P33892 (GCN1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Translational activator GCN1

Gene names

Name:	GCN1
Ordered Locus Names:	YGL195W
ORF Names:	G1318

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	2672 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a translation activator that mediates translational control by regulating GCN2 kinase activity. Translational activator of GCN4. May be involved in sensing charged tRNA and stimulating the kinase activity of GCN2 in amino acid-starved cells. Required in vivo for the phosphorylation of eIF-2-alpha on 'Ser-52' by the protein kinase GCN2.
Subunit structure	Component of a heteromeric complex that includes GCN1 and GCN20. Interacts with YIH1; preventing the interaction with GCN2 protein kinase. Interacts with GIR2. Ref.6 Ref.7
Miscellaneous	Present with 7330 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the GCN1 family. Contains 20 HEAT repeats.

Ontologies

Keywords
Biological process	Translation regulation
Domain	Repeat
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of translational elongation Inferred from physical interaction PubMed 9234705. Source: SGD
Cellular_component	cytosolic ribosome Inferred from direct assay PubMed 9234705. Source: SGD mitochondrion Inferred from direct assay PubMed 14576278PubMed 16823961. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GCN20	P43535	4	EBI-7442,EBI-7423

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2672	2672	Translational activator GCN1		PRO_0000087444
Regions
	Repeat	932 – 970	39	HEAT 1
	Repeat	1030 – 1067	38	HEAT 2
	Repeat	1099 – 1138	40	HEAT 3
	Repeat	1243 – 1281	39	HEAT 4
	Repeat	1284 – 1321	38	HEAT 5
	Repeat	1405 – 1442	38	HEAT 6
	Repeat	1484 – 1521	38	HEAT 7
	Repeat	1523 – 1559	37	HEAT 8
	Repeat	1561 – 1598	38	HEAT 9
	Repeat	1603 – 1640	38	HEAT 10
	Repeat	1641 – 1679	39	HEAT 11
	Repeat	1721 – 1758	38	HEAT 12
	Repeat	1760 – 1796	37	HEAT 13
	Repeat	1862 – 1903	42	HEAT 14
	Repeat	1905 – 1942	38	HEAT 15
	Repeat	1947 – 1984	38	HEAT 16
	Repeat	1985 – 2024	40	HEAT 17
	Repeat	2097 – 2134	38	HEAT 18
	Repeat	2290 – 2328	39	HEAT 19
	Repeat	2347 – 2384	38	HEAT 20

Sequences

	Sequence		Length	Mass (Da)
	P33892 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 980FDD03753E9D1C	FASTA	2,672	296,698
	10 20 30 40 50 60 MTAILNWEDI SPVLEKGTRE SHVSKRVPFL QDISQLVRQE TLEKPQLSEI AFVLLNTFTI 70 80 90 100 110 120 YEDNRSKSLV TSILLDILNL EPCLLENFIR FISDVVISNP ATKAVADYLN LLDWINSFLI 130 140 150 160 170 180 FVSHNSNLFE EYIPKLLVAH SYATFGVETI LDNQEEGKKS QDKQNQHRKR IRYCIFQTTV 190 200 210 220 230 240 KAFLKCLKDN DDSISFMKIS IKTVLESYSK LKITSVGVVM IMGALTQAAL QLLSRQPALH 250 260 270 280 290 300 SVLKENSAEK YCEYLGKEVF LGKNPPSSFC LEIGLKPFLK EFVSQELFIK FFIPNIEKAV 310 320 330 340 350 360 LRSPEVGFSI LSELYAGVSP EKVNLLNAFA SSKLINQYFS SFKSSKEVVR SVSLQSMIIL 370 380 390 400 410 420 LRKISNTDTT LEDLTKLIDE IFKNIKSNLN ADYKSLISKI LIEIPLTHYE VSEKICKGLS 430 440 450 460 470 480 PYIGKEGNEA ALTLMLNAFF VHYFSLGKPI EDLDKIISAG FADKKPALKK CWFAAFLNNS 490 500 510 520 530 540 NAASEEVILN FIDGCLEFVK DSIIHYQTHG HACILASIEF TNKILALDNT ELNDRVMQLI 550 560 570 580 590 600 ETLPENSSIG DAILTAALST ELSIENRIHA VNLLQELFYK KPEFIGFSVI DAIERRMRVQ 610 620 630 640 650 660 ELIPQQNTSF KYVTSVLLAI TSELPDKEAS IKVLINALVI AQWNIFNIKN GWAGLVLRAR 670 680 690 700 710 720 LDPAEVVKEH ASVIMEKILE ITGSCEWIDT IYGACGLQAA AYAAFIQPNE FTPILCKTIE 730 740 750 760 770 780 ADLTADDFSR LSEEDFEIFA GEEGVLVVDV LEESMNKKLS NKNSKEYETL MWEQKIRKEQ 790 800 810 820 830 840 AKKNVKKLSK EEQELVNEQL AKESAVRSHV SEISTRLKRG IRLVSELSKA ACLVQNGIAT 850 860 870 880 890 900 WFPLAVTKLL YLCSEPNISK LTEDVNNVFL QLSQNVSERL GNIRLFLGLA TLRVHNANGI 910 920 930 940 950 960 SQDYLQEPLV ELLTRVLFRI KFVSNQAAID SISLTYILPL LINVLEKGKA IALKNADKPV 970 980 990 1000 1010 1020 VKAEFVEEDE EEEHLLLAME IISVHAEAFE DPSIPRISIV EVLLSLLSLP SKAKIAKDCF 1030 1040 1050 1060 1070 1080 NALCQSISVA PNQEDLDMIL SNLLSPNQFV RSTILETLDN EFELEPFMKY SPEVFICRFD 1090 1100 1110 1120 1130 1140 SDPSNREIAD FIWEFNKFVV NDELLKSLFP LFNQDDSGLR LFAANAYAFG AVSLFTSEEN 1150 1160 1170 1180 1190 1200 SSKDYLNDLL NFYKEKAKPL EPILDQFGLV LVSASEQKDP WQGRSTVAIT LKIMAKAFSA 1210 1220 1230 1240 1250 1260 EDDTVVNIIK FLVDDGGLVD REPIVRQEMK EAGVELITLH GSQNSKDLIP IFEEALSSST 1270 1280 1290 1300 1310 1320 DSALKENVII LYGTLARHLQ QSDARIHTII ERLLSTLDTP SADIQQAVSA CIAPLVFQFK 1330 1340 1350 1360 1370 1380 QKVGDYLGIL MEKLLNPTVA SSMRKGAAWG IAGLVKGYGI SALSEFDIIR NLIEAAEDKK 1390 1400 1410 1420 1430 1440 EPKRRESVGF CFQYLSESLG KFFEPYVIEI LPNILKNLGD AVPEVRDATA RATKAIMAHT 1450 1460 1470 1480 1490 1500 TGYGVKKLIP VAVSNLDEIA WRTKRGSVQL LGNMAYLDPT QLSASLSTIV PEIVGVLNDS 1510 1520 1530 1540 1550 1560 HKEVRKAADE SLKRFGEVIR NPEIQKLVPV LLQAIGDPTK YTEEALDSLI QTQFVHYIDG 1570 1580 1590 1600 1610 1620 PSLALIIHII HRGMHDRSAN IKRKACKIVG NMAILVDTKD LIPYLQQLID EVEIAMVDPV 1630 1640 1650 1660 1670 1680 PNTRATAARA LGALVERLGE EQFPDLIPRL LDTLSDESKS GDRLGSAQAL AEVISGLGLT 1690 1700 1710 1720 1730 1740 KLDEMLPTIL AGVTNFRAYI REGFMPLLLF LPVCFGSQFA PYINQIIQPI LSGLADNDEN 1750 1760 1770 1780 1790 1800 IRDTALKAGK LIVKNYATKA VDLLLPELER GMFDENDRIR LSSVQLTGEL LFQVTGISSR 1810 1820 1830 1840 1850 1860 NEFSEEDGDH NGEFSGKLVD VLGQDRRDRI LAALFVCRND TSGIVRATTV DIWKALVPNT 1870 1880 1890 1900 1910 1920 PRAVKEILPT LTGMIVTHLA SSSNVLRNIA AQTLGDLVRR VGGNALSQLL PSLEESLIET 1930 1940 1950 1960 1970 1980 SNSDSRQGVC IALYELIESA STETISQFQS TIVNIIRTAL IDESATVREA AALSFDVFQD 1990 2000 2010 2020 2030 2040 VVGKTAVDEV LPYLLHMLES SDNSDFALLG LQEIMSKKSD VIFPILIPTL LAPPIDAFRA 2050 2060 2070 2080 2090 2100 SALGSLAEVA GSALYKRLSI IINALVDAII GTSEDESTKG ALELALDRVF LSVNDDEGLH 2110 2120 2130 2140 2150 2160 PLLQQIMSLL KSDNIEKRIA VLERLPNFFD KTVLDFDVYI PNFVSHAILS LDDEDQRVVN 2170 2180 2190 2200 2210 2220 GNFNALSTLL KKVDKPTLEK LVKPAKQSLA LTGRQGQDVA AFKLPRGPNC VLPIFLHGLM 2230 2240 2250 2260 2270 2280 YGSNDEREES ALAIADVVSK TPAANLKPFV SVITGPLIRV VGERFSSDIK AAILFALNVL 2290 2300 2310 2320 2330 2340 FIKIPMFLRP FIPQLQRTFV KSLSDATNET LRLRAAKALG ALIEHQPRVD PLVIELVTGA 2350 2360 2370 2380 2390 2400 KQATDEGVKT AMLKALLEVI MKAGSKLNEN SKTNIVNLVE EEMLGSNDKL AVAYAKLIGS 2410 2420 2430 2440 2450 2460 LSEILSNDEA HKILQDKVLN ADLDGETGKF AILTLNSFLK DAPTHIFNTG LIDEFVSYIL 2470 2480 2490 2500 2510 2520 NAIRSPDVYF GENGTIAAGK LLLLEGEKRS PFVKKDAAEP FKIGDENINL LINELSKAVL 2530 2540 2550 2560 2570 2580 QPASNSTDVR RLALVVIRTL ARFKFDECIK QYFDVVGPSV FSCLRDPVIP IKLAAEKAYL 2590 2600 2610 2620 2630 2640 ALFKLVEEDD MHTFNEWFAK ISDRGNSIET VTGTTIQLRS VGDYTKRVGK RLANVERERI 2650 2660 2670 AAGGDAETMF SDRFEDEREI WAVGGVELTT DI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"GCN1, a translational activator of GCN4 in Saccharomyces cerevisiae, is required for phosphorylation of eukaryotic translation initiation factor 2 by protein kinase GCN2." Marton M.J., Crouch D., Hinnebusch A.G. Mol. Cell. Biol. 13:3541-3556(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae." Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V. Yeast 13:55-64(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"YIH1 is an actin-binding protein that inhibits protein kinase GCN2 and impairs general amino acid control when overexpressed." Sattlegger E., Swanson M.J., Ashcraft E.A., Jennings J.L., Fekete R.A., Link A.J., Hinnebusch A.G. J. Biol. Chem. 279:29952-29962(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH YIH1.
[7]	"Saccharomyces cerevisiae Rbg1 protein and its binding partner Gir2 interact on polyribosomes with Gcn1." Wout P.K., Sattlegger E., Sullivan S.M., Maddock J.R. Eukaryot. Cell 8:1061-1071(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GIR2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L12467 Genomic DNA. Translation: AAA34635.1. X91837 Genomic DNA. Translation: CAA62949.1. Z72717 Genomic DNA. Translation: CAA96907.1. BK006941 Genomic DNA. Translation: DAA07919.1.
PIR	A48126.
RefSeq	NP_011320.3. NM_001181060.3.
3D structure databases
ProteinModelPortal	P33892.
SMR	P33892. Positions 1404-1674, 2213-2245.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2344N.
IntAct	P33892. 129 interactions.
MINT	MINT-634438.
STRING	4932.YGL195W.
Proteomic databases
PaxDb	P33892.
PeptideAtlas	P33892.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL195W; YGL195W; YGL195W.
GeneID	852680.
KEGG	sce:YGL192W. sce:YGL195W.
Organism-specific databases
CYGD	YGL195w.
SGD	S000003163. GCN1.
Phylogenomic databases
eggNOG	NOG325174.
GeneTree	ENSGT00390000011675.
HOGENOM	HOG000213714.
OMA	KRFGEVI.
OrthoDB	EOG480N4Q.
Gene expression databases
Genevestigator	P33892.
GermOnline	YGL195W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.25.10.10. 8 hits.
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR022716. DUF3554. IPR026827. ECM29/GCN1. IPR021133. HEAT_type_2. [Graphical view]
PANTHER	PTHR23346. PTHR23346. 1 hit.
Pfam	PF12074. DUF3554. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 4 hits.
PROSITE	PS50077. HEAT_REPEAT. 4 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	971993.

Entry information

Entry name

GCN1_YEAST

Accession

Primary (citable) accession number: P33892
Secondary accession number(s): D6VTV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program