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P34067 (PSB4_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proteasome subunit beta type-4

EC=3.4.25.1
Alternative name(s):
Macropain beta chain
Multicatalytic endopeptidase complex beta chain
Proteasome beta chain
Proteasome chain 3
Short name=RN3
Gene names
Name:Psmb4
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length263 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1 By similarity.

Catalytic activity

Cleavage of peptide bonds with very broad specificity.

Subunit structure

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Forms a ternary complex with SMAD1 and OAZ1 before PSMB4 is incorporated into the 20S proteasome By similarity.

Subcellular location

Cytoplasm. Nucleus.

Induction

Up-regulated in cardiac hypertrophy and hypoxemia. Ref.5

Sequence similarities

Belongs to the peptidase T1B family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 4444
PRO_0000026583
Chain45 – 263219Proteasome subunit beta type-4
PRO_0000026584

Sites

Active site451Nucleophile By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue1011Phosphotyrosine By similarity

Experimental info

Sequence conflict411P → S in AAA42054. Ref.2
Sequence conflict501V → L in AAA42054. Ref.2
Sequence conflict601F → S in AAB47113. Ref.1
Sequence conflict801F → L in AAB47113. Ref.1
Sequence conflict821N → I in AAA42054. Ref.2
Sequence conflict861I → F in AAB47113. Ref.1
Sequence conflict891V → L in AAB47113. Ref.1
Sequence conflict1211F → L AA sequence Ref.4

Sequences

Sequence LengthMass (Da)Tools
P34067 [UniParc].

Last modified December 15, 1998. Version 2.
Checksum: C8FCB91265F8FB90

FASTA26329,197
        10         20         30         40         50         60 
MEAFWESRTG HWAGGPAPGQ FYRVPSTPSC LMDPMSAPAR PITRTQNPMV TGTSVLGVKF 

        70         80         90        100        110        120 
DCGVVIAADM LGSYGSLARF RNISRIMRVN DSTMLGASGD YADFQYLKQV LGQMVIDEEL 

       130        140        150        160        170        180 
FGDGHSYSPR AIHSWLTRAM YSRRSKMNPL WNTKVIGGYA GGESFLGYVD MLGVAYEAPS 

       190        200        210        220        230        240 
LATGYGAYLA QPLLREVLEK QPVLSQTEAR ELVERCMRVL YYRDARSYNR FQVATVTEKG 

       250        260 
VEIEGPLSAQ TNWDIAHMIS GFE 

« Hide

References

[1]"Processing of N3, a mammalian proteasome beta-type subunit."
Thomson S., Rivett A.J.
Biochem. J. 315:733-738(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-140.
[2]"cDNA cloning of a new type of subunit of mammalian proteasomes."
Thomson S., Balson D.F., Rivett A.J.
FEBS Lett. 322:135-138(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 32-263.
[3]"N-terminal sequence similarities between components of the multicatalytic proteinase complex."
Lilley K.S., Davison M.D., Rivett A.J.
FEBS Lett. 262:327-329(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 45-55.
[4]Lubec G., Diao W.
Submitted (NOV-2006) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 60-79 AND 109-130, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Hippocampus.
[5]"Atrophy, hypertrophy, and hypoxemia induce transcriptional regulators of the ubiquitin proteasome system in the rat heart."
Razeghi P., Baskin K.K., Sharma S., Young M.E., Stepkowski S., Essop M.F., Taegtmeyer H.
Biochem. Biophys. Res. Commun. 342:361-364(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S82190 mRNA. Translation: AAB47113.2.
L17127 mRNA. Translation: AAA42054.1.
PIRS09084.
S32507.
RefSeqNP_113817.1. NM_031629.1.
UniGeneRn.6169.

3D structure databases

ProteinModelPortalP34067.
SMRP34067. Positions 45-261.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP34067. 1 interaction.
STRING10116.ENSRNOP00000028484.

Protein family/group databases

MEROPST01.987.

PTM databases

PhosphoSiteP34067.

2D gel databases

World-2DPAGE0004:P34067.

Proteomic databases

PaxDbP34067.
PRIDEP34067.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID58854.
KEGGrno:58854.
UCSCRGD:61877. rat.

Organism-specific databases

CTD5692.
RGD61877. Psmb4.

Phylogenomic databases

eggNOGCOG0638.
HOGENOMHOG000181719.
HOVERGENHBG018194.
InParanoidP34067.
KOK02736.
PhylomeDBP34067.

Gene expression databases

GenevestigatorP34067.

Family and domain databases

Gene3D3.60.20.10. 1 hit.
InterProIPR029055. Ntn_hydrolases_N.
IPR016050. Proteasome_bsu_CS.
IPR016295. Proteasome_endopept_cplx_B.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamPF00227. Proteasome. 1 hit.
[Graphical view]
PIRSFPIRSF001213. Psome_endopept_beta. 1 hit.
SUPFAMSSF56235. SSF56235. 1 hit.
PROSITEPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio611445.
PROP34067.

Entry information

Entry namePSB4_RAT
AccessionPrimary (citable) accession number: P34067
Secondary accession number(s): P28071, P97719
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: December 15, 1998
Last modified: June 11, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries