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P33892

- GCN1_YEAST

UniProt

P33892 - GCN1_YEAST

Protein

Translational activator GCN1

Gene

GCN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Acts as a translation activator that mediates translational control by regulating GCN2 kinase activity. Translational activator of GCN4. May be involved in sensing charged tRNA and stimulating the kinase activity of GCN2 in amino acid-starved cells. Required in vivo for the phosphorylation of eIF-2-alpha on 'Ser-52' by the protein kinase GCN2.

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. regulation of translational elongation Source: SGD

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Translation regulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30676-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Translational activator GCN1
    Gene namesi
    Name:GCN1
    Ordered Locus Names:YGL195W
    ORF Names:G1318
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL195w.
    SGDiS000003163. GCN1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. cytosolic ribosome Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 26722672Translational activator GCN1PRO_0000087444Add
    BLAST

    Proteomic databases

    MaxQBiP33892.
    PaxDbiP33892.
    PeptideAtlasiP33892.

    Expressioni

    Gene expression databases

    GenevestigatoriP33892.

    Interactioni

    Subunit structurei

    Component of a heteromeric complex that includes GCN1 and GCN20. Interacts with YIH1; preventing the interaction with GCN2 protein kinase. Interacts with GIR2.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GCN20P435354EBI-7442,EBI-7423

    Protein-protein interaction databases

    BioGridi33062. 288 interactions.
    DIPiDIP-2344N.
    IntActiP33892. 128 interactions.
    MINTiMINT-634438.
    STRINGi4932.YGL195W.

    Structurei

    3D structure databases

    ProteinModelPortaliP33892.
    SMRiP33892. Positions 1404-1674.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati932 – 97039HEAT 1Add
    BLAST
    Repeati1030 – 106738HEAT 2Add
    BLAST
    Repeati1099 – 113840HEAT 3Add
    BLAST
    Repeati1243 – 128139HEAT 4Add
    BLAST
    Repeati1284 – 132138HEAT 5Add
    BLAST
    Repeati1405 – 144238HEAT 6Add
    BLAST
    Repeati1484 – 152138HEAT 7Add
    BLAST
    Repeati1523 – 155937HEAT 8Add
    BLAST
    Repeati1561 – 159838HEAT 9Add
    BLAST
    Repeati1603 – 164038HEAT 10Add
    BLAST
    Repeati1641 – 167939HEAT 11Add
    BLAST
    Repeati1721 – 175838HEAT 12Add
    BLAST
    Repeati1760 – 179637HEAT 13Add
    BLAST
    Repeati1862 – 190342HEAT 14Add
    BLAST
    Repeati1905 – 194238HEAT 15Add
    BLAST
    Repeati1947 – 198438HEAT 16Add
    BLAST
    Repeati1985 – 202440HEAT 17Add
    BLAST
    Repeati2097 – 213438HEAT 18Add
    BLAST
    Repeati2290 – 232839HEAT 19Add
    BLAST
    Repeati2347 – 238438HEAT 20Add
    BLAST

    Sequence similaritiesi

    Belongs to the GCN1 family.Curated
    Contains 20 HEAT repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG325174.
    GeneTreeiENSGT00390000011675.
    HOGENOMiHOG000213714.
    OMAiDEIAWRT.
    OrthoDBiEOG7FJH7S.

    Family and domain databases

    Gene3Di1.25.10.10. 8 hits.
    InterProiIPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR022716. DUF3554.
    IPR026827. ECM29/GCN1.
    IPR021133. HEAT_type_2.
    [Graphical view]
    PANTHERiPTHR23346. PTHR23346. 1 hit.
    PfamiPF12074. DUF3554. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 8 hits.
    PROSITEiPS50077. HEAT_REPEAT. 4 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P33892-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTAILNWEDI SPVLEKGTRE SHVSKRVPFL QDISQLVRQE TLEKPQLSEI     50
    AFVLLNTFTI YEDNRSKSLV TSILLDILNL EPCLLENFIR FISDVVISNP 100
    ATKAVADYLN LLDWINSFLI FVSHNSNLFE EYIPKLLVAH SYATFGVETI 150
    LDNQEEGKKS QDKQNQHRKR IRYCIFQTTV KAFLKCLKDN DDSISFMKIS 200
    IKTVLESYSK LKITSVGVVM IMGALTQAAL QLLSRQPALH SVLKENSAEK 250
    YCEYLGKEVF LGKNPPSSFC LEIGLKPFLK EFVSQELFIK FFIPNIEKAV 300
    LRSPEVGFSI LSELYAGVSP EKVNLLNAFA SSKLINQYFS SFKSSKEVVR 350
    SVSLQSMIIL LRKISNTDTT LEDLTKLIDE IFKNIKSNLN ADYKSLISKI 400
    LIEIPLTHYE VSEKICKGLS PYIGKEGNEA ALTLMLNAFF VHYFSLGKPI 450
    EDLDKIISAG FADKKPALKK CWFAAFLNNS NAASEEVILN FIDGCLEFVK 500
    DSIIHYQTHG HACILASIEF TNKILALDNT ELNDRVMQLI ETLPENSSIG 550
    DAILTAALST ELSIENRIHA VNLLQELFYK KPEFIGFSVI DAIERRMRVQ 600
    ELIPQQNTSF KYVTSVLLAI TSELPDKEAS IKVLINALVI AQWNIFNIKN 650
    GWAGLVLRAR LDPAEVVKEH ASVIMEKILE ITGSCEWIDT IYGACGLQAA 700
    AYAAFIQPNE FTPILCKTIE ADLTADDFSR LSEEDFEIFA GEEGVLVVDV 750
    LEESMNKKLS NKNSKEYETL MWEQKIRKEQ AKKNVKKLSK EEQELVNEQL 800
    AKESAVRSHV SEISTRLKRG IRLVSELSKA ACLVQNGIAT WFPLAVTKLL 850
    YLCSEPNISK LTEDVNNVFL QLSQNVSERL GNIRLFLGLA TLRVHNANGI 900
    SQDYLQEPLV ELLTRVLFRI KFVSNQAAID SISLTYILPL LINVLEKGKA 950
    IALKNADKPV VKAEFVEEDE EEEHLLLAME IISVHAEAFE DPSIPRISIV 1000
    EVLLSLLSLP SKAKIAKDCF NALCQSISVA PNQEDLDMIL SNLLSPNQFV 1050
    RSTILETLDN EFELEPFMKY SPEVFICRFD SDPSNREIAD FIWEFNKFVV 1100
    NDELLKSLFP LFNQDDSGLR LFAANAYAFG AVSLFTSEEN SSKDYLNDLL 1150
    NFYKEKAKPL EPILDQFGLV LVSASEQKDP WQGRSTVAIT LKIMAKAFSA 1200
    EDDTVVNIIK FLVDDGGLVD REPIVRQEMK EAGVELITLH GSQNSKDLIP 1250
    IFEEALSSST DSALKENVII LYGTLARHLQ QSDARIHTII ERLLSTLDTP 1300
    SADIQQAVSA CIAPLVFQFK QKVGDYLGIL MEKLLNPTVA SSMRKGAAWG 1350
    IAGLVKGYGI SALSEFDIIR NLIEAAEDKK EPKRRESVGF CFQYLSESLG 1400
    KFFEPYVIEI LPNILKNLGD AVPEVRDATA RATKAIMAHT TGYGVKKLIP 1450
    VAVSNLDEIA WRTKRGSVQL LGNMAYLDPT QLSASLSTIV PEIVGVLNDS 1500
    HKEVRKAADE SLKRFGEVIR NPEIQKLVPV LLQAIGDPTK YTEEALDSLI 1550
    QTQFVHYIDG PSLALIIHII HRGMHDRSAN IKRKACKIVG NMAILVDTKD 1600
    LIPYLQQLID EVEIAMVDPV PNTRATAARA LGALVERLGE EQFPDLIPRL 1650
    LDTLSDESKS GDRLGSAQAL AEVISGLGLT KLDEMLPTIL AGVTNFRAYI 1700
    REGFMPLLLF LPVCFGSQFA PYINQIIQPI LSGLADNDEN IRDTALKAGK 1750
    LIVKNYATKA VDLLLPELER GMFDENDRIR LSSVQLTGEL LFQVTGISSR 1800
    NEFSEEDGDH NGEFSGKLVD VLGQDRRDRI LAALFVCRND TSGIVRATTV 1850
    DIWKALVPNT PRAVKEILPT LTGMIVTHLA SSSNVLRNIA AQTLGDLVRR 1900
    VGGNALSQLL PSLEESLIET SNSDSRQGVC IALYELIESA STETISQFQS 1950
    TIVNIIRTAL IDESATVREA AALSFDVFQD VVGKTAVDEV LPYLLHMLES 2000
    SDNSDFALLG LQEIMSKKSD VIFPILIPTL LAPPIDAFRA SALGSLAEVA 2050
    GSALYKRLSI IINALVDAII GTSEDESTKG ALELALDRVF LSVNDDEGLH 2100
    PLLQQIMSLL KSDNIEKRIA VLERLPNFFD KTVLDFDVYI PNFVSHAILS 2150
    LDDEDQRVVN GNFNALSTLL KKVDKPTLEK LVKPAKQSLA LTGRQGQDVA 2200
    AFKLPRGPNC VLPIFLHGLM YGSNDEREES ALAIADVVSK TPAANLKPFV 2250
    SVITGPLIRV VGERFSSDIK AAILFALNVL FIKIPMFLRP FIPQLQRTFV 2300
    KSLSDATNET LRLRAAKALG ALIEHQPRVD PLVIELVTGA KQATDEGVKT 2350
    AMLKALLEVI MKAGSKLNEN SKTNIVNLVE EEMLGSNDKL AVAYAKLIGS 2400
    LSEILSNDEA HKILQDKVLN ADLDGETGKF AILTLNSFLK DAPTHIFNTG 2450
    LIDEFVSYIL NAIRSPDVYF GENGTIAAGK LLLLEGEKRS PFVKKDAAEP 2500
    FKIGDENINL LINELSKAVL QPASNSTDVR RLALVVIRTL ARFKFDECIK 2550
    QYFDVVGPSV FSCLRDPVIP IKLAAEKAYL ALFKLVEEDD MHTFNEWFAK 2600
    ISDRGNSIET VTGTTIQLRS VGDYTKRVGK RLANVERERI AAGGDAETMF 2650
    SDRFEDEREI WAVGGVELTT DI 2672
    Length:2,672
    Mass (Da):296,698
    Last modified:February 1, 1994 - v1
    Checksum:i980FDD03753E9D1C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L12467 Genomic DNA. Translation: AAA34635.1.
    X91837 Genomic DNA. Translation: CAA62949.1.
    Z72717 Genomic DNA. Translation: CAA96907.1.
    BK006941 Genomic DNA. Translation: DAA07919.1.
    PIRiA48126.
    RefSeqiNP_011320.3. NM_001181060.3.

