Cytochrome c-type biogenesis protein ccmF - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P33927 (CCMF_ECOLI)

Last modified November 24, 2009. Version 73. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Cytochrome c-type biogenesis protein ccmF

Gene names

Name:	ccmF
Synonyms:	yejR
Ordered Locus Names:	b2196, JW2184

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	647 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Required for the biogenesis of c-type cytochromes. Possible subunit of a heme lyase.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the ccmF/cycK/ccl1/nrfE/ccsA family.

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Ontologies

Keywords
Biological process	Cytochrome c-type biogenesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cytochrome complex assembly Inferred from electronic annotation. Source: UniProtKB-KW heme transport Inferred from electronic annotation. Source: InterPro respiratory chain complex IV assembly Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	heme transporter activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
At1g15220	Q9XI46	1	EBI-763370,EBI-763363	From a different organism.
ccmE	P69490	1	EBI-763370,EBI-1128007
ccmH	P0ABM9	1	EBI-763370,EBI-560309

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 647

647

Cytochrome c-type biogenesis protein ccmF

PRO_0000201583

Regions

Topological domain

1 – 8

Periplasmic Potential

Transmembrane

9 – 29

Potential

Topological domain

30 – 41

Cytoplasmic Potential

Transmembrane

42 – 62

Potential

Topological domain

63 – 93

Periplasmic Potential

Transmembrane

94 – 114

Potential

Topological domain

115 – 120

Cytoplasmic Potential

Transmembrane

121 – 141

Potential

Topological domain

142 – 175

Periplasmic Potential

Transmembrane

176 – 196

Potential

Topological domain

197 – 210

Cytoplasmic Potential

Transmembrane

211 – 231

Potential

Topological domain

232 – 235

Periplasmic Potential

Transmembrane

236 – 256

Potential

Topological domain

257 – 273

Cytoplasmic Potential

Transmembrane

274 – 294

Potential

Topological domain

295 – 311

Periplasmic Potential

Transmembrane

312 – 332

Potential

Topological domain

333 – 350

Cytoplasmic Potential

Transmembrane

351 – 371

Potential

Topological domain

372 – 389

Periplasmic Potential

Transmembrane

390 – 410

Potential

Topological domain

411 – 424

Cytoplasmic Potential

Transmembrane

425 – 445

Potential

Topological domain

446 – 447

Periplasmic Potential

Transmembrane

448 – 468

Potential

Topological domain

469 – 481

Cytoplasmic Potential

Transmembrane

482 – 502

Potential

Topological domain

503 – 608

106

Periplasmic Potential

Transmembrane

609 – 629

Potential

Topological domain

630 – 647

Cytoplasmic Potential

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Sequences

Sequence

Length

Mass (Da)

Tools

P33927-1 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 162316A1ED156DA8
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FASTA

647

71,389

        10         20         30         40         50         60 
MMPEIGNGLL CLALGIALLL SVYPLWGVAR GDARMMASSR LFAWLLFMSV AGAFLVLVNA 

        70         80         90        100        110        120 
FVVNDFTVTY VASNSNTQLP VWYRVAATWG AHEGSLLLWV LLMSGWTFAV AIFSQRIPLD 

       130        140        150        160        170        180 
IVARVLAIMG MVSVGFLLFI LFTSNPFSRT LPNFPIEGRD LNPLLQDPGL IFHPPLLYMG 

       190        200        210        220        230        240 
YVGFSVAFAF AIASLLSGRL DSTYARFTRP WTLAAWIFLT LGIVLGSAWA YYELGWGGWW 

       250        260        270        280        290        300 
FWDPVENASF MPWLVGTALM HSLAVTEQRA SFKAWTLLLA ISAFSLCLLG TFLVRSGVLV 

       310        320        330        340        350        360 
SVHAFASDPA RGMFILAFMV LVIGGSLLLF AARGHKVRSR VNNALWSRES LLLANNVLLV 

       370        380        390        400        410        420 
AAMLVVLLGT LLPLVHKQLG LGSISIGEPF FNTMFTWLMV PFALLLGVGP LVRWGRDRPR 

       430        440        450        460        470        480 
KIRNLLIIAF ISTLVLSLLL PWLFESKVVA MTVLGLAMAC WIAVLAIAEA ALRISRGTKT 

       490        500        510        520        530        540 
TFSYWGMVAA HLGLAVTIVG IAFSQNYSVE RDVRMKSGDS VDIHEYRFTF RDVKEVTGPN 

       550        560        570        580        590        600 
WRGGVATIGV TRDGKPETVL YAEKRYYNTA GSMMTEAAID GGITRDLYAA LGEELENGAW 

       610        620        630        640 
AVRLYYKPFV RWIWAGGLMM ALGGLLCLFD PRYRKRVSPQ KTAPEAV

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Automated multiplex sequencing of the E.coli genome." Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M. Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / BHB2600.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Escherichia coli genes required for cytochrome c maturation." Thoeny-Meyer L., Fischer F., Kunzler P., Ritz D., Hennecke H. J. Bacteriol. 177:4321-4326(1995) [PubMed: 7635817] [Abstract] Cited for: CHARACTERIZATION, GENE NAME.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

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Cross-references

Sequence databases
	U00008 Genomic DNA. Translation: AAA16388.1. U00096 Genomic DNA. Translation: AAC75256.1. AP009048 Genomic DNA. Translation: BAE76659.1.
PIR	B64989.
RefSeq	AP_002792.1. NP_416700.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:9256N.
IntAct	P33927. 3 interactions.
STRING	P33927.
Protein family/group databases
TCDB	9.B.14.1.3. putative heme handling protein (HHP) family.
Genome annotation databases
GeneID	948783.
GenomeReviews	Gene locus JW2184 in contig AP009048_GR. Gene locus b2196 in contig U00096_GR.
KEGG	ecj:JW2184. eco:b2196.
Organism-specific databases
EchoBASE	EB1985.
EcoGene	EG12054. ccmF.
CMR	Search...
Phylogenomic databases
HOGENOM	P33927.
OMA	GIVCETT
Enzyme and pathway databases
BioCyc	EcoCyc:EG12054-MON. ECOL168927:B2196-MON.
Gene expression databases
Genevestigator	P33927.
Family and domain databases
InterPro	IPR002541. Cyt_c_assembly. IPR003567. Cyt_c_biogenesis. IPR003568. Cyt_c_biogenesis_CcmF. [Graphical view]
Pfam	PF01578. Cytochrom_C_asm. 1 hit. [Graphical view]
PRINTS	PR01410. CCBIOGENESIS. PR01411. CCMFBIOGNSIS.
TIGRFAMs	TIGR00353. nrfE. 1 hit.
ProtoNet	Search...

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Entry information

Entry name

CCMF_ECOLI

Accession

Primary (citable) accession number: P33927
Secondary accession number(s): Q2MAP7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	November 24, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents