Reviewed, UniProtKB/Swiss-Prot P33901 (NUOF_SALTY)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit F
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit F
    NDH-1 subunit F

Gene names

Name:	nuoF
Ordered Locus Names:	STM2324

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Cofactor	Binds 1 FMN Potential. Binds 1 4Fe-4S cluster Potential.
Subunit structure	Composed of 13 different subunits. Subunits nuoCD, E, F, and G constitute the peripheral sector of the complex.
Sequence similarities	Belongs to the complex I 51 kDa subunit family.
Sequence caution	The sequence AAA16062.1 differs from that shown. Reason: Frameshift at position 401.

Ontologies

Keywords
Ligand	4Fe-4S FMN Flavoprotein Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FMN binding Inferred from electronic annotation. Source: InterPro NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	NADH-quinone oxidoreductase subunit F		PRO_0000118569
Regions
	Nucleotide binding	61 – 70	10	NAD By similarity
	Nucleotide binding	174 – 221	48	FMN By similarity
Sites
	Metal binding	351	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	354	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	357	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	398	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	P33901-1 [UniParc]. Last modified January 23, 2002. Version 2. Checksum: E020825E359F382C	FASTA	445	49,245
	10 20 30 40 50 60 MKNVIRTPET HPLTWRLRDD KQPVWLDEYR SKNGYEGARK ALTGLSPDEI VSQVKDAGLK 70 80 90 100 110 120 GRGGAGFSTG LKWSLMPKDE SMNIRYLLCN ADEMEPGTYK DRLLMEQLPH LLVEGMLISA 130 140 150 160 170 180 FALKAYRGYI FLRGEYIEAA VHLRRAIAEA TEAGLLGKNI MGTGFDFELF VHTGAGRYIC 190 200 210 220 230 240 GEETALINSL EGRRANPRSK PPFPATSGVW GKPTCVNNVE TLCNVPAILA NGVEWYQNIS 250 260 270 280 290 300 KSKDAGTKLM GFSGRVKNPG LWELPFGTTA REILEDYAGG MRDGLKFKAW QPGGAGTDFL 310 320 330 340 350 360 TEAHLDLPME FESIGKAGSR LGTALAMAVD HEIGMVSLVR NLEEFFARES CGWCTPCRDG 370 380 390 400 410 420 LPWSVKILRA LERGEGQPGD IETLEQLCRF LGPGKTFCAH APGAVEPLQS AIKYFREEFE 430 440 AGIKQPFSNT HLINGIQPNL LKERW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mutants defective in the energy-conserving NADH dehydrogenase of Salmonella typhimurium identified by a decrease in energy-dependent proteolysis after carbon starvation." Archer C.D., Wang X., Elliott T. Proc. Natl. Acad. Sci. U.S.A. 90:9877-9881(1993) [PubMed: 8234329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L22504 Unassigned DNA. Translation: AAA16062.1. Frameshift. AE008804 Genomic DNA. Translation: AAL21225.1.
RefSeq	NP_461266.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1253846.
GenomeReviews	Gene locus STM2324 in contig AE006468_GR.
KEGG	stm:STM2324.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P33901.
OMA	P33901. LPHLLIE.
Enzyme and pathway databases
BioCyc	STYP99287:STM2324-MON.
BRENDA	1.6.99.5. 2.
Family and domain databases
InterPro	IPR001949. NADH-UbQ_OxRdtase_51KDa_CS. IPR019575. NADH-UbQ_OxRdtase_Fsu_4Fe4S-bd. IPR011537. NADH-UbQ_OxRdtase_suF. IPR011538. NADH_UbQ_OxRdtase_51KDa_su. IPR019554. Soluble_ligand_bd. [Graphical view]
Pfam	PF01512. Complex1_51K. 1 hit. PF10589. NADH_4Fe-4S. 1 hit. PF10531. SLBB. 1 hit. [Graphical view]
TIGRFAMs	TIGR01959. nuoF_fam. 1 hit.
PROSITE	PS00644. COMPLEX1_51K_1. 1 hit. PS00645. COMPLEX1_51K_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOF_SALTY

Accession

Primary (citable) accession number: P33901

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	January 23, 2002
Last modified:	June 16, 2009
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)