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Protein

1-phosphatidylinositol phosphodiesterase

Gene

plcA

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves glycosylphosphatidylinositol (GPI) and phosphatidylinositol (PI) anchors but not PI phosphates. Important factor in pathogenesis, PI-PLC activity is present only in virulent listeria species. It may participate in the lysis of the phagolysosomal membrane.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol = 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei67Proton acceptor1
Active sitei115Proton donor1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Lipid degradation, Lipid metabolism, Virulence

Enzyme and pathway databases

BRENDAi3.1.4.11. 3045.
4.6.1.13. 3045.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol phosphodiesterase (EC:4.6.1.13)
Alternative name(s):
Phosphatidylinositol diacylglycerol-lyase
Phosphatidylinositol-specific phospholipase C
Short name:
PI-PLC
Gene namesi
Name:plcA
Synonyms:pic
Ordered Locus Names:lmo0201
OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Taxonomic identifieri169963 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
Proteomesi
  • UP000000817 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000002429623 – 3171-phosphatidylinositol phosphodiesteraseAdd BLAST295

Proteomic databases

PaxDbiP34024.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi169963.lmo0201.

Structurei

Secondary structure

1317
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi45 – 47Combined sources3
Helixi49 – 51Combined sources3
Helixi58 – 60Combined sources3
Beta strandi61 – 66Combined sources6
Turni67 – 72Combined sources6
Helixi76 – 80Combined sources5
Helixi82 – 85Combined sources4
Helixi92 – 97Combined sources6
Beta strandi102 – 107Combined sources6
Beta strandi111 – 115Combined sources5
Beta strandi118 – 123Combined sources6
Helixi124 – 137Combined sources14
Beta strandi143 – 149Combined sources7
Helixi156 – 167Combined sources12
Helixi168 – 171Combined sources4
Beta strandi174 – 178Combined sources5
Turni187 – 192Combined sources6
Beta strandi194 – 200Combined sources7
Beta strandi206 – 208Combined sources3
Beta strandi211 – 216Combined sources6
Beta strandi222 – 225Combined sources4
Helixi233 – 249Combined sources17
Beta strandi251 – 258Combined sources8
Beta strandi264 – 266Combined sources3
Helixi268 – 288Combined sources21
Beta strandi295 – 301Combined sources7
Helixi304 – 311Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AODX-ray2.60A29-317[»]
2PLCX-ray2.00A43-316[»]
ProteinModelPortaliP34024.
SMRiP34024.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP34024.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini58 – 196PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST139

Sequence similaritiesi

Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108NQ3. Bacteria.
ENOG410ZKFY. LUCA.
KOiK01771.
OMAiMPGTHNT.

Family and domain databases

Gene3Di3.20.20.190. 1 hit.
InterProiIPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
[Graphical view]
PfamiPF00388. PI-PLC-X. 1 hit.
[Graphical view]
SMARTiSM00148. PLCXc. 1 hit.
[Graphical view]
SUPFAMiSSF51695. SSF51695. 1 hit.
PROSITEiPS50007. PIPLC_X_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P34024-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYKNYLQRTL VLLLCFILYF FTFPLGGKAY SLNNWNKPIK NSVTTKQWMS
60 70 80 90 100
ALPDTTNLAA LSIPGTHDTM SYNGDITWTL TKPLAQTQTM SLYQQLEAGI
110 120 130 140 150
RYIDIRAKDN LNIYHGPIFL NASLSGVLET ITQFLKKNPK ETIIMRLKDE
160 170 180 190 200
QNSNDSFDYR IQPLINIYKD YFYTTPRTDT SNKIPTLKDV RGKILLLSEN
210 220 230 240 250
HTKKPLVINS RKFGMQFGAP NQVIQDDYNG PSVKTKFKEI VQTAYQASKA
260 270 280 290 300
DNKLFLNHIS ATSLTFTPRQ YAAALNNKVE QFVLNLTSEK VRGLGILIMD
310
FPEKQTIKNI IKNNKFN
Length:317
Mass (Da):36,318
Last modified:February 1, 1994 - v1
Checksum:i5D0E33649A983DA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54618 Genomic DNA. Translation: CAA38438.1.
AL591974 Genomic DNA. Translation: CAD00728.1.
PIRiAB1100.
S15336. A37204.
RefSeqiNP_463732.1. NC_003210.1.
WP_003733144.1. NC_003210.1.

Genome annotation databases

EnsemblBacteriaiCAD00728; CAD00728; CAD00728.
GeneIDi987032.
KEGGilmo:lmo0201.
PATRICi20309409. VBILisMon69206_0206.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54618 Genomic DNA. Translation: CAA38438.1.
AL591974 Genomic DNA. Translation: CAD00728.1.
PIRiAB1100.
S15336. A37204.
RefSeqiNP_463732.1. NC_003210.1.
WP_003733144.1. NC_003210.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AODX-ray2.60A29-317[»]
2PLCX-ray2.00A43-316[»]
ProteinModelPortaliP34024.
SMRiP34024.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi169963.lmo0201.

Proteomic databases

PaxDbiP34024.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAD00728; CAD00728; CAD00728.
GeneIDi987032.
KEGGilmo:lmo0201.
PATRICi20309409. VBILisMon69206_0206.

Phylogenomic databases

eggNOGiENOG4108NQ3. Bacteria.
ENOG410ZKFY. LUCA.
KOiK01771.
OMAiMPGTHNT.

Enzyme and pathway databases

BRENDAi3.1.4.11. 3045.
4.6.1.13. 3045.

Miscellaneous databases

EvolutionaryTraceiP34024.

Family and domain databases

Gene3Di3.20.20.190. 1 hit.
InterProiIPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
[Graphical view]
PfamiPF00388. PI-PLC-X. 1 hit.
[Graphical view]
SMARTiSM00148. PLCXc. 1 hit.
[Graphical view]
SUPFAMiSSF51695. SSF51695. 1 hit.
PROSITEiPS50007. PIPLC_X_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLC_LISMO
AccessioniPrimary (citable) accession number: P34024
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.