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Protein

ABC transporter ATP-binding/permease protein YojI

Gene

yojI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mediates resistance to the antibacterial peptide microcin J25, when expressed from a multicopy vector. Functions as an efflux pump for microcin J25, with the help of the outer membrane channel TolC.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi356 – 3638ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATPase activity, coupled to transmembrane movement of substances Source: InterPro
  • ATP binding Source: UniProtKB-KW
  • peptide transporter activity Source: EcoCyc

GO - Biological processi

  • microcin transport Source: EcoliWiki
  • response to antibiotic Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Antibiotic resistance, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:YOJI-MONOMER.
ECOL316407:JW2199-MONOMER.

Protein family/group databases

TCDBi3.A.1.113.3. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
ABC transporter ATP-binding/permease protein YojICurated
Gene namesi
Name:yojI
Synonyms:yojJ
Ordered Locus Names:b2211, JW2199
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12070. yojI.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei16 – 3621HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei47 – 6721HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei125 – 14521HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei149 – 16921HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei240 – 26021HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei270 – 29021HelicalPROSITE-ProRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Disruption increases sensitivity of cells to microcin J25.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 547547ABC transporter ATP-binding/permease protein YojIPRO_0000093196Add
BLAST

Proteomic databases

PaxDbiP33941.
PRIDEiP33941.

Interactioni

Protein-protein interaction databases

BioGridi4261919. 151 interactions.
IntActiP33941. 5 interactions.
STRINGi511145.b2211.

Structurei

3D structure databases

ProteinModelPortaliP33941.
SMRiP33941. Positions 8-535.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 291277ABC transmembrane type-1PROSITE-ProRule annotationAdd
BLAST
Domaini323 – 547225ABC transporterPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ABC transporter superfamily.Curated
Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation
Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105IBP. Bacteria.
COG4615. LUCA.
HOGENOMiHOG000260219.
InParanoidiP33941.
KOiK06159.
OMAiIAFINLR.
OrthoDBiEOG6GN713.
PhylomeDBiP33941.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR005898. Cyc_pep_transpt.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
TIGRFAMsiTIGR01194. cyc_pep_trnsptr. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33941-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELLVLVWRQ YRWPFISVMA LSLASAALGI GLIAFINQRL IETADTSLLV
60 70 80 90 100
LPEFLGLLLL LMAVTLGSQL ALTTLGHHFV YRLRSEFIKR ILDTHVERIE
110 120 130 140 150
QLGSASLLAG LTSDVRNITI AFVRLPELVQ GIILTIGSAA YLWMLSGKML
160 170 180 190 200
LVTAIWMAIT IWGGFVLVAR VYKHMATLRE TEDKLYTDFQ TVLEGRKELT
210 220 230 240 250
LNRERAEYVF NNLYIPDAQE YRHHIIRADT FHLSAVNWSN IMMLGAIGLV
260 270 280 290 300
FWMANSLGWA DTNVAATYSL TLLFLRTPLL SAVGALPTLL TAQVAFNKLN
310 320 330 340 350
KFALAPFKAE FPRPQAFPNW QTLELRNVTF AYQDNAFSVG PINLTIKRGE
360 370 380 390 400
LLFLIGGNGS GKSTLAMLLT GLYQPQSGEI LLDGKPVSGE QPEDYRKLFS
410 420 430 440 450
AVFTDVWLFD QLLGPEGKPA NPQLVEKWLA QLKMAHKLEL SNGRIVNLKL
460 470 480 490 500
SKGQKKRVAL LLALAEERDI ILLDEWAADQ DPHFRREFYQ VLLPLMQEMG
510 520 530 540
KTIFAISHDD HYFIHADRLL EMRNGQLSEL TGEERDAASR DAVARTA
Length:547
Mass (Da):61,553
Last modified:June 1, 1994 - v2
Checksum:i11523935EDFD9EAB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00008 Genomic DNA. Translation: AAA16403.1.
U00096 Genomic DNA. Translation: AAC75271.1.
AP009048 Genomic DNA. Translation: BAA15994.1.
PIRiA64991.
RefSeqiNP_416715.1. NC_000913.3.
WP_000422182.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75271; AAC75271; b2211.
BAA15994; BAA15994; BAA15994.
GeneIDi946705.
KEGGiecj:JW2199.
eco:b2211.
PATRICi32119781. VBIEscCol129921_2300.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00008 Genomic DNA. Translation: AAA16403.1.
U00096 Genomic DNA. Translation: AAC75271.1.
AP009048 Genomic DNA. Translation: BAA15994.1.
PIRiA64991.
RefSeqiNP_416715.1. NC_000913.3.
WP_000422182.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP33941.
SMRiP33941. Positions 8-535.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261919. 151 interactions.
IntActiP33941. 5 interactions.
STRINGi511145.b2211.

Protein family/group databases

TCDBi3.A.1.113.3. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP33941.
PRIDEiP33941.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75271; AAC75271; b2211.
BAA15994; BAA15994; BAA15994.
GeneIDi946705.
KEGGiecj:JW2199.
eco:b2211.
PATRICi32119781. VBIEscCol129921_2300.

Organism-specific databases

EchoBASEiEB1997.
EcoGeneiEG12070. yojI.

Phylogenomic databases

eggNOGiENOG4105IBP. Bacteria.
COG4615. LUCA.
HOGENOMiHOG000260219.
InParanoidiP33941.
KOiK06159.
OMAiIAFINLR.
OrthoDBiEOG6GN713.
PhylomeDBiP33941.

Enzyme and pathway databases

BioCyciEcoCyc:YOJI-MONOMER.
ECOL316407:JW2199-MONOMER.

Miscellaneous databases

PROiP33941.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR005898. Cyc_pep_transpt.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
TIGRFAMsiTIGR01194. cyc_pep_trnsptr. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Automated multiplex sequencing of the E.coli genome."
    Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M.
    Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / BHB2600.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "YojI of Escherichia coli functions as a microcin J25 efflux pump."
    Delgado M.A., Vincent P.A., Farias R.N., Salomon R.A.
    J. Bacteriol. 187:3465-3470(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN MICROCIN J25 RESISTANCE, DISRUPTION PHENOTYPE.
    Strain: K12.

Entry informationi

Entry nameiYOJI_ECOLI
AccessioniPrimary (citable) accession number: P33941
Secondary accession number(s): P33942
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: June 1, 1994
Last modified: April 13, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.