Reviewed, UniProtKB/Swiss-Prot P34020 (LYS_CLOAB)

Names and origin

Protein names

Recommended name:
    Autolytic lysozyme
    EC=3.2.1.17
Alternative name(s):
    1,4-beta-N-acetylmuramidase
    Autolysin

Gene names

Name:	lyc
Ordered Locus Names:	CA_C0554

Organism

Clostridium acetobutylicum [Complete proteome] [HAMAP]

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Subunit structure	Monomer.
Subcellular location	Secreted. Cytoplasm.
Sequence similarities	Belongs to the glycosyl hydrolase 25 family.

Ontologies

Keywords
Cellular component	Cytoplasm Secreted
Domain	Repeat
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Autolytic lysozyme		PRO_0000208264
Regions
	Repeat	212 – 234	23	1
	Repeat	235 – 254	20	2
	Repeat	255 – 277	23	3
	Repeat	278 – 300	23	4
	Repeat	301 – 324	24	5
	Region	212 – 324	113	5 X 23 AA tandem repeats
Sites
	Active site	5	1	By similarity
	Active site	91	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P34020-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 32F09D6D77D015E9	FASTA	324	34,978
	10 20 30 40 50 60 MKGIDIYSGQ GSVDFNAVKE SGVEVVYIKA TEGLTYTDST YKDFYDGAKN AGLKIGFYHY 70 80 90 100 110 120 LRANDPTSEA EHFFNTISGL SLDCKCAIDV EVTLGQSIDQ ISSNVRKFAD YLINKGLDVC 130 140 150 160 170 180 VYTYTNFYKD NLNSTVKDLP LWIAEYGVSK PNIDASYVGF QYSDSGSVNG ISGSADLDEF 190 200 210 220 230 240 SEGILVGGTV VIDPGQGGDD NIKAIQQDLN ILLKRGLEVD GIEGPETEAA IKDFQSIMGL 250 260 270 280 290 300 TVDGIWGTNT SGAAQQIFSR PLDGVAYPHY EYATRYIQYR VGASVDGTFG SGTKAKVAAW 310 320 QSNQGLMADG VVGSATWSKL LDEN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of the lyc gene encoding the autolytic lysozyme of Clostridium acetobutylicum ATCC824: comparison with other lytic enzymes." Croux C., Garcia J.L. Gene 104:25-31(1991) [PubMed: 1916274] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[2]	"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[3]	"Purification and characterization of an extracellular muramidase of Clostridium acetobutylicum ATCC 824 that acts on non-N-acetylated peptidoglycan." Croux C., Canard B., Goma G., Soucaille P. Appl. Environ. Microbiol. 58:1075-1081(1992) [PubMed: 1599233] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-23, CHARACTERIZATION. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[4]	"Autolysis of Clostridium acetobutylicum ATCC 824." Croux C., Canard B., Goma G., Soucaille P. J. Gen. Microbiol. 138:861-869(1992) [PubMed: 1645127] [Abstract] Cited for: CHARACTERIZATION. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M68865 Genomic DNA. Translation: AAA23250.1. AE001437 Genomic DNA. Translation: AAK78533.1.
PIR	B96968. JH0441.
RefSeq	NP_347193.1.
3D structure databases
HSSP	HSSP built from PDB template 1JFX based on UniProtKB P25310.
ModBase	Search...
Protein family/group databases
CAZy	GH25. Glycoside Hydrolase Family 25.
Genome annotation databases
GeneID	1116737.
GenomeReviews	Gene locus CA_C0554 in contig AE001437_GR.
KEGG	cac:CAC0554.
NMPDR	fig|272562.1.peg.722.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P34020.
OMA	P34020. HYLRAND.
Enzyme and pathway databases
BioCyc	CACE272562:CAC0554-MON.
BRENDA	3.2.1.17. 2866.
Family and domain databases
InterPro	IPR002053. Glyco_hydro_25. IPR008270. Glyco_hydro_25_AS. IPR018077. Glyco_hydro_fam25_sg. IPR013781. Glyco_hydro_sg_catalytic. IPR002477. Peptidoglycan-bd-like. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:1.10.101.10. PGBD_1. 2 hits.
Pfam	PF01183. Glyco_hydro_25. 1 hit. PF01471. PG_binding_1. 2 hits. [Graphical view]
ProDom	PD004620. Glyco_hydro_25. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00641. Glyco_25. 2 hits. [Graphical view]
PROSITE	PS00953. GLYCOSYL_HYDROL_F25. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYS_CLOAB

Accession

Primary (citable) accession number: P34020

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)