Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P33821

- E10_VAR67

UniProt

P33821 - E10_VAR67

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Probable FAD-linked sulfhydryl oxidase E10

Gene

E10R

Organism
Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. The complete pathway for formation of disulfide bonds in intracellular virion membrane proteins sequentially involves oxidation of E10R, A2.5 and G4 (By similarity).PROSITE-ProRule annotation

Catalytic activityi

2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

Cofactori

FAD.PROSITE-ProRule annotation

GO - Molecular functioni

  1. thiol oxidase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Probable FAD-linked sulfhydryl oxidase E10 (EC:1.8.3.2)
Gene namesi
ORF Names:E10R
OrganismiVariola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus)
Taxonomic identifieri587200 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000002060: Genome

Subcellular locationi

Virion membrane By similarity
Note: Associated with crescent membranes, immature virions (IV) and mature virions (MV).By similarity

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. viral envelope Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9595Probable FAD-linked sulfhydryl oxidase E10PRO_0000099466Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi43 ↔ 46Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Interacts with A2.5; this interaction involves formation of a transient disulfide-bonded intermediate, allowing disulfide bond transfer.By similarity

Structurei

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88IntravirionSequence Analysis
Topological domaini26 – 9570Virion surfaceSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei9 – 2517HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 9595ERV/ALR sulfhydryl oxidasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the poxviridae E10 family.Curated
Contains 1 ERV/ALR sulfhydryl oxidase domain.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.20.120.310. 1 hit.
InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
IPR006890. Sulphydryl_Oase_FAD-link_ERV1.
[Graphical view]
PfamiPF04805. Pox_E10. 1 hit.
[Graphical view]
PIRSFiPIRSF015696. VAC_E10R. 1 hit.
SUPFAMiSSF69000. SSF69000. 1 hit.
PROSITEiPS51324. ERV_ALR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33821-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNPKHWGRAA WTIIFIVLSQ AGLDGNIEAC KRKLYTIVST LPCPACRRHA
60 70 80 90
TIAIEDNNIM SSNDLNYIYY FFIRLFNNLA SDPKYAIDVS KVKPL
Length:95
Mass (Da):10,836
Last modified:February 1, 1994 - v1
Checksum:i9BCB0B4306397E27
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X69198 Genomic DNA. Translation: CAA48992.1.
L22579 Genomic DNA. Translation: AAA60799.1.
PIRiA72157.
D36842.
T28489.
RefSeqiNP_042095.1. NC_001611.1.

Genome annotation databases

GeneIDi1486405.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X69198 Genomic DNA. Translation: CAA48992.1 .
L22579 Genomic DNA. Translation: AAA60799.1 .
PIRi A72157.
D36842.
T28489.
RefSeqi NP_042095.1. NC_001611.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1486405.

Family and domain databases

Gene3Di 1.20.120.310. 1 hit.
InterProi IPR017905. ERV/ALR_sulphydryl_oxidase.
IPR006890. Sulphydryl_Oase_FAD-link_ERV1.
[Graphical view ]
Pfami PF04805. Pox_E10. 1 hit.
[Graphical view ]
PIRSFi PIRSF015696. VAC_E10R. 1 hit.
SUPFAMi SSF69000. SSF69000. 1 hit.
PROSITEi PS51324. ERV_ALR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the nucleotide sequence of a 43 kbp segment of the genome of variola virus India-1967 strain."
    Shchelkunov S.N., Blinov V.M., Resenchuk S.M., Totmenin A.V., Sandakhchiev L.S.
    Virus Res. 30:239-258(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Genes of variola and vaccinia viruses necessary to overcome the host protective mechanisms."
    Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.
    FEBS Lett. 319:80-83(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Bangladesh-1975.

Entry informationi

Entry nameiE10_VAR67
AccessioniPrimary (citable) accession number: P33821
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 29, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3