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P34066 (PSA1A_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proteasome subunit alpha type-1-A

EC=3.4.25.1
Alternative name(s):
20S proteasome alpha subunit F-1
Proteasome 30 kDa subunit
Proteasome component 2A
Short name=AtPSM30
Proteasome subunit alpha type-6
Protein ARSENIC TOLERANCE 5
Gene names
Name:PAF1
Synonyms:ARS5, PRC2A, PRS1, PSM30
Ordered Locus Names:At5g42790
ORF Names:MJB21.17
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length278 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Negatively regulates thiol biosynthesis and arsenic tolerance. Ref.10

Catalytic activity

Cleavage of peptide bonds with very broad specificity.

Subunit structure

Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Ref.8 Ref.9 Ref.11

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Developmental stage

Levels decrease as seedling grow (9-day-old) but is high in 3-week-old plants. Ref.1

Induction

Slightly induced by heat-shock and high intensity light. Ref.1

Disruption phenotype

Enhanced thiol accumulation and arsenic tolerance. Mutation can be complemented by overexpression of PAF2. Ref.10

Sequence similarities

Belongs to the peptidase T1A family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 278278Proteasome subunit alpha type-1-A
PRO_0000124072

Amino acid modifications

Modified residue11N-acetylmethionine Ref.11
Cross-link51Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.11

Experimental info

Sequence conflict2441G → A in AAM61575. Ref.6
Sequence conflict2571E → G in AAC32062. Ref.2
Sequence conflict2571E → G in AAM61575. Ref.6
Sequence conflict2631R → Q in AAC32062. Ref.2
Sequence conflict2631R → Q in AAM61575. Ref.6
Sequence conflict2671V → G in AAC32062. Ref.2
Sequence conflict2671V → G in AAM61575. Ref.6

Sequences

Sequence LengthMass (Da)Tools
P34066 [UniParc].

Last modified November 2, 2001. Version 3.
Checksum: 93E1BC4D0EA99F70

FASTA27830,476
        10         20         30         40         50         60 
MFRNQYDTDV TTWSPTGRLF QVEYAMEAVK QGSAAIGLRS RSHVVLACVN KAQSELSSHQ 

        70         80         90        100        110        120 
RKIFKVDDHI GVAIAGLTAD GRVLSRYMRS ESINHSFTYE SPLPVGRLVV HLADKAQVCT 

       130        140        150        160        170        180 
QRSWKRPYGV GLLVGGLDES GAHLYYNCPS GNYFEYQAFA IGSRSQAAKT YLERRFESFG 

       190        200        210        220        230        240 
DSSREDLIKD AILAVRETLQ GETLKSSLCT VAILGVDEPF HFLDQEAIQK VIDTFEKVPE 

       250        260        270 
EEEGEGEAGE GEAEAAEAAP AERGGGVAGD QDVAPMEM 

« Hide

References

« Hide 'large scale' references
[1]"An Arabidopsis gene homologous to mammalian and insect genes encoding the largest proteasome subunit."
Shirley B.W., Goodman H.M.
Mol. Gen. Genet. 241:586-594(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, DEVELOPMENTAL STAGE.
Strain: cv. Landsberg erecta.
[2]"Molecular organization of the 20S proteasome gene family from Arabidopsis thaliana."
Fu H., Doelling J.H., Arendt C.S., Hochstrasser M., Vierstra R.D.
Genetics 149:677-692(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE.
Strain: cv. Columbia.
[3]"Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]"The 20S proteasome gene family in Arabidopsis thaliana."
Parmentier Y., Bouchez D., Fleck J., Genschik P.
FEBS Lett. 416:281-285(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[8]"Structure and functional analyses of the 26S proteasome subunits from plants."
Fu H., Girod P.-A., Doelling J.H., van Nocker S., Hochstrasser M., Finley D., Vierstra R.D.
Mol. Biol. Rep. 26:137-146(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT.
[9]"Purification of the Arabidopsis 26 S proteasome: biochemical and molecular analyses revealed the presence of multiple isoforms."
Yang P., Fu H., Walker J., Papa C.M., Smalle J., Ju Y.-M., Vierstra R.D.
J. Biol. Chem. 279:6401-6413(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY.
[10]"ARS5 is a component of the 26S proteasome complex, and negatively regulates thiol biosynthesis and arsenic tolerance in Arabidopsis."
Sung D.-Y., Kim T.-H., Komives E.A., Mendoza-Cozatl D.G., Schroeder J.I.
Plant J. 59:802-812(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[11]"Affinity purification of the Arabidopsis 26 S proteasome reveals a diverse array of plant proteolytic complexes."
Book A.J., Gladman N.P., Lee S.S., Scalf M., Smith L.M., Vierstra R.D.
J. Biol. Chem. 285:25554-25569(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, CHARACTERIZATION OF THE 26S PROTEASOME COMPLEX, SUBUNIT, ACETYLATION AT MET-1, UBIQUITINATION AT LYS-51.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M98495 Unassigned DNA. Translation: AAA16326.1.
AF043526 mRNA. Translation: AAC32062.1.
AB007647 Genomic DNA. Translation: BAB10635.1.
CP002688 Genomic DNA. Translation: AED94864.1.
AF375447 mRNA. Translation: AAK53031.1.
AY058128 mRNA. Translation: AAL25544.1.
AY113047 mRNA. Translation: AAM47355.1.
AY085017 mRNA. Translation: AAM61575.1.
PIRS39900.
T51974.
RefSeqNP_199093.1. NM_123644.4.
UniGeneAt.23724.

3D structure databases

ProteinModelPortalP34066.
SMRP34066. Positions 2-232.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid19540. 1 interaction.
IntActP34066. 1 interaction.
STRING3702.AT5G42790.1-P.

Protein family/group databases

MEROPST01.976.

Proteomic databases

PaxDbP34066.
PRIDEP34066.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G42790.1; AT5G42790.1; AT5G42790.
GeneID834290.
KEGGath:AT5G42790.

Organism-specific databases

TAIRAT5G42790.

Phylogenomic databases

eggNOGCOG0638.
HOGENOMHOG000091080.
InParanoidP34066.
KOK02725.
OMAENAEMFR.
PhylomeDBP34066.

Enzyme and pathway databases

BioCycARA:AT5G42790-MONOMER.

Gene expression databases

GenevestigatorP34066.

Family and domain databases

Gene3D3.60.20.10. 1 hit.
InterProIPR029055. Ntn_hydrolases_N.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMSSF56235. SSF56235. 1 hit.
PROSITEPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP34066.

Entry information

Entry namePSA1A_ARATH
AccessionPrimary (citable) accession number: P34066
Secondary accession number(s): O81150, Q8LF69
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 2, 2001
Last modified: June 11, 2014
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names