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Protein

Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA

Gene

MAN1A1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

Cofactori

Enzyme regulationi

Inhibited by both 1-deoxymannojirimycin and kifunensine.

Pathwayi

GO - Molecular functioni

  1. calcium ion binding Source: ProtInc
  2. mannosidase activity Source: ProtInc
  3. mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: ProtInc

GO - Biological processi

  1. cellular protein metabolic process Source: Reactome
  2. post-translational protein modification Source: Reactome
  3. protein N-linked glycosylation via asparagine Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiREACT_25396. Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (EC:3.2.1.113)
Alternative name(s):
Man(9)-alpha-mannosidase
Short name:
Man9-mannosidase
Mannosidase alpha class 1A member 1
Processing alpha-1,2-mannosidase IA
Short name:
Alpha-1,2-mannosidase IA
Gene namesi
Name:MAN1A1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:6821. MAN1A1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4141CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei42 – 6221Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini63 – 653591LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. endoplasmic reticulum Source: ProtInc
  3. endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB
  4. extracellular vesicular exosome Source: UniProtKB
  5. Golgi apparatus Source: HPA
  6. Golgi membrane Source: Reactome
  7. integral component of membrane Source: UniProtKB-KW
  8. membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA30570.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 653653Mannosyl-oligosaccharide 1,2-alpha-mannosidase IAPRO_0000210308Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi476 ↔ 508By similarity
Glycosylationi513 – 5131N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP33908.
PaxDbiP33908.
PRIDEiP33908.

PTM databases

PhosphoSiteiP33908.

Expressioni

Gene expression databases

BgeeiP33908.
CleanExiHS_MAN1A1.
GenevestigatoriP33908.

Organism-specific databases

HPAiCAB037058.
HPA053198.
HPA063561.

Interactioni

Protein-protein interaction databases

BioGridi110294. 3 interactions.
STRINGi9606.ENSP00000357453.

Structurei

3D structure databases

ProteinModelPortaliP33908.
SMRiP33908. Positions 176-641.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG300315.
GeneTreeiENSGT00390000016529.
HOGENOMiHOG000181988.
HOVERGENiHBG052389.
InParanoidiP33908.
KOiK01230.
OMAiYARTTLK.
OrthoDBiEOG7X0VH1.
PhylomeDBiP33908.
TreeFamiTF313420.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PANTHERiPTHR11742. PTHR11742. 1 hit.
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P33908-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPVGGLLPLF SSPAGGVLGG GLGGGGGRKG SGPAALRLTE KFVLLLVFSA
60 70 80 90 100
FITLCFGAIF FLPDSSKLLS GVLFHSSPAL QPAADHKPGP GARAEDAAEG
110 120 130 140 150
RARRREEGAP GDPEAALEDN LARIRENHER ALREAKETLQ KLPEEIQRDI
160 170 180 190 200
LLEKKKVAQD QLRDKAPFRG LPPVDFVPPI GVESREPADA AIREKRAKIK
210 220 230 240 250
EMMKHAWNNY KGYAWGLNEL KPISKGGHSS SLFGNIKGAT IVDALDTLFI
260 270 280 290 300
MEMKHEFEEA KSWVEENLDF NVNAEISVFE VNIRFVGGLL SAYYLSGEEI
310 320 330 340 350
FRKKAVELGV KLLPAFHTPS GIPWALLNMK SGIGRNWPWA SGGSSILAEF
360 370 380 390 400
GTLHLEFMHL SHLSGNPIFA EKVMNIRTVL NKLEKPQGLY PNYLNPSSGQ
410 420 430 440 450
WGQHHVSVGG LGDSFYEYLL KAWLMSDKTD LEAKKMYFDA VQAIETHLIR
460 470 480 490 500
KSSSGLTYIA EWKGGLLEHK MGHLTCFAGG MFALGADAAP EGMAQHYLEL
510 520 530 540 550
GAEIARTCHE SYNRTFMKLG PEAFRFDGGV EAIATRQNEK YYILRPEVME
560 570 580 590 600
TYMYMWRLTH DPKYRKWAWE AVEALENHCR VNGGYSGLRD VYLLHESYDD
610 620 630 640 650
VQQSFFLAET LKYLYLIFSD DDLLPLEHWI FNSEAHLLPI LPKDKKEVEI

REE
Length:653
Mass (Da):72,969
Last modified:April 25, 2005 - v3
Checksum:i9B164926636D8554
GO
Isoform 2 (identifier: P33908-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     201-201: E → EEIEARKGQANCPGSSSSCEVEIQ
     331-344: SGIGRNWPWASGGS → RWMCNIPRAMEATI
     345-653: Missing.

