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P33821

- E10_VAR67

UniProt

P33821 - E10_VAR67

Protein

Probable FAD-linked sulfhydryl oxidase E10

Gene

E10R

Organism
Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
  1. Functioni

    FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. The complete pathway for formation of disulfide bonds in intracellular virion membrane proteins sequentially involves oxidation of E10R, A2.5 and G4 By similarity.PROSITE-ProRule annotation

    Catalytic activityi

    2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

    Cofactori

    FAD.PROSITE-ProRule annotation

    GO - Molecular functioni

    1. thiol oxidase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable FAD-linked sulfhydryl oxidase E10 (EC:1.8.3.2)
    Gene namesi
    ORF Names:E10R
    OrganismiVariola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus)
    Taxonomic identifieri587200 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000002060: Genome

    Subcellular locationi

    Virion membrane By similarity
    Note: Associated with crescent membranes, immature virions (IV) and mature virions (MV).By similarity

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. viral envelope Source: UniProtKB-KW
    3. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane, Viral envelope protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 9595Probable FAD-linked sulfhydryl oxidase E10PRO_0000099466Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi43 ↔ 46Redox-activePROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Inductioni

    Expressed in the late phase of the viral replicative cycle.

    Keywords - Developmental stagei

    Late protein

    Interactioni

    Subunit structurei

    Interacts with A2.5; this interaction involves formation of a transient disulfide-bonded intermediate, allowing disulfide bond transfer.By similarity

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 88IntravirionSequence Analysis
    Topological domaini26 – 9570Virion surfaceSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei9 – 2517HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 9595ERV/ALR sulfhydryl oxidasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the poxviridae E10 family.Curated
    Contains 1 ERV/ALR sulfhydryl oxidase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Redox-active center, Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di1.20.120.310. 1 hit.
    InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
    IPR006890. Sulphydryl_Oase_FAD-link_ERV1.
    [Graphical view]
    PfamiPF04805. Pox_E10. 1 hit.
    [Graphical view]
    PIRSFiPIRSF015696. VAC_E10R. 1 hit.
    SUPFAMiSSF69000. SSF69000. 1 hit.
    PROSITEiPS51324. ERV_ALR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P33821-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNPKHWGRAA WTIIFIVLSQ AGLDGNIEAC KRKLYTIVST LPCPACRRHA   50
    TIAIEDNNIM SSNDLNYIYY FFIRLFNNLA SDPKYAIDVS KVKPL 95
    Length:95
    Mass (Da):10,836
    Last modified:February 1, 1994 - v1
    Checksum:i9BCB0B4306397E27
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69198 Genomic DNA. Translation: CAA48992.1.
    L22579 Genomic DNA. Translation: AAA60799.1.
    PIRiA72157.
    D36842.
    T28489.
    RefSeqiNP_042095.1. NC_001611.1.

    Genome annotation databases

    GeneIDi1486405.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69198 Genomic DNA. Translation: CAA48992.1 .
    L22579 Genomic DNA. Translation: AAA60799.1 .
    PIRi A72157.
    D36842.
    T28489.
    RefSeqi NP_042095.1. NC_001611.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1486405.

    Family and domain databases

    Gene3Di 1.20.120.310. 1 hit.
    InterProi IPR017905. ERV/ALR_sulphydryl_oxidase.
    IPR006890. Sulphydryl_Oase_FAD-link_ERV1.
    [Graphical view ]
    Pfami PF04805. Pox_E10. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF015696. VAC_E10R. 1 hit.
    SUPFAMi SSF69000. SSF69000. 1 hit.
    PROSITEi PS51324. ERV_ALR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the nucleotide sequence of a 43 kbp segment of the genome of variola virus India-1967 strain."
      Shchelkunov S.N., Blinov V.M., Resenchuk S.M., Totmenin A.V., Sandakhchiev L.S.
      Virus Res. 30:239-258(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Genes of variola and vaccinia viruses necessary to overcome the host protective mechanisms."
      Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.
      FEBS Lett. 319:80-83(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Bangladesh-1975.

    Entry informationi

    Entry nameiE10_VAR67
    AccessioniPrimary (citable) accession number: P33821
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3