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Protein

Imidazoleglycerol-phosphate dehydratase 1, chloroplastic

Gene

HISN5A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O.

Cofactori

Mn2+Note: Binds 2 manganese ions per subunit.

Pathwayi: L-histidine biosynthesis

This protein is involved in step 6 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. ATP phosphoribosyltransferase 1, chloroplastic (HISN1A), ATP phosphoribosyltransferase 2, chloroplastic (HISN1B)
  2. Histidine biosynthesis bifunctional protein hisIE, chloroplastic (HISN2)
  3. Histidine biosynthesis bifunctional protein hisIE, chloroplastic (HISN2)
  4. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplastic (HISN3)
  5. Imidazole glycerol phosphate synthase hisHF, chloroplastic (HISN4)
  6. Imidazoleglycerol-phosphate dehydratase 2, chloroplastic (HISN5B), Imidazoleglycerol-phosphate dehydratase (HISN5B), Imidazoleglycerol-phosphate dehydratase (AXX17_At3g24180), Imidazoleglycerol-phosphate dehydratase (At3g22425), Imidazoleglycerol-phosphate dehydratase 1, chloroplastic (HISN5A), Imidazoleglycerol-phosphate dehydratase (HISN5B), Imidazoleglycerol-phosphate dehydratase (AXX17_At4g17260)
  7. Histidinol-phosphate aminotransferase 2, chloroplastic (HISN6B), Histidinol-phosphate aminotransferase 1, chloroplastic (HISN6A)
  8. Bifunctional phosphatase IMPL2, chloroplastic (HISN7)
  9. Histidinol dehydrogenase, chloroplastic (HISN8), Histidinol dehydrogenase, chloroplastic (AXX17_At5g63470)
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi110Manganese 11
Metal bindingi136Manganese 21
Metal bindingi137Manganese 11
Metal bindingi140Manganese 21
Metal bindingi208Manganese 21
Metal bindingi232Manganese 11
Metal bindingi233Manganese 21
Metal bindingi236Manganese 11

GO - Molecular functioni

  • imidazoleglycerol-phosphate dehydratase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • histidine biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:AT3G22425-MONOMER.
UniPathwayiUPA00031; UER00011.

Names & Taxonomyi

Protein namesi
Recommended name:
Imidazoleglycerol-phosphate dehydratase 1, chloroplastic (EC:4.2.1.19)
Short name:
IGPD 1
Alternative name(s):
Protein HISTIDINE BIOSYNTHESIS 5A
Gene namesi
Name:HISN5A
Ordered Locus Names:At3g22425
ORF Names:MCB17.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G22425.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 62ChloroplastCombined sourcesAdd BLAST62
ChainiPRO_000015825363 – 270Imidazoleglycerol-phosphate dehydratase 1, chloroplasticAdd BLAST208

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei63N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP34047.

PTM databases

iPTMnetiP34047.

Expressioni

Gene expression databases

GenevisibleiP34047. AT.

Interactioni

Protein-protein interaction databases

BioGridi7144. 1 interactor.
DIPiDIP-48462N.
STRINGi3702.AT3G22425.2.

Structurei

Secondary structure

1270
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi75 – 81Combined sources7
Beta strandi86 – 92Combined sources7
Beta strandi99 – 102Combined sources4
Helixi106 – 119Combined sources14
Beta strandi122 – 128Combined sources7
Turni131 – 133Combined sources3
Helixi136 – 154Combined sources19
Beta strandi166 – 170Combined sources5
Beta strandi173 – 180Combined sources8
Beta strandi186 – 190Combined sources5
Beta strandi194 – 198Combined sources5
Helixi205 – 217Combined sources13
Beta strandi220 – 227Combined sources8
Helixi231 – 250Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2F1DX-ray3.00A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P64-270[»]
ProteinModelPortaliP34047.
SMRiP34047.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP34047.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3143. Eukaryota.
COG0131. LUCA.
HOGENOMiHOG000228064.
KOiK01693.
OMAiHHIAESC.
OrthoDBiEOG09360JJC.
PhylomeDBiP34047.

