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Reviewed, UniProtKB/Swiss-Prot P34056 (AP2A_MOUSE)

Last modified June 16, 2009. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Transcription factor AP-2 alpha
      Short name=AP2-alpha
Alternative name(s):
    Activating enhancer-binding protein 2 alpha
    AP-2 transcription factor
    Activator protein 2
      Short name=AP-2
Gene names
Name: Tfap2a
Synonyms: Ap2tf, Tcfap2a
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMus

Protein attributes

Sequence length437 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2 alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle.

Subunit structure

Binds DNA as a dimer. Can form homodimers or heterodimers with other AP-2 family members. Interacts with WWOX. Interacts with UBE2I. Interacts with RALBP1 in a complex also containing EPN1 and NUMB during interphase and mitosis By similarity. Interacts with CITED4.

Subcellular location

Nucleus.

Developmental stage

Expressed from embryo day 9.5 to birth. At day 12.5, expressed in the midbrain, hindbrain, spinal cord, sensory ganglia, epidermis, nephric system and limbs. At mid-embryogenesis, isoform 3 is the most abundant form in the nervous system and total embryo, but the least abundant form in the epidermis. In adults, AP-2A is expressed in the eye, skin, kidney, prostate, thymus, skeletal muscle and very weakly, in the brain. Highest expression found in skin and eye.

Induction

All isoforms are induced during retinoic acid-mediated differentiation and by cAMP stimulation of primary astrocytes. Isoform 3 is most strongly induced in the former case, isoform 1 in the latter case.

Domain

The WW-binding motif mediates interaction with WWOX By similarity.

Post-translational modification

Sumoylated on Lys-10; which inhibits transcriptional activity By similarity.

Sequence similarities

Belongs to the AP-2 family.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P34056-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P34056-2)

The sequence of this isoform differs from the canonical sequence as follows:
     16-160: Missing.
Isoform 3 (identifier: P34056-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: MLWKLTDNIKYEDCE → MLVHSFSAM
Isoform 4 (identifier: P34056-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: MLWKLTDNIKYEDCE → MNSVVVDTPYFGGLPTLGLWNGFSRVVEALRLISSSPSRLAFFQ

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 437437Transcription factor AP-2 alpha
PRO_0000184797

Regions

Region280 – 410131H-S-H (helix-span-helix), dimerization
Motif57 – 626WW-binding
Compositional bias29 – 11789Gln/Pro-rich (transactivation domain)

Amino acid modifications

Modified residue2251Phosphoserine By similarity
Modified residue2391Phosphoserine; by PKA By similarity
Cross-link10Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Natural variations

Alternative sequence1 – 1515MLWKL…YEDCE → MLVHSFSAM in isoform 3.
VSP_006402
Alternative sequence1 – 1515MLWKL…YEDCE → MNSVVVDTPYFGGLPTLGLW NGFSRVVEALRLISSSPSRL AFFQ in isoform 4.
VSP_006403
Alternative sequence16 – 160145Missing in isoform 2.
VSP_006404

Experimental info

Sequence conflict381P → S in AAH07471. Ref.2
Sequence conflict1391A → G Ref.2
Sequence conflict1391A → G Ref.3
Sequence conflict158 – 1592DV → RL Ref.4
Sequence conflict3901Missing Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: 901F456EE07AE69C

FASTA43747,971
        10         20         30         40         50         60 
MLWKLTDNIK YEDCEDRHDG TSNGTARLPQ LGTVGQSPYT SAPPLSHTPN ADFQPPYFPP 

        70         80         90        100        110        120 
PYQPIYPQSQ DPYSHVNDPY SLNPLHAQPQ PQHPGWPGQR QSQESGLLHT HRGLPHQLSG 

       130        140        150        160        170        180 
LDPRRDYRRH EDLLHGPHAL GSGLGDLPIH SLPHAIEDVP HVEDPGINIP DQTVIKKGPV 

       190        200        210        220        230        240 
SLSKSNSNAV SAIPINKDNL FGGVVNPNEV FCSVPGRLSL LSSTSKYKVT VAEVQRRLSP 

       250        260        270        280        290        300 
PECLNASLLG GVLRRAKSKN GGRSLREKLD KIGLNLPAGR RKAANVTLLT SLVEGEAVHL 

       310        320        330        340        350        360 
ARDFGYVCET EFPAKAVAEF LNRQHSDPNE QVARKNMLLA TKQICKEFTD LLAQDRSPLG 

       370        380        390        400        410        420 
NSRPNPILEP GIQSCLTHFN LISHGFGSPA VCAAVTALQN YLTEALKAMD KMYLSNNPNS 

       430 
HTDNSAKSSD KEEKHRK 

« Hide

Isoform 2.

