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P33892 (GCN1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Translational activator GCN1
Gene names
Name:GCN1
Ordered Locus Names:YGL195W
ORF Names:G1318
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length2672 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a translation activator that mediates translational control by regulating GCN2 kinase activity. Translational activator of GCN4. May be involved in sensing charged tRNA and stimulating the kinase activity of GCN2 in amino acid-starved cells. Required in vivo for the phosphorylation of eIF-2-alpha on 'Ser-52' by the protein kinase GCN2.

Subunit structure

Component of a heteromeric complex that includes GCN1 and GCN20. Interacts with YIH1; preventing the interaction with GCN2 protein kinase. Interacts with GIR2. Ref.6 Ref.8

Miscellaneous

Present with 7330 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the GCN1 family.

Contains 20 HEAT repeats.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

GCN20P435354EBI-7442,EBI-7423

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 26722672Translational activator GCN1
PRO_0000087444

Regions

Repeat932 – 97039HEAT 1
Repeat1030 – 106738HEAT 2
Repeat1099 – 113840HEAT 3
Repeat1243 – 128139HEAT 4
Repeat1284 – 132138HEAT 5
Repeat1405 – 144238HEAT 6
Repeat1484 – 152138HEAT 7
Repeat1523 – 155937HEAT 8
Repeat1561 – 159838HEAT 9
Repeat1603 – 164038HEAT 10
Repeat1641 – 167939HEAT 11
Repeat1721 – 175838HEAT 12
Repeat1760 – 179637HEAT 13
Repeat1862 – 190342HEAT 14
Repeat1905 – 194238HEAT 15
Repeat1947 – 198438HEAT 16
Repeat1985 – 202440HEAT 17
Repeat2097 – 213438HEAT 18
Repeat2290 – 232839HEAT 19
Repeat2347 – 238438HEAT 20

Sequences

Sequence LengthMass (Da)Tools
P33892 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 980FDD03753E9D1C

FASTA2,672296,698
        10         20         30         40         50         60 
MTAILNWEDI SPVLEKGTRE SHVSKRVPFL QDISQLVRQE TLEKPQLSEI AFVLLNTFTI 

        70         80         90        100        110        120 
YEDNRSKSLV TSILLDILNL EPCLLENFIR FISDVVISNP ATKAVADYLN LLDWINSFLI 

       130        140        150        160        170        180 
FVSHNSNLFE EYIPKLLVAH SYATFGVETI LDNQEEGKKS QDKQNQHRKR IRYCIFQTTV 

       190        200        210        220        230        240 
KAFLKCLKDN DDSISFMKIS IKTVLESYSK LKITSVGVVM IMGALTQAAL QLLSRQPALH 

       250        260        270        280        290        300 
SVLKENSAEK YCEYLGKEVF LGKNPPSSFC LEIGLKPFLK EFVSQELFIK FFIPNIEKAV 

       310        320        330        340        350        360 
LRSPEVGFSI LSELYAGVSP EKVNLLNAFA SSKLINQYFS SFKSSKEVVR SVSLQSMIIL 

       370        380        390        400        410        420 
LRKISNTDTT LEDLTKLIDE IFKNIKSNLN ADYKSLISKI LIEIPLTHYE VSEKICKGLS 

       430        440        450        460        470        480 
PYIGKEGNEA ALTLMLNAFF VHYFSLGKPI EDLDKIISAG FADKKPALKK CWFAAFLNNS 

       490        500        510        520        530        540 
NAASEEVILN FIDGCLEFVK DSIIHYQTHG HACILASIEF TNKILALDNT ELNDRVMQLI 

       550        560        570        580        590        600 
ETLPENSSIG DAILTAALST ELSIENRIHA VNLLQELFYK KPEFIGFSVI DAIERRMRVQ 

       610        620        630        640        650        660 
ELIPQQNTSF KYVTSVLLAI TSELPDKEAS IKVLINALVI AQWNIFNIKN GWAGLVLRAR 

       670        680        690        700        710        720 
LDPAEVVKEH ASVIMEKILE ITGSCEWIDT IYGACGLQAA AYAAFIQPNE FTPILCKTIE 

       730        740        750        760        770        780 
ADLTADDFSR LSEEDFEIFA GEEGVLVVDV LEESMNKKLS NKNSKEYETL MWEQKIRKEQ 

       790        800        810        820        830        840 
AKKNVKKLSK EEQELVNEQL AKESAVRSHV SEISTRLKRG IRLVSELSKA ACLVQNGIAT 

       850        860        870        880        890        900 
WFPLAVTKLL YLCSEPNISK LTEDVNNVFL QLSQNVSERL GNIRLFLGLA TLRVHNANGI 

       910        920        930        940        950        960 
SQDYLQEPLV ELLTRVLFRI KFVSNQAAID SISLTYILPL LINVLEKGKA IALKNADKPV 

       970        980        990       1000       1010       1020 
VKAEFVEEDE EEEHLLLAME IISVHAEAFE DPSIPRISIV EVLLSLLSLP SKAKIAKDCF 

      1030       1040       1050       1060       1070       1080 
NALCQSISVA PNQEDLDMIL SNLLSPNQFV RSTILETLDN EFELEPFMKY SPEVFICRFD 

      1090       1100       1110       1120       1130       1140 
SDPSNREIAD FIWEFNKFVV NDELLKSLFP LFNQDDSGLR LFAANAYAFG AVSLFTSEEN 

      1150       1160       1170       1180       1190       1200 
SSKDYLNDLL NFYKEKAKPL EPILDQFGLV LVSASEQKDP WQGRSTVAIT LKIMAKAFSA 

      1210       1220       1230       1240       1250       1260 
EDDTVVNIIK FLVDDGGLVD REPIVRQEMK EAGVELITLH GSQNSKDLIP IFEEALSSST 

      1270       1280       1290       1300       1310       1320 
DSALKENVII LYGTLARHLQ QSDARIHTII ERLLSTLDTP SADIQQAVSA CIAPLVFQFK 

      1330       1340       1350       1360       1370       1380 
QKVGDYLGIL MEKLLNPTVA SSMRKGAAWG IAGLVKGYGI SALSEFDIIR NLIEAAEDKK 

      1390       1400       1410       1420       1430       1440 
EPKRRESVGF CFQYLSESLG KFFEPYVIEI LPNILKNLGD AVPEVRDATA RATKAIMAHT 

      1450       1460       1470       1480       1490       1500 
TGYGVKKLIP VAVSNLDEIA WRTKRGSVQL LGNMAYLDPT QLSASLSTIV PEIVGVLNDS 

      1510       1520       1530       1540       1550       1560 
HKEVRKAADE SLKRFGEVIR NPEIQKLVPV LLQAIGDPTK YTEEALDSLI QTQFVHYIDG 

      1570       1580       1590       1600       1610       1620 
PSLALIIHII HRGMHDRSAN IKRKACKIVG NMAILVDTKD LIPYLQQLID EVEIAMVDPV 

      1630       1640       1650       1660       1670       1680 
PNTRATAARA LGALVERLGE EQFPDLIPRL LDTLSDESKS GDRLGSAQAL AEVISGLGLT 

      1690       1700       1710       1720       1730       1740 
KLDEMLPTIL AGVTNFRAYI REGFMPLLLF LPVCFGSQFA PYINQIIQPI LSGLADNDEN 

      1750       1760       1770       1780       1790       1800 
IRDTALKAGK LIVKNYATKA VDLLLPELER GMFDENDRIR LSSVQLTGEL LFQVTGISSR 

      1810       1820       1830       1840       1850       1860 
NEFSEEDGDH NGEFSGKLVD VLGQDRRDRI LAALFVCRND TSGIVRATTV DIWKALVPNT 

      1870       1880       1890       1900       1910       1920 
PRAVKEILPT LTGMIVTHLA SSSNVLRNIA AQTLGDLVRR VGGNALSQLL PSLEESLIET 

      1930       1940       1950       1960       1970       1980 
SNSDSRQGVC IALYELIESA STETISQFQS TIVNIIRTAL IDESATVREA AALSFDVFQD 

      1990       2000       2010       2020       2030       2040 
VVGKTAVDEV LPYLLHMLES SDNSDFALLG LQEIMSKKSD VIFPILIPTL LAPPIDAFRA 

      2050       2060       2070       2080       2090       2100 
SALGSLAEVA GSALYKRLSI IINALVDAII GTSEDESTKG ALELALDRVF LSVNDDEGLH 

      2110       2120       2130       2140       2150       2160 
PLLQQIMSLL KSDNIEKRIA VLERLPNFFD KTVLDFDVYI PNFVSHAILS LDDEDQRVVN 

      2170       2180       2190       2200       2210       2220 
GNFNALSTLL KKVDKPTLEK LVKPAKQSLA LTGRQGQDVA AFKLPRGPNC VLPIFLHGLM 

      2230       2240       2250       2260       2270       2280 
YGSNDEREES ALAIADVVSK TPAANLKPFV SVITGPLIRV VGERFSSDIK AAILFALNVL 

      2290       2300       2310       2320       2330       2340 
FIKIPMFLRP FIPQLQRTFV KSLSDATNET LRLRAAKALG ALIEHQPRVD PLVIELVTGA 

      2350       2360       2370       2380       2390       2400 
KQATDEGVKT AMLKALLEVI MKAGSKLNEN SKTNIVNLVE EEMLGSNDKL AVAYAKLIGS 

      2410       2420       2430       2440       2450       2460 
LSEILSNDEA HKILQDKVLN ADLDGETGKF AILTLNSFLK DAPTHIFNTG LIDEFVSYIL 

      2470       2480       2490       2500       2510       2520 
NAIRSPDVYF GENGTIAAGK LLLLEGEKRS PFVKKDAAEP FKIGDENINL LINELSKAVL 

      2530       2540       2550       2560       2570       2580 
QPASNSTDVR RLALVVIRTL ARFKFDECIK QYFDVVGPSV FSCLRDPVIP IKLAAEKAYL 

      2590       2600       2610       2620       2630       2640 
ALFKLVEEDD MHTFNEWFAK ISDRGNSIET VTGTTIQLRS VGDYTKRVGK RLANVERERI 

      2650       2660       2670 
AAGGDAETMF SDRFEDEREI WAVGGVELTT DI 

« Hide

References

« Hide 'large scale' references
[1]"GCN1, a translational activator of GCN4 in Saccharomyces cerevisiae, is required for phosphorylation of eukaryotic translation initiation factor 2 by protein kinase GCN2."
Marton M.J., Crouch D., Hinnebusch A.G.
Mol. Cell. Biol. 13:3541-3556(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae."
Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V.
Yeast 13:55-64(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"YIH1 is an actin-binding protein that inhibits protein kinase GCN2 and impairs general amino acid control when overexpressed."
Sattlegger E., Swanson M.J., Ashcraft E.A., Jennings J.L., Fekete R.A., Link A.J., Hinnebusch A.G.
J. Biol. Chem. 279:29952-29962(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH YIH1.
[7]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[8]"Saccharomyces cerevisiae Rbg1 protein and its binding partner Gir2 interact on polyribosomes with Gcn1."
Wout P.K., Sattlegger E., Sullivan S.M., Maddock J.R.
Eukaryot. Cell 8:1061-1071(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GIR2.
[9]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L12467 Genomic DNA. Translation: AAA34635.1.
X91837 Genomic DNA. Translation: CAA62949.1.
Z72717 Genomic DNA. Translation: CAA96907.1.
BK006941 Genomic DNA. Translation: DAA07919.1.
PIRA48126.
RefSeqNP_011320.3. NM_001181060.3.

3D structure databases

ProteinModelPortalP33892.
SMRP33892. Positions 1404-1674.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33062. 285 interactions.
DIPDIP-2344N.
IntActP33892. 128 interactions.
MINTMINT-634438.
STRING4932.YGL195W.

Proteomic databases

MaxQBP33892.
PaxDbP33892.
PeptideAtlasP33892.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL195W; YGL195W; YGL195W.
GeneID852680.
KEGGsce:YGL195W.

Organism-specific databases

CYGDYGL195w.
SGDS000003163. GCN1.

Phylogenomic databases

eggNOGNOG325174.
GeneTreeENSGT00390000011675.
HOGENOMHOG000213714.
OMADEIAWRT.
OrthoDBEOG7FJH7S.

Enzyme and pathway databases

BioCycYEAST:G3O-30676-MONOMER.

Gene expression databases

GenevestigatorP33892.

Family and domain databases

Gene3D1.25.10.10. 8 hits.
InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR022716. DUF3554.
IPR026827. ECM29/GCN1.
IPR021133. HEAT_type_2.
[Graphical view]
PANTHERPTHR23346. PTHR23346. 1 hit.
PfamPF12074. DUF3554. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 8 hits.
PROSITEPS50077. HEAT_REPEAT. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio971993.
PROP33892.

Entry information

Entry nameGCN1_YEAST
AccessionPrimary (citable) accession number: P33892
Secondary accession number(s): D6VTV8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 11, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families