ID   MKS1_YEAST              Reviewed;         584 AA.
AC   P34072; D6W1A3; Q02533;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 2.
DT   27-MAR-2024, entry version 176.
DE   RecName: Full=Negative regulator of RAS-cAMP pathway;
GN   Name=MKS1; Synonyms=LYS80; OrderedLocusNames=YNL076W;
GN   ORFNames=N2344, N2347;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 96604 / S288c / FY1679;
RX   PubMed=8701611;
RX   DOI=10.1002/(sici)1097-0061(19960330)12:4<391::aid-yea921>3.0.co;2-n;
RA   Poehlmann R., Philippsen P.;
RT   "Sequencing a cosmid clone of Saccharomyces cerevisiae chromosome XIV
RT   reveals 12 new open reading frames (ORFs) and an ancient duplication of six
RT   ORFs.";
RL   Yeast 12:391-402(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169873;
RA   Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA   Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA   Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA   Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA   Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA   Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA   Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA   Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA   Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA   Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA   Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA   Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA   Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA   Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA   Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA   Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA   Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT   evolutionary implications.";
RL   Nature 387:93-98(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 127-584.
RX   PubMed=8386801; DOI=10.1007/bf00279524;
RA   Matsuura A., Anraku Y.;
RT   "Characterization of the MKS1 gene, a new negative regulator of the Ras-
RT   cyclic AMP pathway in Saccharomyces cerevisiae.";
RL   Mol. Gen. Genet. 238:6-16(1993).
RN   [5]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-442, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=YAL6B;
RX   PubMed=15665377; DOI=10.1074/mcp.m400219-mcp200;
RA   Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M.,
RA   Jensen O.N.;
RT   "Quantitative phosphoproteomics applied to the yeast pheromone signaling
RT   pathway.";
RL   Mol. Cell. Proteomics 4:310-327(2005).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-247, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ADR376;
RX   PubMed=17330950; DOI=10.1021/pr060559j;
RA   Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA   Elias J.E., Gygi S.P.;
RT   "Large-scale phosphorylation analysis of alpha-factor-arrested
RT   Saccharomyces cerevisiae.";
RL   J. Proteome Res. 6:1190-1197(2007).
RN   [7]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=17287358; DOI=10.1073/pnas.0607084104;
RA   Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,
RA   Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.;
RT   "Analysis of phosphorylation sites on proteins from Saccharomyces
RT   cerevisiae by electron transfer dissociation (ETD) mass spectrometry.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007).
RN   [8]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-25; SER-247 AND SER-276, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth phosphoproteome
RT   analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
RN   [9]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-442, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19779198; DOI=10.1126/science.1172867;
RA   Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT   "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT   into evolution.";
RL   Science 325:1682-1686(2009).
CC   -!- FUNCTION: Negative regulator of Ras-cAMP pathway. Involved in
CC       transcriptional regulation of galactose-inducible genes.
CC   -!- INTERACTION:
CC       P34072; P35169: TOR1; NbExp=3; IntAct=EBI-10978, EBI-19374;
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA60181.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; X86470; CAA60181.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; X86470; CAA60182.1; -; Genomic_DNA.
DR   EMBL; Z71352; CAA95950.1; -; Genomic_DNA.
DR   EMBL; D13715; BAA02868.1; -; Genomic_DNA.
DR   EMBL; BK006947; DAA10469.1; -; Genomic_DNA.
DR   PIR; S53902; S53902.
DR   RefSeq; NP_014323.1; NM_001182914.1.
DR   AlphaFoldDB; P34072; -.
DR   SMR; P34072; -.
DR   BioGRID; 35747; 386.
DR   DIP; DIP-6677N; -.
DR   IntAct; P34072; 19.
DR   MINT; P34072; -.
DR   STRING; 4932.YNL076W; -.
DR   GlyGen; P34072; 8 sites, 1 O-linked glycan (8 sites).
DR   iPTMnet; P34072; -.
DR   MaxQB; P34072; -.
DR   PaxDb; 4932-YNL076W; -.
