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P34072

- MKS1_YEAST

UniProt

P34072 - MKS1_YEAST

Protein

Negative regulator of RAS-cAMP pathway

Gene

MKS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Negative regulator of Ras-cAMP pathway. Involved in transcriptional regulation of galactose-inducible genes.

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. mitochondria-nucleus signaling pathway Source: SGD
    2. negative regulation of transcription from RNA polymerase II promoter Source: SGD
    3. regulation of nitrogen utilization Source: SGD
    4. regulation of pseudohyphal growth Source: SGD
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33105-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Negative regulator of RAS-cAMP pathway
    Gene namesi
    Name:MKS1
    Synonyms:LYS80
    Ordered Locus Names:YNL076W
    ORF Names:N2344, N2347
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL076w.
    SGDiS000005020. MKS1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 584584Negative regulator of RAS-cAMP pathwayPRO_0000096494Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei25 – 251Phosphothreonine1 Publication
    Modified residuei247 – 2471Phosphoserine2 Publications
    Modified residuei276 – 2761Phosphoserine1 Publication
    Modified residuei442 – 4421Phosphoserine2 Publications
    Modified residuei518 – 5181Phosphoserine; by PKASequence Analysis

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP34072.
    PaxDbiP34072.

    Expressioni

    Gene expression databases

    GenevestigatoriP34072.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TOR1P351693EBI-10978,EBI-19374

    Protein-protein interaction databases

    BioGridi35747. 144 interactions.
    DIPiDIP-6677N.
    IntActiP34072. 18 interactions.
    MINTiMINT-650804.
    STRINGi4932.YNL076W.

    Structurei

    3D structure databases

    ProteinModelPortaliP34072.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG71686.
    HOGENOMiHOG000113596.
    OMAiSTHMFLP.
    OrthoDBiEOG7F252T.

    Family and domain databases

    InterProiIPR013860. DUF1752_fun.
    [Graphical view]
    PfamiPF08550. DUF1752. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P34072-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSREAFDVPN IGTNKFLKVT PNLFTPERLN LFDDVELYLT LIKASKCVEQ    50
    GERLHNISWR ILNKAVLKEH NINRSKKRDG VKNIYYVLNP NNKQPIKPKQ 100
    AAVKQPPLQK ANLPPTTAKQ NVLTRPMTSP AIAQGAHDRS LDNPNSTNND 150
    VKNDVAPNRQ FSKSTTSGLF SNFADKYQKM KNVNHVANKE EPQTIITGFD 200
    TSTVITKKPL QSRRSRSPFQ HIGDMNMNCI DNETSKSTSP TLENMGSRKS 250
    SFPQKESLFG RPRSYKNDQN GQLSLSKTSS RKGKNKIFFS SEDEDSDWDS 300
    VSNDSEFYAD EDDEEYDDYN EEEADQYYRR QWDKLLFAKN QQNLDSTKSS 350
    VSSANTINSN TSHDPVRKSL LSGLFLSEAN SNSNNHNTAH SEYASKHVSP 400
    TPQSSHSNIG PQPQQNPPSA NGIKQQKPSL KTSNVTALAS LSPPQPSNNE 450
    RLSMDIQKDF KTDNESNHLY ESNAPLTAQT ILPTALSTHM FLPNNIHQQR 500
    MAIATGSNTR HRFSRRQSMD IPSKNRNTGF LKTRMEISEE EKMVRTISRL 550
    DNTSIANSNG NGNDDTSNQR TEALGRKTSN GGRI 584
    Length:584
    Mass (Da):65,643
    Last modified:February 1, 1996 - v2
    Checksum:i79B0EE5F8DDE0145
    GO

    Sequence cautioni

    The sequence CAA60181.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X86470 Genomic DNA. Translation: CAA60181.1. Sequence problems.
    X86470 Genomic DNA. Translation: CAA60182.1.
    Z71352 Genomic DNA. Translation: CAA95950.1.
    D13715 Genomic DNA. Translation: BAA02868.1.
    BK006947 Genomic DNA. Translation: DAA10469.1.
    PIRiS53902.
    RefSeqiNP_014323.1. NM_001182914.1.

    Genome annotation databases

    EnsemblFungiiYNL076W; YNL076W; YNL076W.
    GeneIDi855648.
    KEGGisce:YNL076W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X86470 Genomic DNA. Translation: CAA60181.1 . Sequence problems.
    X86470 Genomic DNA. Translation: CAA60182.1 .
    Z71352 Genomic DNA. Translation: CAA95950.1 .
    D13715 Genomic DNA. Translation: BAA02868.1 .
    BK006947 Genomic DNA. Translation: DAA10469.1 .
    PIRi S53902.
    RefSeqi NP_014323.1. NM_001182914.1.

    3D structure databases

    ProteinModelPortali P34072.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35747. 144 interactions.
    DIPi DIP-6677N.
    IntActi P34072. 18 interactions.
    MINTi MINT-650804.
    STRINGi 4932.YNL076W.

    Proteomic databases

    MaxQBi P34072.
    PaxDbi P34072.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL076W ; YNL076W ; YNL076W .
    GeneIDi 855648.
    KEGGi sce:YNL076W.

    Organism-specific databases

    CYGDi YNL076w.
    SGDi S000005020. MKS1.

    Phylogenomic databases

    eggNOGi NOG71686.
    HOGENOMi HOG000113596.
    OMAi STHMFLP.
    OrthoDBi EOG7F252T.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33105-MONOMER.

    Miscellaneous databases

    NextBioi 979890.

    Gene expression databases

    Genevestigatori P34072.

    Family and domain databases

    InterProi IPR013860. DUF1752_fun.
    [Graphical view ]
    Pfami PF08550. DUF1752. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing a cosmid clone of Saccharomyces cerevisiae chromosome XIV reveals 12 new open reading frames (ORFs) and an ancient duplication of six ORFs."
      Poehlmann R., Philippsen P.
      Yeast 12:391-402(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Characterization of the MKS1 gene, a new negative regulator of the Ras-cyclic AMP pathway in Saccharomyces cerevisiae."
      Matsuura A., Anraku Y.
      Mol. Gen. Genet. 238:6-16(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 127-584.
    5. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-442, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-247, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-25; SER-247 AND SER-276, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-442, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMKS1_YEAST
    AccessioniPrimary (citable) accession number: P34072
    Secondary accession number(s): D6W1A3, Q02533
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3