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Protein

Protein kinase wis1

Gene

wis1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Dosage-dependent regulator of mitosis with serine/ threonine protein kinase activity. May play a role in the integration of nutritional sensing with the control over entry into mitosis. It may interact with cdc25, wee1 and win1. May activate sty1.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei349ATPPROSITE-ProRule annotation1
Active sitei441Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi326 – 334ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase kinase activity Source: PomBase
  • protein kinase activity Source: PomBase
  • protein serine/threonine kinase activity Source: GO_Central

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • cellular response to heat Source: PomBase
  • cellular response to osmotic stress Source: PomBase
  • cellular response to oxidative stress Source: PomBase
  • positive regulation of G2/M transition of mitotic cell cycle Source: PomBase
  • positive regulation of protein import into nucleus Source: PomBase
  • regulation of apoptotic process Source: GO_Central
  • stress-activated MAPK cascade Source: PomBase

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, Cell division, Mitosis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.12.2 5613
ReactomeiR-SPO-168638 NOD1/2 Signaling Pathway
R-SPO-2559580 Oxidative Stress Induced Senescence
R-SPO-2871796 FCERI mediated MAPK activation
R-SPO-450302 activated TAK1 mediates p38 MAPK activation
R-SPO-450321 JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase wis1 (EC:2.7.12.2)
Alternative name(s):
Protein kinase sty2
Gene namesi
Name:wis1
Synonyms:spc2, sty2
ORF Names:SPBC409.07c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC409.07c
PomBaseiSPBC409.07c wis1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000868171 – 605Protein kinase wis1Add BLAST605

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei168Phosphoserine1 Publication1
Modified residuei253Phosphoserine1 Publication1
Modified residuei469PhosphoserineBy similarity1
Modified residuei473PhosphothreonineBy similarity1

Post-translational modificationi

Dephosphorylated by pyp1 and pyp2.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP33886
PaxDbiP33886
PRIDEiP33886

PTM databases

iPTMnetiP33886

Interactioni

Protein-protein interaction databases

BioGridi277570, 36 interactors
STRINGi4896.SPBC409.07c.1

Structurei

3D structure databases

ProteinModelPortaliP33886
SMRiP33886
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini320 – 579Protein kinasePROSITE-ProRule annotationAdd BLAST260

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000234206
InParanoidiP33886
KOiK11227
OMAiICMEYCN
OrthoDBiEOG092C3RSG
PhylomeDBiP33886

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

P33886-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSPNNQPLS CSLRQLSISP TAPPGDVGTP GSLLSLSSSS SSNTDSSGSS
60 70 80 90 100
LGSLSLNSNS SGSDNDSKVS SPSREIPSDP PLPRAVPTVR LGRSTSSRSR
110 120 130 140 150
NSLNLDMKDP SEKPRRSLPT AAGQNNIGSP PTPPGPFPGG LSTDIQEKLK
160 170 180 190 200
AFHASRSKSM PEVVNKISSP TTPIVGMGQR GSYPLPNSQL AGRLSNSPVK
210 220 230 240 250
SPNMPESGLA KSLAAARNPL LNRPTSFNRQ TRIRRAPPGK LDLSNSNPTS
260 270 280 290 300
PVSPSSMASR RGLNIPPTLK QAVSETPFST FSDILDAKSG TLNFKNKAVL
310 320 330 340 350
NSEGVNFSSG SSFRINMSEI IKLEELGKGN YGVVYKALHQ PTGVTMALKE
360 370 380 390 400
IRLSLEEATF NQIIMELDIL HKAVSPYIVD FYGAFFVEGS VFICMEYMDA
410 420 430 440 450
GSMDKLYAGG IKDEGVLART AYAVVQGLKT LKEEHNIIHR DVKPTNVLVN
460 470 480 490 500
SNGQVKLCDF GVSGNLVASI SKTNIGCQSY MAPERIRVGG PTNGVLTYTV
510 520 530 540 550
QADVWSLGLT ILEMALGAYP YPPESYTSIF AQLSAICDGD PPSLPDSFSP
560 570 580 590 600
EARDFVNKCL NKNPSLRPDY HELANHPWLL KYQNADVDMA SWAKGALKEK

GEKRS
Length:605
Mass (Da):64,762
Last modified:February 1, 1994 - v1
Checksum:i3EB97AF74190AD93
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62631 Genomic DNA Translation: CAA44499.1
CU329671 Genomic DNA Translation: CAB52609.1
PIRiS18648
RefSeqiNP_595457.1, NM_001021367.2

Genome annotation databases

EnsemblFungiiSPBC409.07c.1; SPBC409.07c.1:pep; SPBC409.07c
GeneIDi2541055
KEGGispo:SPBC409.07c

Similar proteinsi

Entry informationi

Entry nameiWIS1_SCHPO
AccessioniPrimary (citable) accession number: P33886
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: March 28, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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