P33799 (RIR2_VAR67) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonucleoside-diphosphate reductase small chain
EC=1.17.4.1

Alternative name(s):
Ribonucleotide reductase small subunit

Gene names

ORF Names:

C8L, F4L

Organism

Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus) [Reference proteome]

Taxonomic identifier

587200 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Variola virus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Cofactor	Binds 2 iron ions per subunit By similarity.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.
Induction	Expressed early in the viral replicative cycle.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase small chain family.
Sequence caution	The sequence AAA60776.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA48969.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	DNA replication
Developmental stage	Early protein
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-UniPathway deoxyribonucleoside diphosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Inferred from electronic annotation. Source: EC transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	Ribonucleoside-diphosphate reductase small chain		PRO_0000190499
Sites
	Active site	108	1	By similarity
	Metal binding	70	1	Iron 1 By similarity
	Metal binding	101	1	Iron 1 By similarity
	Metal binding	101	1	Iron 2 By similarity
	Metal binding	104	1	Iron 1 By similarity
	Metal binding	163	1	Iron 2 By similarity
	Metal binding	197	1	Iron 2 By similarity
	Metal binding	200	1	Iron 2 By similarity
Natural variations
	Natural variant	151	1	G → S in strain: Bangladesh-1975.
	Natural variant	255 – 256	2	ME → IK in strain: Bangladesh-1975.
	Natural variant	270	1	R → K in strain: Bangladesh-1975.
	Natural variant	278	1	S → F in strain: Bangladesh-1975.

Sequences

	Sequence		Length	Mass (Da)
	P33799 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: E0D62D56C4894928	FASTA	319	37,031
	10 20 30 40 50 60 MEPILAKNPN RFVIFPIQYH DIWNMYKKAE ASFWTVEEVD ISKDINDWNK LTPDEKYFIK 70 80 90 100 110 120 HVLAFFAASD GIVNENLAER FCIEVQITEA RCFYGFQMAI ENIHSEMYSL LIDTYVKDSN 130 140 150 160 170 180 EKNYLFNAIE TMPCVKKKAD WAQKWIHDSA GYGERLIAFA AVEGIFFSGS FASIFWLKKR 190 200 210 220 230 240 GLMPGLTFSN ELISRDEGLH CDFACLMFKH LLYPPSEETV RSIITDAVSI EQEFLTVALP 250 260 270 280 290 300 VKLIGMNCEM MKTYMEFVAD RLISELGFKR IYNVTNPSDF MENISLEGKT NFFEKRVGEY 310 QKMGVMSQED NHFSLDVDF

References

[1]	"Analysis of the nucleotide sequence of a 43 kbp segment of the genome of variola virus India-1967 strain." Shchelkunov S.N., Blinov V.M., Resenchuk S.M., Totmenin A.V., Sandakhchiev L.S. Virus Res. 30:239-258(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: India-1967 / Isolate Ind3.
[2]	"Genes of variola and vaccinia viruses necessary to overcome the host protective mechanisms." Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S. FEBS Lett. 319:80-83(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: India-1967 / Isolate Ind3.
[3]	"Potential virulence determinants in terminal regions of variola smallpox virus genome." Massung R.F., Esposito J.J., Liu L.I., Qi J., Utterback T.R., Knight J.C., Aubin L., Yuran T.E., Parsons J.M., Loparev V.N., Selivanov N.A., Cavallaro K.F., Kerlavage A.R., Mahy B.W.J., Venter J.C. Nature 366:748-751(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Bangladesh-1975.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X69198 Genomic DNA. Translation: CAA48969.1. Different initiation. L22579 Genomic DNA. Translation: AAA60776.1. Different initiation.
PIR	H36839. T28466.
RefSeq	NP_042072.2. NC_001611.1.
3D structure databases
ProteinModelPortal	P33799.
SMR	P33799. Positions 2-282.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1486388.
Phylogenomic databases
ProtClustDB	CLSP2509773.
Enzyme and pathway databases
UniPathway	UPA00326.
Family and domain databases
Gene3D	1.10.620.20. 1 hit.
InterPro	IPR009078. Ferritin-like_SF. IPR012348. RNR-rel. IPR000358. RNR_small. [Graphical view]
PANTHER	PTHR23409. PTHR23409. 1 hit.
Pfam	PF00268. Ribonuc_red_sm. 1 hit. [Graphical view]
SUPFAM	SSF47240. Ferritin/RR_like. 1 hit.
PROSITE	PS00368. RIBORED_SMALL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIR2_VAR67

Accession

Primary (citable) accession number: P33799

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program