##gff-version 3 P33892 UniProtKB Chain 1 2672 . . . ID=PRO_0000087444;Note=EIF-2-alpha kinase activator GCN1 P33892 UniProtKB Repeat 5 42 . . . Note=HEAT 1;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 79 117 . . . Note=HEAT 2;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 174 211 . . . Note=HEAT 3;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 227 267 . . . Note=HEAT 4;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 329 366 . . . Note=HEAT 5;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 372 410 . . . Note=HEAT 6;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 509 549 . . . Note=HEAT 7;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 611 648 . . . Note=HEAT 8;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 706 745 . . . Note=HEAT 9;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 902 932 . . . Note=HEAT 10;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 933 970 . . . Note=HEAT 11;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 975 994 . . . Note=HEAT 12%3B degenerate;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 995 1030 . . . Note=HEAT 13%3B degenerate;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 1031 1067 . . . Note=HEAT 14;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 1099 1138 . . . Note=HEAT 15;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 1185 1224 . . . Note=HEAT 16;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 1243 1281 . . . Note=HEAT 17;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 1284 1321 . . . Note=HEAT 18;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 1363 1401 . . . Note=HEAT 19;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 1405 1442 . . . Note=HEAT 20;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 1444 1480 . . . Note=HEAT 21;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 1484 1521 . . . Note=HEAT 22;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 1523 1559 . . . Note=HEAT 23;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 1561 1598 . . . Note=HEAT 24;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 1603 1640 . . . Note=HEAT 25;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 1641 1679 . . . Note=HEAT 26;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 1681 1717 . . . Note=HEAT 27;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 1721 1758 . . . Note=HEAT 28;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 1760 1796 . . . Note=HEAT 29;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 1825 1862 . . . Note=HEAT 30;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 1863 1903 . . . Note=HEAT 31;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 1905 1942 . . . Note=HEAT 32;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 1947 1984 . . . Note=HEAT 33;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 1985 2024 . . . Note=HEAT 34;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 2026 2055 . . . Note=HEAT 35;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 2057 2095 . . . Note=HEAT 36;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 2097 2134 . . . Note=HEAT 37;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 2138 2175 . . . Note=HEAT 38;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 2206 2243 . . . Note=HEAT 39;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 2250 2286 . . . Note=HEAT 40;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 2290 2328 . . . Note=HEAT 41;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 2347 2384 . . . Note=HEAT 42;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 2392 2429 . . . Note=HEAT 43;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 2450 2487 . . . Note=HEAT 44;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Repeat 2506 2546 . . . Note=HEAT 45;Ontology_term=ECO:0000255;evidence=ECO:0000255 P33892 UniProtKB Region 1330 1641 . . . Note=EF3-like region;Ontology_term=ECO:0000303;evidence=ECO:0000303|PubMed:9234705;Dbxref=PMID:9234705 P33892 UniProtKB Region 2207 2356 . . . Note=RWDBD region;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:28526057;Dbxref=PMID:28526057 P33892 UniProtKB Mutagenesis 757 758 . . . Note=Does not inhibit interaction with GCN20%2C reduces ribosome binding%2C ribosome binding by GCN20%2C weakly impairs eIF-2-alpha phosphorylation but strongly impairs eIF-2-alpha phosphorylation in a YEF3-dependent manner%3B when associated with A-762%3B A-765%3B A-775%3B 777-A-A-778%3B 782-A-A-783%3B 786-A-A-787 and A-790. KK->AA%2CDD;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:15722345,ECO:0000269|PubMed:22888004;Dbxref=PMID:15722345,PMID:22888004 P33892 UniProtKB Mutagenesis 762 762 . . . Note=Does not inhibit interaction with GCN20%2C reduces ribosome binding%2C ribosome binding by GCN20%2C weakly impairs eIF-2-alpha phosphorylation but strongly impairs eIF-2-alpha phosphorylation in a YEF3-dependent manner%3B when associated with A-757-758-A%3B A-765%3B A-775%3B 777-A-A-778%3B 782-A-A-783%3B 786-A-A-787 and A-790. K->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:15722345,ECO:0000269|PubMed:22888004;Dbxref=PMID:15722345,PMID:22888004 P33892 UniProtKB Mutagenesis 765 765 . . . Note=Does not inhibit interaction with GCN20%2C reduces ribosome binding%2C ribosome binding by GCN20%2C weakly impairs eIF-2-alpha phosphorylation but strongly impairs eIF-2-alpha phosphorylation in a YEF3-dependent manner%3B when associated with A-757-758-A%3B A-762%3B A-775%3B 777-A-A-778%3B 782-A-A-783%3B 786-A-A-787 and A-790. K->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:15722345,ECO:0000269|PubMed:22888004;Dbxref=PMID:15722345,PMID:22888004 P33892 UniProtKB Mutagenesis 775 775 . . . Note=Does not inhibit interaction with GCN20%2C reduces ribosome binding%2C ribosome binding by GCN20%2C weakly impairs eIF-2-alpha phosphorylation but strongly impairs eIF-2-alpha phosphorylation in a YEF3-dependent manner%3B when associated with A-757-758-A%3B A-762%3B A-765%3B 777-A-A-778%3B 782-A-A-783%3B 786-A-A-787 and A-790. K->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:15722345,ECO:0000269|PubMed:22888004;Dbxref=PMID:15722345,PMID:22888004 P33892 UniProtKB Mutagenesis 777 778 . . . Note=Does not inhibit interaction with GCN20%2C reduces ribosome binding%2C ribosome binding by GCN20%2C weakly impairs eIF-2-alpha phosphorylation but strongly impairs eIF-2-alpha phosphorylation in a YEF3-dependent manner%3B when associated with A-757-758-A%3B A-762%3B A-765%3B A-775%3B 782-A-A-783%3B 786-A-A-787 and A-790. RK->AA;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:15722345,ECO:0000269|PubMed:22888004;Dbxref=PMID:15722345,PMID:22888004 P33892 UniProtKB Mutagenesis 782 783 . . . Note=Does not inhibit interaction with GCN20%2C reduces ribosome binding%2C ribosome binding by GCN20%2C weakly impairs eIF-2-alpha phosphorylation but strongly impairs eIF-2-alpha phosphorylation in a YEF3-dependent manner%3B when associated with A-757-758-A%3B A-762%3B A-765%3B A-775%3B 777-A-A-778%3B 786-A-A-787 and A-790. KK->AA;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:15722345,ECO:0000269|PubMed:22888004;Dbxref=PMID:15722345,PMID:22888004 P33892 UniProtKB Mutagenesis 786 787 . . . Note=Does not inhibit interaction with GCN20%2C reduces ribosome binding%2C ribosome binding by GCN20%2C weakly impairs eIF-2-alpha phosphorylation but strongly impairs eIF-2-alpha phosphorylation in a YEF3-dependent manner%3B when associated with A-757-758-A%3B A-762%3B A-765%3B A-775%3B 777-A-A-778%3B 782-A-A-783 and A-790. KK->AA;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:15722345,ECO:0000269|PubMed:22888004;Dbxref=PMID:15722345,PMID:22888004 P33892 UniProtKB Mutagenesis 790 790 . . . Note=Does not inhibit interaction with GCN20%2C reduces ribosome binding%2C ribosome binding by GCN20%2C weakly impairs eIF-2-alpha phosphorylation but strongly impairs eIF-2-alpha phosphorylation in a YEF3-dependent manner%3B when associated with A-757-758-A%3B A-762%3B A-765%3B A-775%3B 777-A-A-778%3B 782-A-A-783 and 786-A-A-787. K->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:15722345,ECO:0000269|PubMed:22888004;Dbxref=PMID:15722345,PMID:22888004 P33892 UniProtKB Mutagenesis 1444 1444 . . . Note=Decreases interaction with GCN20 and polysomal association. G->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:9234705;Dbxref=PMID:9234705 P33892 UniProtKB Mutagenesis 1458 1458 . . . Note=Does not inhibit interaction with GCN20%2C reduces ribosome binding%2C ribosome binding by GCN20 and strongly impairs eIF-2-alpha phosphorylation%3B when associated with 1461-A--A-1465. E->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15722345;Dbxref=PMID:15722345 P33892 UniProtKB Mutagenesis 1461 1465 . . . Note=Does not inhibit interaction with GCN20%2C reduces ribosome binding%2C ribosome binding by GCN20 and strongly impairs eIF-2-alpha phosphorylation%3B when associated with A-1458. WRTKR->AAAAA;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15722345;Dbxref=PMID:15722345 P33892 UniProtKB Mutagenesis 2224 2224 . . . Note=Does not affect ability