P34078 (LTV1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein LTV1

Alternative name(s):
Low-temperature viability protein 1

Gene names

Name:	LTV1
Ordered Locus Names:	YKL143W
ORF Names:	YKL2

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in protein transport. Non-ribosomal factor required for efficient nuclear export of the ribosomal 40S subunit. Component of the GSE complex, a GTPase complex required for intracellular sorting of GAP1 out of the endosome. Ref.6 Ref.9
Subunit structure	Associates with the pre-40S ribosomal subunit. Component of the GSE complex composed of GTR1, GTR2, SLM4, MEH1 and LTV1. Interacts directly with GTR1. Interacts with CRM1. Ref.7 Ref.8 Ref.9
Subcellular location	Nucleus. Cytoplasm. Note: Shuttles between the nucleus and the cytoplasm. Ref.4 Ref.9
Post-translational modification	Phosphorylated, leading to its dissociation from the 40S pre-40S ribosome. Ref.7
Miscellaneous	Present with 589 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the LTV1 family.
Sequence caution	The sequence CAA80955.1 differs from that shown. Reason: Frameshift at positions 134 and 145.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Nucleus
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to oxidative stress Inferred from mutant phenotype Ref.6. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW response to osmotic stress Inferred from mutant phenotype Ref.6. Source: SGD ribosomal small subunit biogenesis Inferred from mutant phenotype Ref.6. Source: SGD ribosomal small subunit export from nucleus Inferred from mutant phenotype Ref.9. Source: SGD
Cellular_component	late endosome membrane Inferred from direct assay Ref.8. Source: SGD nucleus Inferred from direct assay Ref.9. Source: SGD preribosome, small subunit precursor Inferred from direct assay Ref.7. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CRM1	P30822	1	EBI-10248,EBI-20589
NUP116	Q02630	1	EBI-10248,EBI-11703
RPS3	P05750	1	EBI-10248,EBI-16140

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	Protein LTV1		PRO_0000084522
Regions
	Coiled coil	355 – 462	108	Potential
	Motif	168 – 179	12	Nuclear export signal
Amino acid modifications
	Modified residue	45	1	Phosphoserine Ref.10
	Modified residue	303	1	Phosphoserine Ref.10

Sequences

	Sequence		Length	Mass (Da)
	P34078 [UniParc]. Last modified June 1, 1994. Version 2. Checksum: F432C239B3B0A68A	FASTA	463	53,390
	10 20 30 40 50 60 MSKKFSSKNS QRYVVVHRPH DDPSFYDTDA SAHVLVPVSN PNKTSPEADL RKKDVSSTKP 70 80 90 100 110 120 KGRRAHVGEA ALYGINFDDS EYDYTQHLKP IGLDPENSIF IASKGNEQKV EKKNIEDLFI 130 140 150 160 170 180 EPKYRRDEIE KDDALPVFQR GMAKPEYLLH QQDTTDEIRG FKPDMNPALR EVLEALEDEA 190 200 210 220 230 240 YVVNDDVVVE DISKKTQLQG DNYGEEEKED DIFAQLLGSG EAKDEDEFED EFDEWDIDNV 250 260 270 280 290 300 ENFEDENYVK EMAQFDNIEN LEDLENIDYQ ADVRRFQKDN SILEKHNSDD EFSNAGLDSV 310 320 330 340 350 360 NPSEEEDVLG ELPSIQDKSK TGKKKRKSRQ KKGAMSDVSG FSMSSSAIAR TETMTVLDDQ 370 380 390 400 410 420 YDQIINGYEN YEEELEEDEE QNYQPFDMSA ERSDFESMLD DFLDNYELES GGRKLAKKDK 430 440 450 460 EIERLKEAAD EVSKGKLSQR RNRERQEKKK LEKVTNTLSS LKF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and physical analysis of an 8.2 kb segment of chromosome XI of Saccharomyces cerevisiae reveals five tightly linked genes." Abraham P.R., Mulder A., Van'T Riet J., Planta R.J., Raue H.A. Yeast 8:227-238(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Genetic and biochemical interactions among Yar1, Ltv1 and Rps3 define novel links between environmental stress and ribosome biogenesis in Saccharomyces cerevisiae." Loar J.W., Seiser R.M., Sundberg A.E., Sagerson H.J., Ilias N., Zobel-Thropp P., Craig E.A., Lycan D.E. Genetics 168:1877-1889(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Hrr25-dependent phosphorylation state regulates organization of the pre-40S subunit." Schaefer T., Maco B., Petfalski E., Tollervey D., Boettcher B., Aebi U., Hurt E. Nature 441:651-655(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH THE PRE-40S RIBOSOME, PHOSPHORYLATION.
[8]	"A conserved GTPase-containing complex is required for intracellular sorting of the general amino-acid permease in yeast." Gao M., Kaiser C.A. Nat. Cell Biol. 8:657-667(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE GSE COMPLEX, INTERACTION WITH GTR1.
[9]	"Ltv1 is required for efficient nuclear export of the ribosomal small subunit in Saccharomyces cerevisiae." Seiser R.M., Sundberg A.E., Wollam B.J., Zobel-Thropp P., Baldwin K., Spector M.D., Lycan D.E. Genetics 174:679-691(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, NUCLEAR EXPORT SIGNAL, INTERACTION WITH CRM1.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45 AND SER-303, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z25464 Genomic DNA. Translation: CAA80955.1. Frameshift. Z28143 Genomic DNA. Translation: CAA81984.1. BK006944 Genomic DNA. Translation: DAA09019.1.
PIR	S37972.
RefSeq	NP_012779.1. NM_001179709.1.
3D structure databases
ProteinModelPortal	P34078.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2801N.
IntAct	P34078. 9 interactions.
MINT	MINT-512519.
STRING	4932.YKL143W.
Proteomic databases
PaxDb	P34078.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL143W; YKL143W; YKL143W.
GeneID	853714.
KEGG	sce:YKL143W.
Organism-specific databases
CYGD	YKL143w.
SGD	S000001626. LTV1.
Phylogenomic databases
eggNOG	NOG263248.
HOGENOM	HOG000172553.
KO	K14798.
OMA	ALEDEAY.
OrthoDB	EOG412QDZ.
Enzyme and pathway databases
BioCyc	YEAST:G3O-31918-MONOMER.
Gene expression databases
Genevestigator	P34078.
GermOnline	YKL143W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007307. LTV. [Graphical view]
Pfam	PF04180. LTV. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974725.

Entry information

Entry name

LTV1_YEAST

Accession

Primary (citable) accession number: P34078
Secondary accession number(s): D6VX53

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 29, 2013
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program