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P33892

- GCN1_YEAST

UniProt

P33892 - GCN1_YEAST

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Protein

Translational activator GCN1

Gene
GCN1, YGL195W, G1318
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Acts as a translation activator that mediates translational control by regulating GCN2 kinase activity. Translational activator of GCN4. May be involved in sensing charged tRNA and stimulating the kinase activity of GCN2 in amino acid-starved cells. Required in vivo for the phosphorylation of eIF-2-alpha on 'Ser-52' by the protein kinase GCN2.

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. regulation of translational elongation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Translation regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-30676-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Translational activator GCN1
Gene namesi
Name:GCN1
Ordered Locus Names:YGL195W
ORF Names:G1318
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL195w.
SGDiS000003163. GCN1.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: SGD
  2. cytosolic ribosome Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 26722672Translational activator GCN1PRO_0000087444Add
BLAST

Proteomic databases

MaxQBiP33892.
PaxDbiP33892.
PeptideAtlasiP33892.

Expressioni

Gene expression databases

GenevestigatoriP33892.

Interactioni

Subunit structurei

Component of a heteromeric complex that includes GCN1 and GCN20. Interacts with YIH1; preventing the interaction with GCN2 protein kinase. Interacts with GIR2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GCN20P435354EBI-7442,EBI-7423

Protein-protein interaction databases

BioGridi33062. 288 interactions.
DIPiDIP-2344N.
IntActiP33892. 128 interactions.
MINTiMINT-634438.
STRINGi4932.YGL195W.

Structurei

3D structure databases

ProteinModelPortaliP33892.
SMRiP33892. Positions 1404-1674.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati932 – 97039HEAT 1Add
BLAST
Repeati1030 – 106738HEAT 2Add
BLAST
Repeati1099 – 113840HEAT 3Add
BLAST
Repeati1243 – 128139HEAT 4Add
BLAST
Repeati1284 – 132138HEAT 5Add
BLAST
Repeati1405 – 144238HEAT 6Add
BLAST
Repeati1484 – 152138HEAT 7Add
BLAST
Repeati1523 – 155937HEAT 8Add
BLAST
Repeati1561 – 159838HEAT 9Add
BLAST
Repeati1603 – 164038HEAT 10Add
BLAST
Repeati1641 – 167939HEAT 11Add
BLAST
Repeati1721 – 175838HEAT 12Add
BLAST
Repeati1760 – 179637HEAT 13Add
BLAST
Repeati1862 – 190342HEAT 14Add
BLAST
Repeati1905 – 194238HEAT 15Add
BLAST
Repeati1947 – 198438HEAT 16Add
BLAST
Repeati1985 – 202440HEAT 17Add
BLAST
Repeati2097 – 213438HEAT 18Add
BLAST
Repeati2290 – 232839HEAT 19Add
BLAST
Repeati2347 – 238438HEAT 20Add
BLAST

Sequence similaritiesi

Belongs to the GCN1 family.
Contains 20 HEAT repeats.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG325174.
GeneTreeiENSGT00390000011675.
HOGENOMiHOG000213714.
OMAiDEIAWRT.
OrthoDBiEOG7FJH7S.

Family and domain databases

Gene3Di1.25.10.10. 8 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR022716. DUF3554.
IPR026827. ECM29/GCN1.
IPR021133. HEAT_type_2.
[Graphical view]
PANTHERiPTHR23346. PTHR23346. 1 hit.
PfamiPF12074. DUF3554. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 8 hits.
PROSITEiPS50077. HEAT_REPEAT. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33892-1 [UniParc]FASTAAdd to Basket

