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Protein

Translational regulator CsrA

Gene

csrA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

A translational regulator that binds mRNA to regulate translation initiation and/or mRNA stability. Usually binds in the 5'-UTR at or near the Shine-Dalgarno sequence preventing ribosome-binding, thus repressing translation. Represses expression of flagellin (hag) in a post-transcriptional fashion. Specifically binds to 2 sites in the 5'-UTR of hag mRNA in a cooperative fashion; the second site overlaps the Shine-Dalgarno sequence and prevents 30S ribosomal subunit binding (PubMed:17555441). Mutation of either binding site abolishes CsrA regulation of hag expression (PubMed:17555441, PubMed:21895793). Repression is greater in the 1A96 than 168 genetic background and higher in minimal than rich medium (PubMed:17555441). Translation repression is antagonized by FliW (PubMed:21895793). Partner switching by flagellin between FliW and CsrA provides a flagellar assembly checkpoint to tightly control the timing of flagellin synthesis. Flagellin binds to assembly factor FliW, freeing CsrA to repress translation of the flagellin mRNA. When the flagellar hook is assembled flagellin is secreted, depleting intracellular flagellin, which frees FliW to interact with CsrA and inhibits CsrA binding to mRNA. This derepresses flagellin translation and provides protein for flagellar assembly. Once the flagellar filament is completed cytoplasmic flagellin levels rise and CsrA translation repression of flagellin reinitiates (PubMed:21895793, PubMed:27516547). Overexpression leads to a dramatic reduction in motility, a significant reduction in flagellin synthesis and reduced flagella assembly (PubMed:21895793).3 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRepressor, RNA-binding
Biological processTranslation regulation

Enzyme and pathway databases

BioCyciBSUB:BSU35370-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Translational regulator CsrAUniRule annotation
Gene namesi
Name:csrAUniRule annotation
Synonyms:sow1 Publication, yviG
Ordered Locus Names:BSU35370
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Increased expression of flagellin (hag), 30% decrease in motility halo (PubMed:17555441). Suppresses the motility loss and flagellar assembly defect of an fliW deletion (PubMed:21895793). Suppresses the phenotypes associated with deletion of the intracellular flagella chaperone fliS (PubMed:23144244).3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi6R → L or W in sow-2 and sow28; suppresses the motility loss and flagellar assembly defect of an fliW deletion. Binds FliW. 2 Publications1
Mutagenesisi7K → E in sow-12; suppresses the motility loss and flagellar assembly defect of an fliW deletion. Binds FliW. 2 Publications1
Mutagenesisi14I → M: Inhibits motility and flagellar filament assembly, still binds FliW. Binds 5'-UTR of hag mRNA, is not completed by FliW. 1 Publication1
Mutagenesisi25V → G in sow-20; suppresses the motility loss and flagellar assembly defect of an fliW deletion. 1 Publication1
Mutagenesisi32L → P in sow-10; suppresses the motility loss and flagellar assembly defect of an fliW deletion. 1 Publication1
Mutagenesisi33G → R in sow-11; suppresses the motility loss and flagellar assembly defect of an fliW deletion. Binds FliW. 2 Publications1
Mutagenesisi36A → T or V in sow-8 and sow-4; suppresses the motility loss and flagellar assembly defect of an fliW deletion. Binds FliW. 2 Publications1
Mutagenesisi46E → K in sow-15; suppresses the motility loss and flagellar assembly defect of an fliW deletion. Binds FliW. 2 Publications1
Mutagenesisi49L → S: Inhibits motility and flagellar filament assembly, reduced binding of FliW. 1 Publication1
Mutagenesisi55N → D: Inhibits motility and flagellar filament assembly, greatly reduced binding of FliW. Binds 5'-UTR of hag mRNA, is not completed by FliW. 1 Publication1
Mutagenesisi58A → V: Inhibits motility and flagellar filament assembly, reduced binding of FliW. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001770491 – 74Translational regulator CsrAAdd BLAST74

Proteomic databases

PaxDbiP33911
PRIDEiP33911

Expressioni

Inductioni

Part of the SigD-controlled yvyF-csrA operon and the SigA-controlled fliW-csrA operon (PubMed:17555441). Expressed from the SigA operon at low levels during log phase, with a 5-fold increase as the culture transitions to stationary phase, peaking 1 hour later.1 Publication

Interactioni

Subunit structurei

Homodimer (PubMed:17555441). The beta-strands of each monomer intercalate to form a hydrophobic core while the alpha-helices form wings that extend away from the core (By similarity). Two molecules of FliW interact with 1 homodimer (PubMed:21895793, PubMed:27516547). mRNA and FliW bind to different sites on CsrA (PubMed:27516547).By similarity1 Publication2 Publications

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100019136

Structurei

Secondary structure

174
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1 – 5Combined sources5
Beta strandi12 – 14Combined sources3
Turni15 – 17Combined sources3
Beta strandi18 – 20Combined sources3
Beta strandi30 – 38Combined sources9
Turni52 – 54Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T3ONMR-A1-74[»]
ProteinModelPortaliP33911
SMRiP33911
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP33911

Family & Domainsi

Domaini

Contacts FliW via its C-terminus (residues 49-58).1 Publication

Sequence similaritiesi

Belongs to the CsrA/RsmA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG41085Y9 Bacteria
COG1551 LUCA
HOGENOMiHOG000020767
InParanoidiP33911
KOiK03563
OMAiIHRKEVY
PhylomeDBiP33911

Family and domain databases

Gene3Di3.30.70.1680, 1 hit
HAMAPiMF_00167 CsrA, 1 hit
InterProiView protein in InterPro
IPR003751 CsrA
IPR036107 CsrA_sf
PANTHERiPTHR34984 PTHR34984, 1 hit
PfamiView protein in Pfam
PF02599 CsrA, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD009007 CsrA, 1 hit
SUPFAMiSSF117130 SSF117130, 1 hit
TIGRFAMsiTIGR00202 csrA, 1 hit

Sequencei

Sequence statusi: Complete.

P33911-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVLSRKINE AIQIGADIEV KVIAVEGDQV KLGIDAPKHI DIHRKEIYLT
60 70
IQEENNRAAA LSSDVISALS SQKK
Length:74
Mass (Da):8,136
Last modified:February 1, 1994 - v1
Checksum:i10E82DA62D3174B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26948 Genomic DNA No translation available.
U56901 Genomic DNA Translation: AAC44950.1
AL009126 Genomic DNA Translation: CAB15554.1
PIRiH69608
RefSeqiNP_391417.1, NC_000964.3
WP_003219727.1, NZ_JNCM01000033.1

Genome annotation databases

EnsemblBacteriaiCAB15554; CAB15554; BSU35370
GeneIDi936731
KEGGibsu:BSU35370
PATRICifig|224308.179.peg.3828

Similar proteinsi

Entry informationi

Entry nameiCSRA_BACSU
AccessioniPrimary (citable) accession number: P33911
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 23, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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