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P34072 (MKS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Negative regulator of RAS-cAMP pathway
Gene names
Name:MKS1
Synonyms:LYS80
Ordered Locus Names:YNL076W
ORF Names:N2344, N2347
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length584 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Negative regulator of Ras-cAMP pathway. Involved in transcriptional regulation of galactose-inducible genes.

Subcellular location

Nucleus.

Sequence caution

The sequence CAA60181.1 differs from that shown. Reason: Erroneous gene model prediction.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

TOR1P351693EBI-10978,EBI-19374

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 584584Negative regulator of RAS-cAMP pathway
PRO_0000096494

Amino acid modifications

Modified residue251Phosphothreonine Ref.8
Modified residue2471Phosphoserine Ref.6 Ref.8
Modified residue2761Phosphoserine Ref.8
Modified residue4421Phosphoserine Ref.5 Ref.9
Modified residue5181Phosphoserine; by PKA Potential

Sequences

Sequence LengthMass (Da)Tools
P34072 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 79B0EE5F8DDE0145

FASTA58465,643
        10         20         30         40         50         60 
MSREAFDVPN IGTNKFLKVT PNLFTPERLN LFDDVELYLT LIKASKCVEQ GERLHNISWR 

        70         80         90        100        110        120 
ILNKAVLKEH NINRSKKRDG VKNIYYVLNP NNKQPIKPKQ AAVKQPPLQK ANLPPTTAKQ 

       130        140        150        160        170        180 
NVLTRPMTSP AIAQGAHDRS LDNPNSTNND VKNDVAPNRQ FSKSTTSGLF SNFADKYQKM 

       190        200        210        220        230        240 
KNVNHVANKE EPQTIITGFD TSTVITKKPL QSRRSRSPFQ HIGDMNMNCI DNETSKSTSP 

       250        260        270        280        290        300 
TLENMGSRKS SFPQKESLFG RPRSYKNDQN GQLSLSKTSS RKGKNKIFFS SEDEDSDWDS 

       310        320        330        340        350        360 
VSNDSEFYAD EDDEEYDDYN EEEADQYYRR QWDKLLFAKN QQNLDSTKSS VSSANTINSN 

       370        380        390        400        410        420 
TSHDPVRKSL LSGLFLSEAN SNSNNHNTAH SEYASKHVSP TPQSSHSNIG PQPQQNPPSA 

       430        440        450        460        470        480 
NGIKQQKPSL KTSNVTALAS LSPPQPSNNE RLSMDIQKDF KTDNESNHLY ESNAPLTAQT 

       490        500        510        520        530        540 
ILPTALSTHM FLPNNIHQQR MAIATGSNTR HRFSRRQSMD IPSKNRNTGF LKTRMEISEE 

       550        560        570        580 
EKMVRTISRL DNTSIANSNG NGNDDTSNQR TEALGRKTSN GGRI 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing a cosmid clone of Saccharomyces cerevisiae chromosome XIV reveals 12 new open reading frames (ORFs) and an ancient duplication of six ORFs."
Poehlmann R., Philippsen P.
Yeast 12:391-402(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Characterization of the MKS1 gene, a new negative regulator of the Ras-cyclic AMP pathway in Saccharomyces cerevisiae."
Matsuura A., Anraku Y.
Mol. Gen. Genet. 238:6-16(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 127-584.
[5]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-442, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[6]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-247, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[7]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-25; SER-247 AND SER-276, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-442, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X86470 Genomic DNA. Translation: CAA60181.1. Sequence problems.
X86470 Genomic DNA. Translation: CAA60182.1.
Z71352 Genomic DNA. Translation: CAA95950.1.
D13715 Genomic DNA. Translation: BAA02868.1.
BK006947 Genomic DNA. Translation: DAA10469.1.
PIRS53902.
RefSeqNP_014323.1. NM_001182914.1.

3D structure databases

ProteinModelPortalP34072.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35747. 143 interactions.
DIPDIP-6677N.
IntActP34072. 18 interactions.
MINTMINT-650804.
STRING4932.YNL076W.

Proteomic databases

PaxDbP34072.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL076W; YNL076W; YNL076W.
GeneID855648.
KEGGsce:YNL076W.

Organism-specific databases

CYGDYNL076w.
SGDS000005020. MKS1.

Phylogenomic databases

eggNOGNOG71686.
HOGENOMHOG000113596.
OMATALSTHM.
OrthoDBEOG7F252T.

Enzyme and pathway databases

BioCycYEAST:G3O-33105-MONOMER.

Gene expression databases

GenevestigatorP34072.

Family and domain databases

InterProIPR013860. DUF1752_fun.
[Graphical view]
PfamPF08550. DUF1752. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979890.

Entry information

Entry nameMKS1_YEAST
AccessionPrimary (citable) accession number: P34072
Secondary accession number(s): D6W1A3, Q02533
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1996
Last modified: March 19, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD