Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P34085 (CISY_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Citrate synthase, mitochondrial

EC=2.3.3.1
Gene names
Name:cit-1
ORF Names:B1D1.330, NCU01692
OrganismNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]
Taxonomic identifier367110 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora

Protein attributes

Sequence length469 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.

Subcellular location

Mitochondrion matrix.

Developmental stage

Abundant after 6-12 hours of growth. It is not significantly expressed after 24 hours, which is several hours after entering the stationary phase of growth.

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Sequence similarities

Belongs to the citrate synthase family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   Cellular componentMitochondrion
   DomainTransit peptide
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processacetyl-CoA catabolic process

Inferred from electronic annotation. Source: EnsemblFungi

cellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentmitochondrial matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

peroxisome

Inferred from electronic annotation. Source: EnsemblFungi

   Molecular_functioncitrate (Si)-synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3333Mitochondrion Potential
Chain34 – 469436Citrate synthase, mitochondrial
PRO_0000005480

Sites

Active site3521 By similarity

Experimental info

Sequence conflict3511G → A in AAA16630. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P34085 [UniParc].

Last modified April 30, 2003. Version 2.
Checksum: C14FFDDE07D345FD

FASTA46951,989
        10         20         30         40         50         60 
MAPVMRLGSA ALRSSIHLTS RQTAFTAARC YSSKTQTLKE RFAELLPENI EKIKALRKEH 

        70         80         90        100        110        120 
GSKVVDKVTL DQVYGGARGI KCLVWEGSVL DAEEGIRFRG KTIPECQELL PKAPGGKEPL 

       130        140        150        160        170        180 
PEGLFWLLLT GEVPSEQQVR DLSAEWAARS DVPKFIEELI DRCPSDLHPM AQLSLAVTAL 

       190        200        210        220        230        240 
EHTSSFARAY AKGINKKEYW GYTFEDSMDL IAKLPTIAAR IYQNVFKGGK VAAVQKDKDY 

       250        260        270        280        290        300 
SFNFANQLGF GDNKDFVELL RLYLTIHTDH EGGNVSAHTT HLVGSALSSP FLSVAAGLNG 

       310        320        330        340        350        360 
LAGPLHGLAN QEVLNWLTEM KKVIGDDLSD EAITKYLWDT LNAGRVVPGY GHAVLRKTDP 

       370        380        390        400        410        420 
RYSAQRKFAQ EHLPEDPMFQ LVSQVYKIAP KVLTEHGKTK NPYPNVDAHS GVLLQHYGLT 

       430        440        450        460 
EANYYTVLFG VSRAIGVLPQ LIIDRAVGAP IERPKSYSTD KWIEICKKL 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of the cit-1 gene from Neurospora crassa encoding the mitochondrial form of citrate synthase."
Ferea T., Contreras E.T., Oung T., Bowman E.J., Bowman B.J.
Mol. Gen. Genet. 242:105-110(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: 74A.
[2]"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]"The genome sequence of the filamentous fungus Neurospora crassa."
Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. expand/collapse author list , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M84187 Unassigned DNA. Translation: AAA16630.1.
AL355927 Genomic DNA. Translation: CAB91282.1.
CM002237 Genomic DNA. Translation: EAA27662.1.
PIRS41563.
RefSeqXP_956898.1. XM_951805.2.
UniGeneNcr.20011.

3D structure databases

ProteinModelPortalP34085.
SMRP34085. Positions 33-465.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5141.NCU01692.1.

Proteomic databases

PRIDEP34085.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiEFNCRT00000001887; EFNCRP00000001887; EFNCRG00000001885.
GeneID3873020.
KEGGncr:NCU01692.

Phylogenomic databases

eggNOGCOG0372.
HOGENOMHOG000130831.
KOK01647.
OrthoDBEOG7WQ82G.

Enzyme and pathway databases

UniPathwayUPA00223; UER00717.

Family and domain databases

Gene3D1.10.580.10. 1 hit.
InterProIPR016142. Citrate_synth-like_lrg_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR010109. Citrate_synthase_euk.
[Graphical view]
PANTHERPTHR11739. PTHR11739. 1 hit.
PfamPF00285. Citrate_synt. 1 hit.
[Graphical view]
PRINTSPR00143. CITRTSNTHASE.
SUPFAMSSF48256. SSF48256. 1 hit.
TIGRFAMsTIGR01793. cit_synth_euk. 1 hit.
PROSITEPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCISY_NEUCR
AccessionPrimary (citable) accession number: P34085
Secondary accession number(s): Q7RY26, Q9P688
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: April 30, 2003
Last modified: February 19, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways