P33893 (GATB_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial
Short name=Glu-AdT subunit B
EC=6.3.5.-

Alternative name(s):
Cytochrome oxidase assembly factor PET112

Gene names

Name:	PET112
Ordered Locus Names:	YBL080C
ORF Names:	YBL0724

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	541 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). Ref.7
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.
Subunit structure	Subunit of the heterotrimeric GatFAB amidotransferase(AdT) complex, composed of A (HER2), B (PET112) and F (YGR102C) subunits.
Subcellular location	Mitochondrion Ref.6 Ref.7.
Miscellaneous	Present with 1390 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the GatB/GatE family. GatB subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glutaminyl-tRNAGln biosynthesis via transamidation Inferred from direct assay Ref.7. Source: SGD mitochondrial translation Inferred from mutant phenotype Ref.1. Source: SGD
Cellular component	glutamyl-tRNA(Gln) amidotransferase complex Inferred from direct assay Ref.7. Source: SGD mitochondrion Inferred from direct assay Ref.6Ref.7. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Inferred from direct assay Ref.7. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 541		Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial		PRO_0000010712
Experimental info
	Sequence conflict	415	1	P → A in CAA56028. Ref.2
	Sequence conflict	415	1	P → A in CAA84901. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P33893 [UniParc]. Last modified July 27, 2011. Version 3. Checksum: F426E82F1532B07D	FASTA	541	61,869
	10 20 30 40 50 60 MLRLARFYSL ARTKAIHSHG APFRPEYALK CGLEIHTQLN TKNKLFSQST NSATSLVDAP 70 80 90 100 110 120 NHHTSYYDIA LPGTQPVLNL EAILFAMKLS LALGSQVNSI SQFDRKHYFY GDQPQGYQLT 130 140 150 160 170 180 QHYRPFARGG KINLSKELDD IDESAKEIGI LQLQIEQDTG KSHYTETDKD VITLVDLNRS 190 200 210 220 230 240 NVPLIELVTK PDFSDIKQVR AFIKKYQNLV RHLHISSGDL ETGAMRVDVN LSINEYARVE 250 260 270 280 290 300 LKNLPNTSSI INAIKYEYQR QVELISVGDT SSLMEPETRG WTGSSTVKLR SKETTIDYRY 310 320 330 340 350 360 MPDPELPYIN LAPDVISGVR GLMPQLPDDI MRILMKKPYQ LSLKDAKILT YNSNQNDMYN 370 380 390 400 410 420 HEALRSYYLD TFREFSKLAG ERSNAKLPTN WIIHEFLGDL NKLQIPLAKA KEILPPPVFA 430 440 450 460 470 480 QFLKLLHEEV ISATSGKMLL FHILENFEQS NCQDLSIPDF SKLIEKFELH AINQVDPQEL 490 500 510 520 530 540 MDLCNDVIAQ HTDDTFIRNL VTGKKKSSLK FLIGQGMRRS QGRIKANEFE KKFKEILNIQ W

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PET112, a Saccharomyces cerevisiae nuclear gene required to maintain rho+ mitochondrial DNA." Mulero J.J., Rosenthal J.K., Fox T.D. Curr. Genet. 25:299-304(1994) [PubMed: 8082172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II." Obermaier B., Gassenhuber J., Piravandi E., Domdey H. Yeast 11:1103-1112(1995) [PubMed: 7502586] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 415. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed: 14576278] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
[7]	"Yeast mitochondrial Gln-tRNA(Gln) is generated by a GatFAB-mediated transamidation pathway involving Arc1p-controlled subcellular sorting of cytosolic GluRS." Frechin M., Senger B., Braye M., Kern D., Martin R.P., Becker H.D. Genes Dev. 23:1119-1130(2009) [PubMed: 19417106] [Abstract] Cited for: FUNCTION, INTERACTION WITH HER2 AND YGR102C, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L22072 Genomic DNA. Translation: AAC37508.1. X79489 Genomic DNA. Translation: CAA56028.1. Z35841 Genomic DNA. Translation: CAA84901.1. BK006936 Genomic DNA. Translation: DAA07043.2.
PIR	S45428.
RefSeq	NP_009473.2. NM_001178320.2.
3D structure databases
ProteinModelPortal	P33893.
SMR	P33893. Positions 25-540.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5011N.
IntAct	P33893. 2 interactions.
MINT	MINT-508745.
STRING	P33893.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	852198.
KEGG	sce:YBL080C.
NMPDR	fig|4932.3.peg.159.
Organism-specific databases
CYGD	YBL080c.
SGD	S000000176. PET112.
Phylogenomic databases
eggNOG	fuNOG07865.
GeneTree	EFGT00050000006142.
HOGENOM	HBG395951.
OMA	IHTQLNT.
OrthoDB	EOG473T0X.
Gene expression databases
ArrayExpress	P33893.
Genevestigator	P33893.
GermOnline	YBL080C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E. IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat. IPR018027. Asn/Gln_amidotransferase. IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel. IPR004413. Gln-tRNA_amidoTrfase_bsu. IPR017958. Gln-tRNA_amidoTrfase_suB_CS. [Graphical view]
KO	K02434.
PANTHER	PTHR11659. GatB. 1 hit.
Pfam	PF02934. GatB_N. 1 hit. PF02637. GatB_Yqey. 1 hit. [Graphical view]
SMART	SM00845. GatB_Yqey. 1 hit. [Graphical view]
SUPFAM	SSF89095. GatB_Yqey. 1 hit.
TIGRFAMs	TIGR00133. GatB. 1 hit.
PROSITE	PS01234. GATB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	970688.

Entry information

Entry name

GATB_YEAST

Accession

Primary (citable) accession number: P33893
Secondary accession number(s): D6VPS3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	July 27, 2011
Last modified:	December 14, 2011
This is version 101 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program