P33912 (BPA1_STRAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Non-haem bromoperoxidase BPO-A1 EC=1.11.1.- Alternative name(s): BPO1 Bromide peroxidase
Encoded on	Plasmid
Organism	Streptomyces aureofaciens
Taxonomic identifier	1894 [NCBI]
Taxonomic lineage	cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be a chlorinating enzyme involved in 7-chlorotetracycline biosynthesis.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the bacterial non-heme bromo- and chloro-peroxidases family.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis
Molecular function	Oxidoreductase Peroxidase
Technical term	3D-structure Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological_process	antibiotic biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	peroxidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.2

Chain

2 – 275

274

Non-haem bromoperoxidase BPO-A1

PRO_0000207066

Sites

Active site

95

1

By similarity

Active site

224

1

By similarity

Active site

253

1

By similarity

Experimental info

Sequence conflict

4

1

C → S AA sequence Ref.2

Sequence conflict

17

1

W → D AA sequence Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

275

Helix Strand Turn

Details...

Beta strand

3 – 5

3

Beta strand

11 – 17

7

Beta strand

19 – 26

8

Helix

33 – 36

4

Helix

37 – 45

9

Beta strand

49 – 53

5

Helix

70 – 83

14

Beta strand

88 – 94

7

Helix

97 – 108

12

Beta strand

113 – 120

8

Helix

138 – 163

26

Turn

164 – 167

4

Helix

175 – 185

11

Helix

190 – 202

13

Helix

206 – 209

4

Beta strand

216 – 221

6

Beta strand

225 – 227

3

Helix

229 – 231

3

Helix

233 – 239

7

Beta strand

244 – 248

5

Turn

253 – 257

5

Helix

261 – 273

13

Sequences

	Sequence		Length	Mass (Da)
	P33912 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: AA2F6A958C58406E	FASTA	275	30,475
	10 20 30 40 50 60 MPICTTRDGV EIFYKDWGQG RPVVFIHGWP LNGDAWQDQL KAVVDAGYRG IAHDRRGHGH 70 80 90 100 110 120 STPVWDGYDF DTFADDLNDL LTDLDLRDVT LVAHSMGGGE LARYVGRHGT GRLRSAVLLS 130 140 150 160 170 180 AIPPVMIKSD KNPDGVPDEV FDALKNGVLT ERSQFWKDTA EGFFSANRPG NKVTQGNKDA 190 200 210 220 230 240 FWYMAMAQTI EGGVRCVDAF GYTDFTEDLK KFDIPTLVVH GDDDQVVPID ATGRKSAQII 250 260 270 PNAELKVYEG SSHGIAMVPG DKEKFNRDLL EFLNK

References

[1]	"Cloning of a second non-haem bromoperoxidase gene from Streptomyces aureofaciens ATCC 10762: sequence analysis, expression in Streptomyces lividans and enzyme purification." Pelletier I., Pfeifer O., Altenbuchner J., van Pee K.-P. Microbiology 140:509-516(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 10762 / CCM 3239 / DSM 40127 / JCM 4008 / LMG 5968.
[2]	"Purification, characterization and comparison of two non-haem bromoperoxidases from Streptomyces aureofaciens ATCC 10762." Weng M., Pfeifer O., Krauss S., Lingens F., van Pee K.-H. J. Gen. Microbiol. 137:2539-2546(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-21. Strain: ATCC 10762 / CCM 3239 / DSM 40127 / JCM 4008 / LMG 5968.
[3]	"Structural investigation of the cofactor-free chloroperoxidases." Hofmann B., Tolzer S., Pelletier I., Altenbuchner J., van Pee K.-H., Hecht H.-J. J. Mol. Biol. 279:889-900(1998) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U01096 Genomic DNA. Translation: AAC43253.1.

PIR

S59929.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1A8Q	X-ray	1.75	A	2-275	[»]

ProteinModelPortal

P33912.

SMR

P33912. Positions 2-275.

ModBase

Search...

Protein family/group databases

MEROPS

S33.992.

PeroxiBase

5911. STaHalNPrx01.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
[Graphical view]

PRINTS

PR00111. ABHYDROLASE.
PR00412. EPOXHYDRLASE.

ProtoNet

Search...

Other

EvolutionaryTrace

P33912.

Entry information

Entry name

BPA1_STRAU

Accession

Primary (citable) accession number: P33912

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 66 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program