Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pro-variola growth factor

Gene

B3R

Organism
Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Variola growth factor stimulates cellular proliferation (hyperplasia) around infected cells. This effect is beneficial for virus replication in vivo, because poxviruses replicate possibly better in proliferating cells than in quiescent cells. Acts by binding host EGFR, inducing its dimerization, autophosphorylation and leading to activation of several cellular pathways regulating cell proliferation or cell survival. The activation by host EFGR of mitogen activated protein kinases (MAPK) and extracellular-signal regulated kinases (ERK) are essential for the positive effect of vaccinia growth factor on poxvirus virulence in vivo (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Growth factor

Keywords - Biological processi

Host-virus interaction

Names & Taxonomyi

Protein namesi
Recommended name:
Pro-variola growth factor
Short name:
Pro-VGF
Cleaved into the following chain:
Variola growth factor
Short name:
VGF
Alternative name(s):
Secreted epidermal growth factor-like
Gene namesi
ORF Names:B3R, B4R, C11R, D2L, D4R
OrganismiVariola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus)
Taxonomic identifieri587200 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000002060 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini19 – 10082ExtracellularSequence analysisAdd
BLAST
Transmembranei101 – 12121HelicalSequence analysisAdd
BLAST
Topological domaini122 – 14019CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence analysisAdd
BLAST
Chaini19 – 140122Pro-variola growth factorPRO_0000007600Add
BLAST
Chaini19 – 9678Variola growth factorSequence analysisPRO_0000412919Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi34 – 341N-linked (GlcNAc...); by hostSequence analysis
Disulfide bondi45 ↔ 58PROSITE-ProRule annotation
Disulfide bondi53 ↔ 69PROSITE-ProRule annotation
Disulfide bondi71 ↔ 80PROSITE-ProRule annotation
Glycosylationi95 – 951N-linked (GlcNAc...); by hostSequence analysis

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei96 – 972Cleavage (By host protease)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Variola growth factor interacts with host EGFR and promotes EGFR dimerization.By similarity

Structurei

3D structure databases

ProteinModelPortaliP33804.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 8141EGF-likePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 EGF-like domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK09784.

Family and domain databases

InterProiIPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR015497. EGF_rcpt_ligand.
IPR011170. GF_C11R.
[Graphical view]
PANTHERiPTHR10740. PTHR10740. 1 hit.
PIRSFiPIRSF001779. GF_C11R. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33804-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMKYLMLLF AAMIIRSFAN SGNAIETTLS EITNTTTDIP AIRLCGPEGD
60 70 80 90 100
RYCFHGICIH ARDIDGMYCR CSHGYTGIRC QHVVLVDYQR SEKPNTTTSY
110 120 130 140
IPSPGIVLVL LVSIIVCCLL FVYRFTRRTN KLPLQDMVVP
Length:140
Mass (Da):15,772
Last modified:June 15, 2010 - v3
Checksum:i03A134EA5A94E14A
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti50 – 512DR → NG in strain: Bangladesh-1975, Congo-1965, Garcia-1966 and Somalia-1977.
Natural varianti113 – 1131S → G in strain: Garcia-1966.
Natural varianti116 – 1161V → M in strain: Bangladesh-1975, Congo-1965, Garcia-1966 and Somalia-1977.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69198 Genomic DNA. Translation: CAA48943.1.
L22579 Genomic DNA. Translation: AAA60749.1.
U18337 Genomic DNA. Translation: AAA69306.1.
U18338 Genomic DNA. Translation: AAA69343.1.
U18340 Genomic DNA. Translation: AAA69412.1.
Y16780 Genomic DNA. Translation: CAB54597.1.
PIRiB36837.
C72150.
T28439.
RefSeqiNP_042046.1. NC_001611.1.

Genome annotation databases

GeneIDi1486396.
KEGGivg:1486396.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69198 Genomic DNA. Translation: CAA48943.1.
L22579 Genomic DNA. Translation: AAA60749.1.
U18337 Genomic DNA. Translation: AAA69306.1.
U18338 Genomic DNA. Translation: AAA69343.1.
U18340 Genomic DNA. Translation: AAA69412.1.
Y16780 Genomic DNA. Translation: CAB54597.1.
PIRiB36837.
C72150.
T28439.
RefSeqiNP_042046.1. NC_001611.1.

3D structure databases

ProteinModelPortaliP33804.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1486396.
KEGGivg:1486396.

Phylogenomic databases

KOiK09784.

Family and domain databases

InterProiIPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR015497. EGF_rcpt_ligand.
IPR011170. GF_C11R.
[Graphical view]
PANTHERiPTHR10740. PTHR10740. 1 hit.
PIRSFiPIRSF001779. GF_C11R. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genes of variola and vaccinia viruses necessary to overcome the host protective mechanisms."
    Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.
    FEBS Lett. 319:80-83(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: India-1967 / Isolate Ind3.
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Bangladesh-1975.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Congo-1965, Garcia-1966 and Somalia-1977.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Garcia-1966.

Entry informationi

Entry nameiVGF_VAR67
AccessioniPrimary (citable) accession number: P33804
Secondary accession number(s): Q76Q77
, Q76UB3, Q89066, Q89756
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: June 15, 2010
Last modified: May 11, 2016
This is version 82 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.