Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P34024

- PLC_LISMO

UniProt

P34024 - PLC_LISMO

Protein

1-phosphatidylinositol phosphodiesterase

Gene

plcA

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Cleaves glycosylphosphatidylinositol (GPI) and phosphatidylinositol (PI) anchors but not PI phosphates. Important factor in pathogenesis, PI-PLC activity is present only in virulent listeria species. It may participate in the lysis of the phagolysosomal membrane.

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol = 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei67 – 671Proton acceptor
    Active sitei115 – 1151Proton donor

    GO - Molecular functioni

    1. phosphatidylinositol diacylglycerol-lyase activity Source: UniProtKB-EC
    2. phosphoric diester hydrolase activity Source: InterPro

    GO - Biological processi

    1. lipid catabolic process Source: UniProtKB-KW
    2. pathogenesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism, Virulence

    Enzyme and pathway databases

    BioCyciLMON169963:LMO0201-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1-phosphatidylinositol phosphodiesterase (EC:4.6.1.13)
    Alternative name(s):
    Phosphatidylinositol diacylglycerol-lyase
    Phosphatidylinositol-specific phospholipase C
    Short name:
    PI-PLC
    Gene namesi
    Name:plcA
    Synonyms:pic
    Ordered Locus Names:lmo0201
    OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
    Taxonomic identifieri169963 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
    ProteomesiUP000000817: Chromosome

    Organism-specific databases

    GenoListiLMO0201.

    Subcellular locationi

    Secreted. Cytoplasm
    Note: Secreted and, to a lesser extent, cytoplasmic.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 3172951-phosphatidylinositol phosphodiesterasePRO_0000024296Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    STRINGi169963.lmo0201.

    Structurei

    Secondary structure

    1
    317
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi45 – 473
    Helixi49 – 513
    Helixi58 – 603
    Beta strandi61 – 666
    Turni67 – 726
    Helixi76 – 805
    Helixi82 – 854
    Helixi92 – 976
    Beta strandi102 – 1076
    Beta strandi111 – 1155
    Beta strandi118 – 1236
    Helixi124 – 13714
    Beta strandi143 – 1497
    Helixi156 – 16712
    Helixi168 – 1714
    Beta strandi174 – 1785
    Turni187 – 1926
    Beta strandi194 – 2007
    Beta strandi206 – 2083
    Beta strandi211 – 2166
    Beta strandi222 – 2254
    Helixi233 – 24917
    Beta strandi251 – 2588
    Beta strandi264 – 2663
    Helixi268 – 28821
    Beta strandi295 – 3017
    Helixi304 – 3118

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AODX-ray2.60A29-317[»]
    2PLCX-ray2.00A43-316[»]
    ProteinModelPortaliP34024.
    SMRiP34024. Positions 43-316.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP34024.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini58 – 196139PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG14688.
    KOiK01771.
    OMAiDLWIIPD.
    OrthoDBiEOG6091B5.

    Family and domain databases

    Gene3Di3.20.20.190. 1 hit.
    InterProiIPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    [Graphical view]
    PfamiPF00388. PI-PLC-X. 1 hit.
    [Graphical view]
    SMARTiSM00148. PLCXc. 1 hit.
    [Graphical view]
    SUPFAMiSSF51695. SSF51695. 1 hit.
    PROSITEiPS50007. PIPLC_X_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P34024-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYKNYLQRTL VLLLCFILYF FTFPLGGKAY SLNNWNKPIK NSVTTKQWMS    50
    ALPDTTNLAA LSIPGTHDTM SYNGDITWTL TKPLAQTQTM SLYQQLEAGI 100
    RYIDIRAKDN LNIYHGPIFL NASLSGVLET ITQFLKKNPK ETIIMRLKDE 150
    QNSNDSFDYR IQPLINIYKD YFYTTPRTDT SNKIPTLKDV RGKILLLSEN 200
    HTKKPLVINS RKFGMQFGAP NQVIQDDYNG PSVKTKFKEI VQTAYQASKA 250
    DNKLFLNHIS ATSLTFTPRQ YAAALNNKVE QFVLNLTSEK VRGLGILIMD 300
    FPEKQTIKNI IKNNKFN 317
    Length:317
    Mass (Da):36,318
    Last modified:February 1, 1994 - v1
    Checksum:i5D0E33649A983DA2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X54618 Genomic DNA. Translation: CAA38438.1.
    AL591974 Genomic DNA. Translation: CAD00728.1.
    PIRiAB1100.
    S15336. A37204.
    RefSeqiNP_463732.1. NC_003210.1.
    WP_003733144.1. NC_003210.1.

    Genome annotation databases

    EnsemblBacteriaiCAD00728; CAD00728; CAD00728.
    GeneIDi987032.
    KEGGilmo:lmo0201.
    PATRICi20309409. VBILisMon69206_0206.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X54618 Genomic DNA. Translation: CAA38438.1 .
    AL591974 Genomic DNA. Translation: CAD00728.1 .
    PIRi AB1100.
    S15336. A37204.
    RefSeqi NP_463732.1. NC_003210.1.
    WP_003733144.1. NC_003210.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AOD X-ray 2.60 A 29-317 [» ]
    2PLC X-ray 2.00 A 43-316 [» ]
    ProteinModelPortali P34024.
    SMRi P34024. Positions 43-316.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 169963.lmo0201.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAD00728 ; CAD00728 ; CAD00728 .
    GeneIDi 987032.
    KEGGi lmo:lmo0201.
    PATRICi 20309409. VBILisMon69206_0206.

    Organism-specific databases

    GenoListi LMO0201.

    Phylogenomic databases

    eggNOGi NOG14688.
    KOi K01771.
    OMAi DLWIIPD.
    OrthoDBi EOG6091B5.

    Enzyme and pathway databases

    BioCyci LMON169963:LMO0201-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P34024.

    Family and domain databases

    Gene3Di 3.20.20.190. 1 hit.
    InterProi IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    [Graphical view ]
    Pfami PF00388. PI-PLC-X. 1 hit.
    [Graphical view ]
    SMARTi SM00148. PLCXc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51695. SSF51695. 1 hit.
    PROSITEi PS50007. PIPLC_X_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of phosphatidylinositol-specific phospholipase C activity in Listeria monocytogenes: a novel type of virulence factor?"
      Mengaud J., Braun-Breton C., Cossart P.
      Mol. Microbiol. 5:367-372(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: LO28 / Serovar 1/2c.
    2. "Detection of a gene encoding a phosphatidylinositol-specific phospholipase C that is co-ordinately expressed with listeriolysin in Listeria monocytogenes."
      Leimeister-Waechter M., Domann E., Chakraborty T.
      Mol. Microbiol. 5:361-366(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: EGD / Serovar 1/2a.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-679 / EGD-e.
    4. "Transcriptional mapping and nucleotide sequence of the Listeria monocytogenes hlyA region reveal structural features that may be involved in regulation."
      Mengaud J., Vicente M.-F., Cossart P.
      Infect. Immun. 57:3695-3701(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 70-317.
    5. "Crystal structure of the phosphatidylinositol-specific phospholipase C from the human pathogen Listeria monocytogenes."
      Moser J., Gerstel B., Meyer J.E., Chakraborty T., Wehland J., Heinz D.W.
      J. Mol. Biol. 273:269-282(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 43-317.
      Strain: EGD / Serovar 1/2a.

    Entry informationi

    Entry nameiPLC_LISMO
    AccessioniPrimary (citable) accession number: P34024
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3