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P33891

- TIP20_YEAST

UniProt

P33891 - TIP20_YEAST

Protein

Protein transport protein TIP20

Gene

TIP20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Required for protein transport between the Golgi and the endoplasmic reticulum. May contribute to tethering of coatomer-coated retrograde transport vesicles to the ER membrane through interaction with and stabilization of the SNARE complex.2 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. protein transport Source: UniProtKB-KW
    2. retrograde vesicle-mediated transport, Golgi to ER Source: SGD

    Keywords - Biological processi

    ER-Golgi transport, Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30639-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein transport protein TIP20
    Gene namesi
    Name:TIP20
    Synonyms:TIP1
    Ordered Locus Names:YGL145W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL145w.
    SGDiS000003113. TIP20.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Peripheral membrane protein 1 Publication

    GO - Cellular componenti

    1. Dsl1p complex Source: SGD
    2. endoplasmic reticulum Source: SGD
    3. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 701701Protein transport protein TIP20PRO_0000072543Add
    BLAST

    Proteomic databases

    MaxQBiP33891.
    PaxDbiP33891.

    Expressioni

    Gene expression databases

    GenevestigatoriP33891.

    Interactioni

    Subunit structurei

    Component of a peripheral membrane protein complex consisting of DSL1, SEC39/DSL3 and TIP20. Bound to a SNARE complex consisting of UFE1, USE1, SEC20 and SEC22 or YKT6 through direct interaction of TIP20 with SEC20. Interacts with DSL1, SEC39/DSL3 and the cytoplasmic domain of SEC20.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    DSL1P538477EBI-19396,EBI-29249
    SEC20P287916EBI-19396,EBI-16572
    SEC39Q127454EBI-19396,EBI-31898

    Protein-protein interaction databases

    BioGridi33107. 27 interactions.
    DIPiDIP-2004N.
    IntActiP33891. 11 interactions.
    MINTiMINT-403248.
    STRINGi4932.YGL145W.

    Structurei

    Secondary structure

    1
    701
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi10 – 3122
    Helixi46 – 549
    Helixi59 – 679
    Helixi73 – 10533
    Helixi110 – 1123
    Helixi115 – 12915
    Helixi142 – 15918
    Helixi161 – 17818
    Turni179 – 1824
    Helixi188 – 1903
    Helixi192 – 20817
    Helixi239 – 25517
    Helixi262 – 28726
    Helixi290 – 2923
    Helixi296 – 30712
    Helixi309 – 32012
    Helixi324 – 34421
    Helixi352 – 3554
    Helixi358 – 37821
    Helixi383 – 3886
    Helixi389 – 40416
    Helixi406 – 4094
    Helixi413 – 4164
    Helixi417 – 4259
    Helixi427 – 43913
    Beta strandi446 – 4494
    Helixi450 – 47425
    Helixi478 – 49013
    Helixi500 – 51112
    Helixi513 – 52917
    Helixi531 – 5355
    Helixi538 – 5403
    Helixi558 – 5603
    Helixi561 – 57515
    Helixi581 – 60121
    Turni602 – 6065
    Helixi611 – 62515
    Helixi637 – 64913
    Helixi650 – 6534
    Helixi655 – 6573
    Turni658 – 6614
    Helixi663 – 6686
    Helixi673 – 6786
    Beta strandi681 – 6833
    Helixi685 – 69612

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3ETVX-ray1.94A1-40[»]
    3FHNX-ray3.00A/B/C/D2-701[»]
    ProteinModelPortaliP33891.
    SMRiP33891. Positions 5-701.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP33891.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini147 – 701555RINT1/TIP20PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RINT1/TIP20 domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG328678.
    HOGENOMiHOG000000922.
    OMAiWEKWINY.
    OrthoDBiEOG747PSJ.

