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P33895

- NUF2_YEAST

UniProt

P33895 - NUF2_YEAST

Protein

Kinetochore protein NUF2

Gene

NUF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Acts as component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.2 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. structural constituent of cytoskeleton Source: SGD

    GO - Biological processi

    1. chromosome segregation Source: SGD
    2. microtubule nucleation Source: SGD
    3. mitotic nuclear division Source: UniProtKB-KW

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33474-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kinetochore protein NUF2
    Gene namesi
    Name:NUF2
    Ordered Locus Names:YOL069W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOL069w.
    SGDiS000005430. NUF2.

    Subcellular locationi

    Nucleus. Chromosomecentromerekinetochore
    Note: Associated with kinetochores.

    GO - Cellular componenti

    1. condensed nuclear chromosome, centromeric region Source: SGD
    2. condensed nuclear chromosome kinetochore Source: SGD
    3. Ndc80 complex Source: SGD
    4. spindle microtubule Source: SGD

    Keywords - Cellular componenti

    Centromere, Chromosome, Kinetochore, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi338 – 3381T → P: Temperature-sensitive. 1 Publication
    Mutagenesisi340 – 3401I → K: Temperature-sensitive. 1 Publication
    Mutagenesisi383 – 3831Y → C: Temperature-sensitive. 1 Publication
    Mutagenesisi410 – 4101L → S: Temperature-sensitive. 1 Publication
    Mutagenesisi441 – 4411K → I: Temperature-sensitive. 1 Publication
    Mutagenesisi446 – 4461M → L: Temperature-sensitive. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 451451Kinetochore protein NUF2PRO_0000057995Add
    BLAST

    Proteomic databases

    MaxQBiP33895.
    PaxDbiP33895.

    Expressioni

    Gene expression databases

    GenevestigatoriP33895.

    Interactioni

    Subunit structurei

    Component of the NDC80 complex, which consists of TID3/NDC80, NUF2, SPC24 and SPC25. The NDC80 complex is formed by two subcomplexes, TID3/NDC80-NUF2 and SPC24-SPC25, which are joined end-to-end through their coiled-coil domains. It has a rod-like structure with a length of 570 Angstroms and globular domains at either end. The TID3/NDC80-NUF2 globular domains are probably directed to microtubules, the SPC24-SPC25 globular domains to the centromere.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CNN1P436182EBI-12377,EBI-23036
    SPC24Q044778EBI-12377,EBI-27228
    SPC25P400146EBI-12377,EBI-22458
    TID3P4046016EBI-12377,EBI-25247

    Protein-protein interaction databases

    BioGridi34332. 123 interactions.
    DIPiDIP-1481N.
    IntActiP33895. 33 interactions.
    MINTiMINT-404315.
    STRINGi4932.YOL069W.

    Structurei

    3D structure databases

    ProteinModelPortaliP33895.
    SMRiP33895. Positions 7-157.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili153 – 291139Sequence AnalysisAdd
    BLAST
    Coiled coili341 – 37131Sequence AnalysisAdd
    BLAST
    Coiled coili398 – 43235Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the NUF2 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG274471.
    HOGENOMiHOG000113886.
    KOiK11548.
    OMAiFREERMF.
    OrthoDBiEOG7M98RW.

    Family and domain databases

    InterProiIPR005549. Kinetochore_Nuf2.
    [Graphical view]
    PfamiPF03800. Nuf2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P33895-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSRNQDVFPI LDLQELVICL QSCDFALATQ ENISRPTSDY MVTLYKQIIE    50
    NFMGISVESL LNSSNQETGD GHLQEENENI YLDTLNVLVL NKICFKFFEN 100
    IGVQDFNMTD LYKPEAQRTQ RLLSAVVNYA RFREERMFDC NSFILQMESL 150
    LGQLRSKFDD YNLIQQQLKQ YEDVDGDNIP DEQELQKLEE QNKELEIQLK 200
    KLTKIQETLS IDYNDYKISK QSIFKDLEAL SFQIVELESN RDKLIKISNT 250
    DMEELSEGIK ELNDLLIQRK KTLDDLTAQQ KNLQDTVTTF ETIISELYDV 300
    LRIISSEVQE SNRTETELVG LKQNLINNKL KLMNVLETGI MYKLEILQEQ 350
    LDLQLKNLEK LSQDTKEESR LNDTKLMDLQ IKYENEIKPK IDKTDIFIQE 400
    ELISGKINKL NDEIKQLQKD FEVEVKEIEI EYSLLSGHIN KYMNEMLEYM 450
    Q 451
    Length:451
    Mass (Da):52,973
    Last modified:February 1, 1994 - v1
    Checksum:iD58796E1B5D1E772
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti240 – 2401N → Y in AAT92974. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X72225 Genomic DNA. Translation: CAA51028.1.
    Z74811 Genomic DNA. Translation: CAA99079.1.
    AY692955 Genomic DNA. Translation: AAT92974.1.
    BK006948 Genomic DNA. Translation: DAA10714.1.
    PIRiA53910.
    RefSeqiNP_014572.1. NM_001183324.1.

