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Protein

RNA polymerase sigma-54 factor

Gene

rpoN

Organism
Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi391 – 410H-T-H motifSequence analysisAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Nucleotidyltransferase, Sigma factor, Transferase
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciACAU438753:GJF3-3970-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase sigma-54 factor
Gene namesi
Name:rpoN
Synonyms:ntrA
Ordered Locus Names:AZC_3925
OrganismiAzorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)
Taxonomic identifieri438753 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeAzorhizobium
Proteomesi
  • UP000000270 Componenti: Chromosome

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002055221 – 514RNA polymerase sigma-54 factorAdd BLAST514

Proteomic databases

PRIDEiP33984.

Interactioni

Protein-protein interaction databases

STRINGi438753.AZC_3925.

Structurei

3D structure databases

ProteinModelPortaliP33984.
SMRiP33984.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi480 – 488RPON box9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi11 – 29Gln-richAdd BLAST19

Sequence similaritiesi

Belongs to the sigma-54 factor family.Curated

Phylogenomic databases

eggNOGiENOG4105DTW. Bacteria.
COG1508. LUCA.
HOGENOMiHOG000071904.
KOiK03092.
OMAiVTTQKFM.
OrthoDBiPOG091H02EW.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR000394. RNA_pol_sigma_54.
IPR007046. RNA_pol_sigma_54_core-bd.
IPR007634. RNA_pol_sigma_54_DNA-bd.
IPR036388. WH-like_DNA-bd_sf.
PANTHERiPTHR32248. PTHR32248. 1 hit.
PfamiView protein in Pfam
PF00309. Sigma54_AID. 1 hit.
PF04963. Sigma54_CBD. 1 hit.
PF04552. Sigma54_DBD. 1 hit.
PIRSFiPIRSF000774. RpoN. 1 hit.
PRINTSiPR00045. SIGMA54FCT.
TIGRFAMsiTIGR02395. rpoN_sigma. 1 hit.
PROSITEiView protein in PROSITE
PS00717. SIGMA54_1. 1 hit.
PS00718. SIGMA54_2. 1 hit.
PS50044. SIGMA54_3. 1 hit.

Sequencei

Sequence statusi: Complete.

P33984-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMSPKMEFR QSQSLVMTPQ LMQAIKLLQL SNLELVAYVE AELERNPLLE
60 70 80 90 100
RASEPESPEH DPPNPQEEAP TPPDSGAPVS GDWMESDMGS SREAIETRLD
110 120 130 140 150
TDLGNVFPDD APAERIGAGS GSGSSIEWGS GGDRGEDYNP EAFLAAETTL
160 170 180 190 200
ADHLEAQLSV AEPDPARRLI GLNLIGLIDE TGYFSGDLDA VAEQLGATHD
210 220 230 240 250
QVADVLRVIQ SFEPSGVGAR SLSECLALQL RDKDRCDPAM QALLDNLELL
260 270 280 290 300
ARHDRNALKR ICGVDAEDLA DMIGEIRRLD PKPGLAYGGG VVHPLVPDVF
310 320 330 340 350
VREGSDGSWI VELNSETLPR VLVNQTYHAT VAKAARSAEE KTFLADCLQS
360 370 380 390 400
ASWLTRSLDQ RARTILKVAS EIVRQQDAFL VHGVRHLRPL NLRTVADAIG
410 420 430 440 450
MHESTVSRVT SNKYISTPRG VLEMKFFFSS SIASSGGGEA HAAEAVRHRI
460 470 480 490 500
KSLIEAESAD DVLSDDTLVQ KLKDDGIDIA RRTVAKYRES MNIPSSVQRR
510
REKQALRSDA AAAG
Length:514
Mass (Da):55,888
Last modified:February 26, 2008 - v2
Checksum:iFEE25CA7530F1481
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti60H → L in CAA49582 (PubMed:8471796).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69959 Genomic DNA. Translation: CAA49582.1.
AP009384 Genomic DNA. Translation: BAF89923.1.
PIRiS33580.
RefSeqiWP_012172445.1. NC_009937.1.

Genome annotation databases

EnsemblBacteriaiBAF89923; BAF89923; AZC_3925.
KEGGiazc:AZC_3925.

Similar proteinsi

Entry informationi

Entry nameiRP54_AZOC5
AccessioniPrimary (citable) accession number: P33984
Secondary accession number(s): A8IKL6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 26, 2008
Last modified: October 25, 2017
This is version 94 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families