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Protein

Proteasome subunit beta type-5

Gene

PSMB5

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei56 – 561NucleophileBy similarity

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. proteolysis involved in cellular protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-5 (EC:3.4.25.1)
Alternative name(s):
Macropain chain 1
Multicatalytic endopeptidase complex chain 1
Proteasome chain 1
Proteasome subunit C1
Gene namesi
Name:PSMB5
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539 Componenti: Unplaced

Subcellular locationi

Cytoplasm PROSITE-ProRule annotation. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
  3. proteasome core complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei‹1 – 55›55Removed in mature formBy similarityPRO_0000026603Add
BLAST
Chaini56 – 256201Proteasome subunit beta type-5PRO_0000026604Add
BLAST

Keywords - PTMi

Zymogen

Proteomic databases

PRIDEiP34065.

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP34065.
SMRiP34065. Positions 56-256.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG108297.
InParanoidiP34065.
KOiK02737.
PhylomeDBiP34065.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P34065-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
CNMALADIVR LPPASEAPFA PLGAPRDLSG PPSKLAVRPW GGADLPGPGL
60 70 80 90 100
QLLHGTTTLA FKFAHGVVVA VDSRATAGSY IASQTVQKVI EINPSLLGTM
110 120 130 140 150
AGGAADCSFW ERLLARQCRV YELRNKEPIS VAAASKLLAN MVYQYKGMGL
160 170 180 190 200
SMGTMICGWD KRGPGLYYVD SEGTRIPGEA FAVGSGSSYA YGVLDGGRRP
210 220 230 240 250
DMATDEALEL ARRAIFQAAR RDAYSGGSVT VYHVGPRGWR RVSSHDVAGL

HDGYGG
Length:256
Mass (Da):27,045
Last modified:July 15, 1998 - v2
Checksum:iED467B1C925D5186
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB001935 mRNA. Translation: BAA19471.1.
X57210 mRNA. Translation: CAA40494.1.
PIRiS30539.
RefSeqiNP_001264655.1. NM_001277726.1.
UniGeneiGga.4313.
Gga.46493.
Gga.55806.

Genome annotation databases

GeneIDi396003.
KEGGigga:396003.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB001935 mRNA. Translation: BAA19471.1.
X57210 mRNA. Translation: CAA40494.1.
PIRiS30539.
RefSeqiNP_001264655.1. NM_001277726.1.
UniGeneiGga.4313.
Gga.46493.
Gga.55806.

3D structure databases

ProteinModelPortaliP34065.
SMRiP34065. Positions 56-256.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP34065.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396003.
KEGGigga:396003.

Organism-specific databases

CTDi5693.

Phylogenomic databases

HOVERGENiHBG108297.
InParanoidiP34065.
KOiK02737.
PhylomeDBiP34065.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Expression of proteasome C1 gene in intercostal muscle of chick embryo."
    Hirosaki Y., Sawada K., Aoyama H.
    Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Primary structure of the smallest subunit of chicken liver proteasome responsible for its chymotrypsin-like activity."
    Shiratsuch A., Sato S.
    Submitted (NOV-1990) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 53-256.
    Tissue: Liver.

Entry informationi

Entry nameiPSB5_CHICK
AccessioniPrimary (citable) accession number: P34065
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: July 15, 1998
Last modified: February 4, 2015
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.