    Genome annotation databases

    EnsemblFungiiYOL069W; YOL069W; YOL069W.
    GeneIDi854085.
    KEGGisce:YOL069W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X72225 Genomic DNA. Translation: CAA51028.1 .
    Z74811 Genomic DNA. Translation: CAA99079.1 .
    AY692955 Genomic DNA. Translation: AAT92974.1 .
    BK006948 Genomic DNA. Translation: DAA10714.1 .
    PIRi A53910.
    RefSeqi NP_014572.1. NM_001183324.1.

    3D structure databases

    ProteinModelPortali P33895.
    SMRi P33895. Positions 7-157.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34332. 123 interactions.
    DIPi DIP-1481N.
    IntActi P33895. 33 interactions.
    MINTi MINT-404315.
    STRINGi 4932.YOL069W.

    Proteomic databases

    MaxQBi P33895.
    PaxDbi P33895.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOL069W ; YOL069W ; YOL069W .
    GeneIDi 854085.
    KEGGi sce:YOL069W.

    Organism-specific databases

    CYGDi YOL069w.
    SGDi S000005430. NUF2.

    Phylogenomic databases

    eggNOGi NOG274471.
    HOGENOMi HOG000113886.
    KOi K11548.
    OMAi FREERMF.
    OrthoDBi EOG7M98RW.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33474-MONOMER.

    Miscellaneous databases

    NextBioi 975728.
    PROi P33895.

    Gene expression databases

    Genevestigatori P33895.

    Family and domain databases

    InterProi IPR005549. Kinetochore_Nuf2.
    [Graphical view ]
    Pfami PF03800. Nuf2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nuf2, a spindle pole body-associated protein required for nuclear division in yeast."
      Osborne M.A., Schlenstedt G., Jinks T., Silver P.A.
      J. Cell Biol. 125:853-866(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF THR-338; ILE-340; TYR-383; LEU-410; LYS-441 AND MET-446.
      Strain: W303A.
    2. "Sequence analysis of a 33.2 kb segment from the left arm of yeast chromosome XV reveals eight known genes and ten new open reading frames including homologues of ABC transporters, inositol phosphatases and human expressed sequence tags."
      Tzermia M., Katsoulou C., Alexandraki D.
      Yeast 13:583-589(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control."
      Janke C., Ortiz J., Lechner J., Shevchenko A., Shevchenko A., Magiera M.M., Schramm C., Schiebel E.
      EMBO J. 20:777-791(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SPC24 AND SPC25.
    7. "The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation."
      Wigge P.A., Kilmartin J.V.
      J. Cell Biol. 152:349-360(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE NDC80 COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION IN THE NDC80 COMPLEX.
    8. "The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity."
      McCleland M.L., Gardner R.D., Kallio M.J., Daum J.R., Gorbsky G.J., Burke D.J., Stukenberg P.T.
      Genes Dev. 17:101-114(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE NDC80 COMPLEX.
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. "The fission yeast kinetochore component Spc7 associates with the EB1 family member Mal3 and is required for kinetochore-spindle association."
      Kerres A., Vietmeier-Decker C., Ortiz J., Karig I., Beuter C., Hegemann J., Lechner J., Fleig U.
      Mol. Biol. Cell 15:5255-5267(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE NDC80 COMPLEX.
    11. "Molecular organization of the Ndc80 complex, an essential kinetochore component."
      Wei R.R., Sorger P.K., Harrison S.C.
      Proc. Natl. Acad. Sci. U.S.A. 102:5363-5367(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF THE NDC80 COMPLEX.

    Entry informationi

    Entry nameiNUF2_YEAST
    AccessioniPrimary (citable) accession number: P33895
    Secondary accession number(s): D6W1Z8, Q6B1X5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1550 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3