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Protein

Malate:quinone oxidoreductase

Gene

mqo

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + a quinone = oxaloacetate + reduced quinone.

Cofactori

FADBy similarity

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes oxaloacetate from (S)-malate (quinone route).
Proteins known to be involved in this subpathway in this organism are:
  1. Malate:quinone oxidoreductase (mqo)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes oxaloacetate from (S)-malate (quinone route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

GO - Molecular functioni

  • flavin adenine dinucleotide binding Source: EcoCyc
  • malate dehydrogenase (menaquinone) activity Source: UniProtKB-EC
  • malate dehydrogenase (quinone) activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciEcoCyc:EG12069-MONOMER.
ECOL316407:JW2198-MONOMER.
MetaCyc:EG12069-MONOMER.
UniPathwayiUPA00223; UER01008.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate:quinone oxidoreductase (EC:1.1.5.4)
Alternative name(s):
MQO
Malate dehydrogenase [quinone]
Gene namesi
Name:mqo
Synonyms:yojH
Ordered Locus Names:b2210, JW2198
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12069. mqo.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic side of plasma membrane Source: EcoCyc
  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 548548Malate:quinone oxidoreductasePRO_0000128712Add
BLAST

Proteomic databases

PaxDbiP33940.
PRIDEiP33940.

Interactioni

Protein-protein interaction databases

IntActiP33940. 18 interactions.
STRINGi511145.b2210.

Structurei

3D structure databases

ProteinModelPortaliP33940.
SMRiP33940. Positions 28-69, 215-283.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MQO family.Curated

Phylogenomic databases

eggNOGiENOG4105DWT. Bacteria.
COG0579. LUCA.
HOGENOMiHOG000109379.
InParanoidiP33940.
KOiK00116.
OMAiEPIAATK.
PhylomeDBiP33940.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
HAMAPiMF_00212. MQO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR006231. MQO.
[Graphical view]
PfamiPF06039. Mqo. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR01320. mal_quin_oxido. 1 hit.

Sequencei

Sequence statusi: Complete.

P33940-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKVTAMLFS MAVGLNAVSM AAKAKASEEQ ETDVLLIGGG IMSATLGTYL
60 70 80 90 100
RELEPEWSMT MVERLEGVAQ ESSNGWNNAG TGHSALMELN YTPQNADGSI
110 120 130 140 150
SIEKAVAINE AFQISRQFWA HQVERGVLRT PRSFINTVPH MSFVWGEDNV
160 170 180 190 200
NFLRARYAAL QQSSLFRGMR YSEDHAQIKE WAPLVMEGRD PQQKVAATRT
210 220 230 240 250
EIGTDVNYGE ITRQLIASLQ KKSNFSLQLS SEVRALKRND DNTWTVTVAD
260 270 280 290 300
LKNGTAQNIR AKFVFIGAGG AALKLLQESG IPEAKDYAGF PVGGQFLVSE
310 320 330 340 350
NPDVVNHHLA KVYGKASVGA PPMSVPHIDT RVLDGKRVVL FGPFATFSTK
360 370 380 390 400
FLKNGSLWDL MSSTTTSNVM PMMHVGLDNF DLVKYLVSQV MLSEEDRFEA
410 420 430 440 450
LKEYYPQAKK EDWRLWQAGQ RVQIIKRDAE KGGVLRLGTE VVSDQQGTIA
460 470 480 490 500
ALLGASPGAS TAAPIMLNLL EKVFGDRVSS PQWQATLKAI VPSYGRKLNG
510 520 530 540
DVAATERELQ YTSEVLGLNY DKPQAADSTP KPQLKPQPVQ KEVADIAL
Length:548
Mass (Da):60,230
Last modified:November 1, 1997 - v2
Checksum:i3278A6D124EEE2AA
GO

Sequence cautioni

The sequence AAA16402 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00008 Genomic DNA. Translation: AAA16402.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75270.1.
AP009048 Genomic DNA. Translation: BAA15993.1.
PIRiH64990.
RefSeqiNP_416714.1. NC_000913.3.
WP_000758077.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75270; AAC75270; b2210.
BAA15993; BAA15993; BAA15993.
GeneIDi946702.
KEGGiecj:JW2198.
eco:b2210.
PATRICi32119779. VBIEscCol129921_2299.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00008 Genomic DNA. Translation: AAA16402.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75270.1.
AP009048 Genomic DNA. Translation: BAA15993.1.
PIRiH64990.
RefSeqiNP_416714.1. NC_000913.3.
WP_000758077.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP33940.
SMRiP33940. Positions 28-69, 215-283.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP33940. 18 interactions.
STRINGi511145.b2210.

Proteomic databases

PaxDbiP33940.
PRIDEiP33940.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75270; AAC75270; b2210.
BAA15993; BAA15993; BAA15993.
GeneIDi946702.
KEGGiecj:JW2198.
eco:b2210.
PATRICi32119779. VBIEscCol129921_2299.

Organism-specific databases

EchoBASEiEB1996.
EcoGeneiEG12069. mqo.

Phylogenomic databases

eggNOGiENOG4105DWT. Bacteria.
COG0579. LUCA.
HOGENOMiHOG000109379.
InParanoidiP33940.
KOiK00116.
OMAiEPIAATK.
PhylomeDBiP33940.

Enzyme and pathway databases

UniPathwayiUPA00223; UER01008.
BioCyciEcoCyc:EG12069-MONOMER.
ECOL316407:JW2198-MONOMER.
MetaCyc:EG12069-MONOMER.

Miscellaneous databases

PROiP33940.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
HAMAPiMF_00212. MQO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR006231. MQO.
[Graphical view]
PfamiPF06039. Mqo. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR01320. mal_quin_oxido. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMQO_ECOLI
AccessioniPrimary (citable) accession number: P33940
Secondary accession number(s): O08017, P76454
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.