« Hide

MTAILNWEDI SPVLEKGTRE SHVSKRVPFL QDISQLVRQE TLEKPQLSEI     50
AFVLLNTFTI YEDNRSKSLV TSILLDILNL EPCLLENFIR FISDVVISNP 100
ATKAVADYLN LLDWINSFLI FVSHNSNLFE EYIPKLLVAH SYATFGVETI 150
LDNQEEGKKS QDKQNQHRKR IRYCIFQTTV KAFLKCLKDN DDSISFMKIS 200
IKTVLESYSK LKITSVGVVM IMGALTQAAL QLLSRQPALH SVLKENSAEK 250
YCEYLGKEVF LGKNPPSSFC LEIGLKPFLK EFVSQELFIK FFIPNIEKAV 300
LRSPEVGFSI LSELYAGVSP EKVNLLNAFA SSKLINQYFS SFKSSKEVVR 350
SVSLQSMIIL LRKISNTDTT LEDLTKLIDE IFKNIKSNLN ADYKSLISKI 400
LIEIPLTHYE VSEKICKGLS PYIGKEGNEA ALTLMLNAFF VHYFSLGKPI 450
EDLDKIISAG FADKKPALKK CWFAAFLNNS NAASEEVILN FIDGCLEFVK 500
DSIIHYQTHG HACILASIEF TNKILALDNT ELNDRVMQLI ETLPENSSIG 550
DAILTAALST ELSIENRIHA VNLLQELFYK KPEFIGFSVI DAIERRMRVQ 600
ELIPQQNTSF KYVTSVLLAI TSELPDKEAS IKVLINALVI AQWNIFNIKN 650
GWAGLVLRAR LDPAEVVKEH ASVIMEKILE ITGSCEWIDT IYGACGLQAA 700
AYAAFIQPNE FTPILCKTIE ADLTADDFSR LSEEDFEIFA GEEGVLVVDV 750
LEESMNKKLS NKNSKEYETL MWEQKIRKEQ AKKNVKKLSK EEQELVNEQL 800
AKESAVRSHV SEISTRLKRG IRLVSELSKA ACLVQNGIAT WFPLAVTKLL 850
YLCSEPNISK LTEDVNNVFL QLSQNVSERL GNIRLFLGLA TLRVHNANGI 900
SQDYLQEPLV ELLTRVLFRI KFVSNQAAID SISLTYILPL LINVLEKGKA 950
IALKNADKPV VKAEFVEEDE EEEHLLLAME IISVHAEAFE DPSIPRISIV 1000
EVLLSLLSLP SKAKIAKDCF NALCQSISVA PNQEDLDMIL SNLLSPNQFV 1050
RSTILETLDN EFELEPFMKY SPEVFICRFD SDPSNREIAD FIWEFNKFVV 1100
NDELLKSLFP LFNQDDSGLR LFAANAYAFG AVSLFTSEEN SSKDYLNDLL 1150
NFYKEKAKPL EPILDQFGLV LVSASEQKDP WQGRSTVAIT LKIMAKAFSA 1200
EDDTVVNIIK FLVDDGGLVD REPIVRQEMK EAGVELITLH GSQNSKDLIP 1250
IFEEALSSST DSALKENVII LYGTLARHLQ QSDARIHTII ERLLSTLDTP 1300
SADIQQAVSA CIAPLVFQFK QKVGDYLGIL MEKLLNPTVA SSMRKGAAWG 1350
IAGLVKGYGI SALSEFDIIR NLIEAAEDKK EPKRRESVGF CFQYLSESLG 1400
KFFEPYVIEI LPNILKNLGD AVPEVRDATA RATKAIMAHT TGYGVKKLIP 1450
VAVSNLDEIA WRTKRGSVQL LGNMAYLDPT QLSASLSTIV PEIVGVLNDS 1500
HKEVRKAADE SLKRFGEVIR NPEIQKLVPV LLQAIGDPTK YTEEALDSLI 1550
QTQFVHYIDG PSLALIIHII HRGMHDRSAN IKRKACKIVG NMAILVDTKD 1600
LIPYLQQLID EVEIAMVDPV PNTRATAARA LGALVERLGE EQFPDLIPRL 1650
LDTLSDESKS GDRLGSAQAL AEVISGLGLT KLDEMLPTIL AGVTNFRAYI 1700
REGFMPLLLF LPVCFGSQFA PYINQIIQPI LSGLADNDEN IRDTALKAGK 1750
LIVKNYATKA VDLLLPELER GMFDENDRIR LSSVQLTGEL LFQVTGISSR 1800
NEFSEEDGDH NGEFSGKLVD VLGQDRRDRI LAALFVCRND TSGIVRATTV 1850
DIWKALVPNT PRAVKEILPT LTGMIVTHLA SSSNVLRNIA AQTLGDLVRR 1900
VGGNALSQLL PSLEESLIET SNSDSRQGVC IALYELIESA STETISQFQS 1950
TIVNIIRTAL IDESATVREA AALSFDVFQD VVGKTAVDEV LPYLLHMLES 2000
SDNSDFALLG LQEIMSKKSD VIFPILIPTL LAPPIDAFRA SALGSLAEVA 2050
GSALYKRLSI IINALVDAII GTSEDESTKG ALELALDRVF LSVNDDEGLH 2100
PLLQQIMSLL KSDNIEKRIA VLERLPNFFD KTVLDFDVYI PNFVSHAILS 2150
LDDEDQRVVN GNFNALSTLL KKVDKPTLEK LVKPAKQSLA LTGRQGQDVA 2200
AFKLPRGPNC VLPIFLHGLM YGSNDEREES ALAIADVVSK TPAANLKPFV 2250
SVITGPLIRV VGERFSSDIK AAILFALNVL FIKIPMFLRP FIPQLQRTFV 2300
KSLSDATNET LRLRAAKALG ALIEHQPRVD PLVIELVTGA KQATDEGVKT 2350
AMLKALLEVI MKAGSKLNEN SKTNIVNLVE EEMLGSNDKL AVAYAKLIGS 2400
LSEILSNDEA HKILQDKVLN ADLDGETGKF AILTLNSFLK DAPTHIFNTG 2450
LIDEFVSYIL NAIRSPDVYF GENGTIAAGK LLLLEGEKRS PFVKKDAAEP 2500
FKIGDENINL LINELSKAVL QPASNSTDVR RLALVVIRTL ARFKFDECIK 2550
QYFDVVGPSV FSCLRDPVIP IKLAAEKAYL ALFKLVEEDD MHTFNEWFAK 2600
ISDRGNSIET VTGTTIQLRS VGDYTKRVGK RLANVERERI AAGGDAETMF 2650
SDRFEDEREI WAVGGVELTT DI 2672
Length:2,672
Mass (Da):296,698
Last modified:February 1, 1994 - v1
Checksum:i980FDD03753E9D1C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L12467 Genomic DNA. Translation: AAA34635.1.
X91837 Genomic DNA. Translation: CAA62949.1.
Z72717 Genomic DNA. Translation: CAA96907.1.
BK006941 Genomic DNA. Translation: DAA07919.1.
PIRiA48126.
RefSeqiNP_011320.3. NM_001181060.3.

