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P34038

- KPYK_LACDE

UniProt

P34038 - KPYK_LACDE

Protein

Pyruvate kinase

Gene

pyk

Organism
Lactobacillus delbrueckii subsp. bulgaricus
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 3 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.
    Potassium.

    Enzyme regulationi

    Strongly activated by glucose-6-phosphate, ribose-5-phosphate and fructose-6-phosphate. Weak activator AMP and weak inhibitor fructose-1,6-bisphosphate can act as strong inhibitors in the presence of strong activators.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei32 – 321SubstrateBy similarity
    Metal bindingi34 – 341PotassiumBy similarity
    Metal bindingi36 – 361PotassiumBy similarity
    Metal bindingi66 – 661PotassiumBy similarity
    Metal bindingi67 – 671Potassium; via carbonyl oxygenBy similarity
    Sitei221 – 2211Transition state stabilizerBy similarity
    Metal bindingi223 – 2231MagnesiumBy similarity
    Binding sitei246 – 2461Substrate; via amide nitrogenBy similarity
    Metal bindingi247 – 2471MagnesiumBy similarity
    Binding sitei247 – 2471Substrate; via amide nitrogenBy similarity
    Binding sitei279 – 2791SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase (EC:2.7.1.40)
    Short name:
    PK
    Gene namesi
    Name:pyk
    OrganismiLactobacillus delbrueckii subsp. bulgaricus
    Taxonomic identifieri1585 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 589589Pyruvate kinasePRO_0000112076Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.

    Structurei

    3D structure databases

    ProteinModelPortaliP34038.
    SMRiP34038. Positions 1-589.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated
    In the C-terminal section; belongs to the PEP-utilizing enzyme family.Curated

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    3.50.30.10. 1 hit.
    InterProiIPR008279. PEP-util_enz_mobile_dom.
    IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00391. PEP-utilizers. 1 hit.
    PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P34038-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKTKIVSTL GPASDDIETI TKLAEAGANV FRFNFSHGNH EEHLARMNMV    50
    REVEKKTGKL LGIALDTKGA EIRTTDQEGG KFTINTGDEI RVSMDATKAG 100
    NKDMIHVTYP GLFDDTHVGG TVLIDDGAVG LTIKAKDEEK RELVCEAQNT 150
    GVIGSKKGVN APGVEIRLPG ITEKDTDDIR FGLKHGINFI FASFVRKAQD 200
    VLDIRALCEE ANASYVKIFP KIESQEGIDN IDEILQVSDG LMVARGDMGV 250
    EIPFINVPFV QKTLIKKCNA LGKPVITATQ MLDSMQENPR PTRAEVTDVA 300
    NAVLDGTDAT MLSGESANGL YPVQSVQAMH DIDVRTEKEL DTRNTLALQR 350
    FEEYKGSNVT EAIGESVVRT AQELGVKTII AATSSGYTAR MISKYRPDAT 400
    IVALTFDEKI QHSLGIVWGV EPVLAKKPSN TDEMFEEAAR VAKEHGFVKD 450
    GDLVIIVAGV PFGQSGTTNL MKLQIIGNQL AQGLGVGTGS VIGKAVVANS 500
    AEEANAKVHE GDILVAKTTD KDYMPAIKKA SGMIVEASGL TSHAAVVGVS 550
    LGIPVVVGVA DATSKIADGS TLTVDARRGA IYQGEVSNL 589
    Length:589
    Mass (Da):62,920
    Last modified:February 1, 1996 - v3
    Checksum:i2BA3D3CF7AE290F1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X71403 Genomic DNA. Translation: CAA50527.1.
    PIRiB48663.
    S35929.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X71403 Genomic DNA. Translation: CAA50527.1 .
    PIRi B48663.
    S35929.

    3D structure databases

    ProteinModelPortali P34038.
    SMRi P34038. Positions 1-589.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    3.50.30.10. 1 hit.
    InterProi IPR008279. PEP-util_enz_mobile_dom.
    IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00391. PEP-utilizers. 1 hit.
    PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genes for phosphofructokinase and pyruvate kinase of Lactobacillus delbrueckii subsp. bulgaricus constitute an operon."
      Branny P., de la Torre F., Garel J.R.
      J. Bacteriol. 178:4727-4730(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Cloning, sequencing, and expression in Escherichia coli of the gene coding for phosphofructokinase in Lactobacillus bulgaricus."
      Branny P., de la Torre F., Garel J.R.
      J. Bacteriol. 175:5344-5349(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-60.
    3. "Pyruvate kinase from Lactobacillus bulgaricus: possible regulation by competition between strong and weak effectors."
      le Bras G., Garel J.R.
      Biochimie 75:797-802(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-40, CHARACTERIZATION.

    Entry informationi

    Entry nameiKPYK_LACDE
    AccessioniPrimary (citable) accession number: P34038
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 95 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3