Reviewed, UniProtKB/Swiss-Prot P34066 (PSA1A_ARATH)

Names and origin

Protein names

Recommended name:
    Proteasome subunit alpha type-1-A
    EC=3.4.25.1
Alternative name(s):
    Proteasome subunit alpha type-6
    20S proteasome alpha subunit F-1
    Proteasome 30 kDa subunit
    Proteasome component 2A
      Short name=AtPSM30
    Protein ARSENIC TOLERANCE 5

Gene names

Name:	PAF1
Synonyms:	ARS5, PRC2A, PRS1, PSM30
Ordered Locus Names:	At5g42790
ORF Names:	MJB21.17

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Negatively regulates thiol biosynthesis and arsenic tolerance.
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Developmental stage	Levels decrease as seedling grow (9-day-old) but is high in 3-week-old plants. Ref.1
Induction	Slightly induced by heat-shock and high intensity light. Ref.1
Disruption phenotype	Enhanced thiol accumulation and arsenic tolerance. Mutation can be complemented by over-expression of PAF2.
Sequence similarities	Belongs to the peptidase T1A family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Threonine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	response to arsenic Ref.9 Inferred from mutant phenotype. Source: TAIR ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: TAIR proteasome core complex, alpha-subunit complex Inferred from electronic annotation. Source: InterPro
Molecular function	threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	Proteasome subunit alpha type-1-A		PRO_0000124072
Experimental info
	Sequence conflict	244	1	G → A in AAM61575. Ref.5
	Sequence conflict	257	1	E → G Ref.2
	Sequence conflict	257	1	E → G Ref.5
	Sequence conflict	263	1	R → Q Ref.2
	Sequence conflict	263	1	R → Q Ref.5
	Sequence conflict	267	1	V → G Ref.2
	Sequence conflict	267	1	V → G Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P34066-1 [UniParc]. Last modified November 2, 2001. Version 3. Checksum: 93E1BC4D0EA99F70	FASTA	278	30,476
	10 20 30 40 50 60 MFRNQYDTDV TTWSPTGRLF QVEYAMEAVK QGSAAIGLRS RSHVVLACVN KAQSELSSHQ 70 80 90 100 110 120 RKIFKVDDHI GVAIAGLTAD GRVLSRYMRS ESINHSFTYE SPLPVGRLVV HLADKAQVCT 130 140 150 160 170 180 QRSWKRPYGV GLLVGGLDES GAHLYYNCPS GNYFEYQAFA IGSRSQAAKT YLERRFESFG 190 200 210 220 230 240 DSSREDLIKD AILAVRETLQ GETLKSSLCT VAILGVDEPF HFLDQEAIQK VIDTFEKVPE 250 260 270 EEEGEGEAGE GEAEAAEAAP AERGGGVAGD QDVAPMEM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An Arabidopsis gene homologous to mammalian and insect genes encoding the largest proteasome subunit." Shirley B.W., Goodman H.M. Mol. Gen. Genet. 241:586-594(1993) [PubMed: 8264533] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, DEVELOPMENTAL STAGE. Strain: cv. Landsberg erecta.
[2]	"Molecular organization of the 20S proteasome gene family from Arabidopsis thaliana." Fu H., Doelling J.H., Arendt C.S., Hochstrasser M., Vierstra R.D. Genetics 149:677-692(1998) [PubMed: 9611183] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE. Strain: cv. Columbia.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones." Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S. DNA Res. 4:401-414(1997) [PubMed: 9501997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The 20S proteasome gene family in Arabidopsis thaliana." Parmentier Y., Bouchez D., Fleck J., Genschik P. FEBS Lett. 416:281-285(1997) [PubMed: 9373170] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[7]	"Structure and functional analyses of the 26S proteasome subunits from plants." Fu H., Girod P.-A., Doelling J.H., van Nocker S., Hochstrasser M., Finley D., Vierstra R.D. Mol. Biol. Rep. 26:137-146(1999) [PubMed: 10363660] [Abstract] Cited for: SUBUNIT.
[8]	"Purification of the Arabidopsis 26 S proteasome: biochemical and molecular analyses revealed the presence of multiple isoforms." Yang P., Fu H., Walker J., Papa C.M., Smalle J., Ju Y.-M., Vierstra R.D. J. Biol. Chem. 279:6401-6413(2004) [PubMed: 14623884] [Abstract] Cited for: SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY.
[9]	"ARS5 is a component of the 26S proteasome complex, and negatively regulates thiol biosynthesis and arsenic tolerance in Arabidopsis." Sung D.-Y., Kim T.-H., Komives E.A., Mendoza-Cozatl D.G., Schroeder J.I. Plant J. 59:802-812(2009) [PubMed: 19453443] [Abstract] Cited for: FUNCTION AND DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M98495 Unassigned DNA. Translation: AAA16326.1. AF043526 mRNA. Translation: AAC32062.1. AB007647 Genomic DNA. Translation: BAB10635.1. AF375447 mRNA. Translation: AAK53031.1. AY058128 mRNA. Translation: AAL25544.1. AY113047 mRNA. Translation: AAM47355.1. AY085017 mRNA. Translation: AAM61575.1.
IPI	IPI00525551.
PIR	S39900. T51974.
RefSeq	NP_199093.1.
UniGene	At.23724
3D structure databases
SMR	P34066. Positions 2-232.
ModBase	Search...
Protein-protein interaction databases
STRING	P34066.
Proteomic databases
PRIDE	P34066.
Genome annotation databases
GeneID	834290.
GenomeReviews	Gene locus AT5G42790 in contig BA000015_GR.
KEGG	ath:AT5G42790.
NMPDR	fig|3702.1.peg.26004.
Organism-specific databases
TAIR	At5g42790.
Phylogenomic databases
eggNOG	KOG0863.
HOGENOM	HBG499923.
InParanoid	P34066.
OMA	MFRNSYD.
PhylomeDB	P34066.
Enzyme and pathway databases
BRENDA	3.4.25.1. 302.
Gene expression databases
ArrayExpress	P34066.
Genevestigator	P34066.
GermOnline	AT5G42790. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000426. Proteasome_asu_CS. IPR001353. Proteasome_sua/b. [Graphical view]
Pfam	PF00227. Proteasome. 1 hit. PF10584. Proteasome_A_N. 1 hit. [Graphical view]
PROSITE	PS00388. PROTEASOME_A_1. 1 hit. PS51475. PROTEASOME_A_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSA1A_ARATH

Accession

Primary (citable) accession number: P34066
Secondary accession number(s): O81150, Q8LF69

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	November 2, 2001
Last modified:	February 9, 2010
This is version 89 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)