Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Autolytic lysozyme

Gene

lyc

Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei5 – 51PROSITE-ProRule annotation
Active sitei91 – 911PROSITE-ProRule annotation

GO - Molecular functioni

  1. lysozyme activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. cell wall macromolecule catabolic process Source: InterPro
  3. cytolysis Source: UniProtKB-KW
  4. defense response to bacterium Source: UniProtKB-KW
  5. peptidoglycan catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciCACE272562:GJIH-595-MONOMER.

Protein family/group databases

CAZyiGH25. Glycoside Hydrolase Family 25.

Names & Taxonomyi

Protein namesi
Recommended name:
Autolytic lysozyme (EC:3.2.1.17)
Alternative name(s):
1,4-beta-N-acetylmuramidase
Autolysin
Gene namesi
Name:lyc
Ordered Locus Names:CA_C0554
OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Taxonomic identifieri272562 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000814 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 324324Autolytic lysozymePRO_0000208264Add
BLAST

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi272562.CA_C0554.

Structurei

3D structure databases

ProteinModelPortaliP34020.
SMRiP34020. Positions 285-320.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati212 – 234231Add
BLAST
Repeati235 – 254202Add
BLAST
Repeati255 – 277233Add
BLAST
Repeati278 – 300234Add
BLAST
Repeati301 – 324245Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni212 – 3241135 X 23 AA tandem repeatsAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 25 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG3409.
HOGENOMiHOG000057939.
KOiK01185.
OMAiYEGDISG.
OrthoDBiEOG6SV55S.

Family and domain databases

Gene3Di1.10.101.10. 2 hits.
3.20.20.80. 1 hit.
InterProiIPR002053. Glyco_hydro_25.
IPR008270. Glyco_hydro_25_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR018077. Glyco_hydro_fam25_subgr.
IPR017853. Glycoside_hydrolase_SF.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PfamiPF01183. Glyco_hydro_25. 1 hit.
PF01471. PG_binding_1. 2 hits.
[Graphical view]
SMARTiSM00641. Glyco_25. 2 hits.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00953. GLYCOSYL_HYDROL_F25. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34020-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGIDIYSGQ GSVDFNAVKE SGVEVVYIKA TEGLTYTDST YKDFYDGAKN
60 70 80 90 100
AGLKIGFYHY LRANDPTSEA EHFFNTISGL SLDCKCAIDV EVTLGQSIDQ
110 120 130 140 150
ISSNVRKFAD YLINKGLDVC VYTYTNFYKD NLNSTVKDLP LWIAEYGVSK
160 170 180 190 200
PNIDASYVGF QYSDSGSVNG ISGSADLDEF SEGILVGGTV VIDPGQGGDD
210 220 230 240 250
NIKAIQQDLN ILLKRGLEVD GIEGPETEAA IKDFQSIMGL TVDGIWGTNT
260 270 280 290 300
SGAAQQIFSR PLDGVAYPHY EYATRYIQYR VGASVDGTFG SGTKAKVAAW
310 320
QSNQGLMADG VVGSATWSKL LDEN
Length:324
Mass (Da):34,978
Last modified:January 31, 1994 - v1
Checksum:i32F09D6D77D015E9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M68865 Genomic DNA. Translation: AAA23250.1.
AE001437 Genomic DNA. Translation: AAK78533.1.
PIRiB96968.
JH0441.
RefSeqiNP_347193.1. NC_003030.1.
WP_010963875.1. NC_003030.1.

Genome annotation databases

EnsemblBacteriaiAAK78533; AAK78533; CA_C0554.
GeneIDi1116737.
KEGGicac:CA_C0554.
PATRICi32035475. VBICloAce74127_0759.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M68865 Genomic DNA. Translation: AAA23250.1.
AE001437 Genomic DNA. Translation: AAK78533.1.
PIRiB96968.
JH0441.
RefSeqiNP_347193.1. NC_003030.1.
WP_010963875.1. NC_003030.1.

3D structure databases

ProteinModelPortaliP34020.
SMRiP34020. Positions 285-320.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272562.CA_C0554.

Protein family/group databases

CAZyiGH25. Glycoside Hydrolase Family 25.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK78533; AAK78533; CA_C0554.
GeneIDi1116737.
KEGGicac:CA_C0554.
PATRICi32035475. VBICloAce74127_0759.

Phylogenomic databases

eggNOGiCOG3409.
HOGENOMiHOG000057939.
KOiK01185.
OMAiYEGDISG.
OrthoDBiEOG6SV55S.

Enzyme and pathway databases

BioCyciCACE272562:GJIH-595-MONOMER.

Family and domain databases

Gene3Di1.10.101.10. 2 hits.
3.20.20.80. 1 hit.
InterProiIPR002053. Glyco_hydro_25.
IPR008270. Glyco_hydro_25_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR018077. Glyco_hydro_fam25_subgr.
IPR017853. Glycoside_hydrolase_SF.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PfamiPF01183. Glyco_hydro_25. 1 hit.
PF01471. PG_binding_1. 2 hits.
[Graphical view]
SMARTiSM00641. Glyco_25. 2 hits.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00953. GLYCOSYL_HYDROL_F25. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of the lyc gene encoding the autolytic lysozyme of Clostridium acetobutylicum ATCC824: comparison with other lytic enzymes."
    Croux C., Garcia J.L.
    Gene 104:25-31(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
  3. "Purification and characterization of an extracellular muramidase of Clostridium acetobutylicum ATCC 824 that acts on non-N-acetylated peptidoglycan."
    Croux C., Canard B., Goma G., Soucaille P.
    Appl. Environ. Microbiol. 58:1075-1081(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-23, CHARACTERIZATION.
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
  4. "Autolysis of Clostridium acetobutylicum ATCC 824."
    Croux C., Canard B., Goma G., Soucaille P.
    J. Gen. Microbiol. 138:861-869(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Entry informationi

Entry nameiLYS_CLOAB
AccessioniPrimary (citable) accession number: P34020
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 1994
Last sequence update: January 31, 1994
Last modified: January 6, 2015
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.