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P33990 (SQHC_ZYMMO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Squalene--hopene cyclase
EC=4.2.1.129
EC=5.4.99.17
Alternative name(s):
Squalene--hopanol cyclase
Gene names
Name:shc
Ordered Locus Names:ZMO0872
OrganismZymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) [Complete proteome] [HAMAP]
Taxonomic identifier264203 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Zymomonas ›

Protein attributes

Sequence length658 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the cyclization of squalene into hopene.

Catalytic activity

Squalene = hop-22(29)-ene.

Hopan-22-ol = squalene + H2O.

Pathway

Secondary metabolite biosynthesis; hopanoid biosynthesis.

Subcellular location

Cell membrane; Peripheral membrane protein By similarity.

Sequence similarities

Belongs to the terpene cyclase/mutase family.

Contains 4 PFTB repeats.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainRepeat
   Molecular functionIsomerase
Lyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processhopanoid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionlyase activity

Inferred from electronic annotation. Source: UniProtKB-KW

squalene-hopene cyclase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 658658Squalene--hopene cyclase
PRO_0000072653

Regions

Repeat69 – 11042PFTB 1
Repeat418 – 45942PFTB 2
Repeat486 – 52641PFTB 3
Repeat534 – 58451PFTB 4

Experimental info

Sequence conflict341H → Q Ref.1
Sequence conflict341H → Q Ref.2
Sequence conflict341H → Q Ref.3
Sequence conflict641Q → E Ref.1
Sequence conflict641Q → E Ref.2
Sequence conflict641Q → E Ref.3
Sequence conflict4111R → H Ref.1
Sequence conflict4111R → H Ref.2
Sequence conflict4111R → H Ref.3
Sequence conflict5341I → V Ref.1
Sequence conflict5341I → V Ref.2
Sequence conflict5341I → V Ref.3

Sequences

Sequence LengthMass (Da)Tools
P33990 [UniParc].

Last modified February 15, 2005. Version 2.
Checksum: 42D2D8FC06EA1D9C

FASTA65874,109
        10         20         30         40         50         60 
MTVSTSSAFH HSPLSDDVEP IIQKATRALL EKQHQDGHWV FELEADATIP AEYILLKHYL 

        70         80         90        100        110        120 
GEPQDLEIEA KIGRYLRRIQ GEHGGWSLFY GGDLDLSATV KAYFALKMIG DSPDAPHMLR 

       130        140        150        160        170        180 
ARNEILARGG AMRANVFTRI QLALFGAMSW EHVPQMPVEL MLMPEWFPVH INKMAYWART 

       190        200        210        220        230        240 
VLVPLLVLQA LKPVARNRRG ILVDELFVPD VLPTLQESGD PIWRRFFSAL DKVLHKVEPY 

       250        260        270        280        290        300 
WPKNMRAKAI HSCVHFVTER LNGEDGLGAI YPAIANSVMM YDALGYPENH PERAIARRAV 

       310        320        330        340        350        360 
EKLMVLDGTE DQGDKEVYCQ PCLSPIWDTA LVAHAMLEVG GDEAEKSAIS ALSWLKPQQI 

       370        380        390        400        410        420 
LDVKGDWAWR RPDLRPGGWA FQYRNDYYPD VDDTAVVTMA MDRAAKLSDL RDDFEESKAR 

       430        440        450        460        470        480 
AMEWTIGMQS DNGGWGAFDA NNSYTYLNNI PFADHGALLD PPTVDVSARC VSMMAQAGIS 

       490        500        510        520        530        540 
ITDPKMKAAV DYLLKEQEED GSWFGRWGVN YIYGTWSALC ALNVAALPHD HLAIQKAVAW 

       550        560        570        580        590        600 
LKTIQNEDGG WGENCDSYAL DYSGYEPMDS TASQTAWALL GLMAVGEANS EAVTKGINWL 

       610        620        630        640        650 
AQNQDEEGLW KEDYYSGGGF PRVFYLRYHG YSKYFPLWAL ARYRNLKKAN QPIVHYGM

« Hide

References

« Hide 'large scale' references
[1]"Zymomonas mobilis squalene-hopene cyclase gene (shc): cloning, DNA sequence analysis, and expression in Escherichia coli."
Reipen I.G., Poralla K., Sahm H., Sprenger G.A.
Microbiology 141:155-161(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 31821 / ZM4 / CP4.
[2]Reipen I.G., Sahm H., Sprenger G.A.
Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 31821 / ZM4 / CP4.
[3]Um H.W.
Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 31821 / ZM4 / CP4.
[4]"The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4."
Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H., Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J., Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y. expand/collapse author list , Kang H.L., Lee S.Y., Lee K.J., Kang H.S.
Nat. Biotechnol. 23:63-68(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 31821 / ZM4 / CP4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X80766 Genomic DNA. Translation: CAA56749.1.
X73561 Genomic DNA. Translation: CAA51958.1.
AJ001401 Genomic DNA. Translation: CAA04735.1.
AF203881 Genomic DNA. Translation: AAF12829.1.
AE008692 Genomic DNA. Translation: AAV89496.1.
PIRS37494.
RefSeqYP_162607.1. NC_006526.2.

3D structure databases

ProteinModelPortalP33990.
ModBaseSearch...

Protein-protein interaction databases

STRING264203.ZMO0872.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAV89496; AAV89496; ZMO0872.
GeneID3187471.
KEGGzmo:ZMO0872.
PATRIC32567038. VBIZymMob102260_0823.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1657.
HOGENOMHOG000220823.
KOK06045.
OMARRIQGAH.
ProtClustDBCLSK2524696.

Enzyme and pathway databases

UniPathwayUPA00337.

Family and domain databases

InterProIPR006400. Hopene-cyclase.
IPR001330. Prenyltrans.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamPF00432. Prenyltrans. 3 hits.
[Graphical view]
SUPFAMSSF48239. Terp_cyc_toroid. 2 hits.
TIGRFAMsTIGR01507. hopene_cyclase. 1 hit.
TIGR01787. squalene_cyclas. 1 hit.
PROSITEPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSQHC_ZYMMO
AccessionPrimary (citable) accession number: P33990
Secondary accession number(s): Q5NP64
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 15, 2005
Last modified: May 1, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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