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P34024 (PLC_LISMO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1-phosphatidylinositol phosphodiesterase

EC=4.6.1.13
Alternative name(s):
Phosphatidylinositol diacylglycerol-lyase
Phosphatidylinositol-specific phospholipase C
Short name=PI-PLC
Gene names
Name:plcA
Synonyms:pic
Ordered Locus Names:lmo0201
OrganismListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e) [Reference proteome] [HAMAP]
Taxonomic identifier169963 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria

Protein attributes

Sequence length317 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cleaves glycosylphosphatidylinositol (GPI) and phosphatidylinositol (PI) anchors but not PI phosphates. Important factor in pathogenesis, PI-PLC activity is present only in virulent listeria species. It may participate in the lysis of the phagolysosomal membrane.

Catalytic activity

1-phosphatidyl-1D-myo-inositol = 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol.

Subunit structure

Monomer.

Subcellular location

Secreted. Cytoplasm. Note: Secreted and, to a lesser extent, cytoplasmic.

Sequence similarities

Contains 1 PI-PLC X-box domain.

Ontologies

Keywords
   Biological processLipid degradation
Lipid metabolism
Virulence
   Cellular componentCytoplasm
Secreted
   DomainSignal
   Molecular functionLyase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processlipid catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

pathogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

extracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionphosphatidylinositol diacylglycerol-lyase activity

Inferred from electronic annotation. Source: UniProtKB-EC

phosphoric diester hydrolase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 3172951-phosphatidylinositol phosphodiesterase
PRO_0000024296

Regions

Domain58 – 196139PI-PLC X-box

Sites

Active site671Proton acceptor
Active site1151Proton donor

Secondary structure

................................................... 317
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P34024 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 5D0E33649A983DA2

FASTA31736,318
        10         20         30         40         50         60 
MYKNYLQRTL VLLLCFILYF FTFPLGGKAY SLNNWNKPIK NSVTTKQWMS ALPDTTNLAA 

        70         80         90        100        110        120 
LSIPGTHDTM SYNGDITWTL TKPLAQTQTM SLYQQLEAGI RYIDIRAKDN LNIYHGPIFL 

       130        140        150        160        170        180 
NASLSGVLET ITQFLKKNPK ETIIMRLKDE QNSNDSFDYR IQPLINIYKD YFYTTPRTDT 

       190        200        210        220        230        240 
SNKIPTLKDV RGKILLLSEN HTKKPLVINS RKFGMQFGAP NQVIQDDYNG PSVKTKFKEI 

       250        260        270        280        290        300 
VQTAYQASKA DNKLFLNHIS ATSLTFTPRQ YAAALNNKVE QFVLNLTSEK VRGLGILIMD 

       310 
FPEKQTIKNI IKNNKFN 

« Hide

References

« Hide 'large scale' references
[1]"Identification of phosphatidylinositol-specific phospholipase C activity in Listeria monocytogenes: a novel type of virulence factor?"
Mengaud J., Braun-Breton C., Cossart P.
Mol. Microbiol. 5:367-372(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: LO28 / Serovar 1/2c.
[2]"Detection of a gene encoding a phosphatidylinositol-specific phospholipase C that is co-ordinately expressed with listeriolysin in Listeria monocytogenes."
Leimeister-Waechter M., Domann E., Chakraborty T.
Mol. Microbiol. 5:361-366(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: EGD / Serovar 1/2a.
[3]"Comparative genomics of Listeria species."
Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. expand/collapse author list , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P.
Science 294:849-852(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-679 / EGD-e.
[4]"Transcriptional mapping and nucleotide sequence of the Listeria monocytogenes hlyA region reveal structural features that may be involved in regulation."
Mengaud J., Vicente M.-F., Cossart P.
Infect. Immun. 57:3695-3701(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 70-317.
[5]"Crystal structure of the phosphatidylinositol-specific phospholipase C from the human pathogen Listeria monocytogenes."
Moser J., Gerstel B., Meyer J.E., Chakraborty T., Wehland J., Heinz D.W.
J. Mol. Biol. 273:269-282(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 43-317.
Strain: EGD / Serovar 1/2a.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X54618 Genomic DNA. Translation: CAA38438.1.
AL591974 Genomic DNA. Translation: CAD00728.1.
PIRAB1100.
A37204. S15336.
RefSeqNP_463732.1. NC_003210.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1AODX-ray2.60A29-317[»]
2PLCX-ray2.00A43-316[»]
ProteinModelPortalP34024.
SMRP34024. Positions 43-316.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING169963.lmo0201.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD00728; CAD00728; CAD00728.
GeneID987032.
KEGGlmo:lmo0201.
PATRIC20309409. VBILisMon69206_0206.

Organism-specific databases

GenoListLMO0201.

Phylogenomic databases

eggNOGNOG14688.
KOK01771.
OMADLWIIPD.
OrthoDBEOG6091B5.

Enzyme and pathway databases

BioCycLMON169963:LMO0201-MONOMER.

Family and domain databases

Gene3D3.20.20.190. 1 hit.
InterProIPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
[Graphical view]
PfamPF00388. PI-PLC-X. 1 hit.
[Graphical view]
SMARTSM00148. PLCXc. 1 hit.
[Graphical view]
SUPFAMSSF51695. SSF51695. 1 hit.
PROSITEPS50007. PIPLC_X_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP34024.

Entry information

Entry namePLC_LISMO
AccessionPrimary (citable) accession number: P34024
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 14, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references