Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P34067

- PSB4_RAT

UniProt

P34067 - PSB4_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Proteasome subunit beta type-4

Gene

Psmb4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1 By similarity.By similarity

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei45 – 451NucleophileBy similarity

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. proteolysis involved in cellular protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-4 (EC:3.4.25.1)
Alternative name(s):
Macropain beta chain
Multicatalytic endopeptidase complex beta chain
Proteasome beta chain
Proteasome chain 3
Short name:
RN3
Gene namesi
Name:Psmb4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi61877. Psmb4.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
  3. proteasome core complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 44441 PublicationPRO_0000026583Add
BLAST
Chaini45 – 263219Proteasome subunit beta type-4PRO_0000026584Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei101 – 1011PhosphotyrosineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Zymogen

Proteomic databases

PaxDbiP34067.
PRIDEiP34067.

2D gel databases

World-2DPAGE0004:P34067.

PTM databases

PhosphoSiteiP34067.

Expressioni

Inductioni

Up-regulated in cardiac hypertrophy and hypoxemia.1 Publication

Gene expression databases

GenevestigatoriP34067.

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Forms a ternary complex with SMAD1 and OAZ1 before PSMB4 is incorporated into the 20S proteasome By similarity.By similarity

Protein-protein interaction databases

IntActiP34067. 1 interaction.
STRINGi10116.ENSRNOP00000028484.

Structurei

3D structure databases

ProteinModelPortaliP34067.
SMRiP34067. Positions 45-261.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0638.
HOGENOMiHOG000181719.
HOVERGENiHBG018194.
InParanoidiP34067.
KOiK02736.
PhylomeDBiP34067.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR016050. Proteasome_bsu_CS.
IPR016295. Proteasome_endopept_cplx_B.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PIRSFiPIRSF001213. Psome_endopept_beta. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P34067-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEAFWESRTG HWAGGPAPGQ FYRVPSTPSC LMDPMSAPAR PITRTQNPMV
60 70 80 90 100
TGTSVLGVKF DCGVVIAADM LGSYGSLARF RNISRIMRVN DSTMLGASGD
110 120 130 140 150
YADFQYLKQV LGQMVIDEEL FGDGHSYSPR AIHSWLTRAM YSRRSKMNPL
160 170 180 190 200
WNTKVIGGYA GGESFLGYVD MLGVAYEAPS LATGYGAYLA QPLLREVLEK
210 220 230 240 250
QPVLSQTEAR ELVERCMRVL YYRDARSYNR FQVATVTEKG VEIEGPLSAQ
260
TNWDIAHMIS GFE
Length:263
Mass (Da):29,197
Last modified:December 15, 1998 - v2
Checksum:iC8FCB91265F8FB90
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti41 – 411P → S in AAA42054. (PubMed:8482379)Curated
Sequence conflicti50 – 501V → L in AAA42054. (PubMed:8482379)Curated
Sequence conflicti60 – 601F → S in AAB47113. (PubMed:8645151)Curated
Sequence conflicti80 – 801F → L in AAB47113. (PubMed:8645151)Curated
Sequence conflicti82 – 821N → I in AAA42054. (PubMed:8482379)Curated
Sequence conflicti86 – 861I → F in AAB47113. (PubMed:8645151)Curated
Sequence conflicti89 – 891V → L in AAB47113. (PubMed:8645151)Curated
Sequence conflicti121 – 1211F → L AA sequence 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S82190 mRNA. Translation: AAB47113.2.
L17127 mRNA. Translation: AAA42054.1.
PIRiS09084.
S32507.
RefSeqiNP_113817.1. NM_031629.1.
UniGeneiRn.6169.

Genome annotation databases

GeneIDi58854.
KEGGirno:58854.
UCSCiRGD:61877. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S82190 mRNA. Translation: AAB47113.2 .
L17127 mRNA. Translation: AAA42054.1 .
PIRi S09084.
S32507.
RefSeqi NP_113817.1. NM_031629.1.
UniGenei Rn.6169.

3D structure databases

ProteinModelPortali P34067.
SMRi P34067. Positions 45-261.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P34067. 1 interaction.
STRINGi 10116.ENSRNOP00000028484.

PTM databases

PhosphoSitei P34067.

2D gel databases

World-2DPAGE 0004:P34067.

Proteomic databases

PaxDbi P34067.
PRIDEi P34067.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 58854.
KEGGi rno:58854.
UCSCi RGD:61877. rat.

Organism-specific databases

CTDi 5692.
RGDi 61877. Psmb4.

Phylogenomic databases

eggNOGi COG0638.
HOGENOMi HOG000181719.
HOVERGENi HBG018194.
InParanoidi P34067.
KOi K02736.
PhylomeDBi P34067.

Miscellaneous databases

NextBioi 611445.
PROi P34067.

Gene expression databases

Genevestigatori P34067.

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
InterProi IPR029055. Ntn_hydrolases_N.
IPR016050. Proteasome_bsu_CS.
IPR016295. Proteasome_endopept_cplx_B.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view ]
Pfami PF00227. Proteasome. 1 hit.
[Graphical view ]
PIRSFi PIRSF001213. Psome_endopept_beta. 1 hit.
SUPFAMi SSF56235. SSF56235. 1 hit.
PROSITEi PS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Processing of N3, a mammalian proteasome beta-type subunit."
    Thomson S., Rivett A.J.
    Biochem. J. 315:733-738(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-140.
  2. "cDNA cloning of a new type of subunit of mammalian proteasomes."
    Thomson S., Balson D.F., Rivett A.J.
    FEBS Lett. 322:135-138(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 32-263.
  3. "N-terminal sequence similarities between components of the multicatalytic proteinase complex."
    Lilley K.S., Davison M.D., Rivett A.J.
    FEBS Lett. 262:327-329(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 45-55.
  4. Lubec G., Diao W.
    Submitted (NOV-2006) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 60-79 AND 109-130, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus.
  5. "Atrophy, hypertrophy, and hypoxemia induce transcriptional regulators of the ubiquitin proteasome system in the rat heart."
    Razeghi P., Baskin K.K., Sharma S., Young M.E., Stepkowski S., Essop M.F., Taegtmeyer H.
    Biochem. Biophys. Res. Commun. 342:361-364(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiPSB4_RAT
AccessioniPrimary (citable) accession number: P34067
Secondary accession number(s): P28071, P97719
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: December 15, 1998
Last modified: October 29, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3