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P33799

- RIR2_VAR67

UniProt

P33799 - RIR2_VAR67

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Protein
Ribonucleoside-diphosphate reductase small chain
Gene
C8L, F4L
Organism
Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactori

Binds 2 iron ions per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701Iron 1 By similarity
Metal bindingi101 – 1011Iron 1 By similarity
Metal bindingi101 – 1011Iron 2 By similarity
Metal bindingi104 – 1041Iron 1 By similarity
Active sitei108 – 1081 By similarity
Metal bindingi163 – 1631Iron 2 By similarity
Metal bindingi197 – 1971Iron 2 By similarity
Metal bindingi200 – 2001Iron 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
  2. deoxyribonucleoside diphosphate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase small chain (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase small subunit
Gene namesi
ORF Names:C8L, F4L
OrganismiVariola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus)
Taxonomic identifieri587200 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000002060: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 319319Ribonucleoside-diphosphate reductase small chain
PRO_0000190499Add
BLAST

Expressioni

Inductioni

Expressed early in the viral replicative cycle.

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.

Structurei

3D structure databases

ProteinModelPortaliP33799.
SMRiP33799. Positions 2-282.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33799-1 [UniParc]FASTAAdd to Basket

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MEPILAKNPN RFVIFPIQYH DIWNMYKKAE ASFWTVEEVD ISKDINDWNK    50
LTPDEKYFIK HVLAFFAASD GIVNENLAER FCIEVQITEA RCFYGFQMAI 100
ENIHSEMYSL LIDTYVKDSN EKNYLFNAIE TMPCVKKKAD WAQKWIHDSA 150
GYGERLIAFA AVEGIFFSGS FASIFWLKKR GLMPGLTFSN ELISRDEGLH 200
CDFACLMFKH LLYPPSEETV RSIITDAVSI EQEFLTVALP VKLIGMNCEM 250
MKTYMEFVAD RLISELGFKR IYNVTNPSDF MENISLEGKT NFFEKRVGEY 300
QKMGVMSQED NHFSLDVDF 319
Length:319
Mass (Da):37,031
Last modified:February 1, 1994 - v1
Checksum:iE0D62D56C4894928
GO

Sequence cautioni

The sequence AAA60776.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAA48969.1 differs from that shown. Reason: Erroneous initiation.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti151 – 1511G → S in strain: Bangladesh-1975.
Natural varianti255 – 2562ME → IK in strain: Bangladesh-1975.
Natural varianti270 – 2701R → K in strain: Bangladesh-1975.
Natural varianti278 – 2781S → F in strain: Bangladesh-1975.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X69198 Genomic DNA. Translation: CAA48969.1. Different initiation.
L22579 Genomic DNA. Translation: AAA60776.1. Different initiation.
PIRiH36839.
T28466.
RefSeqiNP_042072.2. NC_001611.1.

Genome annotation databases

GeneIDi1486388.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X69198 Genomic DNA. Translation: CAA48969.1 . Different initiation.
L22579 Genomic DNA. Translation: AAA60776.1 . Different initiation.
PIRi H36839.
T28466.
RefSeqi NP_042072.2. NC_001611.1.

3D structure databases

ProteinModelPortali P33799.
SMRi P33799. Positions 2-282.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1486388.

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

Gene3Di 1.10.620.20. 1 hit.
InterProi IPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view ]
PANTHERi PTHR23409. PTHR23409. 1 hit.
Pfami PF00268. Ribonuc_red_sm. 1 hit.
[Graphical view ]
SUPFAMi SSF47240. SSF47240. 1 hit.
PROSITEi PS00368. RIBORED_SMALL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Analysis of the nucleotide sequence of a 43 kbp segment of the genome of variola virus India-1967 strain."
    Shchelkunov S.N., Blinov V.M., Resenchuk S.M., Totmenin A.V., Sandakhchiev L.S.
    Virus Res. 30:239-258(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: India-1967 / Isolate Ind3.
  2. "Genes of variola and vaccinia viruses necessary to overcome the host protective mechanisms."
    Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.
    FEBS Lett. 319:80-83(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: India-1967 / Isolate Ind3.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Bangladesh-1975.

Entry informationi

Entry nameiRIR2_VAR67
AccessioniPrimary (citable) accession number: P33799
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 14, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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