Reviewed, UniProtKB/Swiss-Prot P34080 (AQP2_RAT)

Names and originHide

Protein names

Recommended name:
Aquaporin-2
Short name=AQP-2

Alternative name(s):
Aquaporin-CD
Short name=AQP-CD
Water channel protein for renal collecting duct
ADH water channel
Collecting duct water channel protein
WCH-CD

Gene names

Name:

Aqp2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributesHide

Sequence length	271 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)Hide

Function	Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Subcellular location	Apical cell membrane; Multi-pass membrane protein By similarity. Cytoplasmic vesicle membrane; Multi-pass membrane protein By similarity. Note: Shuttles from vesicles to the apical membrane By similarity.
Tissue specificity	Expressed in renal collecting tubules.
Domain	Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Post-translational modification	Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent By similarity.
Sequence similarities	Belongs to the MIP/aquaporin (TC 1.A.8) family. [View classification]
Sequence caution	The sequence AAA41478.1 differs from that shown. Reason: Erroneous initiation.

OntologiesHide

Keywords
Biological process	Transport
Cellular component	Cell membrane Cytoplasmic vesicle Membrane
Domain	Repeat Transmembrane
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	actin filament depolymerization Inferred from direct assay. Source: RGD aging Inferred from expression pattern. Source: RGD apoptosis Inferred from mutant phenotype. Source: RGD cell volume homeostasis Inferred from mutant phenotype. Source: RGD cellular response to water deprivation Inferred from direct assay. Source: RGD female pregnancy Inferred from expression pattern. Source: RGD hyperosmotic response Inferred from expression pattern. Source: RGD positive regulation of calcium ion transport Inferred from mutant phenotype. Source: RGD response to calcium ion Inferred from expression pattern. Source: RGD response to glucagon stimulus Inferred from expression pattern. Source: RGD response to lipopolysaccharide Inferred from expression pattern. Source: RGD response to lithium ion Inferred from expression pattern. Source: RGD response to salt stress Inferred from expression pattern. Source: RGD response to starvation Inferred from expression pattern. Source: RGD transmembrane transport Inferred from electronic annotation. Source: InterPro water homeostasis Traceable author statement. Source: RGD
Cellular component	apical plasma membrane Inferred from direct assay. Source: UniProtKB basolateral plasma membrane Inferred from direct assay. Source: RGD clathrin-coated vesicle Inferred from direct assay. Source: RGD cytoplasmic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell early endosome Inferred from direct assay. Source: RGD exocytic vesicle Inferred from direct assay. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW lysosome Inferred from direct assay. Source: RGD protein complex Inferred from direct assay. Source: RGD rough endoplasmic reticulum Inferred from direct assay. Source: RGD trans-Golgi network Inferred from direct assay. Source: RGD
Molecular function	PDZ domain binding Inferred from physical interaction. Source: RGD actin binding Inferred from direct assay. Source: RGD water channel activity Traceable author statement. Source: RGD
Complete GO annotation...

Sequence annotation (Features)Hide

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	Aquaporin-2		PRO_0000063940
Regions
	Topological domain	1 – 16	16	Cytoplasmic Potential
	Transmembrane	17 – 34	18	Helical; Potential
	Topological domain	35 – 40	6	Extracellular Potential
	Transmembrane	41 – 59	19	Helical; Potential
	Topological domain	60 – 85	26	Cytoplasmic Potential
	Transmembrane	86 – 107	22	Helical; Potential
	Topological domain	108 – 127	20	Extracellular Potential
	Transmembrane	128 – 148	21	Helical; Potential
	Topological domain	149 – 156	8	Cytoplasmic Potential
	Transmembrane	157 – 176	20	Helical; Potential
	Topological domain	177 – 202	26	Extracellular Potential
	Transmembrane	203 – 224	22	Helical; Potential
	Topological domain	225 – 271	47	Cytoplasmic Potential
	Motif	68 – 70	3	NPA 1
	Motif	184 – 186	3	NPA 2
Amino acid modifications
	Modified residue	256	1	Phosphoserine Ref.5
	Modified residue	261	1	Phosphoserine Ref.5
	Modified residue	264	1	Phosphoserine Ref.5
	Modified residue	269	1	Phosphoserine Ref.5
	Glycosylation	124	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	54	1	I → T in AAI28706. Ref.4

