Protein transport protein TIP20 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P33891 (TIP20_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 108. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein transport protein TIP20

Gene names

Name:	TIP20
Synonyms:	TIP1
Ordered Locus Names:	YGL145W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	701 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for protein transport between the Golgi and the endoplasmic reticulum. May contribute to tethering of coatomer-coated retrograde transport vesicles to the ER membrane through interaction with and stabilization of the SNARE complex. Ref.1 Ref.7
Subunit structure	Component of a peripheral membrane protein complex consisting of DSL1, SEC39/DSL3 and TIP20. Bound to a SNARE complex consisting of UFE1, USE1, SEC20 and SEC22 or YKT6 through direct interaction of TIP20 with SEC20. Interacts with DSL1, SEC39/DSL3 and the cytoplasmic domain of SEC20. Ref.1 Ref.6 Ref.7 Ref.8
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein Ref.1.
Miscellaneous	Present with 6020 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 RINT1/TIP20 domain.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Endoplasmic reticulum Membrane
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW retrograde vesicle-mediated transport, Golgi to ER Inferred from genetic interaction PubMed 9639310. Source: SGD
Cellular_component	Dsl1p complex Inferred from direct assay PubMed 19151722. Source: SGD endoplasmic reticulum Inferred from direct assay PubMed 11739780. Source: SGD endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
DSL1	P53847	7	EBI-19396,EBI-29249
SEC20	P28791	6	EBI-19396,EBI-16572
SEC39	Q12745	4	EBI-19396,EBI-31898

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 701

701

Protein transport protein TIP20

PRO_0000072543

Regions

Domain

147 – 701

555

RINT1/TIP20

Amino acid modifications

Modified residue

693

Phosphotyrosine Ref.9

Secondary structure

701

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P33891 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 3E561DD4C0B9C176
Show »

FASTA

701

81,167

        10         20         30         40         50         60 
MNGIDDLLNI NDRIKQVQNE RNELASKLQN LKQSLASNDT EVALSEVIAQ DIIEVGASVE 

        70         80         90        100        110        120 
GLEQLRAKYG DLQILNKLEK VAVQQTQMQA GVDKLDSFER QLDELAEQPP DQFTLDDVKA 

       130        140        150        160        170        180 
LHSKLTSVFA TVPQINNIDS QYAAYNKLKS KVTGKYNDVI IQRLATNWSN TFDQKLLEAQ 

       190        200        210        220        230        240 
WDTQKFASTS VGLVKCLREN STKLYQLSLL YLPLEEETQN GDSERPLSRS NNNQEPVLWN 

       250        260        270        280        290        300 
FKSLANNFNV RFTYHFHATS SSSKIETYFQ FLNDYLAENL YKCINIFHDD CNGLTKPVIH 

       310        320        330        340        350        360 
EQFINYVLQP IRDKVRSTLF QNDLKTLIVL ISQILATDKN LLNSFHYHGL GLVSLISDEV 

       370        380        390        400        410        420 
WEKWINYEVE MANRQFINIT KNPEDFPKSS QNFVKLINKI YDYLEPFYDL DFDLLVRYKL 

       430        440        450        460        470        480 
MTCSLIFMNL TSSYLDYILT VDSLNETRTK EQELYQTMAK LQHVNFVYRK IKSLSSNFIF 

       490        500        510        520        530        540 
IQLTDIVNST ESKKYNSLFQ NVENDYEKAM STDMQNSIVH RIQKLLKETL RNYFKISTWS 

       550        560        570        580        590        600 
TLEMSVDENI GPSSVPSAEL VNSINVLRRL INKLDSMDIP LAISLKVKNE LLNVIVNYFT 

       610        620        630        640        650        660 
ESILKLNKFN QNGLNQFLHD FKSLSSILSL PSHATNYKCM SLHELVKILK LKYDPNNQQF 

       670        680        690        700 
LNPEYIKTGN FTSLKEAYSI KYLKDTKIQD ALYRIIYGNI L

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The TIP1 gene of Saccharomyces cerevisiae encodes an 80 kDa cytoplasmic protein that interacts with the cytoplasmic domain of Sec20p." Sweet D.J., Pelham H.R.B. EMBO J. 12:2831-2840(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SEC20.
[2]	"The sequence of a nearly unclonable 22.8 kb segment on the left arm chromosome VII from Saccharomyces cerevisiae reveals ARO2, RPL9A, TIP1, MRF1 genes and six new open reading frames." Voet M., Defoor E., Verhasselt P., Riles L., Robben J., Volckaert G. Yeast 13:177-182(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Isolation of Saccharomyces cerevisiae RNase T1 hypersensitive (rns) mutants and genetic analysis of the RNS1/DSL1 gene." Ishikawa T., Unno K., Nonaka G., Nakajima H., Kitamoto K. J. Gen. Appl. Microbiol. 51:73-82(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SEC39.
[7]	"Dsl1p, Tip20p, and the novel Dsl3(Sec39) protein are required for the stability of the Q/t-SNARE complex at the endoplasmic reticulum in yeast." Kraynack B.A., Chan A., Rosenthal E., Essid M., Umansky B., Waters M.G., Schmitt H.D. Mol. Biol. Cell 16:3963-3977(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN A COMPLEX WITH DSL1; SEC39; SEC20; USE1 AND UFE1.
[8]	"Proteome survey reveals modularity of the yeast cell machinery." Gavin A.-C., Aloy P., Grandi P., Krause R., Boesche M., Marzioch M., Rau C., Jensen L.J., Bastuck S., Duempelfeld B., Edelmann A., Heurtier M.-A., Hoffman V., Hoefert C., Klein K., Hudak M., Michon A.-M., Schelder M. Superti-Furga G. Nature 440:631-636(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH DSL1; SEC39; SEC20; UFE1; USE1 AND SEC22.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-693, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X72699 Genomic DNA. Translation: CAA51249.1.
X99960 Genomic DNA. Translation: CAA68217.1.
Z72667 Genomic DNA. Translation: CAA96857.1.
BK006941 Genomic DNA. Translation: DAA07965.1.

PIR

S35313.

RefSeq

NP_116577.1. NM_001181010.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3ETV	X-ray	1.94	A	1-40	[»]
3FHN	X-ray	3.00	A/B/C/D	1-701	[»]

ProteinModelPortal

P33891.

SMR

P33891. Positions 5-701.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-2004N.

IntAct

P33891. 10 interactions.

MINT

MINT-403248.

STRING

4932.YGL145W.

Proteomic databases

PaxDb

P33891.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YGL145W; YGL145W; YGL145W.

GeneID

852732.

KEGG

sce:YGL145W.

Organism-specific databases

CYGD

YGL145w.

SGD

S000003113. TIP20.

Phylogenomic databases

eggNOG

NOG328678.

HOGENOM

HOG000000922.

OMA

RFNYHFN.

OrthoDB

EOG49S9G3.

Gene expression databases

Genevestigator

P33891.

GermOnline

YGL145W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR007528. RINT1_TIP1.
[Graphical view]

Pfam

PF04437. RINT1_TIP1. 1 hit.
[Graphical view]

PROSITE

PS51386. RINT1_TIP20. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P33891.

NextBio

972129.

Entry information

Entry name

TIP20_YEAST

Accession

Primary (citable) accession number: P33891
Secondary accession number(s): D6VU04

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents