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P33891

- TIP20_YEAST

UniProt

P33891 - TIP20_YEAST

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Protein

Protein transport protein TIP20

Gene
TIP20, TIP1, YGL145W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Required for protein transport between the Golgi and the endoplasmic reticulum. May contribute to tethering of coatomer-coated retrograde transport vesicles to the ER membrane through interaction with and stabilization of the SNARE complex.2 Publications

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. protein transport Source: UniProtKB-KW
  2. retrograde vesicle-mediated transport, Golgi to ER Source: SGD
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30639-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein transport protein TIP20
Gene namesi
Name:TIP20
Synonyms:TIP1
Ordered Locus Names:YGL145W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL145w.
SGDiS000003113. TIP20.

Subcellular locationi

Endoplasmic reticulum membrane; Peripheral membrane protein 1 Publication

GO - Cellular componenti

  1. Dsl1p complex Source: SGD
  2. endoplasmic reticulum Source: SGD
  3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 701701Protein transport protein TIP20PRO_0000072543Add
BLAST

Proteomic databases

MaxQBiP33891.
PaxDbiP33891.

Expressioni

Gene expression databases

GenevestigatoriP33891.

Interactioni

Subunit structurei

Component of a peripheral membrane protein complex consisting of DSL1, SEC39/DSL3 and TIP20. Bound to a SNARE complex consisting of UFE1, USE1, SEC20 and SEC22 or YKT6 through direct interaction of TIP20 with SEC20. Interacts with DSL1, SEC39/DSL3 and the cytoplasmic domain of SEC20.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DSL1P538477EBI-19396,EBI-29249
SEC20P287916EBI-19396,EBI-16572
SEC39Q127454EBI-19396,EBI-31898

Protein-protein interaction databases

BioGridi33107. 27 interactions.
DIPiDIP-2004N.
IntActiP33891. 11 interactions.
MINTiMINT-403248.
STRINGi4932.YGL145W.

Structurei

Secondary structure

1
701
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 3122
Helixi46 – 549
Helixi59 – 679
Helixi73 – 10533
Helixi110 – 1123
Helixi115 – 12915
Helixi142 – 15918
Helixi161 – 17818
Turni179 – 1824
Helixi188 – 1903
Helixi192 – 20817
Helixi239 – 25517
Helixi262 – 28726
Helixi290 – 2923
Helixi296 – 30712
Helixi309 – 32012
Helixi324 – 34421
Helixi352 – 3554
Helixi358 – 37821
Helixi383 – 3886
Helixi389 – 40416
Helixi406 – 4094
Helixi413 – 4164
Helixi417 – 4259
Helixi427 – 43913
Beta strandi446 – 4494
Helixi450 – 47425
Helixi478 – 49013
Helixi500 – 51112
Helixi513 – 52917
Helixi531 – 5355
Helixi538 – 5403
Helixi558 – 5603
Helixi561 – 57515
Helixi581 – 60121
Turni602 – 6065
Helixi611 – 62515
Helixi637 – 64913
Helixi650 – 6534
Helixi655 – 6573
Turni658 – 6614
Helixi663 – 6686
Helixi673 – 6786
Beta strandi681 – 6833
Helixi685 – 69612

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ETVX-ray1.94A1-40[»]
3FHNX-ray3.00A/B/C/D2-701[»]
ProteinModelPortaliP33891.
SMRiP33891. Positions 5-701.

Miscellaneous databases

EvolutionaryTraceiP33891.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini147 – 701555RINT1/TIP20Add
BLAST

Sequence similaritiesi

Contains 1 RINT1/TIP20 domain.

Phylogenomic databases

eggNOGiNOG328678.
HOGENOMiHOG000000922.
OMAiWEKWINY.
OrthoDBiEOG747PSJ.

Family and domain databases

InterProiIPR007528. RINT1_TIP1.
[Graphical view]
PfamiPF04437. RINT1_TIP1. 1 hit.
[Graphical view]
PROSITEiPS51386. RINT1_TIP20. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33891-1 [UniParc]FASTAAdd to Basket

« Hide

MNGIDDLLNI NDRIKQVQNE RNELASKLQN LKQSLASNDT EVALSEVIAQ    50
DIIEVGASVE GLEQLRAKYG DLQILNKLEK VAVQQTQMQA GVDKLDSFER 100
QLDELAEQPP DQFTLDDVKA LHSKLTSVFA TVPQINNIDS QYAAYNKLKS 150
KVTGKYNDVI IQRLATNWSN TFDQKLLEAQ WDTQKFASTS VGLVKCLREN 200
STKLYQLSLL YLPLEEETQN GDSERPLSRS NNNQEPVLWN FKSLANNFNV 250
RFTYHFHATS SSSKIETYFQ FLNDYLAENL YKCINIFHDD CNGLTKPVIH 300
EQFINYVLQP IRDKVRSTLF QNDLKTLIVL ISQILATDKN LLNSFHYHGL 350
GLVSLISDEV WEKWINYEVE MANRQFINIT KNPEDFPKSS QNFVKLINKI 400
YDYLEPFYDL DFDLLVRYKL MTCSLIFMNL TSSYLDYILT VDSLNETRTK 450
EQELYQTMAK LQHVNFVYRK IKSLSSNFIF IQLTDIVNST ESKKYNSLFQ 500
NVENDYEKAM STDMQNSIVH RIQKLLKETL RNYFKISTWS TLEMSVDENI 550
GPSSVPSAEL VNSINVLRRL INKLDSMDIP LAISLKVKNE LLNVIVNYFT 600
ESILKLNKFN QNGLNQFLHD FKSLSSILSL PSHATNYKCM SLHELVKILK 650
LKYDPNNQQF LNPEYIKTGN FTSLKEAYSI KYLKDTKIQD ALYRIIYGNI 700
L 701
Length:701
Mass (Da):81,167
Last modified:February 1, 1994 - v1
Checksum:i3E561DD4C0B9C176
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X72699 Genomic DNA. Translation: CAA51249.1.
X99960 Genomic DNA. Translation: CAA68217.1.
Z72667 Genomic DNA. Translation: CAA96857.1.
BK006941 Genomic DNA. Translation: DAA07965.1.
PIRiS35313.
RefSeqiNP_116577.1. NM_001181010.2.

Genome annotation databases

EnsemblFungiiYGL145W; YGL145W; YGL145W.
GeneIDi852732.
KEGGisce:YGL145W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X72699 Genomic DNA. Translation: CAA51249.1 .
X99960 Genomic DNA. Translation: CAA68217.1 .
Z72667 Genomic DNA. Translation: CAA96857.1 .
BK006941 Genomic DNA. Translation: DAA07965.1 .
PIRi S35313.
RefSeqi NP_116577.1. NM_001181010.2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3ETV X-ray 1.94 A 1-40 [» ]
3FHN X-ray 3.00 A/B/C/D 2-701 [» ]
ProteinModelPortali P33891.
SMRi P33891. Positions 5-701.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33107. 27 interactions.
DIPi DIP-2004N.
IntActi P33891. 11 interactions.
MINTi MINT-403248.
STRINGi 4932.YGL145W.

Proteomic databases

MaxQBi P33891.
PaxDbi P33891.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL145W ; YGL145W ; YGL145W .
GeneIDi 852732.
KEGGi sce:YGL145W.

Organism-specific databases

CYGDi YGL145w.
SGDi S000003113. TIP20.

Phylogenomic databases

eggNOGi NOG328678.
HOGENOMi HOG000000922.
OMAi WEKWINY.
OrthoDBi EOG747PSJ.

Enzyme and pathway databases

BioCyci YEAST:G3O-30639-MONOMER.

Miscellaneous databases

EvolutionaryTracei P33891.
NextBioi 972129.

Gene expression databases

Genevestigatori P33891.

Family and domain databases

InterProi IPR007528. RINT1_TIP1.
[Graphical view ]
Pfami PF04437. RINT1_TIP1. 1 hit.
[Graphical view ]
PROSITEi PS51386. RINT1_TIP20. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The TIP1 gene of Saccharomyces cerevisiae encodes an 80 kDa cytoplasmic protein that interacts with the cytoplasmic domain of Sec20p."
    Sweet D.J., Pelham H.R.B.
    EMBO J. 12:2831-2840(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SEC20.
  2. "The sequence of a nearly unclonable 22.8 kb segment on the left arm chromosome VII from Saccharomyces cerevisiae reveals ARO2, RPL9A, TIP1, MRF1 genes and six new open reading frames."
    Voet M., Defoor E., Verhasselt P., Riles L., Robben J., Volckaert G.
    Yeast 13:177-182(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Isolation of Saccharomyces cerevisiae RNase T1 hypersensitive (rns) mutants and genetic analysis of the RNS1/DSL1 gene."
    Ishikawa T., Unno K., Nonaka G., Nakajima H., Kitamoto K.
    J. Gen. Appl. Microbiol. 51:73-82(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SEC39.
  7. "Dsl1p, Tip20p, and the novel Dsl3(Sec39) protein are required for the stability of the Q/t-SNARE complex at the endoplasmic reticulum in yeast."
    Kraynack B.A., Chan A., Rosenthal E., Essid M., Umansky B., Waters M.G., Schmitt H.D.
    Mol. Biol. Cell 16:3963-3977(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN A COMPLEX WITH DSL1; SEC39; SEC20; USE1 AND UFE1.
  8. Cited for: IDENTIFICATION IN A COMPLEX WITH DSL1; SEC39; SEC20; UFE1; USE1 AND SEC22.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTIP20_YEAST
AccessioniPrimary (citable) accession number: P33891
Secondary accession number(s): D6VU04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: July 9, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6020 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

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