SequencesHide

	Sequence		Length	Mass (Da)
	P34080-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 86722ED2BCE0B3E4	FASTA	271	28,931
	10 20 30 40 50 60 MWELRSIAFS RAVLAEFLAT LLFVFFGLGS ALQWASSPPS VLQIAVAFGL GIGILVQALG 70 80 90 100 110 120 HVSGAHINPA VTVACLVGCH VSFLRAAFYV AAQLLGAVAG AAILHEITPV EIRGDLAVNA 130 140 150 160 170 180 LHNNATAGQA VTVELFLTMQ LVLCIFASTD ERRGDNLGSP ALSIGFSVTL GHLLGIYFTG 190 200 210 220 230 240 CSMNPARSLA PAVVTGKFDD HWVFWIGPLV GAIIGSLLYN YLLFPSAKSL QERLAVLKGL 250 260 270 EPDTDWEERE VRRRQSVELH SPQSLPRGSK A

ReferencesHide

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of apical membrane water channel of rat kidney collecting tubule." Fushimi K., Uchida S., Hara Y., Hirata Y., Marumo F., Sasaki S. Nature 361:549-552(1993) [PubMed: 8429910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
[2]	"Cloning of a novel rat kidney cDNA homologous to CHIP28 and WCH-CD water channels." Ma T., Frigeri A., Skach W., Verkman A.S. Biochem. Biophys. Res. Commun. 197:654-659(1993) [PubMed: 7505572] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
[3]	"Expression, functional analysis, and in situ hybridization of a cloned rat kidney collecting duct water channel." Ma T., Hasegawa H., Skach W., Frigeri A., Verkman A.S. Am. J. Physiol. 266:C189-C197(1994) [PubMed: 7508187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[5]	"Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites." Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A. Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed: 16641100] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-256; SER-261; SER-264 AND SER-269, MASS SPECTROMETRY. Tissue: Renal collecting duct.
+	Additional computationally mapped references.

Cross-referencesHide

Sequence databases
EMBL GenBank DDBJ	D13906 mRNA. Translation: BAA03006.1. L28112 mRNA. Translation: AAA41478.1. Different initiation. BC128705 mRNA. Translation: AAI28706.1.
IPI	IPI00562696.
PIR	JT0750.
RefSeq	NP_037041.2.
UniGene	Rn.90076
3D structure databases
SMR	P34080. Positions 2-251.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-46223N.
STRING	P34080.
PTM databases
PhosphoSite	P34080.
Genome annotation databases
Ensembl	ENSRNOT00000000324; ENSRNOP00000000324; ENSRNOG00000000297; Rattus norvegicus. [Genome view]
GeneID	25386.
KEGG	rno:25386.
UCSC	NM_012909. rat.
Organism-specific databases
CTD	25386.
RGD	2142. Aqp2.
Phylogenomic databases
eggNOG	maNOG04117.
HOVERGEN	HBG000312.
InParanoid	P34080.
PhylomeDB	P34080.
Gene expression databases
ArrayExpress	P34080.
Genevestigator	P34080.
GermOnline	ENSRNOG00000000297. Rattus norvegicus.
Family and domain databases
InterPro	IPR012269. Aquaporin. IPR000425. MIP. IPR022357. MIP_CS. [Graphical view]
Gene3D	G3DSA:1.20.1080.10. MIP. 1 hit.
PANTHER	PTHR19139. MIP. 1 hit.
Pfam	PF00230. MIP. 1 hit. [Graphical view]
PRINTS	PR00783. MINTRINSICP.
SUPFAM	SSF81338. MIP. 1 hit.
TIGRFAMs	TIGR00861. MIP. 1 hit.
PROSITE	PS00221. MIP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	606439.

Entry informationHide

Entry name

AQP2_RAT

Accession

Primary (citable) accession number: P34080
Secondary accession number(s): A1A5L4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	July 13, 2010
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)