Reviewed, UniProtKB/Swiss-Prot P33886 (WIS1_SCHPO)

Names and origin

Protein names

Recommended name:
    Protein kinase wis1
    EC=2.7.12.2
Alternative name(s):
    Protein kinase sty2

Gene names

Name:	wis1
Synonyms:	spc2, sty2
ORF Names:	SPBC409.07c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	605 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Dosage-dependent regulator of mitosis with serine/ threonine protein kinase activity. May play a role in the integration of nutritional sensing with the control over entry into mitosis. It may interact with cdc25, wee1 and win1. May activate sty1.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Post-translational modification	Dephosphorylated by pyp1 and pyp2. Ref.3
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Inferred from genetic interaction. Source: GeneDB_SPombe regulation of mitotic cell cycle Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe regulation of translation in response to stress Inferred from mutant phenotype. Source: GeneDB_SPombe response to arsenic Inferred from mutant phenotype. Source: GeneDB_SPombe response to hydrogen peroxide Inferred from mutant phenotype. Source: GeneDB_SPombe response to osmotic stress Traceable author statement. Source: GeneDB_SPombe stress-activated MAPK cascade Traceable author statement. Source: GeneDB_SPombe
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_SPombe nucleus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase kinase activity Inferred from genetic interaction. Source: GeneDB_SPombe protein binding Inferred from physical interaction. Source: GeneDB_SPombe protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 605	605	Protein kinase wis1		PRO_0000086817
Regions
	Domain	320 – 579	260	Protein kinase
	Nucleotide binding	326 – 334	9	ATP By similarity
Sites
	Active site	441	1	Proton acceptor By similarity
	Binding site	349	1	ATP By similarity
Amino acid modifications
	Modified residue	168	1	Phosphoserine Ref.3
	Modified residue	253	1	Phosphoserine Ref.3
	Modified residue	469	1	Phosphoserine By similarity
	Modified residue	473	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P33886-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 3EB97AF74190AD93	FASTA	605	64,762
	10 20 30 40 50 60 MSSPNNQPLS CSLRQLSISP TAPPGDVGTP GSLLSLSSSS SSNTDSSGSS LGSLSLNSNS 70 80 90 100 110 120 SGSDNDSKVS SPSREIPSDP PLPRAVPTVR LGRSTSSRSR NSLNLDMKDP SEKPRRSLPT 130 140 150 160 170 180 AAGQNNIGSP PTPPGPFPGG LSTDIQEKLK AFHASRSKSM PEVVNKISSP TTPIVGMGQR 190 200 210 220 230 240 GSYPLPNSQL AGRLSNSPVK SPNMPESGLA KSLAAARNPL LNRPTSFNRQ TRIRRAPPGK 250 260 270 280 290 300 LDLSNSNPTS PVSPSSMASR RGLNIPPTLK QAVSETPFST FSDILDAKSG TLNFKNKAVL 310 320 330 340 350 360 NSEGVNFSSG SSFRINMSEI IKLEELGKGN YGVVYKALHQ PTGVTMALKE IRLSLEEATF 370 380 390 400 410 420 NQIIMELDIL HKAVSPYIVD FYGAFFVEGS VFICMEYMDA GSMDKLYAGG IKDEGVLART 430 440 450 460 470 480 AYAVVQGLKT LKEEHNIIHR DVKPTNVLVN SNGQVKLCDF GVSGNLVASI SKTNIGCQSY 490 500 510 520 530 540 MAPERIRVGG PTNGVLTYTV QADVWSLGLT ILEMALGAYP YPPESYTSIF AQLSAICDGD 550 560 570 580 590 600 PPSLPDSFSP EARDFVNKCL NKNPSLRPDY HELANHPWLL KYQNADVDMA SWAKGALKEK GEKRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The wis1 protein kinase is a dosage-dependent regulator of mitosis in Schizosaccharomyces pombe." Warbrick E., Fantes P.A. EMBO J. 10:4291-4299(1991) [PubMed: 1756736] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 38366 / 972.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[3]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168 AND SER-253, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X62631 Genomic DNA. Translation: CAA44499.1. CU329671 Genomic DNA. Translation: CAB52609.1.
PIR	S18648.
RefSeq	NP_595457.1.
3D structure databases
HSSP	HSSP built from PDB template 1VR2 based on UniProtKB P35968.
ModBase	Search...
Protein-protein interaction databases
STRING	P33886.
Genome annotation databases
GeneID	2541055.
GenomeReviews	Gene locus wis1 in contig CU329671_GR.
KEGG	spo:SPBC409.07c.
NMPDR	fig|4896.1.peg.1323.
Organism-specific databases
GeneDB_Spombe	SPBC409.07c.
Phylogenomic databases
OMA	DYHELAN.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-003522-MON.
BRENDA	2.7.12.2. 653.
Gene expression databases
ArrayExpress	P33886.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

WIS1_SCHPO

Accession

Primary (citable) accession number: P33886

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	November 3, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)