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P33892

- GCN1_YEAST

UniProt

P33892 - GCN1_YEAST

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Protein

Translational activator GCN1

Gene

GCN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Acts as a translation activator that mediates translational control by regulating GCN2 kinase activity. Translational activator of GCN4. May be involved in sensing charged tRNA and stimulating the kinase activity of GCN2 in amino acid-starved cells. Required in vivo for the phosphorylation of eIF-2-alpha on 'Ser-52' by the protein kinase GCN2.

GO - Biological processi

  1. regulation of translational elongation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Translation regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-30676-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Translational activator GCN1
Gene namesi
Name:GCN1
Ordered Locus Names:YGL195W
ORF Names:G1318
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL195w.
SGDiS000003163. GCN1.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: SGD
  2. cytosolic ribosome Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 26722672Translational activator GCN1PRO_0000087444Add
BLAST

Proteomic databases

MaxQBiP33892.
PaxDbiP33892.
PeptideAtlasiP33892.

Expressioni

Gene expression databases

GenevestigatoriP33892.

Interactioni

Subunit structurei

Component of a heteromeric complex that includes GCN1 and GCN20. Interacts with YIH1; preventing the interaction with GCN2 protein kinase. Interacts with GIR2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GCN20P435354EBI-7442,EBI-7423

Protein-protein interaction databases

BioGridi33062. 288 interactions.
DIPiDIP-2344N.
IntActiP33892. 128 interactions.
MINTiMINT-634438.
STRINGi4932.YGL195W.

Structurei

3D structure databases

ProteinModelPortaliP33892.
SMRiP33892. Positions 1290-1317, 1404-1674, 1727-1789.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati932 – 97039HEAT 1Add
BLAST
Repeati1030 – 106738HEAT 2Add
BLAST
Repeati1099 – 113840HEAT 3Add
BLAST
Repeati1243 – 128139HEAT 4Add
BLAST
Repeati1284 – 132138HEAT 5Add
BLAST
Repeati1405 – 144238HEAT 6Add
BLAST
Repeati1484 – 152138HEAT 7Add
BLAST
Repeati1523 – 155937HEAT 8Add
BLAST
Repeati1561 – 159838HEAT 9Add
BLAST
Repeati1603 – 164038HEAT 10Add
BLAST
Repeati1641 – 167939HEAT 11Add
BLAST
Repeati1721 – 175838HEAT 12Add
BLAST
Repeati1760 – 179637HEAT 13Add
BLAST
Repeati1862 – 190342HEAT 14Add
BLAST
Repeati1905 – 194238HEAT 15Add
BLAST
Repeati1947 – 198438HEAT 16Add
BLAST
Repeati1985 – 202440HEAT 17Add
BLAST
Repeati2097 – 213438HEAT 18Add
BLAST
Repeati2290 – 232839HEAT 19Add
BLAST
Repeati2347 – 238438HEAT 20Add
BLAST

Sequence similaritiesi

Belongs to the GCN1 family.Curated
Contains 20 HEAT repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG325174.
GeneTreeiENSGT00390000011675.
HOGENOMiHOG000213714.
InParanoidiP33892.
OMAiDEIAWRT.
OrthoDBiEOG7FJH7S.

