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Protein

1-phosphatidylinositol phosphodiesterase

Gene

plcA

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves glycosylphosphatidylinositol (GPI) and phosphatidylinositol (PI) anchors but not PI phosphates. Important factor in pathogenesis, PI-PLC activity is present only in virulent listeria species. It may participate in the lysis of the phagolysosomal membrane.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol = 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei67 – 671Proton acceptor
Active sitei115 – 1151Proton donor

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Lipid degradation, Lipid metabolism, Virulence

Enzyme and pathway databases

BioCyciLMON169963:LMO0201-MONOMER.
BRENDAi3.1.4.11. 3045.
4.6.1.13. 3045.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol phosphodiesterase (EC:4.6.1.13)
Alternative name(s):
Phosphatidylinositol diacylglycerol-lyase
Phosphatidylinositol-specific phospholipase C
Short name:
PI-PLC
Gene namesi
Name:plcA
Synonyms:pic
Ordered Locus Names:lmo0201
OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Taxonomic identifieri169963 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
ProteomesiUP000000817 Componenti: Chromosome

Organism-specific databases

GenoListiLMO0201.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 3172951-phosphatidylinositol phosphodiesterasePRO_0000024296Add
BLAST

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi169963.lmo0201.

Structurei

Secondary structure

1
317
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi45 – 473Combined sources
Helixi49 – 513Combined sources
Helixi58 – 603Combined sources
Beta strandi61 – 666Combined sources
Turni67 – 726Combined sources
Helixi76 – 805Combined sources
Helixi82 – 854Combined sources
Helixi92 – 976Combined sources
Beta strandi102 – 1076Combined sources
Beta strandi111 – 1155Combined sources
Beta strandi118 – 1236Combined sources
Helixi124 – 13714Combined sources
Beta strandi143 – 1497Combined sources
Helixi156 – 16712Combined sources
Helixi168 – 1714Combined sources
Beta strandi174 – 1785Combined sources
Turni187 – 1926Combined sources
Beta strandi194 – 2007Combined sources
Beta strandi206 – 2083Combined sources
Beta strandi211 – 2166Combined sources
Beta strandi222 – 2254Combined sources
Helixi233 – 24917Combined sources
Beta strandi251 – 2588Combined sources
Beta strandi264 – 2663Combined sources
Helixi268 – 28821Combined sources
Beta strandi295 – 3017Combined sources
Helixi304 – 3118Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AODX-ray2.60A29-317[»]
2PLCX-ray2.00A43-316[»]
ProteinModelPortaliP34024.
SMRiP34024. Positions 43-316.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP34024.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini58 – 196139PI-PLC X-boxPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG14688.
KOiK01771.
OMAiMPGTHNT.
OrthoDBiEOG6091B5.

Family and domain databases

Gene3Di3.20.20.190. 1 hit.
InterProiIPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
[Graphical view]
PfamiPF00388. PI-PLC-X. 1 hit.
[Graphical view]
SMARTiSM00148. PLCXc. 1 hit.
[Graphical view]
SUPFAMiSSF51695. SSF51695. 1 hit.
PROSITEiPS50007. PIPLC_X_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P34024-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYKNYLQRTL VLLLCFILYF FTFPLGGKAY SLNNWNKPIK NSVTTKQWMS
60 70 80 90 100
ALPDTTNLAA LSIPGTHDTM SYNGDITWTL TKPLAQTQTM SLYQQLEAGI
110 120 130 140 150
RYIDIRAKDN LNIYHGPIFL NASLSGVLET ITQFLKKNPK ETIIMRLKDE
160 170 180 190 200
QNSNDSFDYR IQPLINIYKD YFYTTPRTDT SNKIPTLKDV RGKILLLSEN
210 220 230 240 250
HTKKPLVINS RKFGMQFGAP NQVIQDDYNG PSVKTKFKEI VQTAYQASKA
260 270 280 290 300
DNKLFLNHIS ATSLTFTPRQ YAAALNNKVE QFVLNLTSEK VRGLGILIMD
310
FPEKQTIKNI IKNNKFN
Length:317
Mass (Da):36,318
Last modified:February 1, 1994 - v1
Checksum:i5D0E33649A983DA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54618 Genomic DNA. Translation: CAA38438.1.
AL591974 Genomic DNA. Translation: CAD00728.1.
PIRiAB1100.
S15336. A37204.
RefSeqiNP_463732.1. NC_003210.1.
WP_003733144.1. NC_003210.1.

Genome annotation databases

EnsemblBacteriaiCAD00728; CAD00728; CAD00728.
GeneIDi987032.
KEGGilmo:lmo0201.
PATRICi20309409. VBILisMon69206_0206.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54618 Genomic DNA. Translation: CAA38438.1.
AL591974 Genomic DNA. Translation: CAD00728.1.
PIRiAB1100.
S15336. A37204.
RefSeqiNP_463732.1. NC_003210.1.
WP_003733144.1. NC_003210.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AODX-ray2.60A29-317[»]
2PLCX-ray2.00A43-316[»]
ProteinModelPortaliP34024.
SMRiP34024. Positions 43-316.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi169963.lmo0201.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAD00728; CAD00728; CAD00728.
GeneIDi987032.
KEGGilmo:lmo0201.
PATRICi20309409. VBILisMon69206_0206.

Organism-specific databases

GenoListiLMO0201.

Phylogenomic databases

eggNOGiNOG14688.
KOiK01771.
OMAiMPGTHNT.
OrthoDBiEOG6091B5.

Enzyme and pathway databases

BioCyciLMON169963:LMO0201-MONOMER.
BRENDAi3.1.4.11. 3045.
4.6.1.13. 3045.

Miscellaneous databases

EvolutionaryTraceiP34024.

Family and domain databases

Gene3Di3.20.20.190. 1 hit.
InterProiIPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
[Graphical view]
PfamiPF00388. PI-PLC-X. 1 hit.
[Graphical view]
SMARTiSM00148. PLCXc. 1 hit.
[Graphical view]
SUPFAMiSSF51695. SSF51695. 1 hit.
PROSITEiPS50007. PIPLC_X_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of phosphatidylinositol-specific phospholipase C activity in Listeria monocytogenes: a novel type of virulence factor?"
    Mengaud J., Braun-Breton C., Cossart P.
    Mol. Microbiol. 5:367-372(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LO28 / Serovar 1/2c.
  2. "Detection of a gene encoding a phosphatidylinositol-specific phospholipase C that is co-ordinately expressed with listeriolysin in Listeria monocytogenes."
    Leimeister-Waechter M., Domann E., Chakraborty T.
    Mol. Microbiol. 5:361-366(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: EGD / Serovar 1/2a.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-679 / EGD-e.
  4. "Transcriptional mapping and nucleotide sequence of the Listeria monocytogenes hlyA region reveal structural features that may be involved in regulation."
    Mengaud J., Vicente M.-F., Cossart P.
    Infect. Immun. 57:3695-3701(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 70-317.
  5. "Crystal structure of the phosphatidylinositol-specific phospholipase C from the human pathogen Listeria monocytogenes."
    Moser J., Gerstel B., Meyer J.E., Chakraborty T., Wehland J., Heinz D.W.
    J. Mol. Biol. 273:269-282(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 43-317.
    Strain: EGD / Serovar 1/2a.

Entry informationi

Entry nameiPLC_LISMO
AccessioniPrimary (citable) accession number: P34024
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 27, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.