Reviewed,
UniProtKB/Swiss-Prot P34020 (LYS_CLOAB)
Last modified
June 16, 2009.
Version 82.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Autolytic lysozyme EC=3.2.1.17 Alternative name(s): 1,4-beta-N-acetylmuramidase Autolysin | ||||
| Gene names |
| ||||
| Organism | Clostridium acetobutylicum [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 1488 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium |
Protein attributes
| Sequence length | 324 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. |
| Subunit structure | Monomer. |
| Subcellular location | |
| Sequence similarities | Belongs to the glycosyl hydrolase 25 family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm Secreted |
| Domain | Repeat |
| Molecular function | Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase |
| Technical term | Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysisInferred from electronic annotation. Source: UniProtKB-KW defense response to bacteriumInferred from electronic annotation. Source: UniProtKB-KW peptidoglycan catabolic processInferred from electronic annotation. Source: InterPro |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular regionInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | cation binding Inferred from electronic annotation. Source: InterPro lysozyme activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 324 | 324 | Autolytic lysozyme | PRO_0000208264 | |||||
Regions | |||||||||
| Repeat | 212 – 234 | 23 | 1 | ||||||
| Repeat | 235 – 254 | 20 | 2 | ||||||
| Repeat | 255 – 277 | 23 | 3 | ||||||
| Repeat | 278 – 300 | 23 | 4 | ||||||
| Repeat | 301 – 324 | 24 | 5 | ||||||
| Region | 212 – 324 | 113 | 5 X 23 AA tandem repeats | ||||||
Sites | |||||||||
| Active site | 5 | 1 | By similarity | ||||||
| Active site | 91 | 1 | By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Sequence of the lyc gene encoding the autolytic lysozyme of Clostridium acetobutylicum ATCC824: comparison with other lytic enzymes." Croux C., Garcia J.L. Gene 104:25-31(1991) [PubMed: 1916274] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787. |
| [2] | "Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787. |
| [3] | "Purification and characterization of an extracellular muramidase of Clostridium acetobutylicum ATCC 824 that acts on non-N-acetylated peptidoglycan." Croux C., Canard B., Goma G., Soucaille P. Appl. Environ. Microbiol. 58:1075-1081(1992) [PubMed: 1599233] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-23, CHARACTERIZATION. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787. |
| [4] | "Autolysis of Clostridium acetobutylicum ATCC 824." Croux C., Canard B., Goma G., Soucaille P. J. Gen. Microbiol. 138:861-869(1992) [PubMed: 1645127] [Abstract] Cited for: CHARACTERIZATION. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787. |
Cross-references
Sequence databases | |
|---|---|
| M68865 Genomic DNA. Translation: AAA23250.1. AE001437 Genomic DNA. Translation: AAK78533.1. | |
| PIR | B96968. JH0441. |
| RefSeq | NP_347193.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1JFX based on UniProtKB P25310. |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | GH25. Glycoside Hydrolase Family 25. |
Genome annotation databases | |
| GeneID | 1116737. |
| GenomeReviews | Gene locus CA_C0554 in contig AE001437_GR. |
| KEGG | cac:CAC0554. |
| NMPDR | fig|272562.1.peg.722. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | P34020. |
| OMA | P34020. HYLRAND. |
Enzyme and pathway databases | |
| BioCyc | CACE272562:CAC0554-MON. |
| BRENDA | 3.2.1.17. 2866. |
Family and domain databases | |
| InterPro | IPR002053. Glyco_hydro_25. IPR008270. Glyco_hydro_25_AS. IPR018077. Glyco_hydro_fam25_sg. IPR013781. Glyco_hydro_sg_catalytic. IPR002477. Peptidoglycan-bd-like. [Graphical view] |
| Gene3D | G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:1.10.101.10. PGBD_1. 2 hits. |
| Pfam | PF01183. Glyco_hydro_25. 1 hit. PF01471. PG_binding_1. 2 hits. [Graphical view] |
| ProDom | PD004620. Glyco_hydro_25. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00641. Glyco_25. 2 hits. [Graphical view] |
| PROSITE | PS00953. GLYCOSYL_HYDROL_F25. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | LYS_CLOAB | ||||||||
| Accession | Primary (citable) accession number: P34020 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


