Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P34072

- MKS1_YEAST

UniProt

P34072 - MKS1_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Negative regulator of RAS-cAMP pathway

Gene

MKS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Negative regulator of Ras-cAMP pathway. Involved in transcriptional regulation of galactose-inducible genes.

GO - Biological processi

  1. mitochondria-nucleus signaling pathway Source: SGD
  2. negative regulation of transcription from RNA polymerase II promoter Source: SGD
  3. regulation of nitrogen utilization Source: SGD
  4. regulation of pseudohyphal growth Source: SGD
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-33105-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Negative regulator of RAS-cAMP pathway
Gene namesi
Name:MKS1
Synonyms:LYS80
Ordered Locus Names:YNL076W
ORF Names:N2344, N2347
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL076w.
SGDiS000005020. MKS1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 584584Negative regulator of RAS-cAMP pathwayPRO_0000096494Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei25 – 251Phosphothreonine1 Publication
Modified residuei247 – 2471Phosphoserine2 Publications
Modified residuei276 – 2761Phosphoserine1 Publication
Modified residuei442 – 4421Phosphoserine2 Publications
Modified residuei518 – 5181Phosphoserine; by PKASequence Analysis

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP34072.
PaxDbiP34072.

Expressioni

Gene expression databases

GenevestigatoriP34072.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
TOR1P351693EBI-10978,EBI-19374

Protein-protein interaction databases

BioGridi35747. 144 interactions.
DIPiDIP-6677N.
IntActiP34072. 18 interactions.
MINTiMINT-650804.
STRINGi4932.YNL076W.

Structurei

3D structure databases

ProteinModelPortaliP34072.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiNOG71686.
HOGENOMiHOG000113596.
InParanoidiP34072.
OMAiSTHMFLP.
OrthoDBiEOG7F252T.

Family and domain databases

InterProiIPR013860. DUF1752_fun.
[Graphical view]
PfamiPF08550. DUF1752. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34072-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSREAFDVPN IGTNKFLKVT PNLFTPERLN LFDDVELYLT LIKASKCVEQ
60 70 80 90 100
GERLHNISWR ILNKAVLKEH NINRSKKRDG VKNIYYVLNP NNKQPIKPKQ
110 120 130 140 150
AAVKQPPLQK ANLPPTTAKQ NVLTRPMTSP AIAQGAHDRS LDNPNSTNND
160 170 180 190 200
VKNDVAPNRQ FSKSTTSGLF SNFADKYQKM KNVNHVANKE EPQTIITGFD
210 220 230 240 250
TSTVITKKPL QSRRSRSPFQ HIGDMNMNCI DNETSKSTSP TLENMGSRKS
260 270 280 290 300
SFPQKESLFG RPRSYKNDQN GQLSLSKTSS RKGKNKIFFS SEDEDSDWDS
310 320 330 340 350
VSNDSEFYAD EDDEEYDDYN EEEADQYYRR QWDKLLFAKN QQNLDSTKSS
360 370 380 390 400
VSSANTINSN TSHDPVRKSL LSGLFLSEAN SNSNNHNTAH SEYASKHVSP
410 420 430 440 450
TPQSSHSNIG PQPQQNPPSA NGIKQQKPSL KTSNVTALAS LSPPQPSNNE
460 470 480 490 500
RLSMDIQKDF KTDNESNHLY ESNAPLTAQT ILPTALSTHM FLPNNIHQQR
510 520 530 540 550
MAIATGSNTR HRFSRRQSMD IPSKNRNTGF LKTRMEISEE EKMVRTISRL
560 570 580
DNTSIANSNG NGNDDTSNQR TEALGRKTSN GGRI
Length:584
Mass (Da):65,643
Last modified:February 1, 1996 - v2
Checksum:i79B0EE5F8DDE0145
GO

Sequence cautioni

The sequence CAA60181.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86470 Genomic DNA. Translation: CAA60181.1. Sequence problems.
X86470 Genomic DNA. Translation: CAA60182.1.
Z71352 Genomic DNA. Translation: CAA95950.1.
D13715 Genomic DNA. Translation: BAA02868.1.
BK006947 Genomic DNA. Translation: DAA10469.1.
PIRiS53902.
RefSeqiNP_014323.1. NM_001182914.1.

Genome annotation databases

EnsemblFungiiYNL076W; YNL076W; YNL076W.
GeneIDi855648.
KEGGisce:YNL076W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86470 Genomic DNA. Translation: CAA60181.1 . Sequence problems.
X86470 Genomic DNA. Translation: CAA60182.1 .
Z71352 Genomic DNA. Translation: CAA95950.1 .
D13715 Genomic DNA. Translation: BAA02868.1 .
BK006947 Genomic DNA. Translation: DAA10469.1 .
PIRi S53902.
RefSeqi NP_014323.1. NM_001182914.1.

3D structure databases

ProteinModelPortali P34072.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35747. 144 interactions.
DIPi DIP-6677N.
IntActi P34072. 18 interactions.
MINTi MINT-650804.
STRINGi 4932.YNL076W.

Proteomic databases

MaxQBi P34072.
PaxDbi P34072.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL076W ; YNL076W ; YNL076W .
GeneIDi 855648.
KEGGi sce:YNL076W.

Organism-specific databases

CYGDi YNL076w.
SGDi S000005020. MKS1.

Phylogenomic databases

eggNOGi NOG71686.
HOGENOMi HOG000113596.
InParanoidi P34072.
OMAi STHMFLP.
OrthoDBi EOG7F252T.

Enzyme and pathway databases

BioCyci YEAST:G3O-33105-MONOMER.

Miscellaneous databases

NextBioi 979890.

Gene expression databases

Genevestigatori P34072.

Family and domain databases

InterProi IPR013860. DUF1752_fun.
[Graphical view ]
Pfami PF08550. DUF1752. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing a cosmid clone of Saccharomyces cerevisiae chromosome XIV reveals 12 new open reading frames (ORFs) and an ancient duplication of six ORFs."
    Poehlmann R., Philippsen P.
    Yeast 12:391-402(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Characterization of the MKS1 gene, a new negative regulator of the Ras-cyclic AMP pathway in Saccharomyces cerevisiae."
    Matsuura A., Anraku Y.
    Mol. Gen. Genet. 238:6-16(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 127-584.
  5. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-442, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-247, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-25; SER-247 AND SER-276, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-442, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMKS1_YEAST
AccessioniPrimary (citable) accession number: P34072
Secondary accession number(s): D6W1A3, Q02533
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3