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Protein

DNA-binding transcriptional activator AlpA

Gene

alpA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein predictedi

Functioni

Positive regulator of the expression of the slpA gene (PubMed:7511582). When overexpressed, leads to suppression of the capsule overproduction and UV sensitivity phenotypes of cells mutant for the Lon ATP-dependent protease (PubMed:7511582). Part of the cryptic P4-like prophage CP4-57 (PubMed:7511583). Overexpression of AlpA leads to excision of the CP4-57 prophage by IntA. This inactivates ssrA (the gene upstream of the prophage) that encodes tmRNA which is required to rescue stalled ribosomes in a process known as trans-translation (PubMed:7511583).2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi12 – 31H-T-H motifSequence analysisAdd BLAST20

GO - Molecular functioni

GO - Biological processi

  • positive regulation of transcription, DNA-templated Source: EcoCyc
  • single-species biofilm formation Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:PD02850.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding transcriptional activator AlpACurated
Alternative name(s):
Prophage CP4-57 regulatory protein AlpA
Gene namesi
Name:alpA1 Publication
Synonyms:alp
Ordered Locus Names:b2624, JW2604
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12112. alpA.

Subcellular locationi

GO - Cellular componenti

  • provirus Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000645711 – 70DNA-binding transcriptional activator AlpAAdd BLAST70

Proteomic databases

PaxDbiP33997.
PRIDEiP33997.

Interactioni

Protein-protein interaction databases

BioGridi4260621. 8 interactors.
IntActiP33997. 3 interactors.
STRINGi511145.b2624.

Structurei

3D structure databases

ProteinModelPortaliP33997.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiENOG410679B. Bacteria.
COG3311. LUCA.
HOGENOMiHOG000263166.
InParanoidiP33997.
KOiK07733.
OMAiGWLESEI.

Family and domain databases

InterProiView protein in InterPro
IPR010260. Phage_AlpA.
PfamiView protein in Pfam
PF05930. Phage_AlpA. 1 hit.

Sequencei

Sequence statusi: Complete.

P33997-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNRSAVRIL RLPAVIQKTG MARATIYDWL NPKSPRYDAT FPKKRMLGVK
60 70
SVGWIEAEID EWLSQRCKLI
Length:70
Mass (Da):8,133
Last modified:February 1, 1994 - v1
Checksum:i85E3E99885A1EF4C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03737 Unassigned DNA. Translation: AAA18418.1.
U36840 Genomic DNA. Translation: AAA79793.1.
U00096 Genomic DNA. Translation: AAC75672.1.
AP009048 Genomic DNA. Translation: BAE76759.1.
PIRiB65041.
RefSeqiNP_417113.1. NC_000913.3.
WP_001065343.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75672; AAC75672; b2624.
BAE76759; BAE76759; BAE76759.
GeneIDi946758.
KEGGiecj:JW2604.
eco:b2624.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03737 Unassigned DNA. Translation: AAA18418.1.
U36840 Genomic DNA. Translation: AAA79793.1.
U00096 Genomic DNA. Translation: AAC75672.1.
AP009048 Genomic DNA. Translation: BAE76759.1.
PIRiB65041.
RefSeqiNP_417113.1. NC_000913.3.
WP_001065343.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP33997.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260621. 8 interactors.
IntActiP33997. 3 interactors.
STRINGi511145.b2624.

Proteomic databases

PaxDbiP33997.
PRIDEiP33997.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75672; AAC75672; b2624.
BAE76759; BAE76759; BAE76759.
GeneIDi946758.
KEGGiecj:JW2604.
eco:b2624.

Organism-specific databases

EchoBASEiEB2035.
EcoGeneiEG12112. alpA.

Phylogenomic databases

eggNOGiENOG410679B. Bacteria.
COG3311. LUCA.
HOGENOMiHOG000263166.
InParanoidiP33997.
KOiK07733.
OMAiGWLESEI.

Enzyme and pathway databases

BioCyciEcoCyc:PD02850.

Miscellaneous databases

PROiP33997.

Family and domain databases

InterProiView protein in InterPro
IPR010260. Phage_AlpA.
PfamiView protein in Pfam
PF05930. Phage_AlpA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiALPA_ECOLI
AccessioniPrimary (citable) accession number: P33997
Secondary accession number(s): Q2MAE7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: March 15, 2017
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.