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Protein

Protein kinase wis1

Gene

wis1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dosage-dependent regulator of mitosis with serine/ threonine protein kinase activity. May play a role in the integration of nutritional sensing with the control over entry into mitosis. It may interact with cdc25, wee1 and win1. May activate sty1.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei349 – 3491ATPPROSITE-ProRule annotation
Active sitei441 – 4411Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi326 – 3349ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase kinase activity Source: PomBase
  • non-membrane spanning protein tyrosine kinase activity Source: PomBase
  • protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  • activation of MAPK activity Source: GOC
  • cell division Source: UniProtKB-KW
  • MAPK cascade in response to starvation Source: PomBase
  • MAPK cascade involved in osmosensory signaling pathway Source: PomBase
  • mitotic G1 cell cycle arrest in response to nitrogen starvation Source: PomBase
  • mitotic nuclear division Source: UniProtKB-KW
  • peptidyl-tyrosine phosphorylation Source: PomBase
  • positive regulation of G2/M transition of mitotic cell cycle Source: PomBase
  • positive regulation of transcription factor import into nucleus in response to oxidative stress Source: PomBase
  • protein autophosphorylation Source: PomBase
  • protein phosphorylation Source: PomBase
  • regulation of cytoplasmic translation in response to stress Source: PomBase
  • stress-activated MAPK cascade Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.12.2. 5613.
ReactomeiR-SPO-168638. NOD1/2 Signaling Pathway.
R-SPO-2559580. Oxidative Stress Induced Senescence.
R-SPO-2871796. FCERI mediated MAPK activation.
R-SPO-450302. activated TAK1 mediates p38 MAPK activation.
R-SPO-450321. JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase wis1 (EC:2.7.12.2)
Alternative name(s):
Protein kinase sty2
Gene namesi
Name:wis1
Synonyms:spc2, sty2
ORF Names:SPBC409.07c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC409.07c.
PomBaseiSPBC409.07c. wis1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • Mcs4 RR-MAPKKK complex Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 605605Protein kinase wis1PRO_0000086817Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei168 – 1681Phosphoserine1 Publication
Modified residuei253 – 2531Phosphoserine1 Publication
Modified residuei469 – 4691PhosphoserineBy similarity
Modified residuei473 – 4731PhosphothreonineBy similarity

Post-translational modificationi

Dephosphorylated by pyp1 and pyp2.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP33886.

PTM databases

iPTMnetiP33886.

Interactioni

Protein-protein interaction databases

BioGridi277570. 36 interactions.
MINTiMINT-4688562.

Structurei

3D structure databases

ProteinModelPortaliP33886.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini320 – 579260Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000234206.
InParanoidiP33886.
KOiK11227.
OMAiSSFRINM.
OrthoDBiEOG7380F1.
PhylomeDBiP33886.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33886-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSPNNQPLS CSLRQLSISP TAPPGDVGTP GSLLSLSSSS SSNTDSSGSS
60 70 80 90 100
LGSLSLNSNS SGSDNDSKVS SPSREIPSDP PLPRAVPTVR LGRSTSSRSR
110 120 130 140 150
NSLNLDMKDP SEKPRRSLPT AAGQNNIGSP PTPPGPFPGG LSTDIQEKLK
160 170 180 190 200
AFHASRSKSM PEVVNKISSP TTPIVGMGQR GSYPLPNSQL AGRLSNSPVK
210 220 230 240 250
SPNMPESGLA KSLAAARNPL LNRPTSFNRQ TRIRRAPPGK LDLSNSNPTS
260 270 280 290 300
PVSPSSMASR RGLNIPPTLK QAVSETPFST FSDILDAKSG TLNFKNKAVL
310 320 330 340 350
NSEGVNFSSG SSFRINMSEI IKLEELGKGN YGVVYKALHQ PTGVTMALKE
360 370 380 390 400
IRLSLEEATF NQIIMELDIL HKAVSPYIVD FYGAFFVEGS VFICMEYMDA
410 420 430 440 450
GSMDKLYAGG IKDEGVLART AYAVVQGLKT LKEEHNIIHR DVKPTNVLVN
460 470 480 490 500
SNGQVKLCDF GVSGNLVASI SKTNIGCQSY MAPERIRVGG PTNGVLTYTV
510 520 530 540 550
QADVWSLGLT ILEMALGAYP YPPESYTSIF AQLSAICDGD PPSLPDSFSP
560 570 580 590 600
EARDFVNKCL NKNPSLRPDY HELANHPWLL KYQNADVDMA SWAKGALKEK

GEKRS
Length:605
Mass (Da):64,762
Last modified:February 1, 1994 - v1
Checksum:i3EB97AF74190AD93
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62631 Genomic DNA. Translation: CAA44499.1.
CU329671 Genomic DNA. Translation: CAB52609.1.
PIRiS18648.
RefSeqiNP_595457.1. NM_001021367.2.

Genome annotation databases

EnsemblFungiiSPBC409.07c.1; SPBC409.07c.1:pep; SPBC409.07c.
GeneIDi2541055.
KEGGispo:SPBC409.07c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62631 Genomic DNA. Translation: CAA44499.1.
CU329671 Genomic DNA. Translation: CAB52609.1.
PIRiS18648.
RefSeqiNP_595457.1. NM_001021367.2.

3D structure databases

ProteinModelPortaliP33886.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277570. 36 interactions.
MINTiMINT-4688562.

PTM databases

iPTMnetiP33886.

Proteomic databases

MaxQBiP33886.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC409.07c.1; SPBC409.07c.1:pep; SPBC409.07c.
GeneIDi2541055.
KEGGispo:SPBC409.07c.

Organism-specific databases

EuPathDBiFungiDB:SPBC409.07c.
PomBaseiSPBC409.07c. wis1.

Phylogenomic databases

HOGENOMiHOG000234206.
InParanoidiP33886.
KOiK11227.
OMAiSSFRINM.
OrthoDBiEOG7380F1.
PhylomeDBiP33886.

Enzyme and pathway databases

BRENDAi2.7.12.2. 5613.
ReactomeiR-SPO-168638. NOD1/2 Signaling Pathway.
R-SPO-2559580. Oxidative Stress Induced Senescence.
R-SPO-2871796. FCERI mediated MAPK activation.
R-SPO-450302. activated TAK1 mediates p38 MAPK activation.
R-SPO-450321. JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1.

Miscellaneous databases

PROiP33886.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The wis1 protein kinase is a dosage-dependent regulator of mitosis in Schizosaccharomyces pombe."
    Warbrick E., Fantes P.A.
    EMBO J. 10:4291-4299(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168 AND SER-253, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiWIS1_SCHPO
AccessioniPrimary (citable) accession number: P33886
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 8, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.