P33821 (E10R_VAR67) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable FAD-linked sulfhydryl oxidase E10
EC=1.8.3.2

Gene names

ORF Names:

E10R

Organism

Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus)

Taxonomic identifier

587200 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	95 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. The complete pathway for formation of disulfide bonds in intracellular virion membrane proteins sequentially involves oxydation of E10R, A2.5 and G4 By similarity.
Catalytic activity	2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.
Cofactor	FAD By similarity.
Subunit structure	Interacts with A2.5; this interaction involves formation of a transient disulfide-bonded intermediate, allowing disulfide bond transfer By similarity.
Subcellular location	Virion membrane By similarity. Note: Associated with crescent membranes, immature virions (IV) and mature virions (MV) By similarity.
Induction	Expressed in the late phase of the viral replicative cycle.
Sequence similarities	Belongs to the poxviridae E10 family. Contains 1 ERV/ALR sulfhydryl oxidase domain.

Ontologies

Keywords
Cellular component	Membrane Viral envelope protein Virion
Developmental stage	Late protein
Domain	Redox-active center Transmembrane Transmembrane helix
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiol oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 95	95	Probable FAD-linked sulfhydryl oxidase E10		PRO_0000099466
Regions
	Transmembrane	9 – 25	17	Helical; Potential
	Domain	1 – 95	95	ERV/ALR sulfhydryl oxidase
Amino acid modifications
	Disulfide bond	43 ↔ 46		Redox-active Potential

Sequences

	Sequence		Length	Mass (Da)
	P33821 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 9BCB0B4306397E27	FASTA	95	10,836
	10 20 30 40 50 60 MNPKHWGRAA WTIIFIVLSQ AGLDGNIEAC KRKLYTIVST LPCPACRRHA TIAIEDNNIM 70 80 90 SSNDLNYIYY FFIRLFNNLA SDPKYAIDVS KVKPL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the nucleotide sequence of a 43 kbp segment of the genome of variola virus India-1967 strain." Shchelkunov S.N., Blinov V.M., Resenchuk S.M., Totmenin A.V., Sandakhchiev L.S. Virus Res. 30:239-258(1993) [PubMed: 8109158] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genes of variola and vaccinia viruses necessary to overcome the host protective mechanisms." Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S. FEBS Lett. 319:80-83(1993) [PubMed: 8384129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Potential virulence determinants in terminal regions of variola smallpox virus genome." Massung R.F., Esposito J.J., Liu L.I., Qi J., Utterback T.R., Knight J.C., Aubin L., Yuran T.E., Parsons J.M., Loparev V.N., Selivanov N.A., Cavallaro K.F., Kerlavage A.R., Mahy B.W.J., Venter J.C. Nature 366:748-751(1993) [PubMed: 8264798] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Bangladesh-1975.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X69198 Genomic DNA. Translation: CAA48992.1. L22579 Genomic DNA. Translation: AAA60799.1.
PIR	A72157. D36842. T28489.
RefSeq	NP_042095.1. NC_001611.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1486405.
Phylogenomic databases
ProtClustDB	PHA3005.
Family and domain databases
InterPro	IPR017905. ERV/ALR_sulphydryl_oxidase. IPR006863. Evr1_Alr. IPR006890. Sulphydryl_Oase_FAD-link_ERV1. [Graphical view]
Gene3D	G3DSA:1.20.120.310. Evr1_Alr. 1 hit.
Pfam	PF04805. Pox_E10. 1 hit. [Graphical view]
PIRSF	PIRSF015696. VAC_E10R. 1 hit.
PROSITE	PS51324. ERV_ALR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E10R_VAR67

Accession

Primary (citable) accession number: P33821

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program