P33790 (TRAG1_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein TraG

Cleaved into the following chain:

1. Protein TraG*

Gene names

Name:	traG
Ordered Locus Names:	ECOK12F099

Encoded on

Plasmid F

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	938 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Predicted

General annotation (Comments)

Function	Plays a crucial role in donor-recipient cell interactions. Required for two stages of the conjugation process: pilus biosynthesis and mating aggregate stabilization. May interact with TraN.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Post-translational modification	TraG* probably arises from the post-translational cleavage of TraG.
Caution	TraG is not responsible for the N-terminal acetylation of F pilin as stated by some authors.

Ontologies

Keywords
Biological process	Conjugation
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	conjugation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 938	938	Protein TraG		PRO_0000024501
	Chain	? – 938		Protein TraG*		PRO_0000024502
Regions
	Topological domain	1 – 53	53	Cytoplasmic Potential
	Transmembrane	54 – 73	20	Helical; Potential
	Topological domain	74 – 329	256	Periplasmic Potential
	Transmembrane	330 – 350	21	Helical; Potential
	Topological domain	351 – 412	62	Cytoplasmic Potential
	Transmembrane	413 – 433	21	Helical; Potential
	Topological domain	434 – 938	505	Periplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P33790 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 9E09C8402ACDFDDF	FASTA	938	102,472
	10 20 30 40 50 60 MNEVYVIAGG EWLRNNLNAI AAFMGTWTWD SIEKIALTLS VLAVAVMWVQ RHNVMDLLGW 70 80 90 100 110 120 VAVFVLISLL VNVRTSVQII DNSDLVKVHR VDNVPVGLAM PLSLTTRIGH AMVASYEMIF 130 140 150 160 170 180 TQPDSVTYSK TGMLFGANLI VKSTDFLSRN PEIINLFQDY VQNCVLGDIY LNHKYTLEDL 190 200 210 220 230 240 MASADPYTLI FSRPSPLRGV YDNNNNFITC KDASVTLKDR LNLDTKTGGK TWHYYVQQIF 250 260 270 280 290 300 GGRPDPDLLF RQLVSDSYSY FYGSSQSASQ IMRQNVTMNA LKEGITSNAA RNGDTASLVS 310 320 330 340 350 360 LATTSSMEKQ RLAHVSIGHV TMRNLPMVQT ILTGIAIGIF PLLILAAVFN KLTLSVLKGY 370 380 390 400 410 420 VFALMWLQTW PLLYAILNSA MTFYAKQNGA PVVLSELSQI QLKYSNLAST AGYLSAMIPP 430 440 450 460 470 480 LSWMMVKGLG AGFSSVYSHF ASSSISPTAS AAGSVVDGNY SYGNMQTENV NGFSWSTNST 490 500 510 520 530 540 TSFGQMMYQT GSGATATQTR DGNMVMDASG AMSRLPVGIN ATRQIAAAQQ EMAREASNRA 550 560 570 580 590 600 ESALHGFSSS IASAWNTLSQ FGSNRGSSDS VTGGADSTMS AQDSMMASRM RSAVESYAKA 610 620 630 640 650 660 HNISNEQATR ELASRSTNAS LGLYGDAYAK GHLGISVLGN GGGVGLQAGA KASIDGSDLD 670 680 690 700 710 720 SHEASSGSRA SHDARHDIDA RATQDFKEAS DYFTSRKVSE SGSHTDNNAD SRVDQLSAAL 730 740 750 760 770 780 NSAKQSYDQY TTNMTRSHEY AEMASRTESM SGQMSEDLSQ QFAQYVMKNA PQDVEAILTN 790 800 810 820 830 840 TSSPEIAERR RAMAWSFVQE QVQPGVDNTW RESRRDIGKG MESVPSGGGS QDIIADHQGH 850 860 870 880 890 900 QAIIEQRTQD SNIRNDVKHQ VDNMVTEYRG NIGDTQNSIR GEENIVKGQY SELQNHHKTE 910 920 930 ALTQNNKYNE EKLAQERIPG ADSPKELLEK AKSYQHKE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the F plasmid bifunctional conjugation gene, traG." Firth N., Skurray R.A. Mol. Gen. Genet. 232:145-153(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the sequence and gene products of the transfer region of the F sex factor." Frost L.S., Ippen-Ihler K., Skurray R.A. Microbiol. Rev. 58:162-210(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete nucleotide sequence of the F plasmid: its implications for organization and diversification of plasmid genomes." Shimizu H., Saitoh Y., Suda Y., Uehara K., Sampei G., Mizobuchi K. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / CR63.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M59763 Genomic DNA. Translation: AAA98081.1. U01159 Genomic DNA. Translation: AAC44184.1. AP001918 Genomic DNA. Translation: BAA97969.1.
PIR	S20480.
RefSeq	NP_061478.1. NC_002483.1.
3D structure databases
ProteinModelPortal	P33790.
ModBase	Search...
Proteomic databases
PRIDE	P33790.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1263567.
Phylogenomic databases
OMA	SIRGEEN.
ProtClustDB	PRK13735.
Gene expression databases
Genevestigator	P33790.
Family and domain databases
InterPro	IPR012931. TraG_N. [Graphical view]
Pfam	PF07916. TraG_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRAG1_ECOLI

Accession

Primary (citable) accession number: P33790

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program