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P34020 (LYS_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Autolytic lysozyme

EC=3.2.1.17
Alternative name(s):
1,4-beta-N-acetylmuramidase
Autolysin
Gene names
Name:lyc
Ordered Locus Names:CA_C0554
OrganismClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) [Reference proteome] [HAMAP]
Taxonomic identifier272562 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length324 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

Subunit structure

Monomer.

Subcellular location

Secreted. Cytoplasm.

Sequence similarities

Belongs to the glycosyl hydrolase 25 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 324324Autolytic lysozyme
PRO_0000208264

Regions

Repeat212 – 234231
Repeat235 – 254202
Repeat255 – 277233
Repeat278 – 300234
Repeat301 – 324245
Region212 – 3241135 X 23 AA tandem repeats

Sites

Active site51 By similarity
Active site911 By similarity

Sequences

Sequence LengthMass (Da)Tools
P34020 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 32F09D6D77D015E9

FASTA32434,978
        10         20         30         40         50         60 
MKGIDIYSGQ GSVDFNAVKE SGVEVVYIKA TEGLTYTDST YKDFYDGAKN AGLKIGFYHY 

        70         80         90        100        110        120 
LRANDPTSEA EHFFNTISGL SLDCKCAIDV EVTLGQSIDQ ISSNVRKFAD YLINKGLDVC 

       130        140        150        160        170        180 
VYTYTNFYKD NLNSTVKDLP LWIAEYGVSK PNIDASYVGF QYSDSGSVNG ISGSADLDEF 

       190        200        210        220        230        240 
SEGILVGGTV VIDPGQGGDD NIKAIQQDLN ILLKRGLEVD GIEGPETEAA IKDFQSIMGL 

       250        260        270        280        290        300 
TVDGIWGTNT SGAAQQIFSR PLDGVAYPHY EYATRYIQYR VGASVDGTFG SGTKAKVAAW 

       310        320 
QSNQGLMADG VVGSATWSKL LDEN 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of the lyc gene encoding the autolytic lysozyme of Clostridium acetobutylicum ATCC824: comparison with other lytic enzymes."
Croux C., Garcia J.L.
Gene 104:25-31(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[2]"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum."
Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R.
J. Bacteriol. 183:4823-4838(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[3]"Purification and characterization of an extracellular muramidase of Clostridium acetobutylicum ATCC 824 that acts on non-N-acetylated peptidoglycan."
Croux C., Canard B., Goma G., Soucaille P.
Appl. Environ. Microbiol. 58:1075-1081(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-23, CHARACTERIZATION.
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[4]"Autolysis of Clostridium acetobutylicum ATCC 824."
Croux C., Canard B., Goma G., Soucaille P.
J. Gen. Microbiol. 138:861-869(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M68865 Genomic DNA. Translation: AAA23250.1.
AE001437 Genomic DNA. Translation: AAK78533.1.
PIRB96968.
JH0441.
RefSeqNP_347193.1. NC_003030.1.

3D structure databases

ProteinModelPortalP34020.
SMRP34020. Positions 285-320.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272562.CA_C0554.

Protein family/group databases

CAZyGH25. Glycoside Hydrolase Family 25.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK78533; AAK78533; CA_C0554.
GeneID1116737.
KEGGcac:CA_C0554.
PATRIC32035475. VBICloAce74127_0759.

Phylogenomic databases

eggNOGCOG3409.
HOGENOMHOG000057939.
KOK01185.
OMANANGCTG.
OrthoDBEOG6SV55S.
ProtClustDBCLSK801330.

Enzyme and pathway databases

BioCycCACE272562:GJIH-595-MONOMER.

Family and domain databases

Gene3D1.10.101.10. 2 hits.
3.20.20.80. 1 hit.
InterProIPR002053. Glyco_hydro_25.
IPR008270. Glyco_hydro_25_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR018077. Glyco_hydro_fam25_subgr.
IPR017853. Glycoside_hydrolase_SF.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PfamPF01183. Glyco_hydro_25. 1 hit.
PF01471. PG_binding_1. 2 hits.
[Graphical view]
SMARTSM00641. Glyco_25. 2 hits.
[Graphical view]
SUPFAMSSF47090. SSF47090. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEPS00953. GLYCOSYL_HYDROL_F25. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLYS_CLOAB
AccessionPrimary (citable) accession number: P34020
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 13, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries