P33809 (PAP1_VAR67) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Poly(A) polymerase catalytic subunit
EC=2.7.7.19

Alternative name(s):
Poly(A) polymerase large subunit
Short name=PAP-L
VP55

Gene names

Name:	PAPL
ORF Names:	C1L, E1L

Organism

Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus) [Reference proteome]

Taxonomic identifier

587200 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Variola virus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Polymerase that creates the 3'-poly(A) tail of mRNA's.
Catalytic activity	ATP + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Heterodimer of a large (catalytic) subunit and a small (regulatory) subunit.
Sequence similarities	Belongs to the poxviridae poly(A) polymerase catalytic subunit family.

Ontologies

Keywords
Biological process	Transcription mRNA processing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW polynucleotide adenylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 479	479	Poly(A) polymerase catalytic subunit		PRO_0000099108
Sites
	Active site	202	1	By similarity
	Active site	204	1	By similarity
Natural variations
	Natural variant	10	1	F → L in strain: Bangladesh-1975 and Garcia-1966.
	Natural variant	110	1	N → D in strain: Bangladesh-1975 and Garcia-1966.

Sequences

	Sequence		Length	Mass (Da)
	P33809 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 5118681BFC93DDE1	FASTA	479	55,586
	10 20 30 40 50 60 MNRNPDHNTF PNITLKIIET YLGRLPSVNE YHMLKLQTRN IQKITVFNKD IFVSLVKKNK 70 80 90 100 110 120 KRFFSDVDTS ASEIKDRILS YFSKQTQTYN IGKLFTIIEL QSVLVTTYTN ILGVLTIKAP 130 140 150 160 170 180 NVISSKISYN VTSMEELARD MLNSMNVAVI DKAKVMGRHN VSSLVKNVNK LMEEYLRRHN 190 200 210 220 230 240 KSCICYGSYS LYLINPNIRY GDIDILQTNS RTFLIDLAFL IKFITGNNII LSKIPYLRNY 250 260 270 280 290 300 MVIKDENDNH IIDSFNIRQD TMNVVPKIFI DNIYIVDPTF QLLNMIKMFS QIDRLEDLSK 310 320 330 340 350 360 DPEKFNARMA TMLEYVRYTH GIVFDGKRNN MPMKCIIDEN NRVVTVTTKD YFSFKKCLVY 370 380 390 400 410 420 LDENVLSSDI LDLNADTSCD FESVTNSVYL IHDNIMYTYF SNTILLSDKG KVHEISARGL 430 440 450 460 470 CAHILLYQML TSGAYKQCLS DLLNSMMNRD KIPIYSHTER DKKHGRHGFI NIEKDIIVF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the nucleotide sequence of a 43 kbp segment of the genome of variola virus India-1967 strain." Shchelkunov S.N., Blinov V.M., Resenchuk S.M., Totmenin A.V., Sandakhchiev L.S. Virus Res. 30:239-258(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: India-1967 / Isolate Ind3.
[2]	"Genes of variola and vaccinia viruses necessary to overcome the host protective mechanisms." Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S. FEBS Lett. 319:80-83(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: India-1967 / Isolate Ind3.
[3]	"Potential virulence determinants in terminal regions of variola smallpox virus genome." Massung R.F., Esposito J.J., Liu L.I., Qi J., Utterback T.R., Knight J.C., Aubin L., Yuran T.E., Parsons J.M., Loparev V.N., Selivanov N.A., Cavallaro K.F., Kerlavage A.R., Mahy B.W.J., Venter J.C. Nature 366:748-751(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Bangladesh-1975.
[4]	"Alastrim smallpox variola minor virus genome DNA sequences." Shchelkunov S.N., Totmenin A.V., Loparev V.N., Safronov P.F., Gutorov V.V., Chizhikov V.E., Knight J.C., Parsons J.M., Massung R.F., Esposito J.J. Virology 266:361-386(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Garcia-1966.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X69198 Genomic DNA. Translation: CAA48983.1. L22579 Genomic DNA. Translation: AAA60790.1. Y16780 Genomic DNA. Translation: CAB54642.1.
PIR	D36841. H72155. T28480.
RefSeq	NP_042086.1. NC_001611.1.
3D structure databases
ProteinModelPortal	P33809.
SMR	P33809. Positions 12-479.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1486407.
Phylogenomic databases
ProtClustDB	PHA2996.
Family and domain databases
InterPro	IPR024231. Pox_polyA_pol_nucTrfase. IPR004976. Poxvirus_polyA_pol_cat. IPR024397. Poxvirus_polyA_pol_cat_C. IPR024398. Poxvirus_polyA_pol_cat_N. [Graphical view]
Pfam	PF03296. Pox_polyA_pol. 1 hit. PF12629. Pox_polyA_pol_C. 1 hit. PF12630. Pox_polyA_pol_N. 1 hit. [Graphical view]
PIRSF	PIRSF015693. VAC-48L_nuct. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PAP1_VAR67

Accession

Primary (citable) accession number: P33809
Secondary accession number(s): Q76Q36, Q85370

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program