    Family and domain databases

    InterProiIPR007528. RINT1_TIP1.
    [Graphical view]
    PfamiPF04437. RINT1_TIP1. 1 hit.
    [Graphical view]
    PROSITEiPS51386. RINT1_TIP20. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P33891-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNGIDDLLNI NDRIKQVQNE RNELASKLQN LKQSLASNDT EVALSEVIAQ    50
    DIIEVGASVE GLEQLRAKYG DLQILNKLEK VAVQQTQMQA GVDKLDSFER 100
    QLDELAEQPP DQFTLDDVKA LHSKLTSVFA TVPQINNIDS QYAAYNKLKS 150
    KVTGKYNDVI IQRLATNWSN TFDQKLLEAQ WDTQKFASTS VGLVKCLREN 200
    STKLYQLSLL YLPLEEETQN GDSERPLSRS NNNQEPVLWN FKSLANNFNV 250
    RFTYHFHATS SSSKIETYFQ FLNDYLAENL YKCINIFHDD CNGLTKPVIH 300
    EQFINYVLQP IRDKVRSTLF QNDLKTLIVL ISQILATDKN LLNSFHYHGL 350
    GLVSLISDEV WEKWINYEVE MANRQFINIT KNPEDFPKSS QNFVKLINKI 400
    YDYLEPFYDL DFDLLVRYKL MTCSLIFMNL TSSYLDYILT VDSLNETRTK 450
    EQELYQTMAK LQHVNFVYRK IKSLSSNFIF IQLTDIVNST ESKKYNSLFQ 500
    NVENDYEKAM STDMQNSIVH RIQKLLKETL RNYFKISTWS TLEMSVDENI 550
    GPSSVPSAEL VNSINVLRRL INKLDSMDIP LAISLKVKNE LLNVIVNYFT 600
    ESILKLNKFN QNGLNQFLHD FKSLSSILSL PSHATNYKCM SLHELVKILK 650
    LKYDPNNQQF LNPEYIKTGN FTSLKEAYSI KYLKDTKIQD ALYRIIYGNI 700
    L 701
    Length:701
    Mass (Da):81,167
    Last modified:February 1, 1994 - v1
    Checksum:i3E561DD4C0B9C176
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X72699 Genomic DNA. Translation: CAA51249.1.
    X99960 Genomic DNA. Translation: CAA68217.1.
    Z72667 Genomic DNA. Translation: CAA96857.1.
    BK006941 Genomic DNA. Translation: DAA07965.1.
    PIRiS35313.
    RefSeqiNP_116577.1. NM_001181010.2.

    Genome annotation databases

    EnsemblFungiiYGL145W; YGL145W; YGL145W.
    GeneIDi852732.
    KEGGisce:YGL145W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X72699 Genomic DNA. Translation: CAA51249.1 .
    X99960 Genomic DNA. Translation: CAA68217.1 .
    Z72667 Genomic DNA. Translation: CAA96857.1 .
    BK006941 Genomic DNA. Translation: DAA07965.1 .
    PIRi S35313.
    RefSeqi NP_116577.1. NM_001181010.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3ETV X-ray 1.94 A 1-40 [» ]
    3FHN X-ray 3.00 A/B/C/D 2-701 [» ]
    ProteinModelPortali P33891.
    SMRi P33891. Positions 5-701.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33107. 27 interactions.
    DIPi DIP-2004N.
    IntActi P33891. 11 interactions.
    MINTi MINT-403248.
    STRINGi 4932.YGL145W.

    Proteomic databases

    MaxQBi P33891.
    PaxDbi P33891.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL145W ; YGL145W ; YGL145W .
    GeneIDi 852732.
    KEGGi sce:YGL145W.

    Organism-specific databases

    CYGDi YGL145w.
    SGDi S000003113. TIP20.

    Phylogenomic databases

    eggNOGi NOG328678.
    HOGENOMi HOG000000922.
    OMAi WEKWINY.
    OrthoDBi EOG747PSJ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30639-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P33891.
    NextBioi 972129.

    Gene expression databases

    Genevestigatori P33891.

    Family and domain databases

    InterProi IPR007528. RINT1_TIP1.
    [Graphical view ]
    Pfami PF04437. RINT1_TIP1. 1 hit.
    [Graphical view ]
    PROSITEi PS51386. RINT1_TIP20. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The TIP1 gene of Saccharomyces cerevisiae encodes an 80 kDa cytoplasmic protein that interacts with the cytoplasmic domain of Sec20p."
      Sweet D.J., Pelham H.R.B.
      EMBO J. 12:2831-2840(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SEC20.
    2. "The sequence of a nearly unclonable 22.8 kb segment on the left arm chromosome VII from Saccharomyces cerevisiae reveals ARO2, RPL9A, TIP1, MRF1 genes and six new open reading frames."
      Voet M., Defoor E., Verhasselt P., Riles L., Robben J., Volckaert G.
      Yeast 13:177-182(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Isolation of Saccharomyces cerevisiae RNase T1 hypersensitive (rns) mutants and genetic analysis of the RNS1/DSL1 gene."
      Ishikawa T., Unno K., Nonaka G., Nakajima H., Kitamoto K.
      J. Gen. Appl. Microbiol. 51:73-82(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SEC39.
    7. "Dsl1p, Tip20p, and the novel Dsl3(Sec39) protein are required for the stability of the Q/t-SNARE complex at the endoplasmic reticulum in yeast."
      Kraynack B.A., Chan A., Rosenthal E., Essid M., Umansky B., Waters M.G., Schmitt H.D.
      Mol. Biol. Cell 16:3963-3977(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN A COMPLEX WITH DSL1; SEC39; SEC20; USE1 AND UFE1.
    8. Cited for: IDENTIFICATION IN A COMPLEX WITH DSL1; SEC39; SEC20; UFE1; USE1 AND SEC22.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTIP20_YEAST
    AccessioniPrimary (citable) accession number: P33891
    Secondary accession number(s): D6VU04
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 6020 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3