Family and domain databases

Gene3Di1.25.10.10. 8 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR022716. DUF3554.
IPR026827. ECM29/GCN1.
IPR021133. HEAT_type_2.
[Graphical view]
PANTHERiPTHR23346. PTHR23346. 1 hit.
PfamiPF12074. DUF3554. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 8 hits.
PROSITEiPS50077. HEAT_REPEAT. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33892-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTAILNWEDI SPVLEKGTRE SHVSKRVPFL QDISQLVRQE TLEKPQLSEI
60 70 80 90 100
AFVLLNTFTI YEDNRSKSLV TSILLDILNL EPCLLENFIR FISDVVISNP
110 120 130 140 150
ATKAVADYLN LLDWINSFLI FVSHNSNLFE EYIPKLLVAH SYATFGVETI
160 170 180 190 200
LDNQEEGKKS QDKQNQHRKR IRYCIFQTTV KAFLKCLKDN DDSISFMKIS
210 220 230 240 250
IKTVLESYSK LKITSVGVVM IMGALTQAAL QLLSRQPALH SVLKENSAEK
260 270 280 290 300
YCEYLGKEVF LGKNPPSSFC LEIGLKPFLK EFVSQELFIK FFIPNIEKAV
310 320 330 340 350
LRSPEVGFSI LSELYAGVSP EKVNLLNAFA SSKLINQYFS SFKSSKEVVR
360 370 380 390 400
SVSLQSMIIL LRKISNTDTT LEDLTKLIDE IFKNIKSNLN ADYKSLISKI
410 420 430 440 450
LIEIPLTHYE VSEKICKGLS PYIGKEGNEA ALTLMLNAFF VHYFSLGKPI
460 470 480 490 500
EDLDKIISAG FADKKPALKK CWFAAFLNNS NAASEEVILN FIDGCLEFVK
510 520 530 540 550
DSIIHYQTHG HACILASIEF TNKILALDNT ELNDRVMQLI ETLPENSSIG
560 570 580 590 600
DAILTAALST ELSIENRIHA VNLLQELFYK KPEFIGFSVI DAIERRMRVQ
610 620 630 640 650
ELIPQQNTSF KYVTSVLLAI TSELPDKEAS IKVLINALVI AQWNIFNIKN
660 670 680 690 700
GWAGLVLRAR LDPAEVVKEH ASVIMEKILE ITGSCEWIDT IYGACGLQAA
710 720 730 740 750
AYAAFIQPNE FTPILCKTIE ADLTADDFSR LSEEDFEIFA GEEGVLVVDV
760 770 780 790 800
LEESMNKKLS NKNSKEYETL MWEQKIRKEQ AKKNVKKLSK EEQELVNEQL
810 820 830 840 850
AKESAVRSHV SEISTRLKRG IRLVSELSKA ACLVQNGIAT WFPLAVTKLL
860 870 880 890 900
YLCSEPNISK LTEDVNNVFL QLSQNVSERL GNIRLFLGLA TLRVHNANGI
910 920 930 940 950
SQDYLQEPLV ELLTRVLFRI KFVSNQAAID SISLTYILPL LINVLEKGKA
960 970 980 990 1000
IALKNADKPV VKAEFVEEDE EEEHLLLAME IISVHAEAFE DPSIPRISIV
1010 1020 1030 1040 1050
EVLLSLLSLP SKAKIAKDCF NALCQSISVA PNQEDLDMIL SNLLSPNQFV
1060 1070 1080 1090 1100
RSTILETLDN EFELEPFMKY SPEVFICRFD SDPSNREIAD FIWEFNKFVV
1110 1120 1130 1140 1150
NDELLKSLFP LFNQDDSGLR LFAANAYAFG AVSLFTSEEN SSKDYLNDLL
1160 1170 1180 1190 1200
NFYKEKAKPL EPILDQFGLV LVSASEQKDP WQGRSTVAIT LKIMAKAFSA
1210 1220 1230 1240 1250
EDDTVVNIIK FLVDDGGLVD REPIVRQEMK EAGVELITLH GSQNSKDLIP
1260 1270 1280 1290 1300
IFEEALSSST DSALKENVII LYGTLARHLQ QSDARIHTII ERLLSTLDTP
1310 1320 1330 1340 1350
SADIQQAVSA CIAPLVFQFK QKVGDYLGIL MEKLLNPTVA SSMRKGAAWG
1360 1370 1380 1390 1400
IAGLVKGYGI SALSEFDIIR NLIEAAEDKK EPKRRESVGF CFQYLSESLG
1410 1420 1430 1440 1450
KFFEPYVIEI LPNILKNLGD AVPEVRDATA RATKAIMAHT TGYGVKKLIP
1460 1470 1480 1490 1500
VAVSNLDEIA WRTKRGSVQL LGNMAYLDPT QLSASLSTIV PEIVGVLNDS
1510 1520 1530 1540 1550
HKEVRKAADE SLKRFGEVIR NPEIQKLVPV LLQAIGDPTK YTEEALDSLI
1560 1570 1580 1590 1600
QTQFVHYIDG PSLALIIHII HRGMHDRSAN IKRKACKIVG NMAILVDTKD
1610 1620 1630 1640 1650
LIPYLQQLID EVEIAMVDPV PNTRATAARA LGALVERLGE EQFPDLIPRL
1660 1670 1680 1690 1700
LDTLSDESKS GDRLGSAQAL AEVISGLGLT KLDEMLPTIL AGVTNFRAYI
1710 1720 1730 1740 1750
REGFMPLLLF LPVCFGSQFA PYINQIIQPI LSGLADNDEN IRDTALKAGK
1760 1770 1780 1790 1800
LIVKNYATKA VDLLLPELER GMFDENDRIR LSSVQLTGEL LFQVTGISSR
1810 1820 1830 1840 1850
NEFSEEDGDH NGEFSGKLVD VLGQDRRDRI LAALFVCRND TSGIVRATTV
1860 1870 1880 1890 1900
DIWKALVPNT PRAVKEILPT LTGMIVTHLA SSSNVLRNIA AQTLGDLVRR
1910 1920 1930 1940 1950
VGGNALSQLL PSLEESLIET SNSDSRQGVC IALYELIESA STETISQFQS
1960 1970 1980 1990 2000
TIVNIIRTAL IDESATVREA AALSFDVFQD VVGKTAVDEV LPYLLHMLES
2010 2020 2030 2040 2050
SDNSDFALLG LQEIMSKKSD VIFPILIPTL LAPPIDAFRA SALGSLAEVA
2060 2070 2080 2090 2100
GSALYKRLSI IINALVDAII GTSEDESTKG ALELALDRVF LSVNDDEGLH
2110 2120 2130 2140 2150
PLLQQIMSLL KSDNIEKRIA VLERLPNFFD KTVLDFDVYI PNFVSHAILS
2160 2170 2180 2190 2200
LDDEDQRVVN GNFNALSTLL KKVDKPTLEK LVKPAKQSLA LTGRQGQDVA
2210 2220 2230 2240 2250
AFKLPRGPNC VLPIFLHGLM YGSNDEREES ALAIADVVSK TPAANLKPFV
2260 2270 2280 2290 2300
SVITGPLIRV VGERFSSDIK AAILFALNVL FIKIPMFLRP FIPQLQRTFV
2310 2320 2330 2340 2350
KSLSDATNET LRLRAAKALG ALIEHQPRVD PLVIELVTGA KQATDEGVKT
2360 2370 2380 2390 2400
AMLKALLEVI MKAGSKLNEN SKTNIVNLVE EEMLGSNDKL AVAYAKLIGS
2410 2420 2430 2440 2450
LSEILSNDEA HKILQDKVLN ADLDGETGKF AILTLNSFLK DAPTHIFNTG
2460 2470 2480 2490 2500
LIDEFVSYIL NAIRSPDVYF GENGTIAAGK LLLLEGEKRS PFVKKDAAEP
2510 2520 2530 2540 2550
FKIGDENINL LINELSKAVL QPASNSTDVR RLALVVIRTL ARFKFDECIK
2560 2570 2580 2590 2600
QYFDVVGPSV FSCLRDPVIP IKLAAEKAYL ALFKLVEEDD MHTFNEWFAK
2610 2620 2630 2640 2650
ISDRGNSIET VTGTTIQLRS VGDYTKRVGK RLANVERERI AAGGDAETMF
2660 2670
SDRFEDEREI WAVGGVELTT DI
Length:2,672
Mass (Da):296,698
Last modified:February 1, 1994 - v1
Checksum:i980FDD03753E9D1C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12467 Genomic DNA. Translation: AAA34635.1.
X91837 Genomic DNA. Translation: CAA62949.1.
Z72717 Genomic DNA. Translation: CAA96907.1.
BK006941 Genomic DNA. Translation: DAA07919.1.
PIRiA48126.
RefSeqiNP_011320.3. NM_001181060.3.

Genome annotation databases

EnsemblFungiiYGL195W; YGL195W; YGL195W.
GeneIDi852680.
KEGGisce:YGL195W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12467 Genomic DNA. Translation: AAA34635.1 .
X91837 Genomic DNA. Translation: CAA62949.1 .
Z72717 Genomic DNA. Translation: CAA96907.1 .
BK006941 Genomic DNA. Translation: DAA07919.1 .
PIRi A48126.
RefSeqi NP_011320.3. NM_001181060.3.

3D structure databases

ProteinModelPortali P33892.
SMRi P33892. Positions 1290-1317, 1404-1674, 1727-1789.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33062. 288 interactions.
DIPi DIP-2344N.
IntActi P33892. 128 interactions.
MINTi MINT-634438.
STRINGi 4932.YGL195W.

Proteomic databases

MaxQBi P33892.
PaxDbi P33892.
PeptideAtlasi P33892.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL195W ; YGL195W ; YGL195W .
GeneIDi 852680.
KEGGi sce:YGL195W.

Organism-specific databases

CYGDi YGL195w.
SGDi S000003163. GCN1.

Phylogenomic databases

eggNOGi NOG325174.
GeneTreei ENSGT00390000011675.
HOGENOMi HOG000213714.
InParanoidi P33892.
OMAi DEIAWRT.
OrthoDBi EOG7FJH7S.

Enzyme and pathway databases

BioCyci YEAST:G3O-30676-MONOMER.

Miscellaneous databases

NextBioi 971993.
PROi P33892.

Gene expression databases

Genevestigatori P33892.

Family and domain databases

Gene3Di 1.25.10.10. 8 hits.
InterProi IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR022716. DUF3554.
IPR026827. ECM29/GCN1.
IPR021133. HEAT_type_2.
[Graphical view ]
PANTHERi PTHR23346. PTHR23346. 1 hit.
Pfami PF12074. DUF3554. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 8 hits.
PROSITEi PS50077. HEAT_REPEAT. 4 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "GCN1, a translational activator of GCN4 in Saccharomyces cerevisiae, is required for phosphorylation of eukaryotic translation initiation factor 2 by protein kinase GCN2."
    Marton M.J., Crouch D., Hinnebusch A.G.
    Mol. Cell. Biol. 13:3541-3556(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae."
    Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V.
    Yeast 13:55-64(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "YIH1 is an actin-binding protein that inhibits protein kinase GCN2 and impairs general amino acid control when overexpressed."
    Sattlegger E., Swanson M.J., Ashcraft E.A., Jennings J.L., Fekete R.A., Link A.J., Hinnebusch A.G.
    J. Biol. Chem. 279:29952-29962(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH YIH1.
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "Saccharomyces cerevisiae Rbg1 protein and its binding partner Gir2 interact on polyribosomes with Gcn1."
    Wout P.K., Sattlegger E., Sullivan S.M., Maddock J.R.
    Eukaryot. Cell 8:1061-1071(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GIR2.
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGCN1_YEAST
AccessioniPrimary (citable) accession number: P33892
Secondary accession number(s): D6VTV8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 26, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7330 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3