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Protein

DNA endonuclease I-HmuI

Gene
N/A
Organism
Bacillus phage SP01 (Bacteriophage SP01)
Status
Reviewed-Annotation score: Annotation score: 1 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Intron homing

Protein family/group databases

REBASEi2628. I-HmuI.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA endonuclease I-HmuI (EC:3.1.-.-)
Alternative name(s):
HNH homing endonuclease I-HmuI
OrganismiBacillus phage SP01 (Bacteriophage SP01)
Taxonomic identifieri10685 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeSpounavirinaeSpounalikevirus
Virus hostiBacillus subtilis [TaxID: 1423]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 174174DNA endonuclease I-HmuIPRO_0000106168Add
BLAST

Structurei

Secondary structure

1
174
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 53Combined sources
Turni10 – 123Combined sources
Beta strandi13 – 164Combined sources
Beta strandi21 – 266Combined sources
Beta strandi28 – 303Combined sources
Beta strandi41 – 466Combined sources
Beta strandi49 – 546Combined sources
Helixi55 – 639Combined sources
Beta strandi72 – 754Combined sources
Helixi85 – 873Combined sources
Beta strandi88 – 914Combined sources
Helixi93 – 10311Combined sources
Helixi109 – 11810Combined sources
Beta strandi123 – 1264Combined sources
Beta strandi132 – 1365Combined sources
Helixi138 – 1458Combined sources
Helixi149 – 1568Combined sources
Beta strandi159 – 1613Combined sources
Beta strandi166 – 1705Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1U3EX-ray2.92M1-174[»]
ProteinModelPortaliP34081.
SMRiP34081. Positions 1-174.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP34081.

Family & Domainsi

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR003615. HNH_nuc.
IPR003647. Intron_nuc_1_rpt.
IPR010902. NUMOD4.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF13392. HNH_3. 1 hit.
PF07463. NUMOD4. 1 hit.
[Graphical view]
SMARTiSM00507. HNHc. 1 hit.
SM00497. IENR1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34081-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEWKDIKGYE GHYQVSNTGE VYSIKSGKTL KHQIPKDGYH RIGLFKGGKG
60 70 80 90 100
KTFQVHRLVA IHFCEGYEEG LVVDHKDGNK DNNLSTNLRW VTQKINVENQ
110 120 130 140 150
MSRGTLNVSK AQQIAKIKNQ KPIIVISPDG IEKEYPSTKC ACEELGLTRG
160 170
KVTDVLKGHR IHHKGYTFRY KLNG
Length:174
Mass (Da):19,766
Last modified:February 1, 1994 - v1
Checksum:iBBA60B1E00D8AE88
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M37686 Genomic DNA. Translation: AAA64536.1.
PIRiA36077.
RefSeqiYP_002300418.1. NC_011421.1.

Genome annotation databases

GeneIDi7009136.
KEGGivg:7009136.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M37686 Genomic DNA. Translation: AAA64536.1.
PIRiA36077.
RefSeqiYP_002300418.1. NC_011421.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1U3EX-ray2.92M1-174[»]
ProteinModelPortaliP34081.
SMRiP34081. Positions 1-174.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi2628. I-HmuI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi7009136.
KEGGivg:7009136.

Miscellaneous databases

EvolutionaryTraceiP34081.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR003615. HNH_nuc.
IPR003647. Intron_nuc_1_rpt.
IPR010902. NUMOD4.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF13392. HNH_3. 1 hit.
PF07463. NUMOD4. 1 hit.
[Graphical view]
SMARTiSM00507. HNHc. 1 hit.
SM00497. IENR1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A self-splicing group I intron in the DNA polymerase gene of Bacillus subtilis bacteriophage SPO1."
    Goodrich-Blair H., Scarlato V., Gott J.M., Xu M.Q., Shub D.A.
    Cell 63:417-424(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "DNA binding and cleavage by the HNH homing endonuclease I-HmuI."
    Shen B.W., Landthaler M., Shub D.A., Stoddard B.L.
    J. Mol. Biol. 342:43-56(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.92 ANGSTROMS).

Entry informationi

Entry nameiHMUI_BPSP1
AccessioniPrimary (citable) accession number: P34081
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: December 9, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.