    Genome annotation databases

    EnsemblFungiiYGL195W; YGL195W; YGL195W.
    GeneIDi852680.
    KEGGisce:YGL195W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L12467 Genomic DNA. Translation: AAA34635.1 .
    X91837 Genomic DNA. Translation: CAA62949.1 .
    Z72717 Genomic DNA. Translation: CAA96907.1 .
    BK006941 Genomic DNA. Translation: DAA07919.1 .
    PIRi A48126.
    RefSeqi NP_011320.3. NM_001181060.3.

    3D structure databases

    ProteinModelPortali P33892.
    SMRi P33892. Positions 1404-1674.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33062. 288 interactions.
    DIPi DIP-2344N.
    IntActi P33892. 128 interactions.
    MINTi MINT-634438.
    STRINGi 4932.YGL195W.

    Proteomic databases

    MaxQBi P33892.
    PaxDbi P33892.
    PeptideAtlasi P33892.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL195W ; YGL195W ; YGL195W .
    GeneIDi 852680.
    KEGGi sce:YGL195W.

    Organism-specific databases

    CYGDi YGL195w.
    SGDi S000003163. GCN1.

    Phylogenomic databases

    eggNOGi NOG325174.
    GeneTreei ENSGT00390000011675.
    HOGENOMi HOG000213714.
    OMAi DEIAWRT.
    OrthoDBi EOG7FJH7S.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30676-MONOMER.

    Miscellaneous databases

    NextBioi 971993.
    PROi P33892.

    Gene expression databases

    Genevestigatori P33892.

    Family and domain databases

    Gene3Di 1.25.10.10. 8 hits.
    InterProi IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR022716. DUF3554.
    IPR026827. ECM29/GCN1.
    IPR021133. HEAT_type_2.
    [Graphical view ]
    PANTHERi PTHR23346. PTHR23346. 1 hit.
    Pfami PF12074. DUF3554. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 8 hits.
    PROSITEi PS50077. HEAT_REPEAT. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "GCN1, a translational activator of GCN4 in Saccharomyces cerevisiae, is required for phosphorylation of eukaryotic translation initiation factor 2 by protein kinase GCN2."
      Marton M.J., Crouch D., Hinnebusch A.G.
      Mol. Cell. Biol. 13:3541-3556(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae."
      Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V.
      Yeast 13:55-64(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "YIH1 is an actin-binding protein that inhibits protein kinase GCN2 and impairs general amino acid control when overexpressed."
      Sattlegger E., Swanson M.J., Ashcraft E.A., Jennings J.L., Fekete R.A., Link A.J., Hinnebusch A.G.
      J. Biol. Chem. 279:29952-29962(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH YIH1.
    7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    8. "Saccharomyces cerevisiae Rbg1 protein and its binding partner Gir2 interact on polyribosomes with Gcn1."
      Wout P.K., Sattlegger E., Sullivan S.M., Maddock J.R.
      Eukaryot. Cell 8:1061-1071(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GIR2.
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGCN1_YEAST
    AccessioniPrimary (citable) accession number: P33892
    Secondary accession number(s): D6VTV8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 7330 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3