4
EC=3.1.27.-
Gene names
Name:RNASE4
Synonyms:RNS4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length147 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This RNase has marked specificity towards the 3' side of uridine nucleotides.

Subcellular location

Secreted.

Sequence similarities

Belongs to the pancreatic ribonuclease family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Ref.4
Chain29 – 147119Ribonuclease 4
PRO_0000030883

Regions

Region68 – 725Substrate binding

Sites

Active site401Proton acceptor
Active site1441Proton donor
Binding site381Substrate
Binding site931Substrate
Binding site1101Substrate

Amino acid modifications

Modified residue291Pyrrolidone carboxylic acid
Disulfide bond53 ↔ 109 Ref.4
Disulfide bond67 ↔ 120 Ref.4
Disulfide bond85 ↔ 135 Ref.4
Disulfide bond92 ↔ 99 Ref.4

Natural variations

Natural variant161T → S.
Corresponds to variant rs3748338 [ dbSNP | Ensembl ].
VAR_024618

Experimental info

Sequence conflict541N → D in AAA96750. Ref.3

Secondary structure

................... 147
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P34096 [UniParc].

Last modified October 1, 1996. Version 3.
Checksum: 4C24FEA249F3EC2F

FASTA14716,840
        10         20         30         40         50         60 
MALQRTHSLL LLLLLTLLGL GLVQPSYGQD GMYQRFLRQH VHPEETGGSD RYCNLMMQRR 

        70         80         90        100        110        120 
KMTLYHCKRF NTFIHEDIWN IRSICSTTNI QCKNGKMNCH EGVVKVTDCR DTGSSRAPNC 

       130        140 
RYRAIASTRR VVIACEGNPQ VPVHFDG 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and expression of human ribonuclease 4 cDNA."
Seno M., Futami J., Tsushima Y., Akutagawa K., Kosaka M., Tada H., Yamada H.
Biochim. Biophys. Acta 1261:424-426(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Pancreas.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Pancreas.
[3]"Human ribonuclease 4 (RNase 4): coding sequence, chromosomal localization and identification of two distinct transcripts in human somatic tissues."
Rosenberg H.F., Dyer K.D.
Nucleic Acids Res. 23:4290-4295(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 29-147.
[4]"The amino acid sequence of human ribonuclease 4, a highly conserved ribonuclease that cleaves specifically on the 3' side of uridine."
Zhou H.-M., Strydom D.J.
Eur. J. Biochem. 217:401-410(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 29-147, PYROGLUTAMATE FORMATION AT GLN-29, DISULFIDE BONDS.
Tissue: Plasma.
[5]"Isolation and characterization of a human colon carcinoma-secreted enzyme with pancreatic ribonuclease-like activity."
Shapiro R., Fett J.W., Strydom D.J., Vallee B.L.
Biochemistry 25:7255-7264(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"The three-dimensional structure of human RNase 4, unliganded and complexed with d(Up), reveals the basis for its uridine selectivity."
Terzyan S.S., Peracaula R., de Llorens R., Tsushima Y., Yamada H., Seno M., Gomis-Rueth F.-X., Coll M.
J. Mol. Biol. 285:205-214(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 29-147.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D37931 mRNA. Translation: BAA07150.1.
BC015520 mRNA. Translation: AAH15520.1.
U36775 Genomic DNA. Translation: AAA96750.1.
CCDSCCDS9555.1.
PIRI52489.
RefSeqNP_001269121.1. NM_001282192.1.
NP_001269122.1. NM_001282193.1.
NP_002928.1. NM_002937.4.
NP_919412.1. NM_194431.2.
UniGeneHs.283749.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1RNFX-ray2.10A/B29-147[»]
2RNFX-ray2.40A/B29-147[»]
ProteinModelPortalP34096.
SMRP34096. Positions 29-147.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111967. 1 interaction.

Polymorphism databases

DMDM1710614.

Proteomic databases

MaxQBP34096.
PaxDbP34096.
PeptideAtlasP34096.
PRIDEP34096.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000304704; ENSP00000307096; ENSG00000258818.
ENST00000397995; ENSP00000381081; ENSG00000258818.
ENST00000555597; ENSP00000451624; ENSG00000258818.
ENST00000555835; ENSP00000452245; ENSG00000258818.
GeneID6038.
KEGGhsa:6038.
UCSCuc001vxy.4. human.

Organism-specific databases

CTD6038.
GeneCardsGC14P021185.
HGNCHGNC:10047. RNASE4.
HPAHPA048300.
MIM601030. gene.
neXtProtNX_P34096.
PharmGKBPA34415.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG42321.
HOGENOMHOG000276883.
HOVERGENHBG008396.
InParanoidP34096.
KOK16632.
OMACHDEITE.
PhylomeDBP34096.
TreeFamTF333393.

Gene expression databases

BgeeP34096.
GenevestigatorP34096.

Family and domain databases

Gene3D3.10.130.10. 1 hit.
InterProIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERPTHR11437. PTHR11437. 1 hit.
PfamPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSPR00794. RIBONUCLEASE.
ProDomPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMSSF54076. SSF54076. 1 hit.
PROSITEPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP34096.
GeneWikiRibonuclease_4.
GenomeRNAi6038.
NextBio23533.
PROP34096.
SOURCESearch...

Entry information

Entry nameRNAS4_HUMAN
AccessionPrimary (citable) accession number: P34096
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 140 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM