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Protein

Ferredoxin-type protein NapH

Gene

napH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for electron transfer from ubiquinol, via NapC, to the periplasmic nitrate reductase NapAB complex.2 Publications

Cofactori

[4Fe-4S] clusterPROSITE-ProRule annotationNote: Binds 2 [4Fe-4S] cluster.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi226Iron-sulfur 1 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi229Iron-sulfur 1 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi232Iron-sulfur 1 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi236Iron-sulfur 1 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi260Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi263Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi266Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi270Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processElectron transport, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:NAPH-MONOMER
MetaCyc:NAPH-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Ferredoxin-type protein NapHCurated
Alternative name(s):
Ubiquinol--[NapC cytochrome c] reductase NapH subunitCurated
Gene namesi
Name:napH
Synonyms:yejZ
Ordered Locus Names:b2204, JW2192
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12062 napH

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 29Cytoplasmic1 PublicationAdd BLAST29
Transmembranei30 – 50HelicalSequence analysisAdd BLAST21
Topological domaini51 – 79Periplasmic1 PublicationAdd BLAST29
Transmembranei80 – 100HelicalSequence analysisAdd BLAST21
Topological domaini101 – 139Cytoplasmic1 PublicationAdd BLAST39
Transmembranei140 – 160HelicalSequence analysisAdd BLAST21
Topological domaini161 – 170Periplasmic1 Publication10
Transmembranei171 – 191HelicalSequence analysisAdd BLAST21
Topological domaini192 – 287Cytoplasmic1 PublicationAdd BLAST96

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: EcoliWiki
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion of both napG and napH genes has little effect on the overall rate of nitrate reduction during growth in the glycerol/nitrate medium. However, during growth in the glucose/nitrate medium, the double mutant shows a decreased rate of electron transfer that correlates with decreased NapAB activity.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001592831 – 287Ferredoxin-type protein NapHAdd BLAST287

Proteomic databases

PaxDbiP33934
PRIDEiP33934

Interactioni

Subunit structurei

Interacts with NapC.1 Publication

Protein-protein interaction databases

BioGridi4262223, 14 interactors
DIPiDIP-10310N
IntActiP33934, 2 interactors
STRINGi316385.ECDH10B_2361

Structurei

3D structure databases

ProteinModelPortaliP33934
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini217 – 2474Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST31
Domaini251 – 2804Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST30

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105GXZ Bacteria
COG0348 LUCA
HOGENOMiHOG000275510
InParanoidiP33934
KOiK02574
OMAiCPVGAFY
PhylomeDBiP33934

Family and domain databases

InterProiView protein in InterPro
IPR017896 4Fe4S_Fe-S-bd
IPR017900 4Fe4S_Fe_S_CS
IPR011886 NapH_MauN
PfamiView protein in Pfam
PF13237 Fer4_10, 1 hit
PF12801 Fer4_5, 2 hits
TIGRFAMsiTIGR02163 napH_, 1 hit
PROSITEiView protein in PROSITE
PS00198 4FE4S_FER_1, 1 hit
PS51379 4FE4S_FER_2, 2 hits

Sequencei

Sequence statusi: Complete.

P33934-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANRKRDAGR EALEKKGWWR SHRWLVLRRL CQFFVLGMFL SGPWFGVWIL
60 70 80 90 100
HGNYSSSLLF DTVPLTDPLM TLQSLASGHL PATVALTGAV IITVLYALAG
110 120 130 140 150
KRLFCSWVCP LNPITDLANW LRRRFDLNQS ATIPRHIRYV LLVVILVGSA
160 170 180 190 200
LTGTLIWEWI NPVSLMGRSL VMGFGSGALL ILALFLFDLL VVEHGWCGHI
210 220 230 240 250
CPVGALYGVL GSKGVITVAA TDRQKCNRCM DCFHVCPEPH VLRAPVLDEQ
260 270 280
SPVQVTSRDC MTCGRCVDVC SEDVFTITTR WSSGAKS
Length:287
Mass (Da):31,874
Last modified:February 1, 1994 - v1
Checksum:i50AF7D5E0FFBC4D4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00008 Genomic DNA Translation: AAA16396.1
U00096 Genomic DNA Translation: AAC75264.1
AP009048 Genomic DNA Translation: BAE76667.1
PIRiB64990
RefSeqiNP_416708.1, NC_000913.3
WP_000013515.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75264; AAC75264; b2204
BAE76667; BAE76667; BAE76667
GeneIDi945984
KEGGiecj:JW2192
eco:b2204
PATRICifig|1411691.4.peg.32

Similar proteinsi

Entry informationi

Entry nameiNAPH_ECOLI
AccessioniPrimary (citable) accession number: P33934
Secondary accession number(s): Q2MAN9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: March 28, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health