Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nucleoporin NIC96

Gene

NIC96

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. NIC96, which is localized to the core of the NPC and the distal ring of the nuclear basket, is required for de novo assembly of NPCs. It is involved in nuclear GSP1 import.11 Publications

GO - Molecular functioni

  • structural constituent of nuclear pore Source: SGD

GO - Biological processi

  • nuclear pore complex assembly Source: GO_Central
  • nuclear pore organization Source: SGD
  • poly(A)+ mRNA export from nucleus Source: GO_Central
  • protein import into nucleus Source: SGD
  • ribosomal large subunit export from nucleus Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30455-MONOMER.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin NIC96
Alternative name(s):
96 kDa nucleoporin-interacting component
Nuclear pore protein NIC96
Gene namesi
Name:NIC96
Ordered Locus Names:YFR002W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFR002W.
SGDiS000001898. NIC96.

Subcellular locationi

GO - Cellular componenti

  • nuclear membrane Source: UniProtKB-SubCell
  • nuclear pore Source: SGD
  • nuclear pore linkers Source: SGD
  • nuclear pore nuclear basket Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001247871 – 839Nucleoporin NIC96Add BLAST839

Proteomic databases

MaxQBiP34077.
PRIDEiP34077.

PTM databases

iPTMnetiP34077.

Interactioni

Subunit structurei

The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEH1 and SEC1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. NIC96 is part of three NPC subcomplexes, interacting with NSP1 of the NUP57 subcomplex (NIC96, NSP1, NUP49, NUP57), with NUP120 of the NUP84 subcomplex (SEH1,NUP85, NUP120, NUP145C, SEC13, NUP84, NUP133), and with NUP53 of the NUP53-NUP59-NUP170 subcomplex. The interaction with NUP53 is cell cycle dependent. NIC96 is also associated with the distal ring of the nuclear basket and interacts here with MLP2, which forms together with MLP1 nuclear pore-attached intranuclear filaments.8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NUP192P470543EBI-12056,EBI-25846
NUP49Q021993EBI-12056,EBI-12315
NUP53Q037903EBI-12056,EBI-27321
NUP57P488375EBI-12056,EBI-12324

Protein-protein interaction databases

BioGridi31150. 81 interactors.
DIPiDIP-745N.
IntActiP34077. 31 interactors.
MINTiMINT-398487.

Structurei

Secondary structure

1839
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi206 – 227Combined sources22
Helixi234 – 243Combined sources10
Helixi248 – 261Combined sources14
Turni262 – 265Combined sources4
Helixi270 – 293Combined sources24
Helixi301 – 311Combined sources11
Beta strandi312 – 314Combined sources3
Beta strandi316 – 318Combined sources3
Beta strandi320 – 322Combined sources3
Helixi333 – 341Combined sources9
Turni342 – 344Combined sources3
Helixi346 – 355Combined sources10
Helixi357 – 359Combined sources3
Helixi367 – 373Combined sources7
Turni375 – 378Combined sources4
Beta strandi384 – 387Combined sources4
Helixi388 – 397Combined sources10
Turni398 – 400Combined sources3
Beta strandi402 – 404Combined sources3
Helixi406 – 416Combined sources11
Helixi420 – 422Combined sources3
Helixi426 – 428Combined sources3
Helixi432 – 441Combined sources10
Beta strandi449 – 452Combined sources4
Helixi459 – 469Combined sources11
Helixi471 – 474Combined sources4
Helixi478 – 484Combined sources7
Helixi488 – 496Combined sources9
Helixi500 – 512Combined sources13
Helixi534 – 542Combined sources9
Turni543 – 548Combined sources6
Helixi550 – 558Combined sources9
Helixi559 – 562Combined sources4
Helixi566 – 583Combined sources18
Helixi586 – 590Combined sources5
Beta strandi595 – 597Combined sources3
Helixi603 – 606Combined sources4
Helixi609 – 611Combined sources3
Helixi616 – 633Combined sources18
Helixi637 – 646Combined sources10
Helixi650 – 667Combined sources18
Beta strandi675 – 679Combined sources5
Turni681 – 683Combined sources3
Helixi685 – 696Combined sources12
Helixi700 – 703Combined sources4
Helixi708 – 728Combined sources21
Helixi732 – 741Combined sources10
Helixi750 – 753Combined sources4
Helixi754 – 759Combined sources6
Helixi760 – 762Combined sources3
Helixi765 – 768Combined sources4
Helixi771 – 790Combined sources20
Helixi799 – 819Combined sources21
Helixi820 – 823Combined sources4
Helixi826 – 833Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QX5X-ray2.50A/B186-839[»]
2RFOX-ray2.60A/B189-839[»]
ProteinModelPortaliP34077.
SMRiP34077.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP34077.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni25 – 60Leucine zipper-like heptad repeat, required for interaction with NSP1Add BLAST36

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi187 – 236Asn-richAdd BLAST50

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000016353.
HOGENOMiHOG000093574.
InParanoidiP34077.
KOiK14309.
OMAiHTEYNQH.
OrthoDBiEOG092C0JRR.

