SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P34085

- CISY_NEUCR

UniProt

P34085 - CISY_NEUCR

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Citrate synthase, mitochondrial
Gene
cit-1, B1D1.330, NCU01692
Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei352 – 3521 By similarity

GO - Molecular functioni

  1. citrate (Si)-synthase activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. acetyl-CoA catabolic process Source: EnsemblFungi
  2. cellular carbohydrate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00223; UER00717.

Names & Taxonomyi

Protein namesi
Recommended name:
Citrate synthase, mitochondrial (EC:2.3.3.16)
Gene namesi
Name:cit-1
ORF Names:B1D1.330, NCU01692
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805: Chromosome 6, Linkage Group II

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
  2. peroxisome Source: EnsemblFungi
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3333Mitochondrion Reviewed prediction
Add
BLAST
Chaini34 – 469436Citrate synthase, mitochondrial
PRO_0000005480Add
BLAST

Proteomic databases

PRIDEiP34085.

Expressioni

Developmental stagei

Abundant after 6-12 hours of growth. It is not significantly expressed after 24 hours, which is several hours after entering the stationary phase of growth.

Interactioni

Protein-protein interaction databases

STRINGi5141.NCU01692.1.

Structurei

3D structure databases

ProteinModelPortaliP34085.
SMRiP34085. Positions 33-465.

Family & Domainsi

Sequence similaritiesi

Belongs to the citrate synthase family.

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0372.
HOGENOMiHOG000130831.
KOiK01647.
OrthoDBiEOG7WQ82G.

Family and domain databases

Gene3Di1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR010109. Citrate_synthase_euk.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01793. cit_synth_euk. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P34085-1 [UniParc]FASTAAdd to Basket

« Hide

MAPVMRLGSA ALRSSIHLTS RQTAFTAARC YSSKTQTLKE RFAELLPENI    50
EKIKALRKEH GSKVVDKVTL DQVYGGARGI KCLVWEGSVL DAEEGIRFRG 100
KTIPECQELL PKAPGGKEPL PEGLFWLLLT GEVPSEQQVR DLSAEWAARS 150
DVPKFIEELI DRCPSDLHPM AQLSLAVTAL EHTSSFARAY AKGINKKEYW 200
GYTFEDSMDL IAKLPTIAAR IYQNVFKGGK VAAVQKDKDY SFNFANQLGF 250
GDNKDFVELL RLYLTIHTDH EGGNVSAHTT HLVGSALSSP FLSVAAGLNG 300
LAGPLHGLAN QEVLNWLTEM KKVIGDDLSD EAITKYLWDT LNAGRVVPGY 350
GHAVLRKTDP RYSAQRKFAQ EHLPEDPMFQ LVSQVYKIAP KVLTEHGKTK 400
NPYPNVDAHS GVLLQHYGLT EANYYTVLFG VSRAIGVLPQ LIIDRAVGAP 450
IERPKSYSTD KWIEICKKL 469
Length:469
Mass (Da):51,989
Last modified:April 30, 2003 - v2
Checksum:iC14FFDDE07D345FD
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti351 – 3511G → A in AAA16630. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M84187 Unassigned DNA. Translation: AAA16630.1.
AL355927 Genomic DNA. Translation: CAB91282.1.
CM002237 Genomic DNA. Translation: EAA27662.1.
PIRiS41563.
RefSeqiXP_956898.1. XM_951805.2.
UniGeneiNcr.20011.

Genome annotation databases

EnsemblFungiiEFNCRT00000001887; EFNCRP00000001887; EFNCRG00000001885.
GeneIDi3873020.
KEGGincr:NCU01692.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M84187 Unassigned DNA. Translation: AAA16630.1 .
AL355927 Genomic DNA. Translation: CAB91282.1 .
CM002237 Genomic DNA. Translation: EAA27662.1 .
PIRi S41563.
RefSeqi XP_956898.1. XM_951805.2.
UniGenei Ncr.20011.

3D structure databases

ProteinModelPortali P34085.
SMRi P34085. Positions 33-465.
ModBasei Search...

Protein-protein interaction databases

STRINGi 5141.NCU01692.1.

Proteomic databases

PRIDEi P34085.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii EFNCRT00000001887 ; EFNCRP00000001887 ; EFNCRG00000001885 .
GeneIDi 3873020.
KEGGi ncr:NCU01692.

Phylogenomic databases

eggNOGi COG0372.
HOGENOMi HOG000130831.
KOi K01647.
OrthoDBi EOG7WQ82G.

Enzyme and pathway databases

UniPathwayi UPA00223 ; UER00717 .

Family and domain databases

Gene3Di 1.10.580.10. 1 hit.
InterProi IPR016142. Citrate_synth-like_lrg_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR010109. Citrate_synthase_euk.
[Graphical view ]
PANTHERi PTHR11739. PTHR11739. 1 hit.
Pfami PF00285. Citrate_synt. 1 hit.
[Graphical view ]
PRINTSi PR00143. CITRTSNTHASE.
SUPFAMi SSF48256. SSF48256. 1 hit.
TIGRFAMsi TIGR01793. cit_synth_euk. 1 hit.
PROSITEi PS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the cit-1 gene from Neurospora crassa encoding the mitochondrial form of citrate synthase."
    Ferea T., Contreras E.T., Oung T., Bowman E.J., Bowman B.J.
    Mol. Gen. Genet. 242:105-110(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 74A.
  2. "What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
    Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
    Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  3. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Entry informationi

Entry nameiCISY_NEUCR
AccessioniPrimary (citable) accession number: P34085
Secondary accession number(s): Q7RY26, Q9P688
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: April 30, 2003
Last modified: June 11, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi