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P33790 (TRAG1_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Protein TraG

Cleaved into the following chain:

  1. Protein TraG*
Gene names
Name:traG
Ordered Locus Names:ECOK12F099
Encoded onPlasmid F
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length938 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existencePredicted

General annotation (Comments)

Function

Plays a crucial role in donor-recipient cell interactions. Required for two stages of the conjugation process: pilus biosynthesis and mating aggregate stabilization. May interact with TraN.

Subcellular location

Cell inner membrane; Multi-pass membrane protein.

Post-translational modification

TraG* probably arises from the post-translational cleavage of TraG.

Caution

TraG is not responsible for the N-terminal acetylation of F pilin as stated by some authors.

Ontologies

Keywords
   Biological processConjugation
   Cellular componentCell inner membrane
Cell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Technical termPlasmid
Gene Ontology (GO)
   Biological_processconjugation

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 938938Protein TraG
PRO_0000024501
Chain? – 938Protein TraG*PRO_0000024502

Regions

Topological domain1 – 5353Cytoplasmic Potential
Transmembrane54 – 7320Helical; Potential
Topological domain74 – 329256Periplasmic Potential
Transmembrane330 – 35021Helical; Potential
Topological domain351 – 41262Cytoplasmic Potential
Transmembrane413 – 43321Helical; Potential
Topological domain434 – 938505Periplasmic Potential

Sequences

Sequence LengthMass (Da)Tools
P33790 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 9E09C8402ACDFDDF

FASTA938102,472
        10         20         30         40         50         60 
MNEVYVIAGG EWLRNNLNAI AAFMGTWTWD SIEKIALTLS VLAVAVMWVQ RHNVMDLLGW 

        70         80         90        100        110        120 
VAVFVLISLL VNVRTSVQII DNSDLVKVHR VDNVPVGLAM PLSLTTRIGH AMVASYEMIF 

       130        140        150        160        170        180 
TQPDSVTYSK TGMLFGANLI VKSTDFLSRN PEIINLFQDY VQNCVLGDIY LNHKYTLEDL 

       190        200        210        220        230        240 
MASADPYTLI FSRPSPLRGV YDNNNNFITC KDASVTLKDR LNLDTKTGGK TWHYYVQQIF 

       250        260        270        280        290        300 
GGRPDPDLLF RQLVSDSYSY FYGSSQSASQ IMRQNVTMNA LKEGITSNAA RNGDTASLVS 

       310        320        330        340        350        360 
LATTSSMEKQ RLAHVSIGHV TMRNLPMVQT ILTGIAIGIF PLLILAAVFN KLTLSVLKGY 

       370        380        390        400        410        420 
VFALMWLQTW PLLYAILNSA MTFYAKQNGA PVVLSELSQI QLKYSNLAST AGYLSAMIPP 

       430        440        450        460        470        480 
LSWMMVKGLG AGFSSVYSHF ASSSISPTAS AAGSVVDGNY SYGNMQTENV NGFSWSTNST 

       490        500        510        520        530        540 
TSFGQMMYQT GSGATATQTR DGNMVMDASG AMSRLPVGIN ATRQIAAAQQ EMAREASNRA 

       550        560        570        580        590        600 
ESALHGFSSS IASAWNTLSQ FGSNRGSSDS VTGGADSTMS AQDSMMASRM RSAVESYAKA 

       610        620        630        640        650        660 
HNISNEQATR ELASRSTNAS LGLYGDAYAK GHLGISVLGN GGGVGLQAGA KASIDGSDLD 

       670        680        690        700        710        720 
SHEASSGSRA SHDARHDIDA RATQDFKEAS DYFTSRKVSE SGSHTDNNAD SRVDQLSAAL 

       730        740        750        760        770        780 
NSAKQSYDQY TTNMTRSHEY AEMASRTESM SGQMSEDLSQ QFAQYVMKNA PQDVEAILTN 

       790        800        810        820        830        840 
TSSPEIAERR RAMAWSFVQE QVQPGVDNTW RESRRDIGKG MESVPSGGGS QDIIADHQGH 

       850        860        870        880        890        900 
QAIIEQRTQD SNIRNDVKHQ VDNMVTEYRG NIGDTQNSIR GEENIVKGQY SELQNHHKTE 

       910        920        930 
ALTQNNKYNE EKLAQERIPG ADSPKELLEK AKSYQHKE 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of the F plasmid bifunctional conjugation gene, traG."
Firth N., Skurray R.A.
Mol. Gen. Genet. 232:145-153(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Analysis of the sequence and gene products of the transfer region of the F sex factor."
Frost L.S., Ippen-Ihler K., Skurray R.A.
Microbiol. Rev. 58:162-210(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Complete nucleotide sequence of the F plasmid: its implications for organization and diversification of plasmid genomes."
Shimizu H., Saitoh Y., Suda Y., Uehara K., Sampei G., Mizobuchi K.
Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / CR63.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M59763 Genomic DNA. Translation: AAA98081.1.
U01159 Genomic DNA. Translation: AAC44184.1.
AP001918 Genomic DNA. Translation: BAA97969.1.
PIRS20480.
RefSeqNP_061478.1. NC_002483.1.

3D structure databases

ProteinModelPortalP33790.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP33790.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1263567.

Phylogenomic databases

OMAQSASQIM.

Gene expression databases

GenevestigatorP33790.

Family and domain databases

InterProIPR012931. TraG_N.
[Graphical view]
PfamPF07916. TraG_N. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRAG1_ECOLI
AccessionPrimary (citable) accession number: P33790
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 14, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program