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Protein

Aquaporin-2

Gene

Aqp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

GO - Molecular functioni

  • actin binding Source: RGD
  • glycerol channel activity Source: GO_Central
  • PDZ domain binding Source: RGD
  • water channel activity Source: RGD
  • water transmembrane transporter activity Source: UniProtKB

GO - Biological processi

  • actin filament depolymerization Source: RGD
  • aging Source: RGD
  • apoptotic process Source: RGD
  • cellular response to water deprivation Source: RGD
  • cellular water homeostasis Source: GO_Central
  • cell volume homeostasis Source: RGD
  • female pregnancy Source: RGD
  • hyperosmotic response Source: RGD
  • ion transmembrane transport Source: GO_Central
  • positive regulation of calcium ion transport Source: RGD
  • response to calcium ion Source: RGD
  • response to glucagon Source: RGD
  • response to hormone Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to lithium ion Source: RGD
  • response to salt stress Source: RGD
  • response to starvation Source: RGD
  • response to water deprivation Source: RGD
  • water homeostasis Source: RGD
  • water transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Aquaporin-2
Short name:
AQP-2
Alternative name(s):
ADH water channel
Aquaporin-CD
Short name:
AQP-CD
Collecting duct water channel protein
WCH-CD
Water channel protein for renal collecting duct
Gene namesi
Name:Aqp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2142. Aqp2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1616CytoplasmicSequence analysisAdd
BLAST
Transmembranei17 – 3418HelicalSequence analysisAdd
BLAST
Topological domaini35 – 406ExtracellularSequence analysis
Transmembranei41 – 5919HelicalSequence analysisAdd
BLAST
Topological domaini60 – 8526CytoplasmicSequence analysisAdd
BLAST
Transmembranei86 – 10722HelicalSequence analysisAdd
BLAST
Topological domaini108 – 12720ExtracellularSequence analysisAdd
BLAST
Transmembranei128 – 14821HelicalSequence analysisAdd
BLAST
Topological domaini149 – 1568CytoplasmicSequence analysis
Transmembranei157 – 17620HelicalSequence analysisAdd
BLAST
Topological domaini177 – 20226ExtracellularSequence analysisAdd
BLAST
Transmembranei203 – 22422HelicalSequence analysisAdd
BLAST
Topological domaini225 – 27147CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: UniProtKB
  • basolateral plasma membrane Source: RGD
  • clathrin-coated vesicle Source: RGD
  • cytoplasm Source: RGD
  • cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  • early endosome Source: RGD
  • exocytic vesicle Source: RGD
  • integral component of plasma membrane Source: GO_Central
  • lysosome Source: RGD
  • plasma membrane Source: UniProtKB
  • protein complex Source: RGD
  • rough endoplasmic reticulum Source: RGD
  • trans-Golgi network Source: RGD
  • transport vesicle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 271271Aquaporin-2PRO_0000063940Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi124 – 1241N-linked (GlcNAc...)Sequence analysis
Modified residuei256 – 2561PhosphoserineCombined sources
Modified residuei261 – 2611PhosphoserineCombined sources
Modified residuei264 – 2641PhosphoserineCombined sources
Modified residuei269 – 2691PhosphoserineCombined sources

Post-translational modificationi

Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP34080.

PTM databases

iPTMnetiP34080.
PhosphoSiteiP34080.

Expressioni

Tissue specificityi

Expressed in renal collecting tubules.

Gene expression databases

BgeeiENSRNOG00000000297.

Interactioni

GO - Molecular functioni

  • actin binding Source: RGD
  • PDZ domain binding Source: RGD

Protein-protein interaction databases

DIPiDIP-46223N.
STRINGi10116.ENSRNOP00000000324.

Structurei

3D structure databases

ProteinModelPortaliP34080.
SMRiP34080. Positions 2-251.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi68 – 703NPA 1
Motifi184 – 1863NPA 2

Domaini

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0223. Eukaryota.
COG0580. LUCA.
HOGENOMiHOG000288286.
HOVERGENiHBG000312.
InParanoidiP34080.
KOiK09865.
PhylomeDBiP34080.
TreeFamiTF312940.

Family and domain databases

CDDicd00333. MIP. 1 hit.
Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34080-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWELRSIAFS RAVLAEFLAT LLFVFFGLGS ALQWASSPPS VLQIAVAFGL
60 70 80 90 100
GIGILVQALG HVSGAHINPA VTVACLVGCH VSFLRAAFYV AAQLLGAVAG
110 120 130 140 150
AAILHEITPV EIRGDLAVNA LHNNATAGQA VTVELFLTMQ LVLCIFASTD
160 170 180 190 200
ERRGDNLGSP ALSIGFSVTL GHLLGIYFTG CSMNPARSLA PAVVTGKFDD
210 220 230 240 250
HWVFWIGPLV GAIIGSLLYN YLLFPSAKSL QERLAVLKGL EPDTDWEERE
260 270
VRRRQSVELH SPQSLPRGSK A
Length:271
Mass (Da):28,931
Last modified:February 1, 1994 - v1
Checksum:i86722ED2BCE0B3E4
GO

Sequence cautioni

The sequence AAA41478 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti54 – 541I → T in AAI28706 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13906 mRNA. Translation: BAA03006.1.
L28112 mRNA. Translation: AAA41478.1. Different initiation.
BC128705 mRNA. Translation: AAI28706.1.
PIRiJT0750.
RefSeqiNP_037041.2. NM_012909.2.
UniGeneiRn.90076.

Genome annotation databases

GeneIDi25386.
KEGGirno:25386.
UCSCiRGD:2142. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13906 mRNA. Translation: BAA03006.1.
L28112 mRNA. Translation: AAA41478.1. Different initiation.
BC128705 mRNA. Translation: AAI28706.1.
PIRiJT0750.
RefSeqiNP_037041.2. NM_012909.2.
UniGeneiRn.90076.

3D structure databases

ProteinModelPortaliP34080.
SMRiP34080. Positions 2-251.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46223N.
STRINGi10116.ENSRNOP00000000324.

PTM databases

iPTMnetiP34080.
PhosphoSiteiP34080.

Proteomic databases

PaxDbiP34080.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25386.
KEGGirno:25386.
UCSCiRGD:2142. rat.

Organism-specific databases

CTDi359.
RGDi2142. Aqp2.

Phylogenomic databases

eggNOGiKOG0223. Eukaryota.
COG0580. LUCA.
HOGENOMiHOG000288286.
HOVERGENiHBG000312.
InParanoidiP34080.
KOiK09865.
PhylomeDBiP34080.
TreeFamiTF312940.

Miscellaneous databases

PROiP34080.

Gene expression databases

BgeeiENSRNOG00000000297.

Family and domain databases

CDDicd00333. MIP. 1 hit.
Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAQP2_RAT
AccessioniPrimary (citable) accession number: P34080
Secondary accession number(s): A1A5L4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: September 7, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.