Genome annotation databases

EnsemblFungiiYGL195W; YGL195W; YGL195W.
GeneIDi852680.
KEGGisce:YGL195W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L12467 Genomic DNA. Translation: AAA34635.1 .
X91837 Genomic DNA. Translation: CAA62949.1 .
Z72717 Genomic DNA. Translation: CAA96907.1 .
BK006941 Genomic DNA. Translation: DAA07919.1 .
PIRi A48126.
RefSeqi NP_011320.3. NM_001181060.3.

3D structure databases

ProteinModelPortali P33892.
SMRi P33892. Positions 1404-1674.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33062. 288 interactions.
DIPi DIP-2344N.
IntActi P33892. 128 interactions.
MINTi MINT-634438.
STRINGi 4932.YGL195W.

Proteomic databases

MaxQBi P33892.
PaxDbi P33892.
PeptideAtlasi P33892.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL195W ; YGL195W ; YGL195W .
GeneIDi 852680.
KEGGi sce:YGL195W.

Organism-specific databases

CYGDi YGL195w.
SGDi S000003163. GCN1.

Phylogenomic databases

eggNOGi NOG325174.
GeneTreei ENSGT00390000011675.
HOGENOMi HOG000213714.
OMAi DEIAWRT.
OrthoDBi EOG7FJH7S.

Enzyme and pathway databases

BioCyci YEAST:G3O-30676-MONOMER.

Miscellaneous databases

NextBioi 971993.
PROi P33892.

Gene expression databases

Genevestigatori P33892.

Family and domain databases

Gene3Di 1.25.10.10. 8 hits.
InterProi IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR022716. DUF3554.
IPR026827. ECM29/GCN1.
IPR021133. HEAT_type_2.
[Graphical view ]
PANTHERi PTHR23346. PTHR23346. 1 hit.
Pfami PF12074. DUF3554. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 8 hits.
PROSITEi PS50077. HEAT_REPEAT. 4 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "GCN1, a translational activator of GCN4 in Saccharomyces cerevisiae, is required for phosphorylation of eukaryotic translation initiation factor 2 by protein kinase GCN2."
    Marton M.J., Crouch D., Hinnebusch A.G.
    Mol. Cell. Biol. 13:3541-3556(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae."
    Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V.
    Yeast 13:55-64(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "YIH1 is an actin-binding protein that inhibits protein kinase GCN2 and impairs general amino acid control when overexpressed."
    Sattlegger E., Swanson M.J., Ashcraft E.A., Jennings J.L., Fekete R.A., Link A.J., Hinnebusch A.G.
    J. Biol. Chem. 279:29952-29962(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH YIH1.
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "Saccharomyces cerevisiae Rbg1 protein and its binding partner Gir2 interact on polyribosomes with Gcn1."
    Wout P.K., Sattlegger E., Sullivan S.M., Maddock J.R.
    Eukaryot. Cell 8:1061-1071(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GIR2.
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGCN1_YEAST
AccessioniPrimary (citable) accession number: P33892
Secondary accession number(s): D6VTV8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: September 3, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7330 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

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