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P34066

- PSA1A_ARATH

UniProt

P34066 - PSA1A_ARATH

Protein

Proteasome subunit alpha type-1-A

Gene

PAF1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 3 (02 Nov 2001)
      Previous versions | rss
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    Functioni

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Negatively regulates thiol biosynthesis and arsenic tolerance.1 Publication

    Catalytic activityi

    Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

    GO - Molecular functioni

    1. threonine-type endopeptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. protein catabolic process Source: TAIR
    2. response to arsenic-containing substance Source: TAIR
    3. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Threonine protease

    Enzyme and pathway databases

    BioCyciARA:AT5G42790-MONOMER.
    ReactomeiREACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_107429. Orc1 removal from chromatin.
    REACT_185297. Separation of Sister Chromatids.
    REACT_187712. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_187719. ER-Phagosome pathway.
    REACT_187848. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_190947. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_94347. APC/C:Cdc20 mediated degradation of Securin.

    Protein family/group databases

    MEROPSiT01.976.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteasome subunit alpha type-1-A (EC:3.4.25.1)
    Alternative name(s):
    20S proteasome alpha subunit F-1
    Proteasome 30 kDa subunit
    Proteasome component 2A
    Short name:
    AtPSM30
    Proteasome subunit alpha type-6
    Protein ARSENIC TOLERANCE 5
    Gene namesi
    Name:PAF1
    Synonyms:ARS5, PRC2A, PRS1, PSM30
    Ordered Locus Names:At5g42790
    ORF Names:MJB21.17
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G42790.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. cytosol Source: TAIR
    2. nucleus Source: UniProtKB-SubCell
    3. plasma membrane Source: TAIR
    4. proteasome complex Source: TAIR
    5. proteasome core complex, alpha-subunit complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Proteasome

    Pathology & Biotechi

    Disruption phenotypei

    Enhanced thiol accumulation and arsenic tolerance. Mutation can be complemented by overexpression of PAF2.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 278278Proteasome subunit alpha type-1-APRO_0000124072Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication
    Cross-linki51 – 51Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

    Keywords - PTMi

    Acetylation, Isopeptide bond, Ubl conjugation

    Proteomic databases

    PaxDbiP34066.
    PRIDEiP34066.

    Expressioni

    Developmental stagei

    Levels decrease as seedling grow (9-day-old) but is high in 3-week-old plants.1 Publication

    Inductioni

    Slightly induced by heat-shock and high intensity light.1 Publication

    Gene expression databases

    GenevestigatoriP34066.

    Interactioni

    Subunit structurei

    Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.3 Publications

    Protein-protein interaction databases

    BioGridi19540. 1 interaction.
    IntActiP34066. 1 interaction.
    STRINGi3702.AT5G42790.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliP34066.
    SMRiP34066. Positions 2-232.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase T1A family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0638.
    HOGENOMiHOG000091080.
    InParanoidiP34066.
    KOiK02725.
    OMAiENAEMFR.
    PhylomeDBiP34066.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    InterProiIPR029055. Ntn_hydrolases_N.
    IPR000426. Proteasome_asu_N.
    IPR023332. Proteasome_suA-type.
    IPR001353. Proteasome_sua/b.
    [Graphical view]
    PfamiPF00227. Proteasome. 1 hit.
    PF10584. Proteasome_A_N. 1 hit.
    [Graphical view]
    SMARTiSM00948. Proteasome_A_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56235. SSF56235. 1 hit.
    PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
    PS51475. PROTEASOME_ALPHA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P34066-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFRNQYDTDV TTWSPTGRLF QVEYAMEAVK QGSAAIGLRS RSHVVLACVN    50
    KAQSELSSHQ RKIFKVDDHI GVAIAGLTAD GRVLSRYMRS ESINHSFTYE 100
    SPLPVGRLVV HLADKAQVCT QRSWKRPYGV GLLVGGLDES GAHLYYNCPS 150
    GNYFEYQAFA IGSRSQAAKT YLERRFESFG DSSREDLIKD AILAVRETLQ 200
    GETLKSSLCT VAILGVDEPF HFLDQEAIQK VIDTFEKVPE EEEGEGEAGE 250
    GEAEAAEAAP AERGGGVAGD QDVAPMEM 278
    Length:278
    Mass (Da):30,476
    Last modified:November 2, 2001 - v3
    Checksum:i93E1BC4D0EA99F70
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti244 – 2441G → A in AAM61575. 1 PublicationCurated
    Sequence conflicti257 – 2571E → G in AAC32062. (PubMed:9611183)Curated
    Sequence conflicti257 – 2571E → G in AAM61575. 1 PublicationCurated
    Sequence conflicti263 – 2631R → Q in AAC32062. (PubMed:9611183)Curated
    Sequence conflicti263 – 2631R → Q in AAM61575. 1 PublicationCurated
    Sequence conflicti267 – 2671V → G in AAC32062. (PubMed:9611183)Curated
    Sequence conflicti267 – 2671V → G in AAM61575. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M98495 Unassigned DNA. Translation: AAA16326.1.
    AF043526 mRNA. Translation: AAC32062.1.
    AB007647 Genomic DNA. Translation: BAB10635.1.
    CP002688 Genomic DNA. Translation: AED94864.1.
    AF375447 mRNA. Translation: AAK53031.1.
    AY058128 mRNA. Translation: AAL25544.1.
    AY113047 mRNA. Translation: AAM47355.1.
    AY085017 mRNA. Translation: AAM61575.1.
    PIRiS39900.
    T51974.
    RefSeqiNP_199093.1. NM_123644.4.
    UniGeneiAt.23724.

