Reviewed, UniProtKB/Swiss-Prot P33898 (G3P3_ECOLI)

Names and origin

Protein names

Recommended name:
    Putative glyceraldehyde-3-phosphate dehydrogenase C
      Short name=GAPDH-C
    EC=1.2.1.12

Gene names

Name:	gapC
Ordered Locus Names:	b4493, JW1413/JW5906
ORF Names:	b1416/b1417

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Uncertain.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
Caution	Could be the product of a pseudogene; in K12 this gene is disrupted by a stop codon and a frameshift. It seems to be intact in a number of wild-type strains. Ref.8 and Ref.9 sequences were originally thought to originate from rat.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Putative glyceraldehyde-3-phosphate dehydrogenase C		PRO_0000145649
Regions
	Nucleotide binding	11 – 12	2	NAD By similarity
	Region	149 – 151	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	209 – 210	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	150	1	Nucleophile By similarity
	Binding site	34	1	NAD By similarity
	Binding site	78	1	NAD; via carbonyl oxygen By similarity
	Binding site	180	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	232	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	315	1	NAD By similarity
	Site	177	1	Activates thiol group during catalysis By similarity
Experimental info
	Sequence conflict	39	1	K → Y Ref.8
	Sequence conflict	39	1	K → Y Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P33898-1 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: E88223297376B0A0	FASTA	333	35,650
	10 20 30 40 50 60 MSKVGINGFG RIGRLVLGRL LEVKSNIDVV AINDLTSPKI LAYLLKHDSN YGPFPWSVDF 70 80 90 100 110 120 TEDSLIVDGK SIAVYAEKEA KNIPWKAKGA EIIVECTGFY TSAEKSQAHL DAGAKKVLIS 130 140 150 160 170 180 APAGEMKTIV YNVNDDTLDG NDTIVSVASC TTNCLAPMAK ALHDSFGIEV GTMTTIHAYT 190 200 210 220 230 240 GTQSLVDGPR GKDLRASRAA AENIIPHTTG AAKAIGLVIP ELSGKLKGHA QRVPVKTGSV 250 260 270 280 290 300 TELVSILGKK VTAEEVNNAL KQATTNNESF GYTDEEIVSS DIIGSHFGSV FDATQTEITA 310 320 330 VGDLQLVKTV AWYDNEYGFV TQLIRTLEKF AKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A second Escherichia coli gene with similarity to gapA." Hidalgo E., Limon A., Aguilar J. Microbiologia 12:99-106(1996) [PubMed: 9019141] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Escherichia coli genes expressed preferentially in an aquatic environment." Espinosa-Urgel M., Kolter R. Mol. Microbiol. 28:325-332(1998) [PubMed: 9622357] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Clinical isolate and River isolate.
[3]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Molecular cloning and DNA sequencing of the Escherichia coli K-12 ald gene encoding aldehyde dehydrogenase." Hidalgo E., Chen Y.-M., Lin E.C.C., Aguilar J. J. Bacteriol. 173:6118-6123(1991) [PubMed: 1917845] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 306-332. Strain: K12.
[7]	"Nucleotide sequence of the cybB gene encoding cytochrome b561 in Escherichia coli K12." Nakamura H., Murakami H., Yamato I., Anraku Y. Mol. Gen. Genet. 212:1-5(1988) [PubMed: 2836696] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-13. Strain: K12.
[8]	Krawetz S.A. Submitted (OCT-1990) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-165.
[9]	"A vector-primer-cloner-sequencer plasmid for the construction of cDNA libraries: evidence for a rat glyceraldehyde-3-phosphate dehydrogenase-like mRNA and a ferritin mRNA within testis." Krawetz S.A., Connor W., Cannon P.D., Dixon G.H. DNA 5:427-435(1986) [PubMed: 3780374] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-52.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L09067 Genomic DNA. Translation: AAA23856.1. Sequence problems. U00096 Genomic DNA. No translation available. AP009048 Genomic DNA. No translation available. M64541 Genomic DNA. No translation available. X07569 Genomic DNA. No translation available. X54798 mRNA. Translation: CAA38569.1. M14166 mRNA. Translation: AAA41178.1.
PIR	S14477.
3D structure databases
HSSP	HSSP built from PDB template 1HDG based on UniProtKB P17721.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:9737N.
Genome annotation databases
GenomeReviews	Gene locus b4493 in contig AP009048_GR.
Organism-specific databases
EchoBASE	EB2027.
EcoGene	EG12103. gapC.
CMR	Search...
Phylogenomic databases
HOGENOM	P33898.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12103-MON. EcoCyc:G8205-MON.
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DH. IPR006424. Glyceraldehyde-3-P_DH_1. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P3_ECOLI

Accession

Primary (citable) accession number: P33898
Secondary accession number(s): P76094 , P78062, P78291, Q03850, Q63208

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	April 27, 2001
Last modified:	June 16, 2009
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)