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Protein

Cytochrome c-type biogenesis protein CcmF

Gene

ccmF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for the biogenesis of c-type cytochromes. Possible subunit of a heme lyase.

GO - Molecular functioni

  • heme binding Source: EcoCyc
  • heme transporter activity Source: InterPro

GO - Biological processi

  • cytochrome c-heme linkage Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Cytochrome c-type biogenesis

Enzyme and pathway databases

BioCyciEcoCyc:EG12054-MONOMER.
ECOL316407:JW2184-MONOMER.

Protein family/group databases

TCDBi9.B.14.1.3. the putative heme handling protein (hhp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c-type biogenesis protein CcmF
Gene namesi
Name:ccmF
Synonyms:yejR
Ordered Locus Names:b2196, JW2184
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12054. ccmF.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88PeriplasmicSequence analysis
Transmembranei9 – 2921HelicalSequence analysisAdd
BLAST
Topological domaini30 – 4112CytoplasmicSequence analysisAdd
BLAST
Transmembranei42 – 6221HelicalSequence analysisAdd
BLAST
Topological domaini63 – 9331PeriplasmicSequence analysisAdd
BLAST
Transmembranei94 – 11421HelicalSequence analysisAdd
BLAST
Topological domaini115 – 1206CytoplasmicSequence analysis
Transmembranei121 – 14121HelicalSequence analysisAdd
BLAST
Topological domaini142 – 17534PeriplasmicSequence analysisAdd
BLAST
Transmembranei176 – 19621HelicalSequence analysisAdd
BLAST
Topological domaini197 – 21014CytoplasmicSequence analysisAdd
BLAST
Transmembranei211 – 23121HelicalSequence analysisAdd
BLAST
Topological domaini232 – 2354PeriplasmicSequence analysis
Transmembranei236 – 25621HelicalSequence analysisAdd
BLAST
Topological domaini257 – 27317CytoplasmicSequence analysisAdd
BLAST
Transmembranei274 – 29421HelicalSequence analysisAdd
BLAST
Topological domaini295 – 31117PeriplasmicSequence analysisAdd
BLAST
Transmembranei312 – 33221HelicalSequence analysisAdd
BLAST
Topological domaini333 – 35018CytoplasmicSequence analysisAdd
BLAST
Transmembranei351 – 37121HelicalSequence analysisAdd
BLAST
Topological domaini372 – 38918PeriplasmicSequence analysisAdd
BLAST
Transmembranei390 – 41021HelicalSequence analysisAdd
BLAST
Topological domaini411 – 42414CytoplasmicSequence analysisAdd
BLAST
Transmembranei425 – 44521HelicalSequence analysisAdd
BLAST
Topological domaini446 – 4472PeriplasmicSequence analysis
Transmembranei448 – 46821HelicalSequence analysisAdd
BLAST
Topological domaini469 – 48113CytoplasmicSequence analysisAdd
BLAST
Transmembranei482 – 50221HelicalSequence analysisAdd
BLAST
Topological domaini503 – 608106PeriplasmicSequence analysisAdd
BLAST
Transmembranei609 – 62921HelicalSequence analysisAdd
BLAST
Topological domaini630 – 64718CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 647647Cytochrome c-type biogenesis protein CcmFPRO_0000201583Add
BLAST

Proteomic databases

PaxDbiP33927.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ccmHP0ABM93EBI-763370,EBI-560309

Protein-protein interaction databases

BioGridi4260482. 31 interactions.
DIPiDIP-9256N.
IntActiP33927. 3 interactions.
MINTiMINT-225079.
STRINGi511145.b2196.

Structurei

3D structure databases

ProteinModelPortaliP33927.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CcmF/CycK/Ccl1/NrfE/CcsA family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DA4. Bacteria.
COG1138. LUCA.
HOGENOMiHOG000274659.
InParanoidiP33927.
KOiK02198.
OMAiQRATHRE.
OrthoDBiEOG6RRKJ4.
PhylomeDBiP33927.

Family and domain databases

InterProiIPR032523. CcmF_C.
IPR002541. Cyt_c_assembly.
IPR003567. Cyt_c_biogenesis.
IPR003568. Cyt_c_biogenesis_CcmF.
[Graphical view]
PANTHERiPTHR30009:SF0. PTHR30009:SF0. 2 hits.
PfamiPF16327. CcmF_C. 1 hit.
PF01578. Cytochrom_C_asm. 1 hit.
[Graphical view]
PRINTSiPR01410. CCBIOGENESIS.
PR01411. CCMFBIOGNSIS.
TIGRFAMsiTIGR00353. nrfE. 1 hit.

Sequencei

Sequence statusi: Complete.

