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Protein

Ribonucleoside-diphosphate reductase small chain

Gene

C8L

Organism
Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

Cofactori

Fe cationBy similarityNote: Binds 2 iron ions per subunit.By similarity

Pathway:iDNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701Iron 1PROSITE-ProRule annotation
Metal bindingi101 – 1011Iron 1PROSITE-ProRule annotation
Metal bindingi101 – 1011Iron 2By similarity
Metal bindingi104 – 1041Iron 1PROSITE-ProRule annotation
Active sitei108 – 1081PROSITE-ProRule annotation
Metal bindingi163 – 1631Iron 2By similarity
Metal bindingi197 – 1971Iron 2By similarity
Metal bindingi200 – 2001Iron 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase small chain (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase small subunit
Gene namesi
ORF Names:C8L, F4L
OrganismiVariola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus)
Taxonomic identifieri587200 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000002060 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 319319Ribonucleoside-diphosphate reductase small chainPRO_0000190499Add
BLAST

Expressioni

Inductioni

Expressed early in the viral replicative cycle.

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP33799.
SMRiP33799. Positions 2-282.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK10808.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
IPR030475. RNR_small_AS.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33799-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPILAKNPN RFVIFPIQYH DIWNMYKKAE ASFWTVEEVD ISKDINDWNK
60 70 80 90 100
LTPDEKYFIK HVLAFFAASD GIVNENLAER FCIEVQITEA RCFYGFQMAI
110 120 130 140 150
ENIHSEMYSL LIDTYVKDSN EKNYLFNAIE TMPCVKKKAD WAQKWIHDSA
160 170 180 190 200
GYGERLIAFA AVEGIFFSGS FASIFWLKKR GLMPGLTFSN ELISRDEGLH
210 220 230 240 250
CDFACLMFKH LLYPPSEETV RSIITDAVSI EQEFLTVALP VKLIGMNCEM
260 270 280 290 300
MKTYMEFVAD RLISELGFKR IYNVTNPSDF MENISLEGKT NFFEKRVGEY
310
QKMGVMSQED NHFSLDVDF
Length:319
Mass (Da):37,031
Last modified:February 1, 1994 - v1
Checksum:iE0D62D56C4894928
GO

Sequence cautioni

The sequence AAA60776.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA48969.1 differs from that shown. Reason: Erroneous initiation. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti151 – 1511G → S in strain: Bangladesh-1975.
Natural varianti255 – 2562ME → IK in strain: Bangladesh-1975.
Natural varianti270 – 2701R → K in strain: Bangladesh-1975.
Natural varianti278 – 2781S → F in strain: Bangladesh-1975.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69198 Genomic DNA. Translation: CAA48969.1. Different initiation.
L22579 Genomic DNA. Translation: AAA60776.1. Different initiation.
PIRiH36839.
T28466.
RefSeqiNP_042072.2. NC_001611.1.

Genome annotation databases

GeneIDi1486388.
KEGGivg:1486388.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69198 Genomic DNA. Translation: CAA48969.1. Different initiation.
L22579 Genomic DNA. Translation: AAA60776.1. Different initiation.
PIRiH36839.
T28466.
RefSeqiNP_042072.2. NC_001611.1.

3D structure databases

ProteinModelPortaliP33799.
SMRiP33799. Positions 2-282.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1486388.
KEGGivg:1486388.

Phylogenomic databases

KOiK10808.

Enzyme and pathway databases

UniPathwayiUPA00326.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
IPR030475. RNR_small_AS.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Analysis of the nucleotide sequence of a 43 kbp segment of the genome of variola virus India-1967 strain."
    Shchelkunov S.N., Blinov V.M., Resenchuk S.M., Totmenin A.V., Sandakhchiev L.S.
    Virus Res. 30:239-258(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: India-1967 / Isolate Ind3.
  2. "Genes of variola and vaccinia viruses necessary to overcome the host protective mechanisms."
    Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.
    FEBS Lett. 319:80-83(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: India-1967 / Isolate Ind3.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Bangladesh-1975.

Entry informationi

Entry nameiRIR2_VAR67
AccessioniPrimary (citable) accession number: P33799
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 1, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.