P34003 (AROK_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Shikimate kinase
Short name=SK
EC=2.7.1.71

Gene names

Name:	aroK
Ordered Locus Names:	PA5039

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa

Protein attributes

Sequence length	172 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate By similarity. HAMAP-Rule MF_00109
Catalytic activity	ATP + shikimate = ADP + shikimate 3-phosphate. HAMAP-Rule MF_00109
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7. HAMAP-Rule MF_00109
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the shikimate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP chorismate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP shikimate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 172	172	Shikimate kinase HAMAP-Rule MF_00109		PRO_0000192400
Regions
	Nucleotide binding	11 – 16	6	ATP By similarity
Sites
	Metal binding	15	1	Magnesium By similarity
	Binding site	33	1	Substrate By similarity
	Binding site	57	1	Substrate By similarity
	Binding site	79	1	Substrate; via amide nitrogen By similarity
	Binding site	117	1	ATP By similarity
	Binding site	136	1	Substrate By similarity
	Binding site	153	1	ATP By similarity
Experimental info
	Sequence conflict	59	1	R → L in AAA16705. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P34003 [UniParc]. Last modified December 8, 2000. Version 2. Checksum: 1C4C188D8BA2B5DD	FASTA	172	19,237
	10 20 30 40 50 60 MVNLILVGPM GAGKSTIGRL LAKELHLAFK DSDKEIEQRC GANIPWIFDV EGEVGFRERE 70 80 90 100 110 120 QAMLTELCAA DGMVIATGGG AVMRDGNRQV LRAGGRVVYL HASVEHQIAR TARDRNRPLL 130 140 150 160 170 QKPNPGQILR DLMALRDPLY REIADVVVET DERPPRLVVQ EILERLRKLP PR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Characterization of pilQ, a new gene required for the biogenesis of type 4 fimbriae in Pseudomonas aeruginosa." Martin P.R., Hobbs M., Free P.D., Jeske Y., Mattick J.S. Mol. Microbiol. 9:857-868(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-60. Strain: ATCC 25102 / PAK.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG08424.1. L13865 Unassigned DNA. Translation: AAA16705.1.
PIR	H83015. S38833.
RefSeq	NP_253726.1. NC_002516.2.
3D structure databases
ProteinModelPortal	P34003.
SMR	P34003. Positions 3-148.
ModBase	Search...
Protein-protein interaction databases
STRING	208964.PA5039.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	880961.
KEGG	pae:PA5039.
PATRIC	19844909. VBIPseAer58763_5283.
Organism-specific databases
PseudoCAP	PA5039.
Phylogenomic databases
eggNOG	COG0703.
HOGENOM	HOG000032568.
KO	K00891.
OMA	ADIPWIF.
ProtClustDB	PRK00131.
Enzyme and pathway databases
UniPathway	UPA00053; UER00088.
Family and domain databases
HAMAP	MF_00109. Shikimate_kinase.
InterPro	IPR000623. Shikimate_kinase. IPR023000. Shikimate_kinase_CS. [Graphical view]
Pfam	PF01202. SKI. 1 hit. [Graphical view]
PRINTS	PR01100. SHIKIMTKNASE.
PROSITE	PS01128. SHIKIMATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROK_PSEAE

Accession

Primary (citable) accession number: P34003

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	December 8, 2000
Last modified:	May 1, 2013
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program