P33927-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMPEIGNGLL CLALGIALLL SVYPLWGVAR GDARMMASSR LFAWLLFMSV
60 70 80 90 100
AGAFLVLVNA FVVNDFTVTY VASNSNTQLP VWYRVAATWG AHEGSLLLWV
110 120 130 140 150
LLMSGWTFAV AIFSQRIPLD IVARVLAIMG MVSVGFLLFI LFTSNPFSRT
160 170 180 190 200
LPNFPIEGRD LNPLLQDPGL IFHPPLLYMG YVGFSVAFAF AIASLLSGRL
210 220 230 240 250
DSTYARFTRP WTLAAWIFLT LGIVLGSAWA YYELGWGGWW FWDPVENASF
260 270 280 290 300
MPWLVGTALM HSLAVTEQRA SFKAWTLLLA ISAFSLCLLG TFLVRSGVLV
310 320 330 340 350
SVHAFASDPA RGMFILAFMV LVIGGSLLLF AARGHKVRSR VNNALWSRES
360 370 380 390 400
LLLANNVLLV AAMLVVLLGT LLPLVHKQLG LGSISIGEPF FNTMFTWLMV
410 420 430 440 450
PFALLLGVGP LVRWGRDRPR KIRNLLIIAF ISTLVLSLLL PWLFESKVVA
460 470 480 490 500
MTVLGLAMAC WIAVLAIAEA ALRISRGTKT TFSYWGMVAA HLGLAVTIVG
510 520 530 540 550
IAFSQNYSVE RDVRMKSGDS VDIHEYRFTF RDVKEVTGPN WRGGVATIGV
560 570 580 590 600
TRDGKPETVL YAEKRYYNTA GSMMTEAAID GGITRDLYAA LGEELENGAW
610 620 630 640
AVRLYYKPFV RWIWAGGLMM ALGGLLCLFD PRYRKRVSPQ KTAPEAV
Length:647
Mass (Da):71,389
Last modified:February 1, 1994 - v1
Checksum:i162316A1ED156DA8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00008 Genomic DNA. Translation: AAA16388.1.
U00096 Genomic DNA. Translation: AAC75256.1.
AP009048 Genomic DNA. Translation: BAE76659.1.
PIRiB64989.
RefSeqiNP_416700.1. NC_000913.3.
WP_000982447.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75256; AAC75256; b2196.
BAE76659; BAE76659; BAE76659.
GeneIDi948783.
KEGGiecj:JW2184.
eco:b2196.
PATRICi32119751. VBIEscCol129921_2285.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00008 Genomic DNA. Translation: AAA16388.1.
U00096 Genomic DNA. Translation: AAC75256.1.
AP009048 Genomic DNA. Translation: BAE76659.1.
PIRiB64989.
RefSeqiNP_416700.1. NC_000913.3.
WP_000982447.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP33927.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260482. 31 interactions.
DIPiDIP-9256N.
IntActiP33927. 3 interactions.
MINTiMINT-225079.
STRINGi511145.b2196.

Protein family/group databases

TCDBi9.B.14.1.3. the putative heme handling protein (hhp) family.

Proteomic databases

PaxDbiP33927.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75256; AAC75256; b2196.
BAE76659; BAE76659; BAE76659.
GeneIDi948783.
KEGGiecj:JW2184.
eco:b2196.
PATRICi32119751. VBIEscCol129921_2285.

Organism-specific databases

EchoBASEiEB1985.
EcoGeneiEG12054. ccmF.

Phylogenomic databases

eggNOGiENOG4105DA4. Bacteria.
COG1138. LUCA.
HOGENOMiHOG000274659.
InParanoidiP33927.
KOiK02198.
OMAiQRATHRE.
OrthoDBiEOG6RRKJ4.
PhylomeDBiP33927.

Enzyme and pathway databases

BioCyciEcoCyc:EG12054-MONOMER.
ECOL316407:JW2184-MONOMER.

Miscellaneous databases

PROiP33927.

Family and domain databases

InterProiIPR032523. CcmF_C.
IPR002541. Cyt_c_assembly.
IPR003567. Cyt_c_biogenesis.
IPR003568. Cyt_c_biogenesis_CcmF.
[Graphical view]
PANTHERiPTHR30009:SF0. PTHR30009:SF0. 2 hits.
PfamiPF16327. CcmF_C. 1 hit.
PF01578. Cytochrom_C_asm. 1 hit.
[Graphical view]
PRINTSiPR01410. CCBIOGENESIS.
PR01411. CCMFBIOGNSIS.
TIGRFAMsiTIGR00353. nrfE. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Automated multiplex sequencing of the E.coli genome."
    Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M.
    Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / BHB2600.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Escherichia coli genes required for cytochrome c maturation."
    Thoeny-Meyer L., Fischer F., Kunzler P., Ritz D., Hennecke H.
    J. Bacteriol. 177:4321-4326(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, GENE NAME.
  5. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiCCMF_ECOLI
AccessioniPrimary (citable) accession number: P33927
Secondary accession number(s): Q2MAP7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: January 20, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.