P34030 (GYRA_SPICI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Gene names

Name:

gyrA

Organism

Spiroplasma citri

Taxonomic identifier

2133 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Entomoplasmatales › Spiroplasmataceae › Spiroplasma

Protein attributes

Sequence length	227 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP-Rule MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_01897
Subunit structure	Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Gene Ontology (GO)
Biological_process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular_component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type II (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›227	›227	DNA gyrase subunit A HAMAP-Rule MF_01897		PRO_0000145250
Sites
	Active site	129	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Experimental info
	Non-terminal residue	227	1

Sequences

	Sequence		Length	Mass (Da)
	P34030 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 476C66482A9010A9	FASTA	227	25,356
	10 20 30 40 50 60 MMKSENDGYD YDGKIRDIDI ADEMKNGFLD YAMSVIVSRA IPDVRDGLKP VHRRIIYAMW 70 80 90 100 110 120 DLKMTYEKQH KKSARIVGEV IGKYHPHGDT AVYEAMVRMA QDFSYRYPLI DGHGNFGSMD 130 140 150 160 170 180 GDPPAAMRYT EAKMSKIAGE IIKDIEKETT IFIDNYDGSE EEPTFLPGYF PNLLVNGASG 190 200 210 220 IAVGMATNIP PHNLNEVIDG VIAVTKNPEI TTVELMKIIK GPDFPTG

References

[1]

"Cloning and sequencing of the replication origin (oriC) of the Spiroplasma citri chromosome and construction of autonomously replicating artificial plasmids." Ye F., Renaudin J., Bove J.M., Laigret F. Curr. Microbiol. 29:23-29(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: R8A2HP.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z19108 Genomic DNA. Translation: CAA79524.1.
PIR	S35735.
3D structure databases
ProteinModelPortal	P34030.
SMR	P34030. Positions 37-227.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.90.199.10. 1 hit.
HAMAP	MF_01897. GyrA.
InterPro	IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013760. Topo_IIA_like_dom. [Graphical view]
Pfam	PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_SPICI

Accession

Primary (citable) accession number: P34030

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program