Checksum: 6E9B01E97186E4E1
Show »

FASTA29231,960
Isoform 3.

Checksum: 7CA39A800983538C
Show »

FASTA43147,092
Isoform 4.

Checksum: 49A69CBE5826B8BB
Show »

FASTA46650,900

References

« Hide 'large scale' references
[1]"The complete murine cDNA sequence of the transcription factor AP-2."
Moser M., Pscherer A., Bauer R., Imhof A., Seegers S., Kerscher M., Buettner R.
Nucleic Acids Res. 21:4844-4844(1993) [PubMed: 8233835] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: NIH Swiss.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"Alternative mRNAs encode multiple isoforms of transcription factor AP-2 during murine embryogenesis."
Meier P., Koedood M., Philipp J., Fontana A., Mitchell P.J.
Dev. Biol. 169:1-14(1995) [PubMed: 7750631] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 1-279, ALTERNATIVE SPLICING.
Strain: 129/4 and ICR.
Tissue: Embryo.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 158-437.
Strain: C57BL/6J.
Tissue: Embryonic head.
[5]"Transcription factor AP-2 is expressed in neural crest cell lineages during mouse embryogenesis."
Mitchell P.J., Timmons P.M., Hebert J.M., Rigby P.W.J., Tjian R.
Genes Dev. 5:105-119(1991) [PubMed: 1989904] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 259-437.
[6]"Cloning of mouse Cited4, a member of the CITED family p300/CBP-binding transcriptional coactivators: induced expression in mammary epithelial cells."
Yahata T., Takedatsu H., Dunwoodie S.L., Braganca J., Swingler T., Withington S.L., Hur J., Coser K.R., Isselbacher K.J., Bhattacharya S., Shioda T.
Genomics 80:601-613(2002) [PubMed: 12504852] [Abstract]
Cited for: INTERACTION WITH CITED4.
+Additional computationally mapped references.

Cross-references

Sequence databases

X74216 mRNA. Translation: CAA52292.1.
BC018226 mRNA. Translation: AAH18226.1.
BC007471 mRNA. Translation: AAH07471.1.
U17285 mRNA. Translation: AAA85681.1.
U17291, U17289 Genomic DNA. Translation: AAA85678.1.
U17286 mRNA. Translation: AAA85682.1.
U17287 mRNA. Translation: AAA85683.1.
U17291, U17290 Genomic DNA. Translation: AAA85679.1.
U17288 mRNA. Translation: AAA85684.1.
U17291 Genomic DNA. Translation: AAA85680.1.
AK013900 mRNA. Translation: BAB29047.1.
X57012 mRNA. Translation: CAA40331.1.
IPIIPI00112753.
IPI00226893.
IPI00226894.
IPI00226897.
PIRS42111.
UniGeneMm.85544

3D structure databases

ModBaseSearch...

PTM databases

PhosphoSiteP34056.

Genome annotation databases

EnsemblENSMUSG00000021359. Mus musculus. [Contig view]

Organism-specific databases

MGIMGI:104671. Tcfap2a.

Phylogenomic databases

HOVERGENP34056.

Gene expression databases

ArrayExpressP34056.
BgeeP34056.
CleanExMM_TCFAP2A.
GermOnlineENSMUSG00000021359. Mus musculus.

Family and domain databases

InterProIPR004979. TF_AP2.
IPR008121. TF_AP2_alpha_N.
IPR013854. TF_AP2_C.
[Graphical view]
PANTHERPTHR10812. TF_AP2. 1 hit.
PTHR10812:SF8. TF_AP2_alpha_N. 1 hit.
PfamPF03299. TF_AP-2. 1 hit.
[Graphical view]
PRINTSPR01749. AP2ATNSCPFCT.
PR01748. AP2TNSCPFCT.
ProtoNetSearch...

Other Resources

SOURCESearch...

Entry information

Entry nameAP2A_MOUSE
AccessionPrimary (citable) accession number: P34056
Secondary accession number(s): Q60740 expand/collapse secondary AC list , Q60741, Q60742, Q60743, Q62067, Q62068, Q62069, Q91VX0, Q9CRY4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 1, 1995
Last modified: June 16, 2009
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents