Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA replication licensing factor MCM5

Gene

MCM5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity). Interacts with MCMBP.By similarity

Miscellaneous

Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. The MCM2-7 hexamer is the proposed physiological active complex.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi381 – 388ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processCell cycle, DNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-176187. Activation of ATR in response to replication stress.
R-HSA-176974. Unwinding of DNA.
R-HSA-68867. Assembly of the pre-replicative complex.
R-HSA-68949. Orc1 removal from chromatin.
R-HSA-68962. Activation of the pre-replicative complex.
R-HSA-69052. Switching of origins to a post-replicative state.
R-HSA-69300. Removal of licensing factors from origins.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM5 (EC:3.6.4.12)
Alternative name(s):
CDC46 homolog
P1-CDC46
Gene namesi
Name:MCM5
Synonyms:CDC46
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

HGNCiHGNC:6948. MCM5.

Subcellular locationi

GO - Cellular componenti

  • MCM complex Source: UniProtKB
  • membrane Source: UniProtKB
  • nuclear chromosome, telomeric region Source: BHF-UCL
  • nucleoplasm Source: HPA
  • nucleus Source: ProtInc

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi4174.
OpenTargetsiENSG00000100297.
PharmGKBiPA30695.

Polymorphism and mutation databases

BioMutaiMCM5.
DMDMi19858646.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001941072 – 734DNA replication licensing factor MCM5Add BLAST733

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei315PhosphoserineCombined sources1
Modified residuei392N6-acetyllysineCombined sources1
Modified residuei396N6-acetyllysineCombined sources1
Modified residuei605PhosphoserineCombined sources1
Modified residuei696N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP33992.
MaxQBiP33992.
PaxDbiP33992.
PeptideAtlasiP33992.
PRIDEiP33992.

PTM databases

iPTMnetiP33992.
PhosphoSitePlusiP33992.
SwissPalmiP33992.

Miscellaneous databases

PMAP-CutDBiP33992.

Expressioni

Gene expression databases

BgeeiENSG00000100297.
CleanExiHS_MCM5.
ExpressionAtlasiP33992. baseline and differential.
GenevisibleiP33992. HS.

Organism-specific databases

HPAiCAB000101.
HPA000845.
HPA052880.

Interactioni

Subunit structurei

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (Probable). Interacts with ANKRD17.Curated3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi110342. 236 interactors.
DIPiDIP-27578N.
IntActiP33992. 54 interactors.
MINTiMINT-5004198.
STRINGi9606.ENSP00000216122.

Structurei

3D structure databases

ProteinModelPortaliP33992.
SMRiP33992.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini331 – 537MCMAdd BLAST207

Sequence similaritiesi

Belongs to the MCM family.Curated

Phylogenomic databases

eggNOGiKOG0481. Eukaryota.
COG1241. LUCA.
GeneTreeiENSGT00550000074928.
HOGENOMiHOG000224128.
HOVERGENiHBG104907.
InParanoidiP33992.
KOiK02209.
OMAiIHKVLQL.
OrthoDBiEOG091G027N.
PhylomeDBiP33992.
TreeFamiTF105653.

Family and domain databases

InterProiView protein in InterPro
IPR031327. MCM.
IPR008048. MCM5.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR033762. MCM_OB.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
PANTHERiPTHR11630:SF87. PTHR11630:SF87. 1 hit.
PfamiView protein in Pfam
PF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
PF17207. MCM_OB. 1 hit.
PRINTSiPR01657. MCMFAMILY.
PR01661. MCMPROTEIN5.
SMARTiView protein in SMART
SM00350. MCM. 1 hit.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33992-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGFDDPGIF YSDSFGGDAQ ADEGQARKSQ LQRRFKEFLR QYRVGTDRTG
60 70 80 90 100
FTFKYRDELK RHYNLGEYWI EVEMEDLASF DEDLADYLYK QPAEHLQLLE
110 120 130 140 150
EAAKEVADEV TRPRPSGEEV LQDIQVMLKS DASPSSIRSL KSDMMSHLVK
160 170 180 190 200
IPGIIIAASA VRAKATRISI QCRSCRNTLT NIAMRPGLEG YALPRKCNTD
210 220 230 240 250
QAGRPKCPLD PYFIMPDKCK CVDFQTLKLQ ELPDAVPHGE MPRHMQLYCD
260 270 280 290 300
RYLCDKVVPG NRVTIMGIYS IKKFGLTTSR GRDRVGVGIR SSYIRVLGIQ
310 320 330 340 350
VDTDGSGRSF AGAVSPQEEE EFRRLAALPN VYEVISKSIA PSIFGGTDMK
360 370 380 390 400
KAIACLLFGG SRKRLPDGLT RRGDINLLML GDPGTAKSQL LKFVEKCSPI
410 420 430 440 450
GVYTSGKGSS AAGLTASVMR DPSSRNFIME GGAMVLADGG VVCIDEFDKM
460 470 480 490 500
REDDRVAIHE AMEQQTISIA KAGITTTLNS RCSVLAAANS VFGRWDETKG
510 520 530 540 550
EDNIDFMPTI LSRFDMIFIV KDEHNEERDV MLAKHVITLH VSALTQTQAV
560 570 580 590 600
EGEIDLAKLK KFIAYCRVKC GPRLSAEAAE KLKNRYIIMR SGARQHERDS
610 620 630 640 650
DRRSSIPITV RQLEAIVRIA EALSKMKLQP FATEADVEEA LRLFQVSTLD
660 670 680 690 700
AALSGTLSGV EGFTSQEDQE MLSRIEKQLK RRFAIGSQVS EHSIIKDFTK
710 720 730
QKYPEHAIHK VLQLMLRRGE IQHRMQRKVL YRLK
Length:734
Mass (Da):82,286
Last modified:August 14, 2001 - v5
Checksum:iA80280E61749998D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41Q → R in AAH03656 (PubMed:15489334).Curated1
Sequence conflicti434M → W (PubMed:8265339).Curated1
Sequence conflicti527E → V in BAA12176 (Ref. 3) Curated1
Sequence conflicti591S → T in BAA12176 (Ref. 3) Curated1
Sequence conflicti593 – 603ARQHERDSDRR → PVSTRGTVTA in BAA12176 (Ref. 3) CuratedAdd BLAST11

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_014813136S → T1 PublicationCorresponds to variant dbSNP:rs2307334Ensembl.1
Natural variantiVAR_014814180T → S1 PublicationCorresponds to variant dbSNP:rs2307340Ensembl.1
Natural variantiVAR_014815258V → I1 PublicationCorresponds to variant dbSNP:rs2230933Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74795 mRNA. Translation: CAA52802.2.
D83986 mRNA. Translation: BAA12176.1.
CR456517 mRNA. Translation: CAG30403.1.
AY212028 Genomic DNA. Translation: AAO21127.1.
Z82244 Genomic DNA. No translation available.
BC000142 mRNA. Translation: AAH00142.1.
BC003656 mRNA. Translation: AAH03656.1.
CCDSiCCDS13915.1.
PIRiI38080.
RefSeqiNP_006730.2. NM_006739.3.
UniGeneiHs.517582.

Genome annotation databases

EnsembliENST00000216122; ENSP00000216122; ENSG00000100297.
GeneIDi4174.
KEGGihsa:4174.
UCSCiuc003anu.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiMCM5_HUMAN
AccessioniPrimary (citable) accession number: P33992
Secondary accession number(s): O60785
, Q14578, Q9BTJ4, Q9BWL8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: August 14, 2001
Last modified: August 30, 2017
This is version 187 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families