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P34024

- PLC_LISMO

UniProt

P34024 - PLC_LISMO

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Protein

1-phosphatidylinositol phosphodiesterase

Gene

plcA

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Cleaves glycosylphosphatidylinositol (GPI) and phosphatidylinositol (PI) anchors but not PI phosphates. Important factor in pathogenesis, PI-PLC activity is present only in virulent listeria species. It may participate in the lysis of the phagolysosomal membrane.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol = 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei67 – 671Proton acceptor
Active sitei115 – 1151Proton donor

GO - Molecular functioni

  1. phosphatidylinositol diacylglycerol-lyase activity Source: UniProtKB-EC
  2. phosphoric diester hydrolase activity Source: InterPro

GO - Biological processi

  1. lipid catabolic process Source: UniProtKB-KW
  2. pathogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Lipid degradation, Lipid metabolism, Virulence

Enzyme and pathway databases

BioCyciLMON169963:LMO0201-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol phosphodiesterase (EC:4.6.1.13)
Alternative name(s):
Phosphatidylinositol diacylglycerol-lyase
Phosphatidylinositol-specific phospholipase C
Short name:
PI-PLC
Gene namesi
Name:plcA
Synonyms:pic
Ordered Locus Names:lmo0201
OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Taxonomic identifieri169963 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
ProteomesiUP000000817: Chromosome

Organism-specific databases

GenoListiLMO0201.

Subcellular locationi

Secreted. Cytoplasm
Note: Secreted and, to a lesser extent, cytoplasmic.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 3172951-phosphatidylinositol phosphodiesterasePRO_0000024296Add
BLAST

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi169963.lmo0201.

Structurei

Secondary structure

1
317
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi45 – 473
Helixi49 – 513
Helixi58 – 603
Beta strandi61 – 666
Turni67 – 726
Helixi76 – 805
Helixi82 – 854
Helixi92 – 976
Beta strandi102 – 1076
Beta strandi111 – 1155
Beta strandi118 – 1236
Helixi124 – 13714
Beta strandi143 – 1497
Helixi156 – 16712
Helixi168 – 1714
Beta strandi174 – 1785
Turni187 – 1926
Beta strandi194 – 2007
Beta strandi206 – 2083
Beta strandi211 – 2166
Beta strandi222 – 2254
Helixi233 – 24917
Beta strandi251 – 2588
Beta strandi264 – 2663
Helixi268 – 28821
Beta strandi295 – 3017
Helixi304 – 3118

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AODX-ray2.60A29-317[»]
2PLCX-ray2.00A43-316[»]
ProteinModelPortaliP34024.
SMRiP34024. Positions 43-316.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP34024.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini58 – 196139PI-PLC X-boxPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG14688.
KOiK01771.
OMAiDLWIIPD.
OrthoDBiEOG6091B5.

Family and domain databases

Gene3Di3.20.20.190. 1 hit.
InterProiIPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
[Graphical view]
PfamiPF00388. PI-PLC-X. 1 hit.
[Graphical view]
SMARTiSM00148. PLCXc. 1 hit.
[Graphical view]
SUPFAMiSSF51695. SSF51695. 1 hit.
PROSITEiPS50007. PIPLC_X_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P34024 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYKNYLQRTL VLLLCFILYF FTFPLGGKAY SLNNWNKPIK NSVTTKQWMS
60 70 80 90 100
ALPDTTNLAA LSIPGTHDTM SYNGDITWTL TKPLAQTQTM SLYQQLEAGI
110 120 130 140 150
RYIDIRAKDN LNIYHGPIFL NASLSGVLET ITQFLKKNPK ETIIMRLKDE
160 170 180 190 200
QNSNDSFDYR IQPLINIYKD YFYTTPRTDT SNKIPTLKDV RGKILLLSEN
210 220 230 240 250
HTKKPLVINS RKFGMQFGAP NQVIQDDYNG PSVKTKFKEI VQTAYQASKA
260 270 280 290 300
DNKLFLNHIS ATSLTFTPRQ YAAALNNKVE QFVLNLTSEK VRGLGILIMD
310
FPEKQTIKNI IKNNKFN
Length:317
Mass (Da):36,318
Last modified:February 1, 1994 - v1
Checksum:i5D0E33649A983DA2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X54618 Genomic DNA. Translation: CAA38438.1.
AL591974 Genomic DNA. Translation: CAD00728.1.
PIRiAB1100.
S15336. A37204.
RefSeqiNP_463732.1. NC_003210.1.
WP_003733144.1. NC_003210.1.

Genome annotation databases

EnsemblBacteriaiCAD00728; CAD00728; CAD00728.
GeneIDi987032.
KEGGilmo:lmo0201.
PATRICi20309409. VBILisMon69206_0206.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X54618 Genomic DNA. Translation: CAA38438.1 .
AL591974 Genomic DNA. Translation: CAD00728.1 .
PIRi AB1100.
S15336. A37204.
RefSeqi NP_463732.1. NC_003210.1.
WP_003733144.1. NC_003210.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1AOD X-ray 2.60 A 29-317 [» ]
2PLC X-ray 2.00 A 43-316 [» ]
ProteinModelPortali P34024.
SMRi P34024. Positions 43-316.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 169963.lmo0201.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD00728 ; CAD00728 ; CAD00728 .
GeneIDi 987032.
KEGGi lmo:lmo0201.
PATRICi 20309409. VBILisMon69206_0206.

Organism-specific databases

GenoListi LMO0201.

Phylogenomic databases

eggNOGi NOG14688.
KOi K01771.
OMAi DLWIIPD.
OrthoDBi EOG6091B5.

Enzyme and pathway databases

BioCyci LMON169963:LMO0201-MONOMER.

Miscellaneous databases

EvolutionaryTracei P34024.

Family and domain databases

Gene3Di 3.20.20.190. 1 hit.
InterProi IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
[Graphical view ]
Pfami PF00388. PI-PLC-X. 1 hit.
[Graphical view ]
SMARTi SM00148. PLCXc. 1 hit.
[Graphical view ]
SUPFAMi SSF51695. SSF51695. 1 hit.
PROSITEi PS50007. PIPLC_X_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of phosphatidylinositol-specific phospholipase C activity in Listeria monocytogenes: a novel type of virulence factor?"
    Mengaud J., Braun-Breton C., Cossart P.
    Mol. Microbiol. 5:367-372(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LO28 / Serovar 1/2c.
  2. "Detection of a gene encoding a phosphatidylinositol-specific phospholipase C that is co-ordinately expressed with listeriolysin in Listeria monocytogenes."
    Leimeister-Waechter M., Domann E., Chakraborty T.
    Mol. Microbiol. 5:361-366(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: EGD / Serovar 1/2a.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-679 / EGD-e.
  4. "Transcriptional mapping and nucleotide sequence of the Listeria monocytogenes hlyA region reveal structural features that may be involved in regulation."
    Mengaud J., Vicente M.-F., Cossart P.
    Infect. Immun. 57:3695-3701(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 70-317.
  5. "Crystal structure of the phosphatidylinositol-specific phospholipase C from the human pathogen Listeria monocytogenes."
    Moser J., Gerstel B., Meyer J.E., Chakraborty T., Wehland J., Heinz D.W.
    J. Mol. Biol. 273:269-282(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 43-317.
    Strain: EGD / Serovar 1/2a.

Entry informationi

Entry nameiPLC_LISMO
AccessioniPrimary (citable) accession number: P34024
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 29, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3