Family and domain databases

CDDicd07914. IGPD. 1 hit.
HAMAPiMF_00076. HisB. 1 hit.
InterProiIPR000807. ImidazoleglycerolP_deHydtase.
IPR020565. ImidazoleglycerP_deHydtase_CS.
IPR020568. Ribosomal_S5_D2-typ_fold.
[Graphical view]
PfamiPF00475. IGPD. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 2 hits.
PROSITEiPS00954. IGP_DEHYDRATASE_1. 1 hit.
PS00955. IGP_DEHYDRATASE_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Experimental confirmation may be lacking for some isoforms.
Isoform 1 (identifier: P34047-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MELSSASAIL SHSSSAAQLL RPKLGFIDLL PRRAMIVSSP SSSLPRFLRM
60 70 80 90 100
ESQSQLRQSI SCSASSSSSM ALGRIGEVKR VTKETNVSVK INLDGTGVAD
110 120 130 140 150
SSSGIPFLDH MLDQLASHGL FDVHVRATGD VHIDDHHTNE DIALAIGTAL
160 170 180 190 200
LKALGERKGI NRFGDFTAPL DEALIHVSLD LSGRPYLGYN LEIPTQRVGT
210 220 230 240 250
YDTQLVEHFF QSLVNTSGMT LHIRQLAGEN SHHIIEATFK AFARALRQAT
260 270
ETDPRRGGTI PSSKGVLSRS
Length:270
Mass (Da):29,225
Last modified:February 1, 1994 - v1
Checksum:i7132D80CC687E20C
GO
Isoform 2 (identifier: P34047-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     205-270: LVEHFFQSLV...PSSKGVLSRS → VLSLLLELSS...YDSSHPAARW

Note: May be due to an intron retention.
Show »
Length:264
Mass (Da):28,691
Checksum:i8551F5DED1EB8CAD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti201Y → C in BAD44082 (Ref. 6) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008895205 – 270LVEHF…VLSRS → VLSLLLELSSFGFICVIRCL VIIESVAKNCLTFRFVVGGA LFPVVGEYFWYDSSHPAARW in isoform 2. 1 PublicationAdd BLAST66

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02689 mRNA. Translation: AAA93196.1.
AB022215 Genomic DNA. Translation: BAB01781.1.
CP002686 Genomic DNA. Translation: AEE76636.1.
CP002686 Genomic DNA. Translation: AEE76637.1.
AK118815 mRNA. Translation: BAC43405.1.
AY070442 mRNA. Translation: AAL49845.1.
AK176319 mRNA. Translation: BAD44082.1.
AK176429 mRNA. Translation: BAD44192.1.
AY087948 mRNA. Translation: AAM65496.1.
RefSeqiNP_850624.1. NM_180293.1. [P34047-2]
NP_850625.1. NM_180294.2. [P34047-1]
UniGeneiAt.19962.

Genome annotation databases

EnsemblPlantsiAT3G22425.2; AT3G22425.2; AT3G22425. [P34047-1]
GeneIDi821812.
GrameneiAT3G22425.2; AT3G22425.2; AT3G22425.
KEGGiath:AT3G22425.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02689 mRNA. Translation: AAA93196.1.
AB022215 Genomic DNA. Translation: BAB01781.1.
CP002686 Genomic DNA. Translation: AEE76636.1.
CP002686 Genomic DNA. Translation: AEE76637.1.
AK118815 mRNA. Translation: BAC43405.1.
AY070442 mRNA. Translation: AAL49845.1.
AK176319 mRNA. Translation: BAD44082.1.
AK176429 mRNA. Translation: BAD44192.1.
AY087948 mRNA. Translation: AAM65496.1.
RefSeqiNP_850624.1. NM_180293.1. [P34047-2]
NP_850625.1. NM_180294.2. [P34047-1]
UniGeneiAt.19962.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2F1DX-ray3.00A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P64-270[»]
ProteinModelPortaliP34047.
SMRiP34047.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi7144. 1 interactor.
DIPiDIP-48462N.
STRINGi3702.AT3G22425.2.

PTM databases

iPTMnetiP34047.

Proteomic databases

PaxDbiP34047.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G22425.2; AT3G22425.2; AT3G22425. [P34047-1]
GeneIDi821812.
GrameneiAT3G22425.2; AT3G22425.2; AT3G22425.
KEGGiath:AT3G22425.

Organism-specific databases

TAIRiAT3G22425.

Phylogenomic databases

eggNOGiKOG3143. Eukaryota.
COG0131. LUCA.
HOGENOMiHOG000228064.
KOiK01693.
OMAiHHIAESC.
OrthoDBiEOG09360JJC.
PhylomeDBiP34047.

Enzyme and pathway databases

UniPathwayiUPA00031; UER00011.
BioCyciMetaCyc:AT3G22425-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP34047.
PROiP34047.

Gene expression databases

GenevisibleiP34047. AT.

Family and domain databases

CDDicd07914. IGPD. 1 hit.
HAMAPiMF_00076. HisB. 1 hit.
InterProiIPR000807. ImidazoleglycerolP_deHydtase.
IPR020565. ImidazoleglycerP_deHydtase_CS.
IPR020568. Ribosomal_S5_D2-typ_fold.
[Graphical view]
PfamiPF00475. IGPD. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 2 hits.
PROSITEiPS00954. IGP_DEHYDRATASE_1. 1 hit.
PS00955. IGP_DEHYDRATASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHIS5A_ARATH
AccessioniPrimary (citable) accession number: P34047
Secondary accession number(s): Q67YN9, Q67YZ9, Q8VYM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 30, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.