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P34020

- LYS_CLOAB

UniProt

P34020 - LYS_CLOAB

Protein

Autolytic lysozyme

Gene

lyc

Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei5 – 51PROSITE-ProRule annotation
    Active sitei91 – 911PROSITE-ProRule annotation

    GO - Molecular functioni

    1. lysozyme activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. cell wall macromolecule catabolic process Source: InterPro
    3. cytolysis Source: UniProtKB-KW
    4. defense response to bacterium Source: UniProtKB-KW
    5. peptidoglycan catabolic process Source: InterPro

    Keywords - Molecular functioni

    Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciCACE272562:GJIH-595-MONOMER.

    Protein family/group databases

    CAZyiGH25. Glycoside Hydrolase Family 25.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Autolytic lysozyme (EC:3.2.1.17)
    Alternative name(s):
    1,4-beta-N-acetylmuramidase
    Autolysin
    Gene namesi
    Name:lyc
    Ordered Locus Names:CA_C0554
    OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
    Taxonomic identifieri272562 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000000814: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 324324Autolytic lysozymePRO_0000208264Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    STRINGi272562.CA_C0554.

    Structurei

    3D structure databases

    ProteinModelPortaliP34020.
    SMRiP34020. Positions 285-320.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati212 – 234231Add
    BLAST
    Repeati235 – 254202Add
    BLAST
    Repeati255 – 277233Add
    BLAST
    Repeati278 – 300234Add
    BLAST
    Repeati301 – 324245Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni212 – 3241135 X 23 AA tandem repeatsAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 25 family.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG3409.
    HOGENOMiHOG000057939.
    KOiK01185.
    OMAiDITEVCL.
    OrthoDBiEOG6SV55S.

    Family and domain databases

    Gene3Di1.10.101.10. 2 hits.
    3.20.20.80. 1 hit.
    InterProiIPR002053. Glyco_hydro_25.
    IPR008270. Glyco_hydro_25_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR018077. Glyco_hydro_fam25_subgr.
    IPR017853. Glycoside_hydrolase_SF.
    IPR002477. Peptidoglycan-bd-like.
    [Graphical view]
    PfamiPF01183. Glyco_hydro_25. 1 hit.
    PF01471. PG_binding_1. 2 hits.
    [Graphical view]
    SMARTiSM00641. Glyco_25. 2 hits.
    [Graphical view]
    SUPFAMiSSF47090. SSF47090. 2 hits.
    SSF51445. SSF51445. 1 hit.
    PROSITEiPS00953. GLYCOSYL_HYDROL_F25. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P34020-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKGIDIYSGQ GSVDFNAVKE SGVEVVYIKA TEGLTYTDST YKDFYDGAKN    50
    AGLKIGFYHY LRANDPTSEA EHFFNTISGL SLDCKCAIDV EVTLGQSIDQ 100
    ISSNVRKFAD YLINKGLDVC VYTYTNFYKD NLNSTVKDLP LWIAEYGVSK 150
    PNIDASYVGF QYSDSGSVNG ISGSADLDEF SEGILVGGTV VIDPGQGGDD 200
    NIKAIQQDLN ILLKRGLEVD GIEGPETEAA IKDFQSIMGL TVDGIWGTNT 250
    SGAAQQIFSR PLDGVAYPHY EYATRYIQYR VGASVDGTFG SGTKAKVAAW 300
    QSNQGLMADG VVGSATWSKL LDEN 324
    Length:324
    Mass (Da):34,978
    Last modified:February 1, 1994 - v1
    Checksum:i32F09D6D77D015E9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M68865 Genomic DNA. Translation: AAA23250.1.
    AE001437 Genomic DNA. Translation: AAK78533.1.
    PIRiB96968.
    JH0441.
    RefSeqiNP_347193.1. NC_003030.1.
    WP_010963875.1. NC_003030.1.

    Genome annotation databases

    EnsemblBacteriaiAAK78533; AAK78533; CA_C0554.
    GeneIDi1116737.
    KEGGicac:CA_C0554.
    PATRICi32035475. VBICloAce74127_0759.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M68865 Genomic DNA. Translation: AAA23250.1 .
    AE001437 Genomic DNA. Translation: AAK78533.1 .
    PIRi B96968.
    JH0441.
    RefSeqi NP_347193.1. NC_003030.1.
    WP_010963875.1. NC_003030.1.

    3D structure databases

    ProteinModelPortali P34020.
    SMRi P34020. Positions 285-320.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272562.CA_C0554.

    Protein family/group databases

    CAZyi GH25. Glycoside Hydrolase Family 25.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK78533 ; AAK78533 ; CA_C0554 .
    GeneIDi 1116737.
    KEGGi cac:CA_C0554.
    PATRICi 32035475. VBICloAce74127_0759.

    Phylogenomic databases

    eggNOGi COG3409.
    HOGENOMi HOG000057939.
    KOi K01185.
    OMAi DITEVCL.
    OrthoDBi EOG6SV55S.

    Enzyme and pathway databases

    BioCyci CACE272562:GJIH-595-MONOMER.

    Family and domain databases

    Gene3Di 1.10.101.10. 2 hits.
    3.20.20.80. 1 hit.
    InterProi IPR002053. Glyco_hydro_25.
    IPR008270. Glyco_hydro_25_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR018077. Glyco_hydro_fam25_subgr.
    IPR017853. Glycoside_hydrolase_SF.
    IPR002477. Peptidoglycan-bd-like.
    [Graphical view ]
    Pfami PF01183. Glyco_hydro_25. 1 hit.
    PF01471. PG_binding_1. 2 hits.
    [Graphical view ]
    SMARTi SM00641. Glyco_25. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47090. SSF47090. 2 hits.
    SSF51445. SSF51445. 1 hit.
    PROSITEi PS00953. GLYCOSYL_HYDROL_F25. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of the lyc gene encoding the autolytic lysozyme of Clostridium acetobutylicum ATCC824: comparison with other lytic enzymes."
      Croux C., Garcia J.L.
      Gene 104:25-31(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
    3. "Purification and characterization of an extracellular muramidase of Clostridium acetobutylicum ATCC 824 that acts on non-N-acetylated peptidoglycan."
      Croux C., Canard B., Goma G., Soucaille P.
      Appl. Environ. Microbiol. 58:1075-1081(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-23, CHARACTERIZATION.
      Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
    4. "Autolysis of Clostridium acetobutylicum ATCC 824."
      Croux C., Canard B., Goma G., Soucaille P.
      J. Gen. Microbiol. 138:861-869(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

    Entry informationi

    Entry nameiLYS_CLOAB
    AccessioniPrimary (citable) accession number: P34020
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3