Reviewed, UniProtKB/Swiss-Prot P34093 (NDKM_DICDI)

Names and origin

Protein names

Recommended name:
    Nucleoside diphosphate kinase, mitochondrial
      Short name=NDP kinase
      Short name=NDK
    EC=2.7.4.6

Gene names

Name:	ndkM
Synonyms:	guk, ndkA
ORF Names:	DDB_G0279911

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.
Cofactor	Magnesium By similarity.
Subcellular location	Mitochondrion intermembrane space.
Sequence similarities	Belongs to the NDK family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	CTP biosynthetic process Inferred from electronic annotation. Source: InterPro GTP biosynthetic process Inferred from electronic annotation. Source: InterPro UTP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial intermembrane space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 57	57	Mitochondrion Ref.1
	Chain	58 – 220	163	Nucleoside diphosphate kinase, mitochondrial		PRO_0000019430
Sites
	Active site	186	1	Pros-phosphohistidine intermediate By similarity
	Binding site	80	1	ATP By similarity
	Binding site	128	1	ATP By similarity
	Binding site	156	1	ATP By similarity
	Binding site	162	1	ATP By similarity
	Binding site	173	1	ATP By similarity
	Binding site	183	1	ATP By similarity
Experimental info
	Sequence conflict	30	1	A → G in AAA16162. Ref.1
	Sequence conflict	81 – 82	2	VS → WN in AAA16162. Ref.1
	Sequence conflict	163	1	D → V in AAA16162. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P34093-1 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: 42A82CA730CF0FDA	FASTA	220	23,998
	10 20 30 40 50 60 MFSRFARAFP KILASGASQR TFATVQKAFA NPTSKKLIVG SSLLIGSAFA TTSFVACENK 70 80 90 100 110 120 SVPLVGLPGT NQERSFIAIK VSSTQRRLIG EIIARFEKKG FKLVGIKILV PTPEHAAKHY 130 140 150 160 170 180 EDLNKKPFFN GLVKFFSSGA VVAMVFEGKD VVRTGRVLIG ATDPSQSAPG TIRFDLCIET 190 200 210 220 GRNIIHGSDS NESAAHEIAL WFKEDEIANW VSTNPVYEKM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Separate nuclear genes encode cytosolic and mitochondrial nucleoside diphosphate kinase in Dictyostelium discoideum." Troll H., Winckler T., Lascu I., Mueller N., Saurin W., Veron M., Mutzel R. J. Biol. Chem. 268:25469-25475(1993) [PubMed: 8244981] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 58-64. Strain: AX2.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L23068 Genomic DNA. Translation: AAA16162.1. AAFI02000035 Genomic DNA. Translation: EAL67427.1.
PIR	B49547.
RefSeq	XP_641417.1.
3D structure databases
HSSP	HSSP built from PDB template 1NPK based on UniProtKB P22887.
ModBase	Search...
Protein-protein interaction databases
STRING	P34093.
Genome annotation databases
GeneID	3392555.
GenomeReviews	Gene locus ndkM in contig CM000152_GR.
KEGG	ddi:DDB_0214817.
Organism-specific databases
dictyBase	DDB_G0279911. ndkM.
Phylogenomic databases
OMA	KEDEIAN.
Enzyme and pathway databases
BRENDA	2.7.4.6. 424.
Family and domain databases
InterPro	IPR001564. Nuc_diP_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.70.141. NDK. 1 hit.
PANTHER	PTHR11349. Nuc_diP_kinase_core. 1 hit.
Pfam	PF00334. NDK. 1 hit. [Graphical view]
PRINTS	PR01243. NUCDPKINASE.
ProDom	PD001018. NDK. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00562. NDK. 1 hit. [Graphical view]
PROSITE	PS00469. NDP_KINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDKM_DICDI

Accession

Primary (citable) accession number: P34093
Secondary accession number(s): Q54W33

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	December 4, 2007
Last modified:	November 3, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)