to activate GCN2. N->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36441697;Dbxref=PMID:36441697 P33892 UniProtKB Mutagenesis 2227 2227 . . . Note=Does not affect ability to activate GCN2. R->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36441697;Dbxref=PMID:36441697 P33892 UniProtKB Mutagenesis 2245 2245 . . . Note=Does not affect ability to activate GCN2. N->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36441697;Dbxref=PMID:36441697 P33892 UniProtKB Mutagenesis 2247 2247 . . . Note=Increased ability to activate GCN2. K->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36441697;Dbxref=PMID:36441697 P33892 UniProtKB Mutagenesis 2251 2251 . . . Note=Does not affect ability to activate GCN2. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36441697;Dbxref=PMID:36441697 P33892 UniProtKB Mutagenesis 2259 2259 . . . Note=Decreases interaction with GCN2 and YIH1 but not with GCN20 and ribosomes. R->A;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:11101534,ECO:0000269|PubMed:15126500,ECO:0000269|PubMed:21239490,ECO:0000269|PubMed:36441697;Dbxref=PMID:11101534,PMID:15126500,PMID:21239490,PMID:36441697 P33892 UniProtKB Mutagenesis 2261 2261 . . . Note=Increased ability to activate GCN2. V->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36441697;Dbxref=PMID:36441697 P33892 UniProtKB Mutagenesis 2261 2261 . . . Note=Does not affect ability to activate GCN2. V->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36441697;Dbxref=PMID:36441697 P33892 UniProtKB Mutagenesis 2263 2263 . . . Note=Increased ability to activate GCN2. E->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36441697;Dbxref=PMID:36441697 P33892 UniProtKB Mutagenesis 2264 2264 . . . Note=Increased ability to activate GCN2. R->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36441697;Dbxref=PMID:36441697 P33892 UniProtKB Mutagenesis 2270 2270 . . . Note=Impaired ability to activate GCN2. K->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36441697;Dbxref=PMID:36441697 P33892 UniProtKB Mutagenesis 2289 2289 . . . Note=Impaired ability to activate GCN2. R->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36441697;Dbxref=PMID:36441697 P33892 UniProtKB Mutagenesis 2291 2291 . . . Note=Does not affect ability to activate GCN2. F->E;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36441697;Dbxref=PMID:36441697 P33892 UniProtKB Mutagenesis 2291 2291 . . . Note=Does not interact with GCN2%2C impairs eIF-2-alpha phosphorylation and fails to derepress GCN4 translation in amino acid-starved cells. F->L;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11350982;Dbxref=PMID:11350982 P33892 UniProtKB Mutagenesis 2294 2294 . . . Note=Increased ability to activate GCN2. Q->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36441697;Dbxref=PMID:36441697 P33892 UniProtKB Mutagenesis 2294 2294 . . . Note=Does not affect ability to activate GCN2. Q->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36441697;Dbxref=PMID:36441697 P33892 UniProtKB Mutagenesis 2297 2297 . . . Note=Impaired ability to activate GCN2. R->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36441697;Dbxref=PMID:36441697 P33892 UniProtKB Mutagenesis 2301 2301 . . . Note=Impaired ability to activate GCN2. K->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36441697;Dbxref=PMID:36441697 P33892 UniProtKB Mutagenesis 2304 2304 . . . Note=Does not interact with GCN2. S->P;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11350982;Dbxref=PMID:11350982 P33892 UniProtKB Mutagenesis 2305 2305 . . . Note=Does not affect ability to activate GCN2. D->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36441697;Dbxref=PMID:36441697 P33892 UniProtKB Mutagenesis 2309 2309 . . . Note=Does not affect ability to activate GCN2. E->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36441697;Dbxref=PMID:36441697 P33892 UniProtKB Mutagenesis 2330 2330 . . . Note=Does not affect ability to activate GCN2. D->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36441697;Dbxref=PMID:36441697 P33892 UniProtKB Mutagenesis 2335 2335 . . . Note=Does not affect ability to activate GCN2. E->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36441697;Dbxref=PMID:36441697 P33892 UniProtKB Mutagenesis 2353 2353 . . . Note=Does not interact with GCN2. L->P;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11350982;Dbxref=PMID:11350982 P33892 UniProtKB Mutagenesis 2382 2382 . . . Note=Does not affect ability to activate GCN2. E->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36441697;Dbxref=PMID:36441697