Reviewed, UniProtKB/Swiss-Prot P34064 (PSA5_RAT)

Names and origin

Protein names

Recommended name:
    Proteasome subunit alpha type-5
    EC=3.4.25.1
Alternative name(s):
    Proteasome zeta chain
    Macropain zeta chain
    Multicatalytic endopeptidase complex zeta chain

Gene names

Name:

Psma5

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. PSMA5 interacts directly with the PSMG1-PSMG2 heterodimer which promotes 20S proteasome assembly By similarity.
Subcellular location	Cytoplasm. Nucleus.
Sequence similarities	Belongs to the peptidase T1A family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Threonine protease
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell proteasome core complex Inferred from electronic annotation. Source: InterPro
Molecular function	threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 241	241	Proteasome subunit alpha type-5		PRO_0000124119
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
	Modified residue	16	1	Phosphoserine By similarity
	Modified residue	55	1	Phosphothreonine By similarity
	Modified residue	56	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P34064-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 97491D1974D1483D	FASTA	241	26,391
	10 20 30 40 50 60 MFLTRSEYDR GVNTFSPEGR LFQVEYAIEG HKLGSTAIGI QTSEGVCLAV EKRITSPLME 70 80 90 100 110 120 PSSIEKIVEI DAHIGCAMSG LIADAKTLID KARVETQNHW FTYNETMTVE SVTQAVSNLA 130 140 150 160 170 180 LQFGEEDADP GAMSRPFGVA LLFGGVDEKG PQLFHMDPSG TFVQCDARAI GSASEGAQSS 190 200 210 220 230 240 LQEVYHKSTT LKEAIKSSLI ILKQVMEEKL NATNIELATV QPGQNFHMFT KEELEEVIKD I

References

[1]	"Molecular cloning of cDNAs for rat proteasomes: deduced primary structures of four other subunits." Tamura T., Shimbara N., Aki M., Ishida N., Bey F., Scherrer K., Tanaka K., Ichihara A. J. Biochem. 112:530-534(1992) [PubMed: 1491007] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D10756 mRNA. Translation: BAA01588.1.
IPI	IPI00191502.
PIR	JX0229.
RefSeq	NP_058978.1.
UniGene	Rn.1276
3D structure databases
HSSP	HSSP built from PDB template 1RYP based on UniProtKB P32379.
SMR	P34064. Positions 8-241.
ModBase	Search...
Protein-protein interaction databases
STRING	P34064.
Protein family/group databases
MEROPS	T01.975.
Genome annotation databases
Ensembl	ENSRNOT00000026928; ENSRNOP00000026928; ENSRNOG00000019868; Rattus norvegicus. [Genome view]
GeneID	29672.
KEGG	rno:29672.
Organism-specific databases
CTD	29672.
RGD	61848. Psma5.
Phylogenomic databases
HOVERGEN	P34064.
Enzyme and pathway databases
BRENDA	3.4.25.1. 248.
Gene expression databases
ArrayExpress	P34064.
Genevestigator	P34064.
GermOnline	ENSRNOG00000019868. Rattus norvegicus.
Family and domain databases
InterPro	IPR000426. Proteasome_asu_CS. IPR001353. Proteasome_sua/b. [Graphical view]
Pfam	PF00227. Proteasome. 1 hit. PF10584. Proteasome_A_N. 1 hit. [Graphical view]
PROSITE	PS00388. PROTEASOME_A. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	609999.

Entry information

Entry name

PSA5_RAT

Accession

Primary (citable) accession number: P34064

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	October 13, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)