Note: No experimental confirmation available.

Show »
Length:367
Mass (Da):40,037
Checksum:i3A5E1D30321CAFF8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti29 – 4719KGSGP…VLLLV → MNSNFITFDLKMSLLPSNL in CAA52831 (PubMed:8223597).CuratedAdd
BLAST
Sequence conflicti464 – 4641G → R in CAA52831 (PubMed:8223597).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti651 – 6511R → G.
Corresponds to variant rs35544784 [ dbSNP | Ensembl ].
VAR_034102

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei201 – 2011E → EEIEARKGQANCPGSSSSCE VEIQ in isoform 2. 1 PublicationVSP_056372
Alternative sequencei331 – 34414SGIGR…ASGGS → RWMCNIPRAMEATI in isoform 2. 1 PublicationVSP_056373Add
BLAST
Alternative sequencei345 – 653309Missing in isoform 2. 1 PublicationVSP_056374Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL022722, AL078600 Genomic DNA. Translation: CAI20315.1.
AL078600 Genomic DNA. Translation: CAB75695.2.
AL138886 Genomic DNA. No translation available.
CH471051 Genomic DNA. Translation: EAW48185.1.
BC065827 mRNA. Translation: AAH65827.1.
X74837 mRNA. Translation: CAA52831.1.
CCDSiCCDS5122.1. [P33908-1]
PIRiS38965.
RefSeqiNP_005898.2. NM_005907.3. [P33908-1]
UniGeneiHs.102788.
Hs.742378.

Genome annotation databases

EnsembliENST00000368468; ENSP00000357453; ENSG00000111885. [P33908-1]
GeneIDi4121.
KEGGihsa:4121.
UCSCiuc003pym.2. human. [P33908-1]
uc010kei.3. human.

Polymorphism databases

DMDMi62906886.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL022722, AL078600 Genomic DNA. Translation: CAI20315.1.
AL078600 Genomic DNA. Translation: CAB75695.2.
AL138886 Genomic DNA. No translation available.
CH471051 Genomic DNA. Translation: EAW48185.1.
BC065827 mRNA. Translation: AAH65827.1.
X74837 mRNA. Translation: CAA52831.1.
CCDSiCCDS5122.1. [P33908-1]
PIRiS38965.
RefSeqiNP_005898.2. NM_005907.3. [P33908-1]
UniGeneiHs.102788.
Hs.742378.

3D structure databases

ProteinModelPortaliP33908.
SMRiP33908. Positions 176-641.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110294. 3 interactions.
STRINGi9606.ENSP00000357453.

Chemistry

ChEMBLiCHEMBL5915.

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

PTM databases

PhosphoSiteiP33908.

Polymorphism databases

DMDMi62906886.

Proteomic databases

MaxQBiP33908.
PaxDbiP33908.
PRIDEiP33908.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000368468; ENSP00000357453; ENSG00000111885. [P33908-1]
GeneIDi4121.
KEGGihsa:4121.
UCSCiuc003pym.2. human. [P33908-1]
uc010kei.3. human.

Organism-specific databases

CTDi4121.
GeneCardsiGC06M119540.
HGNCiHGNC:6821. MAN1A1.
HPAiCAB037058.
HPA053198.
HPA063561.
MIMi604344. gene.
neXtProtiNX_P33908.
PharmGKBiPA30570.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG300315.
GeneTreeiENSGT00390000016529.
HOGENOMiHOG000181988.
HOVERGENiHBG052389.
InParanoidiP33908.
KOiK01230.
OMAiYARTTLK.
OrthoDBiEOG7X0VH1.
PhylomeDBiP33908.
TreeFamiTF313420.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_25396. Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2.

Miscellaneous databases

ChiTaRSiMAN1A1. human.
GeneWikiiMAN1A1.
GenomeRNAii4121.
NextBioi16180.
PROiP33908.
SOURCEiSearch...

Gene expression databases

BgeeiP33908.
CleanExiHS_MAN1A1.
GenevestigatoriP33908.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PANTHERiPTHR11742. PTHR11742. 1 hit.
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Testis.
  4. "Molecular cloning and primary structure of Man9-mannosidase from human kidney."
    Bause E., Bieberich E., Rolfs A., Voelker C., Schmidt B.
    Eur. J. Biochem. 217:535-540(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 29-653 (ISOFORM 1).
    Tissue: Kidney.
  5. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMA1A1_HUMAN
AccessioniPrimary (citable) accession number: P33908
Secondary accession number(s): E7EU32
, Q6P052, Q9NU44, Q9UJI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 1994
Last sequence update: April 25, 2005
Last modified: March 3, 2015
This is version 140 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  7. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.