P33975 (TRPE_HALVD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anthranilate synthase component 1
EC=4.1.3.27

Alternative name(s):
Anthranilate synthase component I

Gene names

Name:	trpE
Ordered Locus Names:	HVO_2454

Organism

Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) [Complete proteome] [HAMAP]

Taxonomic identifier

309800 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloferax

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.
Subunit structure	Tetramer of two components I and two components II By similarity.
Miscellaneous	Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.
Sequence similarities	Belongs to the anthranilate synthase component I family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tryptophan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	anthranilate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 524	524	Anthranilate synthase component 1		PRO_0000154127
Experimental info
	Sequence conflict	66 – 72	7	GAFSAPA → ARSPRPR in AAA73177. Ref.1
	Sequence conflict	140 – 143	4	RQTL → ADV in AAA73177. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P33975 [UniParc]. Last modified September 21, 2011. Version 2. Checksum: FB1D1CCD3A4132AC	FASTA	524	55,915
	10 20 30 40 50 60 MTAPDTDREE FVSLAGDADG PVVTHLVADL DVSVDPLAAY TTLADRSDYG FLLESAEKVS 70 80 90 100 110 120 SSNPQGAFSA PATAADSHAR FSFVGYDPEA VVTVGPDGVD VTDLGGPAAE FVGAGDGDVL 130 140 150 160 170 180 DSLRGALPDL PRVNFPETDR QTLTGGLVGF LAYEAVYDLW LDEVGRERPD TDDPDAEFVL 190 200 210 220 230 240 TTRTLSFDHR EDAVRLVCTP VVSPDDDPGE VYDGVVAEAE RVAEKLRAAD DPAPGGFERT 250 260 270 280 290 300 GEDAGSREEY EAAVRKTKEH VRDGDIYQGV ISRTRKLRGQ VDPVGLYASL REVNPSPYMF 310 320 330 340 350 360 LLRHGDRRVV GASPETLVSV RGDRVVVNPI AGTCQRGSGP VEDRRLAGEL LADAKERAEH 370 380 390 400 410 420 TMLVDLGRND VRRVSTPGSV RVEDFMSIIK YSHVQHIEST VSGTLDADAD AFDATRATFP 430 440 450 460 470 480 AGTLTGAPKV RAMEIIDDLE AEPRGVYGGG VGYYSWTGDA DVAIVIRTAT VDSGGADDAI 490 500 510 520 TVRAGAGIVA DSDPTAEYEE TEQKMGGVLD AVRRIEYGTE EASQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genes for tryptophan biosynthesis in the halophilic archaebacterium Haloferax volcanii: the trpDFEG cluster." Lam W.L., Logan S.M., Doolittle W.F. J. Bacteriol. 174:1694-1697(1992) [PubMed: 1537810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DS2 / WFD11.
[2]	"The complete genome sequence of Haloferax volcanii DS2, a model archaeon." Hartman A.L., Norais C., Badger J.H., Delmas S., Haldenby S., Madupu R., Robinson J., Khouri H., Ren Q., Lowe T.M., Maupin-Furlow J., Pohlschroder M., Daniels C., Pfeiffer F., Allers T., Eisen J.A. PLoS ONE 5:E9605-E9605(2010) [PubMed: 20333302] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M83788 Genomic DNA. Translation: AAA73177.1. CP001956 Genomic DNA. Translation: ADE02423.1.
PIR	A42301.
RefSeq	YP_003536475.1. NC_013967.1.
3D structure databases
ProteinModelPortal	P33975.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8924704.
KEGG	hvo:HVO_2454.
Organism-specific databases
CMR	Search...
Family and domain databases
InterPro	IPR005801. ADC_synthase. IPR019999. Anth_synth_I. IPR006805. Anth_synth_I_N. IPR010116. Anthranilate_synth_I_arc. IPR015890. Chorismate-bd_C. [Graphical view]
Gene3D	G3DSA:3.60.120.10. TRPE_1_chor_bd. 1 hit.
KO	K01657.
Pfam	PF04715. Anth_synt_I_N. 1 hit. PF00425. Chorismate_bind. 1 hit. [Graphical view]
PRINTS	PR00095. ANTSNTHASEI.
SUPFAM	SSF56322. TRPE_1_chor_bd. 1 hit.
TIGRFAMs	TIGR01820. TrpE-arch. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRPE_HALVD

Accession

Primary (citable) accession number: P33975
Secondary accession number(s): D4GT37

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	September 21, 2011
Last modified:	December 14, 2011
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program