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P34038 (KPYK_LACDE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate kinase

Short name=PK
EC=2.7.1.40
Gene names
Name:pyk
OrganismLactobacillus delbrueckii subsp. bulgaricus
Taxonomic identifier1585 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length589 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium.

Potassium.

Enzyme regulation

Strongly activated by glucose-6-phosphate, ribose-5-phosphate and fructose-6-phosphate. Weak activator AMP and weak inhibitor fructose-1,6-bisphosphate can act as strong inhibitors in the presence of strong activators.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer.

Sequence similarities

Belongs to the pyruvate kinase family.

In the C-terminal section; belongs to the PEP-utilizing enzyme family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 589589Pyruvate kinase
PRO_0000112076

Sites

Metal binding341Potassium By similarity
Metal binding361Potassium By similarity
Metal binding661Potassium By similarity
Metal binding671Potassium; via carbonyl oxygen By similarity
Metal binding2231Magnesium By similarity
Metal binding2471Magnesium By similarity
Binding site321Substrate By similarity
Binding site2461Substrate; via amide nitrogen By similarity
Binding site2471Substrate; via amide nitrogen By similarity
Binding site2791Substrate By similarity
Site2211Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
P34038 [UniParc].

Last modified February 1, 1996. Version 3.
Checksum: 2BA3D3CF7AE290F1

FASTA58962,920
        10         20         30         40         50         60 
MKKTKIVSTL GPASDDIETI TKLAEAGANV FRFNFSHGNH EEHLARMNMV REVEKKTGKL 

        70         80         90        100        110        120 
LGIALDTKGA EIRTTDQEGG KFTINTGDEI RVSMDATKAG NKDMIHVTYP GLFDDTHVGG 

       130        140        150        160        170        180 
TVLIDDGAVG LTIKAKDEEK RELVCEAQNT GVIGSKKGVN APGVEIRLPG ITEKDTDDIR 

       190        200        210        220        230        240 
FGLKHGINFI FASFVRKAQD VLDIRALCEE ANASYVKIFP KIESQEGIDN IDEILQVSDG 

       250        260        270        280        290        300 
LMVARGDMGV EIPFINVPFV QKTLIKKCNA LGKPVITATQ MLDSMQENPR PTRAEVTDVA 

       310        320        330        340        350        360 
NAVLDGTDAT MLSGESANGL YPVQSVQAMH DIDVRTEKEL DTRNTLALQR FEEYKGSNVT 

       370        380        390        400        410        420 
EAIGESVVRT AQELGVKTII AATSSGYTAR MISKYRPDAT IVALTFDEKI QHSLGIVWGV 

       430        440        450        460        470        480 
EPVLAKKPSN TDEMFEEAAR VAKEHGFVKD GDLVIIVAGV PFGQSGTTNL MKLQIIGNQL 

       490        500        510        520        530        540 
AQGLGVGTGS VIGKAVVANS AEEANAKVHE GDILVAKTTD KDYMPAIKKA SGMIVEASGL 

       550        560        570        580 
TSHAAVVGVS LGIPVVVGVA DATSKIADGS TLTVDARRGA IYQGEVSNL 

« Hide

References

[1]"The genes for phosphofructokinase and pyruvate kinase of Lactobacillus delbrueckii subsp. bulgaricus constitute an operon."
Branny P., de la Torre F., Garel J.R.
J. Bacteriol. 178:4727-4730(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Cloning, sequencing, and expression in Escherichia coli of the gene coding for phosphofructokinase in Lactobacillus bulgaricus."
Branny P., de la Torre F., Garel J.R.
J. Bacteriol. 175:5344-5349(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-60.
[3]"Pyruvate kinase from Lactobacillus bulgaricus: possible regulation by competition between strong and weak effectors."
le Bras G., Garel J.R.
Biochimie 75:797-802(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-40, CHARACTERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X71403 Genomic DNA. Translation: CAA50527.1.
PIRB48663.
S35929.

3D structure databases

ProteinModelPortalP34038.
SMRP34038. Positions 1-589.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00109; UER00188.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
3.50.30.10. 1 hit.
InterProIPR008279. PEP-util_enz_mobile_dom.
IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00391. PEP-utilizers. 1 hit.
PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
ProtoNetSearch...

Entry information

Entry nameKPYK_LACDE
AccessionPrimary (citable) accession number: P34038
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 93 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways