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P33895

- NUF2_YEAST

UniProt

P33895 - NUF2_YEAST

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Protein

Kinetochore protein NUF2

Gene

NUF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.2 Publications

GO - Molecular functioni

  1. structural constituent of cytoskeleton Source: SGD

GO - Biological processi

  1. chromosome segregation Source: SGD
  2. microtubule nucleation Source: SGD
  3. mitotic nuclear division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-33474-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore protein NUF2
Gene namesi
Name:NUF2
Ordered Locus Names:YOL069W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOL069w.
SGDiS000005430. NUF2.

Subcellular locationi

Nucleus. Chromosomecentromerekinetochore
Note: Associated with kinetochores.

GO - Cellular componenti

  1. condensed nuclear chromosome, centromeric region Source: SGD
  2. condensed nuclear chromosome kinetochore Source: SGD
  3. Ndc80 complex Source: SGD
  4. spindle microtubule Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi338 – 3381T → P: Temperature-sensitive. 1 Publication
Mutagenesisi340 – 3401I → K: Temperature-sensitive. 1 Publication
Mutagenesisi383 – 3831Y → C: Temperature-sensitive. 1 Publication
Mutagenesisi410 – 4101L → S: Temperature-sensitive. 1 Publication
Mutagenesisi441 – 4411K → I: Temperature-sensitive. 1 Publication
Mutagenesisi446 – 4461M → L: Temperature-sensitive. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 451451Kinetochore protein NUF2PRO_0000057995Add
BLAST

Proteomic databases

MaxQBiP33895.
PaxDbiP33895.

Expressioni

Gene expression databases

GenevestigatoriP33895.

Interactioni

Subunit structurei

Component of the NDC80 complex, which consists of TID3/NDC80, NUF2, SPC24 and SPC25. The NDC80 complex is formed by two subcomplexes, TID3/NDC80-NUF2 and SPC24-SPC25, which are joined end-to-end through their coiled-coil domains. It has a rod-like structure with a length of 570 Angstroms and globular domains at either end. The TID3/NDC80-NUF2 globular domains are probably directed to microtubules, the SPC24-SPC25 globular domains to the centromere.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CNN1P436182EBI-12377,EBI-23036
SPC24Q044778EBI-12377,EBI-27228
SPC25P400146EBI-12377,EBI-22458
TID3P4046016EBI-12377,EBI-25247

Protein-protein interaction databases

BioGridi34332. 123 interactions.
DIPiDIP-1481N.
IntActiP33895. 33 interactions.
MINTiMINT-404315.
STRINGi4932.YOL069W.

Structurei

3D structure databases

ProteinModelPortaliP33895.
SMRiP33895. Positions 7-157.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili153 – 291139Sequence AnalysisAdd
BLAST
Coiled coili341 – 37131Sequence AnalysisAdd
BLAST
Coiled coili398 – 43235Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the NUF2 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG274471.
HOGENOMiHOG000113886.
InParanoidiP33895.
KOiK11548.
OMAiFREERMF.
OrthoDBiEOG7M98RW.

Family and domain databases

InterProiIPR005549. Kinetochore_Nuf2.
[Graphical view]
PfamiPF03800. Nuf2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33895-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSRNQDVFPI LDLQELVICL QSCDFALATQ ENISRPTSDY MVTLYKQIIE
60 70 80 90 100
NFMGISVESL LNSSNQETGD GHLQEENENI YLDTLNVLVL NKICFKFFEN
110 120 130 140 150
IGVQDFNMTD LYKPEAQRTQ RLLSAVVNYA RFREERMFDC NSFILQMESL
160 170 180 190 200
LGQLRSKFDD YNLIQQQLKQ YEDVDGDNIP DEQELQKLEE QNKELEIQLK
210 220 230 240 250
KLTKIQETLS IDYNDYKISK QSIFKDLEAL SFQIVELESN RDKLIKISNT
260 270 280 290 300
DMEELSEGIK ELNDLLIQRK KTLDDLTAQQ KNLQDTVTTF ETIISELYDV
310 320 330 340 350
LRIISSEVQE SNRTETELVG LKQNLINNKL KLMNVLETGI MYKLEILQEQ
360 370 380 390 400
LDLQLKNLEK LSQDTKEESR LNDTKLMDLQ IKYENEIKPK IDKTDIFIQE
410 420 430 440 450
ELISGKINKL NDEIKQLQKD FEVEVKEIEI EYSLLSGHIN KYMNEMLEYM

Q
Length:451
Mass (Da):52,973
Last modified:February 1, 1994 - v1
Checksum:iD58796E1B5D1E772
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti240 – 2401N → Y in AAT92974. (PubMed:17322287)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X72225 Genomic DNA. Translation: CAA51028.1.
Z74811 Genomic DNA. Translation: CAA99079.1.
AY692955 Genomic DNA. Translation: AAT92974.1.
BK006948 Genomic DNA. Translation: DAA10714.1.
PIRiA53910.
RefSeqiNP_014572.1. NM_001183324.1.

Genome annotation databases

EnsemblFungiiYOL069W; YOL069W; YOL069W.
GeneIDi854085.
KEGGisce:YOL069W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X72225 Genomic DNA. Translation: CAA51028.1 .
Z74811 Genomic DNA. Translation: CAA99079.1 .
AY692955 Genomic DNA. Translation: AAT92974.1 .
BK006948 Genomic DNA. Translation: DAA10714.1 .
PIRi A53910.
RefSeqi NP_014572.1. NM_001183324.1.

3D structure databases

ProteinModelPortali P33895.
SMRi P33895. Positions 7-157.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34332. 123 interactions.
DIPi DIP-1481N.
IntActi P33895. 33 interactions.
MINTi MINT-404315.
STRINGi 4932.YOL069W.

Proteomic databases

MaxQBi P33895.
PaxDbi P33895.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOL069W ; YOL069W ; YOL069W .
GeneIDi 854085.
KEGGi sce:YOL069W.

Organism-specific databases

CYGDi YOL069w.
SGDi S000005430. NUF2.

Phylogenomic databases

eggNOGi NOG274471.
HOGENOMi HOG000113886.
InParanoidi P33895.
KOi K11548.
OMAi FREERMF.
OrthoDBi EOG7M98RW.

Enzyme and pathway databases

BioCyci YEAST:G3O-33474-MONOMER.

Miscellaneous databases

NextBioi 975728.
PROi P33895.

Gene expression databases

Genevestigatori P33895.

Family and domain databases

InterProi IPR005549. Kinetochore_Nuf2.
[Graphical view ]
Pfami PF03800. Nuf2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nuf2, a spindle pole body-associated protein required for nuclear division in yeast."
    Osborne M.A., Schlenstedt G., Jinks T., Silver P.A.
    J. Cell Biol. 125:853-866(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF THR-338; ILE-340; TYR-383; LEU-410; LYS-441 AND MET-446.
    Strain: W303A.
  2. "Sequence analysis of a 33.2 kb segment from the left arm of yeast chromosome XV reveals eight known genes and ten new open reading frames including homologues of ABC transporters, inositol phosphatases and human expressed sequence tags."
    Tzermia M., Katsoulou C., Alexandraki D.
    Yeast 13:583-589(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control."
    Janke C., Ortiz J., Lechner J., Shevchenko A., Shevchenko A., Magiera M.M., Schramm C., Schiebel E.
    EMBO J. 20:777-791(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SPC24 AND SPC25.
  7. "The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation."
    Wigge P.A., Kilmartin J.V.
    J. Cell Biol. 152:349-360(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE NDC80 COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION IN THE NDC80 COMPLEX.
  8. "The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity."
    McCleland M.L., Gardner R.D., Kallio M.J., Daum J.R., Gorbsky G.J., Burke D.J., Stukenberg P.T.
    Genes Dev. 17:101-114(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE NDC80 COMPLEX.
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "The fission yeast kinetochore component Spc7 associates with the EB1 family member Mal3 and is required for kinetochore-spindle association."
    Kerres A., Vietmeier-Decker C., Ortiz J., Karig I., Beuter C., Hegemann J., Lechner J., Fleig U.
    Mol. Biol. Cell 15:5255-5267(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE NDC80 COMPLEX.
  11. "Molecular organization of the Ndc80 complex, an essential kinetochore component."
    Wei R.R., Sorger P.K., Harrison S.C.
    Proc. Natl. Acad. Sci. U.S.A. 102:5363-5367(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF THE NDC80 COMPLEX.

Entry informationi

Entry nameiNUF2_YEAST
AccessioniPrimary (citable) accession number: P33895
Secondary accession number(s): D6W1Z8, Q6B1X5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 29, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1550 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3