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P33891 (TIP20_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein transport protein TIP20
Gene names
Name:TIP20
Synonyms:TIP1
Ordered Locus Names:YGL145W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length701 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for protein transport between the Golgi and the endoplasmic reticulum. May contribute to tethering of coatomer-coated retrograde transport vesicles to the ER membrane through interaction with and stabilization of the SNARE complex. Ref.1 Ref.7

Subunit structure

Component of a peripheral membrane protein complex consisting of DSL1, SEC39/DSL3 and TIP20. Bound to a SNARE complex consisting of UFE1, USE1, SEC20 and SEC22 or YKT6 through direct interaction of TIP20 with SEC20. Interacts with DSL1, SEC39/DSL3 and the cytoplasmic domain of SEC20. Ref.1 Ref.6 Ref.7 Ref.8

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein Ref.1.

Miscellaneous

Present with 6020 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 RINT1/TIP20 domain.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 701701Protein transport protein TIP20
PRO_0000072543

Regions

Domain147 – 701555RINT1/TIP20

Secondary structure

................................................................................... 701
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P33891 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 3E561DD4C0B9C176

FASTA70181,167
        10         20         30         40         50         60 
MNGIDDLLNI NDRIKQVQNE RNELASKLQN LKQSLASNDT EVALSEVIAQ DIIEVGASVE 

        70         80         90        100        110        120 
GLEQLRAKYG DLQILNKLEK VAVQQTQMQA GVDKLDSFER QLDELAEQPP DQFTLDDVKA 

       130        140        150        160        170        180 
LHSKLTSVFA TVPQINNIDS QYAAYNKLKS KVTGKYNDVI IQRLATNWSN TFDQKLLEAQ 

       190        200        210        220        230        240 
WDTQKFASTS VGLVKCLREN STKLYQLSLL YLPLEEETQN GDSERPLSRS NNNQEPVLWN 

       250        260        270        280        290        300 
FKSLANNFNV RFTYHFHATS SSSKIETYFQ FLNDYLAENL YKCINIFHDD CNGLTKPVIH 

       310        320        330        340        350        360 
EQFINYVLQP IRDKVRSTLF QNDLKTLIVL ISQILATDKN LLNSFHYHGL GLVSLISDEV 

       370        380        390        400        410        420 
WEKWINYEVE MANRQFINIT KNPEDFPKSS QNFVKLINKI YDYLEPFYDL DFDLLVRYKL 

       430        440        450        460        470        480 
MTCSLIFMNL TSSYLDYILT VDSLNETRTK EQELYQTMAK LQHVNFVYRK IKSLSSNFIF 

       490        500        510        520        530        540 
IQLTDIVNST ESKKYNSLFQ NVENDYEKAM STDMQNSIVH RIQKLLKETL RNYFKISTWS 

       550        560        570        580        590        600 
TLEMSVDENI GPSSVPSAEL VNSINVLRRL INKLDSMDIP LAISLKVKNE LLNVIVNYFT 

       610        620        630        640        650        660 
ESILKLNKFN QNGLNQFLHD FKSLSSILSL PSHATNYKCM SLHELVKILK LKYDPNNQQF 

       670        680        690        700 
LNPEYIKTGN FTSLKEAYSI KYLKDTKIQD ALYRIIYGNI L 

« Hide

References

« Hide 'large scale' references
[1]"The TIP1 gene of Saccharomyces cerevisiae encodes an 80 kDa cytoplasmic protein that interacts with the cytoplasmic domain of Sec20p."
Sweet D.J., Pelham H.R.B.
EMBO J. 12:2831-2840(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SEC20.
[2]"The sequence of a nearly unclonable 22.8 kb segment on the left arm chromosome VII from Saccharomyces cerevisiae reveals ARO2, RPL9A, TIP1, MRF1 genes and six new open reading frames."
Voet M., Defoor E., Verhasselt P., Riles L., Robben J., Volckaert G.
Yeast 13:177-182(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Isolation of Saccharomyces cerevisiae RNase T1 hypersensitive (rns) mutants and genetic analysis of the RNS1/DSL1 gene."
Ishikawa T., Unno K., Nonaka G., Nakajima H., Kitamoto K.
J. Gen. Appl. Microbiol. 51:73-82(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SEC39.
[7]"Dsl1p, Tip20p, and the novel Dsl3(Sec39) protein are required for the stability of the Q/t-SNARE complex at the endoplasmic reticulum in yeast."
Kraynack B.A., Chan A., Rosenthal E., Essid M., Umansky B., Waters M.G., Schmitt H.D.
Mol. Biol. Cell 16:3963-3977(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN A COMPLEX WITH DSL1; SEC39; SEC20; USE1 AND UFE1.
[8]"Proteome survey reveals modularity of the yeast cell machinery."
Gavin A.-C., Aloy P., Grandi P., Krause R., Boesche M., Marzioch M., Rau C., Jensen L.J., Bastuck S., Duempelfeld B., Edelmann A., Heurtier M.-A., Hoffman V., Hoefert C., Klein K., Hudak M., Michon A.-M., Schelder M. expand/collapse author list , Schirle M., Remor M., Rudi T., Hooper S., Bauer A., Bouwmeester T., Casari G., Drewes G., Neubauer G., Rick J.M., Kuster B., Bork P., Russell R.B., Superti-Furga G.
Nature 440:631-636(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN A COMPLEX WITH DSL1; SEC39; SEC20; UFE1; USE1 AND SEC22.
[9]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X72699 Genomic DNA. Translation: CAA51249.1.
X99960 Genomic DNA. Translation: CAA68217.1.
Z72667 Genomic DNA. Translation: CAA96857.1.
BK006941 Genomic DNA. Translation: DAA07965.1.
PIRS35313.
RefSeqNP_116577.1. NM_001181010.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3ETVX-ray1.94A1-40[»]
3FHNX-ray3.00A/B/C/D1-701[»]
ProteinModelPortalP33891.
SMRP33891. Positions 5-701.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33107. 27 interactions.
DIPDIP-2004N.
IntActP33891. 11 interactions.
MINTMINT-403248.
STRING4932.YGL145W.

Proteomic databases

PaxDbP33891.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL145W; YGL145W; YGL145W.
GeneID852732.
KEGGsce:YGL145W.

Organism-specific databases

CYGDYGL145w.
SGDS000003113. TIP20.

Phylogenomic databases

eggNOGNOG328678.
HOGENOMHOG000000922.
OMAWEKWINY.
OrthoDBEOG747PSJ.

Enzyme and pathway databases

BioCycYEAST:G3O-30639-MONOMER.

Gene expression databases

GenevestigatorP33891.

Family and domain databases

InterProIPR007528. RINT1_TIP1.
[Graphical view]
PfamPF04437. RINT1_TIP1. 1 hit.
[Graphical view]
PROSITEPS51386. RINT1_TIP20. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP33891.
NextBio972129.

Entry information

Entry nameTIP20_YEAST
AccessionPrimary (citable) accession number: P33891
Secondary accession number(s): D6VU04
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: February 19, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references