P34081 (HMUI_BPSP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: DNA endonuclease I-HmuI EC=3.1.-.- Alternative name(s): HNH homing endonuclease I-HmuI
Organism	Bacillus phage SP01 (Bacteriophage SP01)
Taxonomic identifier	10685 [NCBI]
Taxonomic lineage	Viruses › dsDNA viruses, no RNA stage › Caudovirales › Myoviridae › SPO1-like viruses
Virus host	Bacillus subtilis [TaxID: 1423]

Protein attributes

Sequence length	174 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Biological process	Intron homing
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	3D-structure
Gene Ontology (GO)
Biological process	intron homing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 174

174

DNA endonuclease I-HmuI

PRO_0000106168

Secondary structure

1

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174

Helix Strand Turn

Details...

Beta strand

3 – 5

3

Turn

10 – 12

3

Beta strand

13 – 16

4

Beta strand

21 – 26

6

Beta strand

28 – 30

3

Beta strand

41 – 46

6

Beta strand

49 – 54

6

Helix

55 – 63

9

Beta strand

72 – 75

4

Helix

85 – 87

3

Beta strand

88 – 91

4

Helix

93 – 103

11

Helix

109 – 118

10

Beta strand

123 – 126

4

Beta strand

132 – 136

5

Helix

138 – 145

8

Helix

149 – 156

8

Beta strand

159 – 161

3

Beta strand

166 – 170

5

Sequences

	Sequence		Length	Mass (Da)
	P34081 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: BBA60B1E00D8AE88	FASTA	174	19,766
	10 20 30 40 50 60 MEWKDIKGYE GHYQVSNTGE VYSIKSGKTL KHQIPKDGYH RIGLFKGGKG KTFQVHRLVA 70 80 90 100 110 120 IHFCEGYEEG LVVDHKDGNK DNNLSTNLRW VTQKINVENQ MSRGTLNVSK AQQIAKIKNQ 130 140 150 160 170 KPIIVISPDG IEKEYPSTKC ACEELGLTRG KVTDVLKGHR IHHKGYTFRY KLNG

References

[1]	"A self-splicing group I intron in the DNA polymerase gene of Bacillus subtilis bacteriophage SPO1." Goodrich-Blair H., Scarlato V., Gott J.M., Xu M.Q., Shub D.A. Cell 63:417-424(1990) [PubMed: 2119891] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"DNA binding and cleavage by the HNH homing endonuclease I-HmuI." Shen B.W., Landthaler M., Shub D.A., Stoddard B.L. J. Mol. Biol. 342:43-56(2004) [PubMed: 15313606] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.92 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M37686 Genomic DNA. Translation: AAA64536.1.

PIR

A36077.

RefSeq

YP_002300418.1. NC_011421.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1U3E	X-ray	2.92	M	1-174	[»]

ProteinModelPortal

P34081.

SMR

P34081. Positions 1-174.

ModBase

Search...

Protein family/group databases

REBASE

2628. I-HmuI.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

7009136.

Family and domain databases

InterPro

IPR003615. HNH_nuc.
IPR003647. Intron_nuc_1.
IPR010902. NUMOD4.
[Graphical view]

Pfam

PF07463. NUMOD4. 1 hit.
[Graphical view]

SMART

SM00507. HNHc. 1 hit.
SM00497. IENR1. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

HMUI_BPSP1

Accession

Primary (citable) accession number: P34081

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program