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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial

Gene

PET112

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).UniRule annotation1 Publication

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Source: SGD

GO - Biological processi

  • glutaminyl-tRNAGln biosynthesis via transamidation Source: SGD
  • mitochondrial translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28971-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrialUniRule annotation (EC:6.3.5.-UniRule annotation)
Short name:
Glu-AdT subunit BUniRule annotation
Alternative name(s):
Cytochrome c oxidase assembly factor PET112
Gene namesi
Name:PET112UniRule annotation
Ordered Locus Names:YBL080C
ORF Names:YBL0724
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBL080C.
SGDiS000000176. PET112.

Subcellular locationi

GO - Cellular componenti

  • glutamyl-tRNA(Gln) amidotransferase complex Source: SGD
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 541Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrialPRO_0000010712
Transit peptidei1 – ?MitochondrionUniRule annotation

Proteomic databases

MaxQBiP33893.

Interactioni

Subunit structurei

Subunit of the heterotrimeric GatFAB amidotransferase (AdT) complex, composed of A (HER2), B (PET112) and F (YGR102C) subunits.

Protein-protein interaction databases

BioGridi32623. 4 interactions.
DIPiDIP-5011N.
IntActiP33893. 1 interaction.
MINTiMINT-508745.

Structurei

Secondary structure

1
541
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi29 – 3810Combined sources
Beta strandi45 – 473Combined sources
Turni55 – 573Combined sources
Turni66 – 705Combined sources
Helixi80 – 9314Combined sources
Beta strandi99 – 1013Combined sources
Beta strandi104 – 1074Combined sources
Beta strandi116 – 1205Combined sources
Beta strandi122 – 1243Combined sources
Beta strandi126 – 1283Combined sources
Beta strandi131 – 1355Combined sources
Helixi136 – 1394Combined sources
Beta strandi142 – 15716Combined sources
Beta strandi161 – 1644Combined sources
Beta strandi172 – 1765Combined sources
Beta strandi183 – 1897Combined sources
Helixi196 – 21217Combined sources
Turni220 – 2234Combined sources
Beta strandi224 – 23310Combined sources
Beta strandi239 – 2413Combined sources
Helixi247 – 26317Combined sources
Beta strandi277 – 2793Combined sources
Beta strandi304 – 3063Combined sources
Beta strandi309 – 3113Combined sources
Helixi313 – 3219Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4N0HX-ray1.95B16-329[»]
4N0IX-ray2.00B16-329[»]
ProteinModelPortaliP33893.
SMRiP33893. Positions 27-523.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GatB/GatE family. GatB subfamily.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000016644.
HOGENOMiHOG000223742.
InParanoidiP33893.
KOiK02434.
OMAiMRVDVNL.
OrthoDBiEOG7ZSJ2V.

Family and domain databases

HAMAPiMF_00121. GatB.
InterProiIPR004413. Apn/Gln-ADT_bsu.
IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 1 hit.
PfamiPF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00133. gatB. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33893-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRLARFYSL ARTKAIHSHG APFRPEYALK CGLEIHTQLN TKNKLFSQST
60 70 80 90 100
NSATSLVDAP NHHTSYYDIA LPGTQPVLNL EAILFAMKLS LALGSQVNSI
110 120 130 140 150
SQFDRKHYFY GDQPQGYQLT QHYRPFARGG KINLSKELDD IDESAKEIGI
160 170 180 190 200
LQLQIEQDTG KSHYTETDKD VITLVDLNRS NVPLIELVTK PDFSDIKQVR
210 220 230 240 250
AFIKKYQNLV RHLHISSGDL ETGAMRVDVN LSINEYARVE LKNLPNTSSI
260 270 280 290 300
INAIKYEYQR QVELISVGDT SSLMEPETRG WTGSSTVKLR SKETTIDYRY
310 320 330 340 350
MPDPELPYIN LAPDVISGVR GLMPQLPDDI MRILMKKPYQ LSLKDAKILT
360 370 380 390 400
YNSNQNDMYN HEALRSYYLD TFREFSKLAG ERSNAKLPTN WIIHEFLGDL
410 420 430 440 450
NKLQIPLAKA KEILPPPVFA QFLKLLHEEV ISATSGKMLL FHILENFEQS
460 470 480 490 500
NCQDLSIPDF SKLIEKFELH AINQVDPQEL MDLCNDVIAQ HTDDTFIRNL
510 520 530 540
VTGKKKSSLK FLIGQGMRRS QGRIKANEFE KKFKEILNIQ W
Length:541
Mass (Da):61,869
Last modified:July 27, 2011 - v3
Checksum:iF426E82F1532B07D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti415 – 4151P → A in CAA56028 (PubMed:7502586).Curated
Sequence conflicti415 – 4151P → A in CAA84901 (PubMed:7813418).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22072 Genomic DNA. Translation: AAC37508.1.
X79489 Genomic DNA. Translation: CAA56028.1.
Z35841 Genomic DNA. Translation: CAA84901.1.
BK006936 Genomic DNA. Translation: DAA07043.2.
PIRiS45428.
RefSeqiNP_009473.2. NM_001178320.2.

Genome annotation databases

EnsemblFungiiYBL080C; YBL080C; YBL080C.
GeneIDi852198.
KEGGisce:YBL080C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22072 Genomic DNA. Translation: AAC37508.1.
X79489 Genomic DNA. Translation: CAA56028.1.
Z35841 Genomic DNA. Translation: CAA84901.1.
BK006936 Genomic DNA. Translation: DAA07043.2.
PIRiS45428.
RefSeqiNP_009473.2. NM_001178320.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4N0HX-ray1.95B16-329[»]
4N0IX-ray2.00B16-329[»]
ProteinModelPortaliP33893.
SMRiP33893. Positions 27-523.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32623. 4 interactions.
DIPiDIP-5011N.
IntActiP33893. 1 interaction.
MINTiMINT-508745.

Proteomic databases

MaxQBiP33893.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBL080C; YBL080C; YBL080C.
GeneIDi852198.
KEGGisce:YBL080C.

Organism-specific databases

EuPathDBiFungiDB:YBL080C.
SGDiS000000176. PET112.

Phylogenomic databases

GeneTreeiENSGT00390000016644.
HOGENOMiHOG000223742.
InParanoidiP33893.
KOiK02434.
OMAiMRVDVNL.
OrthoDBiEOG7ZSJ2V.

Enzyme and pathway databases

BioCyciYEAST:G3O-28971-MONOMER.

Miscellaneous databases

NextBioi970688.
PROiP33893.

Family and domain databases

HAMAPiMF_00121. GatB.
InterProiIPR004413. Apn/Gln-ADT_bsu.
IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 1 hit.
PfamiPF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00133. gatB. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "PET112, a Saccharomyces cerevisiae nuclear gene required to maintain rho+ mitochondrial DNA."
    Mulero J.J., Rosenthal J.K., Fox T.D.
    Curr. Genet. 25:299-304(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II."
    Obermaier B., Gassenhuber J., Piravandi E., Domdey H.
    Yeast 11:1103-1112(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 415.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  7. "Yeast mitochondrial Gln-tRNA(Gln) is generated by a GatFAB-mediated transamidation pathway involving Arc1p-controlled subcellular sorting of cytosolic GluRS."
    Frechin M., Senger B., Braye M., Kern D., Martin R.P., Becker H.D.
    Genes Dev. 23:1119-1130(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HER2 AND YGR102C, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiGATB_YEAST
AccessioniPrimary (citable) accession number: P33893
Secondary accession number(s): D6VPS3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1390 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.