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Reviewed, UniProtKB/Swiss-Prot P33798 (DNLI_VARV)

Last modified June 16, 2009. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]
Gene names
Name: LIG
ORF Names: A50R, J4R
OrganismVariola virus
Taxonomic identifier10255 [NCBI]
Taxonomic lineageViruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

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Protein attributes

Sequence length552 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. It is not essential for viral replication and recombination.

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Cofactor

Divalent metal cations By similarity.

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 552552DNA ligase
PRO_0000059590

Sites

Active site2311N6-AMP-lysine intermediate By similarity
Metal binding2831Divalent metal cation 1 By similarity
Metal binding3771Divalent metal cation 2 By similarity
Binding site2291ATP By similarity
Binding site2361ATP By similarity
Binding site2511ATP By similarity
Binding site2831ATP By similarity
Binding site3171ATP By similarity
Binding site3931ATP By similarity
Binding site3971ATP By similarity

Experimental info

Sequence conflict1941D → N in AAA60905. Ref.4

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Sequences

Sequence LengthMass (Da)Tools
P33798-1 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 618EDFB5B92FCE7D

FASTA55263,382
        10         20         30         40         50         60 
MTSLREFRKL CCAIYHASGY KEKSKLIRDF ITDRDDKYLI IKLLLPGLDD RIYNMNDKQI 

        70         80         90        100        110        120 
IKIYSIIFKQ SQKDMLQDLG YGYIGDTIST FFKENTEIRP RNKSILTLED VDSFLTTLSS 

       130        140        150        160        170        180 
ITKESHQIKL LTDIASVCTC NDLKCVVMLI DKDLKIKAGP RYVLNAISPH AYDVFRKSNN 

       190        200        210        220        230        240 
LKEIIENESK QNLDSISVSV MTPINPMLAE SCDSVNKAFK KFPSGMFAEV KYDGERVQVH 

       250        260        270        280        290        300 
KNNNEFAFFS RNMKPVLSYK VDYLKEYIPK AFKKATSIVL DSEIVLVDEH NVQLPFGSLG 

       310        320        330        340        350        360 
IHKKKEYKNS NMCLFVFDCL YFDGFDMTDI PLYKRRSFLK DVMVEIPNRI VFSELTNISN 

       370        380        390        400        410        420 
ESQLTDVLDD ALTRKLEGLV LKDINGVYEP GKRRWLKIKR DYLNEGSMAD SADLVVLGAY 

       430        440        450        460        470        480 
YGKGAKGGIM AVFLMGCYDD ESGKWKTVTK CSGHDDNTLR VLQDQLTMVK INKDPKKIPE 

       490        500        510        520        530        540 
WLVVNKIYIP DFVVEDPKQS QIWEISGAEF TSSKSHTANG ISIRFPRFTR IREDKTWKES 

       550 
THLNDLVNLT KS

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References

[1]"Creation of a clone library of fragments from the natural variola virus and study of the structural and functional organization of viral genes from a circle of hosts."
Shchelkunov S.N., Marennikova S.S., Totmenin A.V., Blinov V.M., Chizhikov V.E., Gutorov V.V., Safronov P.F., Pozdnyakov S.G., Shelukhina E.M., Gashnikov P.V., Anjaparidze O.G., Sandakhchiev L.S.
Dokl. Akad. Nauk SSSR 321:402-406(1991) [PubMed: 1666548] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: India-1967 / Isolate Ind3.
[2]"Genes of variola and vaccinia viruses necessary to overcome the host protective mechanisms."
Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.
FEBS Lett. 319:80-83(1993) [PubMed: 8384129] [Abstract]
Cited for: COMPLETE GENOME.
Strain: India-1967 / Isolate Ind3.
[3]"Nucleotide sequence of 21.8 kbp of variola major virus strain Harvey and comparison with vaccinia virus."
Aguado B., Selmes I.P., Smith G.L.
J. Gen. Virol. 73:2887-2902(1992) [PubMed: 1331292] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Harvey.
[4]"Potential virulence determinants in terminal regions of variola smallpox virus genome."
Massung R.F., Esposito J.J., Liu L.I., Qi J., Utterback T.R., Knight J.C., Aubin L., Yuran T.E., Parsons J.M., Loparev V.N., Selivanov N.A., Cavallaro K.F., Kerlavage A.R., Mahy B.W.J., Venter J.C.
Nature 366:748-751(1993) [PubMed: 8264798] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Bangladesh-1975.

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Cross-references

Sequence databases

X69198 Genomic DNA. Translation: CAA49103.1.
X67118 Genomic DNA. Translation: CAA47545.1.
L22579 Genomic DNA. Translation: AAA60905.1.
PIRJQ1857. C36854.
T28595.
RefSeqNP_042206.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID1486450.

Enzyme and pathway databases

BRENDA6.5.1.1. 288297.

Family and domain databases

InterProIPR000977. DNA_ligase.
IPR012309. DNA_ligase_A_C.
IPR012310. DNA_ligase_A_M.
IPR012308. DNA_ligase_A_N.
IPR016059. DNA_ligase_CS.
IPR012340. NA-bd_OB-fold.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameDNLI_VARV
AccessionPrimary (citable) accession number: P33798
Secondary accession number(s): Q90028
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectVirus (Virus annotation project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents