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P33799

- RIR2_VAR67

UniProt

P33799 - RIR2_VAR67

Protein

Ribonucleoside-diphosphate reductase small chain

Gene

C8L

Organism
Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.By similarity

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

    Cofactori

    Binds 2 iron ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi70 – 701Iron 1PROSITE-ProRule annotation
    Metal bindingi101 – 1011Iron 1PROSITE-ProRule annotation
    Metal bindingi101 – 1011Iron 2By similarity
    Metal bindingi104 – 1041Iron 1PROSITE-ProRule annotation
    Active sitei108 – 1081PROSITE-ProRule annotation
    Metal bindingi163 – 1631Iron 2By similarity
    Metal bindingi197 – 1971Iron 2By similarity
    Metal bindingi200 – 2001Iron 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
    2. DNA replication Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00326.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonucleoside-diphosphate reductase small chain (EC:1.17.4.1)
    Alternative name(s):
    Ribonucleotide reductase small subunit
    Gene namesi
    ORF Names:C8L, F4L
    OrganismiVariola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus)
    Taxonomic identifieri587200 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000002060: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 319319Ribonucleoside-diphosphate reductase small chainPRO_0000190499Add
    BLAST

    Expressioni

    Inductioni

    Expressed early in the viral replicative cycle.

    Keywords - Developmental stagei

    Early protein

    Interactioni

    Subunit structurei

    Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP33799.
    SMRiP33799. Positions 2-282.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di1.10.620.20. 1 hit.
    InterProiIPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR000358. RNR_small.
    [Graphical view]
    PANTHERiPTHR23409. PTHR23409. 1 hit.
    PfamiPF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view]
    SUPFAMiSSF47240. SSF47240. 1 hit.
    PROSITEiPS00368. RIBORED_SMALL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P33799-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEPILAKNPN RFVIFPIQYH DIWNMYKKAE ASFWTVEEVD ISKDINDWNK    50
    LTPDEKYFIK HVLAFFAASD GIVNENLAER FCIEVQITEA RCFYGFQMAI 100
    ENIHSEMYSL LIDTYVKDSN EKNYLFNAIE TMPCVKKKAD WAQKWIHDSA 150
    GYGERLIAFA AVEGIFFSGS FASIFWLKKR GLMPGLTFSN ELISRDEGLH 200
    CDFACLMFKH LLYPPSEETV RSIITDAVSI EQEFLTVALP VKLIGMNCEM 250
    MKTYMEFVAD RLISELGFKR IYNVTNPSDF MENISLEGKT NFFEKRVGEY 300
    QKMGVMSQED NHFSLDVDF 319
    Length:319
    Mass (Da):37,031
    Last modified:February 1, 1994 - v1
    Checksum:iE0D62D56C4894928
    GO

    Sequence cautioni

    The sequence AAA60776.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA48969.1 differs from that shown. Reason: Erroneous initiation.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti151 – 1511G → S in strain: Bangladesh-1975.
    Natural varianti255 – 2562ME → IK in strain: Bangladesh-1975.
    Natural varianti270 – 2701R → K in strain: Bangladesh-1975.
    Natural varianti278 – 2781S → F in strain: Bangladesh-1975.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69198 Genomic DNA. Translation: CAA48969.1. Different initiation.
    L22579 Genomic DNA. Translation: AAA60776.1. Different initiation.
    PIRiH36839.
    T28466.
    RefSeqiNP_042072.2. NC_001611.1.

    Genome annotation databases

    GeneIDi1486388.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69198 Genomic DNA. Translation: CAA48969.1 . Different initiation.
    L22579 Genomic DNA. Translation: AAA60776.1 . Different initiation.
    PIRi H36839.
    T28466.
    RefSeqi NP_042072.2. NC_001611.1.

    3D structure databases

    ProteinModelPortali P33799.
    SMRi P33799. Positions 2-282.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1486388.

    Enzyme and pathway databases

    UniPathwayi UPA00326 .

    Family and domain databases

    Gene3Di 1.10.620.20. 1 hit.
    InterProi IPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR000358. RNR_small.
    [Graphical view ]
    PANTHERi PTHR23409. PTHR23409. 1 hit.
    Pfami PF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47240. SSF47240. 1 hit.
    PROSITEi PS00368. RIBORED_SMALL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the nucleotide sequence of a 43 kbp segment of the genome of variola virus India-1967 strain."
      Shchelkunov S.N., Blinov V.M., Resenchuk S.M., Totmenin A.V., Sandakhchiev L.S.
      Virus Res. 30:239-258(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: India-1967 / Isolate Ind3.
    2. "Genes of variola and vaccinia viruses necessary to overcome the host protective mechanisms."
      Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.
      FEBS Lett. 319:80-83(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: India-1967 / Isolate Ind3.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Bangladesh-1975.

    Entry informationi

    Entry nameiRIR2_VAR67
    AccessioniPrimary (citable) accession number: P33799
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3