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P34067

- PSB4_RAT

UniProt

P34067 - PSB4_RAT

Protein

Proteasome subunit beta type-4

Gene

Psmb4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 2 (15 Dec 1998)
      Previous versions | rss
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    Functioni

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1 By similarity.By similarity

    Catalytic activityi

    Cleavage of peptide bonds with very broad specificity.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei45 – 451NucleophileBy similarity

    GO - Molecular functioni

    1. threonine-type endopeptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. proteolysis involved in cellular protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Threonine protease

    Protein family/group databases

    MEROPSiT01.987.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteasome subunit beta type-4 (EC:3.4.25.1)
    Alternative name(s):
    Macropain beta chain
    Multicatalytic endopeptidase complex beta chain
    Proteasome beta chain
    Proteasome chain 3
    Short name:
    RN3
    Gene namesi
    Name:Psmb4
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi61877. Psmb4.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell
    3. proteasome core complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 44441 PublicationPRO_0000026583Add
    BLAST
    Chaini45 – 263219Proteasome subunit beta type-4PRO_0000026584Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei101 – 1011PhosphotyrosineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein, Zymogen

    Proteomic databases

    PaxDbiP34067.
    PRIDEiP34067.

    2D gel databases

    World-2DPAGE0004:P34067.

    PTM databases

    PhosphoSiteiP34067.

    Expressioni

    Inductioni

    Up-regulated in cardiac hypertrophy and hypoxemia.1 Publication

    Gene expression databases

    GenevestigatoriP34067.

    Interactioni

    Subunit structurei

    The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Forms a ternary complex with SMAD1 and OAZ1 before PSMB4 is incorporated into the 20S proteasome By similarity.By similarity

    Protein-protein interaction databases

    IntActiP34067. 1 interaction.
    STRINGi10116.ENSRNOP00000028484.

    Structurei

    3D structure databases

    ProteinModelPortaliP34067.
    SMRiP34067. Positions 45-261.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase T1B family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0638.
    HOGENOMiHOG000181719.
    HOVERGENiHBG018194.
    InParanoidiP34067.
    KOiK02736.
    PhylomeDBiP34067.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    InterProiIPR029055. Ntn_hydrolases_N.
    IPR016050. Proteasome_bsu_CS.
    IPR016295. Proteasome_endopept_cplx_B.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view]
    PfamiPF00227. Proteasome. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001213. Psome_endopept_beta. 1 hit.
    SUPFAMiSSF56235. SSF56235. 1 hit.
    PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
    PS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P34067-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEAFWESRTG HWAGGPAPGQ FYRVPSTPSC LMDPMSAPAR PITRTQNPMV    50
    TGTSVLGVKF DCGVVIAADM LGSYGSLARF RNISRIMRVN DSTMLGASGD 100
    YADFQYLKQV LGQMVIDEEL FGDGHSYSPR AIHSWLTRAM YSRRSKMNPL 150
    WNTKVIGGYA GGESFLGYVD MLGVAYEAPS LATGYGAYLA QPLLREVLEK 200
    QPVLSQTEAR ELVERCMRVL YYRDARSYNR FQVATVTEKG VEIEGPLSAQ 250
    TNWDIAHMIS GFE 263
    Length:263
    Mass (Da):29,197
    Last modified:December 15, 1998 - v2
    Checksum:iC8FCB91265F8FB90
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti41 – 411P → S in AAA42054. (PubMed:8482379)Curated
    Sequence conflicti50 – 501V → L in AAA42054. (PubMed:8482379)Curated
    Sequence conflicti60 – 601F → S in AAB47113. (PubMed:8645151)Curated
    Sequence conflicti80 – 801F → L in AAB47113. (PubMed:8645151)Curated
    Sequence conflicti82 – 821N → I in AAA42054. (PubMed:8482379)Curated
    Sequence conflicti86 – 861I → F in AAB47113. (PubMed:8645151)Curated
    Sequence conflicti89 – 891V → L in AAB47113. (PubMed:8645151)Curated
    Sequence conflicti121 – 1211F → L AA sequence 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S82190 mRNA. Translation: AAB47113.2.
    L17127 mRNA. Translation: AAA42054.1.
    PIRiS09084.
    S32507.
    RefSeqiNP_113817.1. NM_031629.1.
    UniGeneiRn.6169.

    Genome annotation databases

    GeneIDi58854.
    KEGGirno:58854.
    UCSCiRGD:61877. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S82190 mRNA. Translation: AAB47113.2 .
    L17127 mRNA. Translation: AAA42054.1 .
    PIRi S09084.
    S32507.
    RefSeqi NP_113817.1. NM_031629.1.
    UniGenei Rn.6169.

    3D structure databases

    ProteinModelPortali P34067.
    SMRi P34067. Positions 45-261.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P34067. 1 interaction.
    STRINGi 10116.ENSRNOP00000028484.

    Protein family/group databases

    MEROPSi T01.987.

    PTM databases

    PhosphoSitei P34067.

    2D gel databases

    World-2DPAGE 0004:P34067.

    Proteomic databases

    PaxDbi P34067.
    PRIDEi P34067.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 58854.
    KEGGi rno:58854.
    UCSCi RGD:61877. rat.

    Organism-specific databases

    CTDi 5692.
    RGDi 61877. Psmb4.

    Phylogenomic databases

    eggNOGi COG0638.
    HOGENOMi HOG000181719.
    HOVERGENi HBG018194.
    InParanoidi P34067.
    KOi K02736.
    PhylomeDBi P34067.

    Miscellaneous databases

    NextBioi 611445.
    PROi P34067.

    Gene expression databases

    Genevestigatori P34067.

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    InterProi IPR029055. Ntn_hydrolases_N.
    IPR016050. Proteasome_bsu_CS.
    IPR016295. Proteasome_endopept_cplx_B.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view ]
    Pfami PF00227. Proteasome. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001213. Psome_endopept_beta. 1 hit.
    SUPFAMi SSF56235. SSF56235. 1 hit.
    PROSITEi PS00854. PROTEASOME_BETA_1. 1 hit.
    PS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Processing of N3, a mammalian proteasome beta-type subunit."
      Thomson S., Rivett A.J.
      Biochem. J. 315:733-738(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-140.
    2. "cDNA cloning of a new type of subunit of mammalian proteasomes."
      Thomson S., Balson D.F., Rivett A.J.
      FEBS Lett. 322:135-138(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 32-263.
    3. "N-terminal sequence similarities between components of the multicatalytic proteinase complex."
      Lilley K.S., Davison M.D., Rivett A.J.
      FEBS Lett. 262:327-329(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 45-55.
    4. Lubec G., Diao W.
      Submitted (NOV-2006) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 60-79 AND 109-130, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Hippocampus.
    5. "Atrophy, hypertrophy, and hypoxemia induce transcriptional regulators of the ubiquitin proteasome system in the rat heart."
      Razeghi P., Baskin K.K., Sharma S., Young M.E., Stepkowski S., Essop M.F., Taegtmeyer H.
      Biochem. Biophys. Res. Commun. 342:361-364(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.

    Entry informationi

    Entry nameiPSB4_RAT
    AccessioniPrimary (citable) accession number: P34067
    Secondary accession number(s): P28071, P97719
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: December 15, 1998
    Last modified: October 1, 2014
    This is version 120 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3