DR   PeptideAtlas; P34072; -.
DR   EnsemblFungi; YNL076W_mRNA; YNL076W; YNL076W.
DR   GeneID; 855648; -.
DR   KEGG; sce:YNL076W; -.
DR   AGR; SGD:S000005020; -.
DR   SGD; S000005020; MKS1.
DR   VEuPathDB; FungiDB:YNL076W; -.
DR   eggNOG; ENOG502RFNU; Eukaryota.
DR   HOGENOM; CLU_023053_0_0_1; -.
DR   InParanoid; P34072; -.
DR   OMA; MDIPSKN; -.
DR   OrthoDB; 1334409at2759; -.
DR   BioCyc; YEAST:G3O-33105-MONOMER; -.
DR   BioGRID-ORCS; 855648; 0 hits in 10 CRISPR screens.
DR   PRO; PR:P34072; -.
DR   Proteomes; UP000002311; Chromosome XIV.
DR   RNAct; P34072; Protein.
DR   GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0031930; P:mitochondria-nucleus signaling pathway; IPI:SGD.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:SGD.
DR   GO; GO:0006808; P:regulation of nitrogen utilization; IMP:SGD.
DR   GO; GO:2000220; P:regulation of pseudohyphal growth; IMP:SGD.
DR   InterPro; IPR013860; AreA_GATA.
DR   PANTHER; PTHR28014; NEGATIVE REGULATOR OF RAS-CAMP PATHWAY; 1.
DR   PANTHER; PTHR28014:SF1; NEGATIVE REGULATOR OF RAS-CAMP PATHWAY; 1.
DR   Pfam; PF08550; DUF1752; 1.
PE   1: Evidence at protein level;
KW   Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..584
FT                   /note="Negative regulator of RAS-cAMP pathway"
FT                   /id="PRO_0000096494"
FT   REGION          95..167
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          209..279
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          291..320
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          343..366
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          381..432
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          551..584
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        110..128
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        139..167
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        209..254
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        263..279
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        294..320
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         25
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0007744|PubMed:18407956"
FT   MOD_RES         247
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:17330950,
FT                   ECO:0007744|PubMed:18407956"
FT   MOD_RES         276
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:18407956"
FT   MOD_RES         442
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:15665377,
FT                   ECO:0007744|PubMed:19779198"
FT   MOD_RES         518
FT                   /note="Phosphoserine; by PKA"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   584 AA;  65643 MW;  79B0EE5F8DDE0145 CRC64;
     MSREAFDVPN IGTNKFLKVT PNLFTPERLN LFDDVELYLT LIKASKCVEQ GERLHNISWR
     ILNKAVLKEH NINRSKKRDG VKNIYYVLNP NNKQPIKPKQ AAVKQPPLQK ANLPPTTAKQ
     NVLTRPMTSP AIAQGAHDRS LDNPNSTNND VKNDVAPNRQ FSKSTTSGLF SNFADKYQKM
     KNVNHVANKE EPQTIITGFD TSTVITKKPL QSRRSRSPFQ HIGDMNMNCI DNETSKSTSP
     TLENMGSRKS SFPQKESLFG RPRSYKNDQN GQLSLSKTSS RKGKNKIFFS SEDEDSDWDS
     VSNDSEFYAD EDDEEYDDYN EEEADQYYRR QWDKLLFAKN QQNLDSTKSS VSSANTINSN
     TSHDPVRKSL LSGLFLSEAN SNSNNHNTAH SEYASKHVSP TPQSSHSNIG PQPQQNPPSA
     NGIKQQKPSL KTSNVTALAS LSPPQPSNNE RLSMDIQKDF KTDNESNHLY ESNAPLTAQT
     ILPTALSTHM FLPNNIHQQR MAIATGSNTR HRFSRRQSMD IPSKNRNTGF LKTRMEISEE
     EKMVRTISRL DNTSIANSNG NGNDDTSNQR TEALGRKTSN GGRI
//