P34020 (LYS_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Autolytic lysozyme
EC=3.2.1.17

Alternative name(s):
1,4-beta-N-acetylmuramidase
Autolysin

Gene names

Name:	lyc
Ordered Locus Names:	CA_C0554

Organism

Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) [Reference proteome] [HAMAP]

Taxonomic identifier

272562 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium acetobutylicum

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Subunit structure	Monomer.
Subcellular location	Secreted. Cytoplasm.
Sequence similarities	Belongs to the glycosyl hydrolase 25 family.

Ontologies

Keywords
Cellular component	Cytoplasm Secreted
Domain	Repeat
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Autolytic lysozyme		PRO_0000208264
Regions
	Repeat	212 – 234	23	1
	Repeat	235 – 254	20	2
	Repeat	255 – 277	23	3
	Repeat	278 – 300	23	4
	Repeat	301 – 324	24	5
	Region	212 – 324	113	5 X 23 AA tandem repeats
Sites
	Active site	5	1	By similarity
	Active site	91	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P34020 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 32F09D6D77D015E9	FASTA	324	34,978
	10 20 30 40 50 60 MKGIDIYSGQ GSVDFNAVKE SGVEVVYIKA TEGLTYTDST YKDFYDGAKN AGLKIGFYHY 70 80 90 100 110 120 LRANDPTSEA EHFFNTISGL SLDCKCAIDV EVTLGQSIDQ ISSNVRKFAD YLINKGLDVC 130 140 150 160 170 180 VYTYTNFYKD NLNSTVKDLP LWIAEYGVSK PNIDASYVGF QYSDSGSVNG ISGSADLDEF 190 200 210 220 230 240 SEGILVGGTV VIDPGQGGDD NIKAIQQDLN ILLKRGLEVD GIEGPETEAA IKDFQSIMGL 250 260 270 280 290 300 TVDGIWGTNT SGAAQQIFSR PLDGVAYPHY EYATRYIQYR VGASVDGTFG SGTKAKVAAW 310 320 QSNQGLMADG VVGSATWSKL LDEN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of the lyc gene encoding the autolytic lysozyme of Clostridium acetobutylicum ATCC824: comparison with other lytic enzymes." Croux C., Garcia J.L. Gene 104:25-31(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[2]	"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[3]	"Purification and characterization of an extracellular muramidase of Clostridium acetobutylicum ATCC 824 that acts on non-N-acetylated peptidoglycan." Croux C., Canard B., Goma G., Soucaille P. Appl. Environ. Microbiol. 58:1075-1081(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-23, CHARACTERIZATION. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[4]	"Autolysis of Clostridium acetobutylicum ATCC 824." Croux C., Canard B., Goma G., Soucaille P. J. Gen. Microbiol. 138:861-869(1992) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M68865 Genomic DNA. Translation: AAA23250.1. AE001437 Genomic DNA. Translation: AAK78533.1.
PIR	B96968. JH0441.
RefSeq	NP_347193.1. NC_003030.1.
3D structure databases
ProteinModelPortal	P34020.
SMR	P34020. Positions 285-320.
ModBase	Search...
Protein-protein interaction databases
STRING	272562.CA_C0554.
Protein family/group databases
CAZy	GH25. Glycoside Hydrolase Family 25.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK78533; AAK78533; CA_C0554.
GeneID	1116737.
KEGG	cac:CA_C0554.
PATRIC	32035475. VBICloAce74127_0759.
Phylogenomic databases
eggNOG	COG3409.
HOGENOM	HOG000057939.
KO	K01185.
OMA	YGPASER.
ProtClustDB	CLSK801330.
Enzyme and pathway databases
BioCyc	CACE272562:GJIH-595-MONOMER.
Family and domain databases
Gene3D	1.10.101.10. 2 hits. 3.20.20.80. 1 hit.
InterPro	IPR002053. Glyco_hydro_25. IPR008270. Glyco_hydro_25_AS. IPR013781. Glyco_hydro_catalytic_dom. IPR018077. Glyco_hydro_fam25_subgr. IPR017853. Glycoside_hydrolase_SF. IPR002477. Peptidoglycan-bd-like. [Graphical view]
Pfam	PF01183. Glyco_hydro_25. 1 hit. PF01471. PG_binding_1. 2 hits. [Graphical view]
SMART	SM00641. Glyco_25. 2 hits. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF47090. PGBD_like. 2 hits.
PROSITE	PS00953. GLYCOSYL_HYDROL_F25. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYS_CLOAB

Accession

Primary (citable) accession number: P34020

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program