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P33821 (E10_VAR67) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable FAD-linked sulfhydryl oxidase E10

EC=1.8.3.2
Gene names
ORF Names:E10R
OrganismVariola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus) [Reference proteome]
Taxonomic identifier587200 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length95 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. The complete pathway for formation of disulfide bonds in intracellular virion membrane proteins sequentially involves oxidation of E10R, A2.5 and G4 By similarity.

Catalytic activity

2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

Cofactor

FAD By similarity.

Subunit structure

Interacts with A2.5; this interaction involves formation of a transient disulfide-bonded intermediate, allowing disulfide bond transfer By similarity.

Subcellular location

Virion membrane By similarity. Note: Associated with crescent membranes, immature virions (IV) and mature virions (MV) By similarity.

Induction

Expressed in the late phase of the viral replicative cycle.

Sequence similarities

Belongs to the poxviridae E10 family.

Contains 1 ERV/ALR sulfhydryl oxidase domain.

Ontologies

Keywords
   Cellular componentMembrane
Viral envelope protein
Virion
   Developmental stageLate protein
   DomainRedox-active center
Transmembrane
Transmembrane helix
   LigandFAD
Flavoprotein
   Molecular functionOxidoreductase
   PTMDisulfide bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

viral envelope

Inferred from electronic annotation. Source: UniProtKB-KW

virion membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthiol oxidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 9595Probable FAD-linked sulfhydryl oxidase E10
PRO_0000099466

Regions

Topological domain1 – 88Intravirion Potential
Transmembrane9 – 2517Helical; Potential
Topological domain26 – 9570Virion surface Potential
Domain1 – 9595ERV/ALR sulfhydryl oxidase

Amino acid modifications

Disulfide bond43 ↔ 46Redox-active Potential

Sequences

Sequence LengthMass (Da)Tools
P33821 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 9BCB0B4306397E27

FASTA9510,836
        10         20         30         40         50         60 
MNPKHWGRAA WTIIFIVLSQ AGLDGNIEAC KRKLYTIVST LPCPACRRHA TIAIEDNNIM 

        70         80         90 
SSNDLNYIYY FFIRLFNNLA SDPKYAIDVS KVKPL 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the nucleotide sequence of a 43 kbp segment of the genome of variola virus India-1967 strain."
Shchelkunov S.N., Blinov V.M., Resenchuk S.M., Totmenin A.V., Sandakhchiev L.S.
Virus Res. 30:239-258(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Genes of variola and vaccinia viruses necessary to overcome the host protective mechanisms."
Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.
FEBS Lett. 319:80-83(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Potential virulence determinants in terminal regions of variola smallpox virus genome."
Massung R.F., Esposito J.J., Liu L.I., Qi J., Utterback T.R., Knight J.C., Aubin L., Yuran T.E., Parsons J.M., Loparev V.N., Selivanov N.A., Cavallaro K.F., Kerlavage A.R., Mahy B.W.J., Venter J.C.
Nature 366:748-751(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Bangladesh-1975.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X69198 Genomic DNA. Translation: CAA48992.1.
L22579 Genomic DNA. Translation: AAA60799.1.
PIRA72157.
D36842.
T28489.
RefSeqNP_042095.1. NC_001611.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1486405.

Family and domain databases

Gene3D1.20.120.310. 1 hit.
InterProIPR017905. ERV/ALR_sulphydryl_oxidase.
IPR006890. Sulphydryl_Oase_FAD-link_ERV1.
[Graphical view]
PfamPF04805. Pox_E10. 1 hit.
[Graphical view]
PIRSFPIRSF015696. VAC_E10R. 1 hit.
SUPFAMSSF69000. SSF69000. 1 hit.
PROSITEPS51324. ERV_ALR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE10_VAR67
AccessionPrimary (citable) accession number: P33821
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 14, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families