Family and domain databases

InterProiIPR007231. Nucleoporin_int_Nup93/Nic96.
[Graphical view]
PANTHERiPTHR11225. PTHR11225. 1 hit.
PfamiPF04097. Nic96. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34077-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLETLRGNKL HSGTSKGANK KLNELLESSD NLPSASSELG SIQVSINELR
60 70 80 90 100
RRVFQLRSKN KASKDYTKAH YLLANSGLSF EDVDAFIKDL QTNQFLEPNP
110 120 130 140 150
PKIIESEELE FYIRTKKEEN ILMSIEQLLN GATKDFDNFI NHNLNLDWAQ
160 170 180 190 200
HKNEVMKNFG ILIQDKKTVD HKKSISSLDP KLPSWGNKGN NILNSNESRL
210 220 230 240 250
NVNENNILRE KFENYARIVF QFNNSRQANG NFDIANEFIS ILSSANGTRN
260 270 280 290 300
AQLLESWKIL ESMKSKDINI VEVGKQYLEQ QFLQYTDNLY KKNMNEGLAT
310 320 330 340 350
NVNKIKSFID TKLKKADKSW KISNLTVING VPIWALIFYL LRAGLIKEAL
360 370 380 390 400
QVLVENKANI KKVEQSFLTY FKAYASSKDH GLPVEYSTKL HTEYNQHIKS
410 420 430 440 450
SLDGDPYRLA VYKLIGRCDL SRKNIPAVTL SIEDWLWMHL MLIKEKDAEN
460 470 480 490 500
DPVYERYSLE DFQNIIISYG PSRFSNYYLQ TLLLSGLYGL AIDYTYTFSE
510 520 530 540 550
MDAVHLAIGL ASLKLFKIDS STRLTKKPKR DIRFANILAN YTKSFRYSDP
560 570 580 590 600
RVAVEYLVLI TLNEGPTDVE LCHEALRELV LETKEFTVLL GKIGRDGARI
610 620 630 640 650
PGVIEERQPL LHVRDEKEFL HTITEQAARR ADEDGRIYDS ILLYQLAEEY
660 670 680 690 700
DIVITLVNSL LSDTLSASDL DQPLVGPDDN SETNPVLLAR RMASIYFDNA
710 720 730 740 750
GISRQIHVKN KEICMLLLNI SSIRELYFNK QWQETLSQME LLDLLPFSDE
760 770 780 790 800
LSARKKAQDF SNLDDNIVKN IPNLLIITLS CISNMIHILN ESKYQSSTKG
810 820 830
QQIDSLKNVA RQCMIYAGMI QYRMPRETYS TLINIDVSL
Length:839
Mass (Da):96,174
Last modified:November 1, 1995 - v2
Checksum:i48EC3BC71281CB44
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti59Missing in CAA51427 (PubMed:7688296).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72923 Genomic DNA. Translation: CAA51427.1.
D50617 Genomic DNA. Translation: BAA09241.1.
BK006940 Genomic DNA. Translation: DAA12442.1.
PIRiS35319.
RefSeqiNP_116657.1. NM_001179967.1.

Genome annotation databases

EnsemblFungiiBAA09241; BAA09241; BAA09241.
YFR002W; YFR002W; YFR002W.
GeneIDi850552.
KEGGisce:YFR002W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72923 Genomic DNA. Translation: CAA51427.1.
D50617 Genomic DNA. Translation: BAA09241.1.
BK006940 Genomic DNA. Translation: DAA12442.1.
PIRiS35319.
RefSeqiNP_116657.1. NM_001179967.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QX5X-ray2.50A/B186-839[»]
2RFOX-ray2.60A/B189-839[»]
ProteinModelPortaliP34077.
SMRiP34077.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31150. 81 interactors.
DIPiDIP-745N.
IntActiP34077. 31 interactors.
MINTiMINT-398487.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

PTM databases

iPTMnetiP34077.

Proteomic databases

MaxQBiP34077.
PRIDEiP34077.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA09241; BAA09241; BAA09241.
YFR002W; YFR002W; YFR002W.
GeneIDi850552.
KEGGisce:YFR002W.

Organism-specific databases

EuPathDBiFungiDB:YFR002W.
SGDiS000001898. NIC96.

Phylogenomic databases

GeneTreeiENSGT00390000016353.
HOGENOMiHOG000093574.
InParanoidiP34077.
KOiK14309.
OMAiHTEYNQH.
OrthoDBiEOG092C0JRR.

Enzyme and pathway databases

BioCyciYEAST:G3O-30455-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP34077.
PROiP34077.

Family and domain databases

InterProiIPR007231. Nucleoporin_int_Nup93/Nic96.
[Graphical view]
PANTHERiPTHR11225. PTHR11225. 1 hit.
PfamiPF04097. Nic96. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNIC96_YEAST
AccessioniPrimary (citable) accession number: P34077
Secondary accession number(s): D6VTN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 157 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 15500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.