P33887 (IF2G_RABIT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Eukaryotic translation initiation factor 2 subunit 3

Alternative name(s):
Eukaryotic translation initiation factor 2 subunit gamma
Short name=eIF-2-gamma

Gene names

Name:	EIF2S3
Synonyms:	EIF2G

Organism

Oryctolagus cuniculus (Rabbit) [Reference proteome]

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	152 AA.
Sequence status	Fragments.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Subunit structure	Heterotrimer composed of an alpha, a beta and a gamma chain.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EIF2G subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	GTP-binding Nucleotide-binding
Molecular function	Initiation factor
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Molecular_function	GTP binding Non-traceable author statement Ref.2Ref.3Ref.1. Source: UniProtKB translation initiation factor activity Non-traceable author statement Ref.2Ref.3Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›152	›152	Eukaryotic translation initiation factor 2 subunit 3		PRO_0000137442
Regions
	Nucleotide binding	51 – 54	4	GTP By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylalanine By similarity
Experimental info
	Sequence uncertainty	20	1
	Sequence conflict	3	1	G → T AA sequence Ref.1
	Sequence conflict	112	1	Q → W AA sequence Ref.2
	Non-adjacent residues	22 – 23	2
	Non-adjacent residues	29 – 30	2
	Non-adjacent residues	41 – 42	2
	Non-adjacent residues	81 – 82	2
	Non-adjacent residues	101 – 102	2
	Non-adjacent residues	137 – 138	2
	Non-terminal residue	152	1

Sequences

	Sequence		Length	Mass (Da)
	P33887 [UniParc]. Last modified May 27, 2002. Version 2. Checksum: CA56E544FA684CA7	FASTA	152	16,014
	10 20 30 40 50 60 AGGEAGVTLG QPSLVEQDSH SGFKNELERD GGLLLIAGNE SKLKHILILG NKIDLVKESQ 70 80 90 100 110 120 AKEQYGQILA FVQETVAXGA TVGQVLGAVG ALPEIFTELX IVNIGSLSTG GQVSAVKADL 130 140 150 GKIVLTNPVX TEVGEEKSVE KHWRLIGWGQ IR

References

[1]	"The NH2-terminal sequence of the alpha and gamma subunits of eukaryotic initiation factor 2 and the phosphorylation site for the heme-regulated eIF-2 alpha kinase." Wettenhall R.E.H., Kudlicki W., Kramer G., Hardesty B. J. Biol. Chem. 261:12444-12447(1986) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-22. Tissue: Reticulocyte.
[2]	"Amino acid sequence analysis of the beta- and gamma-subunits of eukaryotic initiation factor eIF-2. Identification of regions interacting with GTP." Bommer U.-A., Kraft R., Kurzchalia T.V., Price N.T., Proud C.G. Biochim. Biophys. Acta 1079:308-315(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-15 AND 82-126. Tissue: Reticulocyte.
[3]	"The purification and characterization of subunits alpha, beta, and gamma from the rabbit reticulocyte eukaryotic initiation factor 2." Schafer M.P., Fairwell T., Parker D.S., Knight M., Anderson W.F., Safer B. Arch. Biochem. Biophys. 255:337-346(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-8. Tissue: Reticulocyte.
[4]	"GCD11, a negative regulator of GCN4 expression, encodes the gamma subunit of eIF-2 in Saccharomyces cerevisiae." Hannig E.M., Cigan A.M., Freeman B.A., Kinzy T.G. Mol. Cell. Biol. 13:506-520(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 23-99 AND 102-152.

Cross-references

3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR015256. TIF2_gsu_C. IPR009001. Transl_elong_EF1A/Init_IF2_C. [Graphical view]
Pfam	PF09173. eIF2_C. 1 hit. [Graphical view]
SUPFAM	SSF50465. Elong_init_C. 1 hit.
ProtoNet	Search...

Entry information

Entry name

IF2G_RABIT

Accession

Primary (citable) accession number: P33887
Secondary accession number(s): Q9TRV8, Q9TRV9, Q9TRW0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	May 27, 2002
Last modified:	April 3, 2013
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program