    Genome annotation databases

    EnsemblPlantsiAT5G42790.1; AT5G42790.1; AT5G42790.
    GeneIDi834290.
    KEGGiath:AT5G42790.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M98495 Unassigned DNA. Translation: AAA16326.1 .
    AF043526 mRNA. Translation: AAC32062.1 .
    AB007647 Genomic DNA. Translation: BAB10635.1 .
    CP002688 Genomic DNA. Translation: AED94864.1 .
    AF375447 mRNA. Translation: AAK53031.1 .
    AY058128 mRNA. Translation: AAL25544.1 .
    AY113047 mRNA. Translation: AAM47355.1 .
    AY085017 mRNA. Translation: AAM61575.1 .
    PIRi S39900.
    T51974.
    RefSeqi NP_199093.1. NM_123644.4.
    UniGenei At.23724.

    3D structure databases

    ProteinModelPortali P34066.
    SMRi P34066. Positions 2-232.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 19540. 1 interaction.
    IntActi P34066. 1 interaction.
    STRINGi 3702.AT5G42790.1-P.

    Protein family/group databases

    MEROPSi T01.976.

    Proteomic databases

    PaxDbi P34066.
    PRIDEi P34066.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G42790.1 ; AT5G42790.1 ; AT5G42790 .
    GeneIDi 834290.
    KEGGi ath:AT5G42790.

    Organism-specific databases

    TAIRi AT5G42790.

    Phylogenomic databases

    eggNOGi COG0638.
    HOGENOMi HOG000091080.
    InParanoidi P34066.
    KOi K02725.
    OMAi ENAEMFR.
    PhylomeDBi P34066.

    Enzyme and pathway databases

    BioCyci ARA:AT5G42790-MONOMER.
    Reactomei REACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_107429. Orc1 removal from chromatin.
    REACT_185297. Separation of Sister Chromatids.
    REACT_187712. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_187719. ER-Phagosome pathway.
    REACT_187848. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_190947. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_94347. APC/C:Cdc20 mediated degradation of Securin.

    Miscellaneous databases

    PROi P34066.

    Gene expression databases

    Genevestigatori P34066.

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    InterProi IPR029055. Ntn_hydrolases_N.
    IPR000426. Proteasome_asu_N.
    IPR023332. Proteasome_suA-type.
    IPR001353. Proteasome_sua/b.
    [Graphical view ]
    Pfami PF00227. Proteasome. 1 hit.
    PF10584. Proteasome_A_N. 1 hit.
    [Graphical view ]
    SMARTi SM00948. Proteasome_A_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56235. SSF56235. 1 hit.
    PROSITEi PS00388. PROTEASOME_ALPHA_1. 1 hit.
    PS51475. PROTEASOME_ALPHA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "An Arabidopsis gene homologous to mammalian and insect genes encoding the largest proteasome subunit."
      Shirley B.W., Goodman H.M.
      Mol. Gen. Genet. 241:586-594(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, DEVELOPMENTAL STAGE.
      Strain: cv. Landsberg erecta.
    2. "Molecular organization of the 20S proteasome gene family from Arabidopsis thaliana."
      Fu H., Doelling J.H., Arendt C.S., Hochstrasser M., Vierstra R.D.
      Genetics 149:677-692(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE.
      Strain: cv. Columbia.
    3. "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
      Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
      DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    7. "The 20S proteasome gene family in Arabidopsis thaliana."
      Parmentier Y., Bouchez D., Fleck J., Genschik P.
      FEBS Lett. 416:281-285(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    8. "Structure and functional analyses of the 26S proteasome subunits from plants."
      Fu H., Girod P.-A., Doelling J.H., van Nocker S., Hochstrasser M., Finley D., Vierstra R.D.
      Mol. Biol. Rep. 26:137-146(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.
    9. "Purification of the Arabidopsis 26 S proteasome: biochemical and molecular analyses revealed the presence of multiple isoforms."
      Yang P., Fu H., Walker J., Papa C.M., Smalle J., Ju Y.-M., Vierstra R.D.
      J. Biol. Chem. 279:6401-6413(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY.
    10. "ARS5 is a component of the 26S proteasome complex, and negatively regulates thiol biosynthesis and arsenic tolerance in Arabidopsis."
      Sung D.-Y., Kim T.-H., Komives E.A., Mendoza-Cozatl D.G., Schroeder J.I.
      Plant J. 59:802-812(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    11. "Affinity purification of the Arabidopsis 26 S proteasome reveals a diverse array of plant proteolytic complexes."
      Book A.J., Gladman N.P., Lee S.S., Scalf M., Smith L.M., Vierstra R.D.
      J. Biol. Chem. 285:25554-25569(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, CHARACTERIZATION OF THE 26S PROTEASOME COMPLEX, SUBUNIT, ACETYLATION AT MET-1, UBIQUITINATION AT LYS-51.

    Entry informationi

    Entry nameiPSA1A_ARATH
    AccessioniPrimary (citable) accession number: P34066
    Secondary accession number(s): O81150, Q8LF69
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: November 2, 2001
    Last modified: October 1, 2014
    This is version 124 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3