Reviewed, UniProtKB/Swiss-Prot P34068 (MFM1_SCHPO)

Names and origin

Protein names

Recommended name:
    M-factor

Gene names

Name:	mfm1
ORF Names:	SPAPB8E5.05

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	42 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	M-factor is a mating pheromone produced by M-type mating cells. All three mfm genes contribute to the production of M-factor.
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Induction	By nitrogen starvation. It is further induced by a pheromone signal. Its transcription is limited to M cells.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cell membrane Membrane
Molecular function	Pheromone
PTM	Lipoprotein Methylation Prenylation
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	mating pheromone activity Traceable author statement. Source: GeneDB_SPombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 30	30			PRO_0000021707
	Peptide	31 – 39	9	M-factor Ref.1		PRO_0000021708
	Propeptide	40 – 42	3	Removed in mature form		PRO_0000021709
Amino acid modifications
	Modified residue	39	1	Cysteine methyl ester Ref.1
	Lipidation	39	1	S-farnesyl cysteine

Sequences

	Sequence		Length	Mass (Da)
	P34068-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: C78488648EE780F5	FASTA	42	4,448
	10 20 30 40 MDSMANSVSS SSVVNAGNKP AETLNKTVKN YTPKVPYMCV IA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mating pheromones of the fission yeast Schizosaccharomyces pombe: purification and structural characterization of M-factor and isolation and analysis of two genes encoding the pheromone." Davey J. EMBO J. 11:951-960(1992) [PubMed: 1547790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 31-39, ISOPRENYLATION AT CYS-39, METHYLATION AT CYS-39.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X63627 Genomic DNA. Translation: CAA45175.1. CU329670 Genomic DNA. Translation: CAC37424.1.
PIR	S21027.
RefSeq	NP_594779.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P34068.
Genome annotation databases
GeneID	2543246.
GenomeReviews	Gene locus mfm1 in contig CU329670_GR.
KEGG	spo:SPAPB8E5.05.
NMPDR	fig|4896.1.peg.4749.
Organism-specific databases
GeneDB_Spombe	SPAPB8E5.05.
Gene expression databases
ArrayExpress	P34068.
Family and domain databases
InterPro	IPR005555. M-factor. [Graphical view]
Pfam	PF03855. M-factor. 1 hit. [Graphical view]
ProDom	PD018891. M-factor. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

MFM1_SCHPO

Accession

Primary (citable) accession number: P34068

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	November 24, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)