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P33799 (RIR2_VAR67) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonucleoside-diphosphate reductase small chain

EC=1.17.4.1
Alternative name(s):
Ribonucleotide reductase small subunit
Gene names
ORF Names:C8L, F4L
OrganismVariola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus) [Reference proteome]
Taxonomic identifier587200 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length319 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activity

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactor

Binds 2 iron ions per subunit By similarity.

Pathway

Genetic information processing; DNA replication.

Subunit structure

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.

Induction

Expressed early in the viral replicative cycle.

Sequence similarities

Belongs to the ribonucleoside diphosphate reductase small chain family.

Sequence caution

The sequence AAA60776.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAA48969.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 319319Ribonucleoside-diphosphate reductase small chain
PRO_0000190499

Sites

Active site1081 By similarity
Metal binding701Iron 1 By similarity
Metal binding1011Iron 1 By similarity
Metal binding1011Iron 2 By similarity
Metal binding1041Iron 1 By similarity
Metal binding1631Iron 2 By similarity
Metal binding1971Iron 2 By similarity
Metal binding2001Iron 2 By similarity

Natural variations

Natural variant1511G → S in strain: Bangladesh-1975.
Natural variant255 – 2562ME → IK in strain: Bangladesh-1975.
Natural variant2701R → K in strain: Bangladesh-1975.
Natural variant2781S → F in strain: Bangladesh-1975.

Sequences

Sequence LengthMass (Da)Tools
P33799 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: E0D62D56C4894928

FASTA31937,031
        10         20         30         40         50         60 
MEPILAKNPN RFVIFPIQYH DIWNMYKKAE ASFWTVEEVD ISKDINDWNK LTPDEKYFIK 

        70         80         90        100        110        120 
HVLAFFAASD GIVNENLAER FCIEVQITEA RCFYGFQMAI ENIHSEMYSL LIDTYVKDSN 

       130        140        150        160        170        180 
EKNYLFNAIE TMPCVKKKAD WAQKWIHDSA GYGERLIAFA AVEGIFFSGS FASIFWLKKR 

       190        200        210        220        230        240 
GLMPGLTFSN ELISRDEGLH CDFACLMFKH LLYPPSEETV RSIITDAVSI EQEFLTVALP 

       250        260        270        280        290        300 
VKLIGMNCEM MKTYMEFVAD RLISELGFKR IYNVTNPSDF MENISLEGKT NFFEKRVGEY 

       310 
QKMGVMSQED NHFSLDVDF 

« Hide

References

[1]"Analysis of the nucleotide sequence of a 43 kbp segment of the genome of variola virus India-1967 strain."
Shchelkunov S.N., Blinov V.M., Resenchuk S.M., Totmenin A.V., Sandakhchiev L.S.
Virus Res. 30:239-258(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: India-1967 / Isolate Ind3.
[2]"Genes of variola and vaccinia viruses necessary to overcome the host protective mechanisms."
Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.
FEBS Lett. 319:80-83(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: India-1967 / Isolate Ind3.
[3]"Potential virulence determinants in terminal regions of variola smallpox virus genome."
Massung R.F., Esposito J.J., Liu L.I., Qi J., Utterback T.R., Knight J.C., Aubin L., Yuran T.E., Parsons J.M., Loparev V.N., Selivanov N.A., Cavallaro K.F., Kerlavage A.R., Mahy B.W.J., Venter J.C.
Nature 366:748-751(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Bangladesh-1975.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X69198 Genomic DNA. Translation: CAA48969.1. Different initiation.
L22579 Genomic DNA. Translation: AAA60776.1. Different initiation.
PIRH36839.
T28466.
RefSeqNP_042072.2. NC_001611.1.

3D structure databases

ProteinModelPortalP33799.
SMRP33799. Positions 2-282.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1486388.

Enzyme and pathway databases

UniPathwayUPA00326.

Family and domain databases

Gene3D1.10.620.20. 1 hit.
InterProIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view]
PANTHERPTHR23409. PTHR23409. 1 hit.
PfamPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMSSF47240. SSF47240. 1 hit.
PROSITEPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRIR2_VAR67
AccessionPrimary (citable) accession number: P33799
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 14, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways